E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 714 |
| AciI | CCGC | 1 cut(s) | 325 |
| AclWI | GGATC | 1 cut(s) | 502 |
| AcoI | YGGCCR | 2 cut(s) | 268, 943 |
| AcsI | RAATTY | 2 cut(s) | 761, 816 |
| AcuI | CTGAAG | 2 cut(s) | 464, 947 |
| AfaI | GTAC | 2 cut(s) | 134, 580 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 50, 55, 56, 491 |
| AflII | CTTAAG | 1 cut(s) | 542 |
| AgsI | TTSAA | 5 cut(s) | 81, 199, 233, 586, 623 |
| AjnI | CCWGG | 2 cut(s) | 49, 821 |
| AluBI | AGCT | 3 cut(s) | 334, 442, 768 |
| AluI | AGCT | 3 cut(s) | 334, 442, 768 |
| Alw21I | GWGCWC | 2 cut(s) | 34, 506 |
| Alw26I | GTCTC | 1 cut(s) | 14 |
| Alw44I | GTGCAC | 1 cut(s) | 30 |
| AlwI | GGATC | 1 cut(s) | 502 |
| AoxI | GGCC | 2 cut(s) | 268, 943 |
| ApaLI | GTGCAC | 1 cut(s) | 30 |
| ApoI | RAATTY | 2 cut(s) | 761, 816 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 761 |
| AspLEI | GCGC | 1 cut(s) | 895 |
| BaeGI | GKGCMC | 2 cut(s) | 34, 113 |
| BalI | TGGCCA | 1 cut(s) | 270 |
| BbsI | GAAGAC | 1 cut(s) | 670 |
| Bbv12I | GWGCWC | 2 cut(s) | 34, 506 |
| BccI | CCATC | 4 cut(s) | 220, 566, 691, 860 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 443, 477 |
| BciT130I | CCWGG | 2 cut(s) | 51, 823 |
| BciVI | GTATCC | 1 cut(s) | 839 |
| BclI | TGATCA | 1 cut(s) | 550 |
| BcoDI | GTCTC | 1 cut(s) | 14 |
| BfmI | CTRYAG | 2 cut(s) | 433, 769 |
| BfoI | RGCGCY | 1 cut(s) | 896 |
| BfrI | CTTAAG | 1 cut(s) | 542 |
| BfuI | GTATCC | 1 cut(s) | 839 |
| BisI | GCNGC | 1 cut(s) | 325 |
| BlsI | GCNGC | 1 cut(s) | 326 |
| Bme1390I | CCNGG | 2 cut(s) | 51, 823 |
| BmiI | GGNNCC | 1 cut(s) | 708 |
| BmrFI | CCNGG | 2 cut(s) | 51, 823 |
| BmsI | GCATC | 1 cut(s) | 627 |
| BpiI | GAAGAC | 1 cut(s) | 670 |
| BpuEI | CTTGAG | 1 cut(s) | 125 |
| BsaJI | CCNNGG | 2 cut(s) | 49, 821 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 817, 847 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 50, 55, 56, 491 |
| Bse1I | ACTGG | 1 cut(s) | 946 |
| Bse3DI | GCAATG | 1 cut(s) | 12 |
| BseBI | CCWGG | 2 cut(s) | 51, 823 |
| BseDI | CCNNGG | 2 cut(s) | 49, 821 |
| BseGI | GGATG | 4 cut(s) | 231, 553, 642, 871 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 50, 55, 56, 491 |
| BseMI | GCAATG | 1 cut(s) | 12 |
| BseMII | CTCAG | 2 cut(s) | 489, 741 |
| BseNI | ACTGG | 1 cut(s) | 946 |
| BseSI | GKGCMC | 2 cut(s) | 34, 113 |
| BsgI | GTGCAG | 2 cut(s) | 103, 323 |
| BshFI | GGCC | 2 cut(s) | 270, 945 |
| BsiHKAI | GWGCWC | 2 cut(s) | 34, 506 |
| BsiSI | CCGG | 1 cut(s) | 946 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 50, 55, 56, 491 |
| BsmAI | GTCTC | 1 cut(s) | 14 |
| BsmI | GAATGC | 1 cut(s) | 194 |
| BsnI | GGCC | 2 cut(s) | 270, 945 |
| Bsp1286I | GDGCHC | 3 cut(s) | 34, 113, 506 |
| Bsp143I | GATC | 2 cut(s) | 494, 550 |
| BspACI | CCGC | 1 cut(s) | 325 |
| BspANI | GGCC | 2 cut(s) | 270, 945 |
| BspCNI | CTCAG | 2 cut(s) | 490, 742 |
| BspHI | TCATGA | 1 cut(s) | 415 |
| BspLI | GGNNCC | 1 cut(s) | 708 |
| BspPI | GGATC | 1 cut(s) | 502 |
| BspTI | CTTAAG | 1 cut(s) | 542 |
| BsrDI | GCAATG | 1 cut(s) | 12 |
| BsrI | ACTGG | 1 cut(s) | 946 |
| BssECI | CCNNGG | 2 cut(s) | 49, 821 |
| BssMI | GATC | 2 cut(s) | 494, 550 |
| Bst2UI | CCWGG | 2 cut(s) | 51, 823 |
| Bst4CI | ACNGT | 5 cut(s) | 25, 646, 669, 839, 860 |
| BstAFI | CTTAAG | 1 cut(s) | 542 |
| BstC8I | GCNNGC | 1 cut(s) | 440 |
| BstDEI | CTNAG | 2 cut(s) | 498, 750 |
| BstF5I | GGATG | 4 cut(s) | 231, 553, 642, 871 |
| BstH2I | RGCGCY | 1 cut(s) | 896 |
| BstHHI | GCGC | 1 cut(s) | 895 |
| BstKTI | GATC | 2 cut(s) | 497, 553 |
| BstMAI | GTCTC | 1 cut(s) | 14 |
| BstMBI | GATC | 2 cut(s) | 494, 550 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 117, 321 |
| BstNI | CCWGG | 2 cut(s) | 51, 823 |
| BstNSI | RCATGY | 1 cut(s) | 568 |
| BstSCI | CCNGG | 2 cut(s) | 49, 821 |
| BstSFI | CTRYAG | 2 cut(s) | 433, 769 |
| BstSLI | GKGCMC | 2 cut(s) | 34, 113 |
| BstV2I | GAAGAC | 1 cut(s) | 670 |
| BstX2I | RGATCY | 1 cut(s) | 494 |
| BstYI | RGATCY | 1 cut(s) | 494 |
| BsuI | GTATCC | 1 cut(s) | 839 |
| BsuRI | GGCC | 2 cut(s) | 270, 945 |
| BtgZI | GCGATG | 1 cut(s) | 756 |
| BtsCI | GGATG | 4 cut(s) | 231, 553, 642, 871 |
| Cac8I | GCNNGC | 1 cut(s) | 440 |
| CciI | TCATGA | 1 cut(s) | 415 |
| CfoI | GCGC | 1 cut(s) | 895 |
| Csp6I | GTAC | 2 cut(s) | 133, 579 |
| CviQI | GTAC | 2 cut(s) | 133, 579 |
| DdeI | CTNAG | 2 cut(s) | 498, 750 |
| DpnI | GATC | 2 cut(s) | 496, 552 |
| DpnII | GATC | 2 cut(s) | 494, 550 |
| EaeI | YGGCCR | 2 cut(s) | 268, 943 |
| Eco32I | GATATC | 1 cut(s) | 520 |
| Eco57I | CTGAAG | 2 cut(s) | 464, 947 |
| EcoRI | GAATTC | 1 cut(s) | 816 |
| EcoRII | CCWGG | 2 cut(s) | 49, 821 |
| EcoRV | GATATC | 1 cut(s) | 520 |
| EcoT22I | ATGCAT | 2 cut(s) | 395, 570 |
| FbaI | TGATCA | 1 cut(s) | 550 |
| FblI | GTMKAC | 1 cut(s) | 714 |
| Fnu4HI | GCNGC | 1 cut(s) | 325 |
| FokI | GGATG | 4 cut(s) | 238, 560, 649, 878 |
| Fsp4HI | GCNGC | 1 cut(s) | 325 |
| GlaI | GCGC | 1 cut(s) | 894 |
| GluI | GCNGC | 1 cut(s) | 325 |
| HaeII | RGCGCY | 1 cut(s) | 896 |
| HaeIII | GGCC | 2 cut(s) | 270, 945 |
| HapII | CCGG | 1 cut(s) | 946 |
| HhaI | GCGC | 1 cut(s) | 895 |
| Hin6I | GCGC | 1 cut(s) | 893 |
| HinP1I | GCGC | 1 cut(s) | 893 |
| HincII | GTYRAC | 1 cut(s) | 562 |
| HindII | GTYRAC | 1 cut(s) | 562 |
| HinfI | GANTC | 4 cut(s) | 173, 374, 732, 870 |
| HpaII | CCGG | 1 cut(s) | 946 |
| Hpy166II | GTNNAC | 4 cut(s) | 32, 562, 579, 715 |
| Hpy188I | TCNGA | 3 cut(s) | 19, 286, 499 |
| Hpy188III | TCNNGA | 4 cut(s) | 416, 694, 854, 863 |
| Hpy8I | GTNNAC | 4 cut(s) | 32, 562, 579, 715 |
| HpyAV | CCTTC | 3 cut(s) | 227, 358, 592 |
| HpyCH4III | ACNGT | 5 cut(s) | 25, 646, 669, 839, 860 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 117, 321 |
| HpyF3I | CTNAG | 2 cut(s) | 498, 750 |
| HspAI | GCGC | 1 cut(s) | 893 |
| Ksp22I | TGATCA | 1 cut(s) | 550 |
| Kzo9I | GATC | 2 cut(s) | 494, 550 |
| LmnI | GCTCC | 1 cut(s) | 712 |
| LweI | GCATC | 1 cut(s) | 627 |
| MaeIII | GTNAC | 4 cut(s) | 99, 508, 833, 854 |
| MalI | GATC | 2 cut(s) | 496, 552 |
| MboI | GATC | 2 cut(s) | 494, 550 |
| MboII | GAAGA | 2 cut(s) | 675, 769 |
| MfeI | CAATTG | 1 cut(s) | 478 |
| MflI | RGATCY | 1 cut(s) | 494 |
| MhlI | GDGCHC | 3 cut(s) | 34, 113, 506 |
| MlsI | TGGCCA | 1 cut(s) | 270 |
| MluCI | AATT | 5 cut(s) | 307, 478, 652, 761, 816 |
| MluNI | TGGCCA | 1 cut(s) | 270 |
| MlyI | GAGTC | 1 cut(s) | 864 |
| MnlI | CCTC | 7 cut(s) | 147, 150, 230, 679, 745, 772, 927 |
| Mox20I | TGGCCA | 1 cut(s) | 270 |
| Mph1103I | ATGCAT | 2 cut(s) | 395, 570 |
| MroXI | GAANNNNTTC | 1 cut(s) | 761 |
| MscI | TGGCCA | 1 cut(s) | 270 |
| MseI | TTAA | 7 cut(s) | 36, 162, 534, 543, 600, 651, 803 |
| MslI | CAYNNNNRTG | 1 cut(s) | 594 |
| Msp20I | TGGCCA | 1 cut(s) | 270 |
| MspCI | CTTAAG | 1 cut(s) | 542 |
| MspI | CCGG | 1 cut(s) | 946 |
| MspR9I | CCNGG | 2 cut(s) | 51, 823 |
| MunI | CAATTG | 1 cut(s) | 478 |
| Mva1269I | GAATGC | 1 cut(s) | 194 |
| MvaI | CCWGG | 2 cut(s) | 51, 823 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 117, 321 |
| NdeII | GATC | 2 cut(s) | 494, 550 |
| NlaIV | GGNNCC | 1 cut(s) | 708 |
| NmuCI | GTSAC | 2 cut(s) | 833, 854 |
| NsiI | ATGCAT | 2 cut(s) | 395, 570 |
| NspI | RCATGY | 1 cut(s) | 568 |
| PagI | TCATGA | 1 cut(s) | 415 |
| PctI | GAATGC | 1 cut(s) | 194 |
| PdmI | GAANNNNTTC | 1 cut(s) | 761 |
| PfeI | GAWTC | 3 cut(s) | 173, 374, 732 |
| PkrI | GCNGC | 1 cut(s) | 326 |
| PleI | GAGTC | 1 cut(s) | 864 |
| PpsI | GAGTC | 1 cut(s) | 864 |
| Psp6I | CCWGG | 2 cut(s) | 49, 821 |
| PspGI | CCWGG | 2 cut(s) | 49, 821 |
| PspN4I | GGNNCC | 1 cut(s) | 708 |
| PsuI | RGATCY | 1 cut(s) | 494 |
| RsaI | GTAC | 2 cut(s) | 134, 580 |
| RsaNI | GTAC | 2 cut(s) | 133, 579 |
| RseI | CAYNNNNRTG | 1 cut(s) | 594 |
| SaqAI | TTAA | 7 cut(s) | 36, 162, 534, 543, 600, 651, 803 |
| SatI | GCNGC | 1 cut(s) | 325 |
| Sau3AI | GATC | 2 cut(s) | 494, 550 |
| SchI | GAGTC | 1 cut(s) | 864 |
| ScrFI | CCNGG | 2 cut(s) | 51, 823 |
| SduI | GDGCHC | 3 cut(s) | 34, 113, 506 |
| SfaNI | GCATC | 1 cut(s) | 627 |
| SfcI | CTRYAG | 2 cut(s) | 433, 769 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 594 |
| SmlI | CTYRAG | 2 cut(s) | 104, 542 |
| SmoI | CTYRAG | 2 cut(s) | 104, 542 |
| Sse9I | AATT | 5 cut(s) | 307, 478, 652, 761, 816 |
| SsiI | CCGC | 1 cut(s) | 325 |
| StyD4I | CCNGG | 2 cut(s) | 49, 821 |
| TaaI | ACNGT | 5 cut(s) | 25, 646, 669, 839, 860 |
| TaqI | TCGA | 1 cut(s) | 463 |
| TasI | AATT | 5 cut(s) | 307, 478, 652, 761, 816 |
| TauI | GCSGC | 1 cut(s) | 327 |
| TfiI | GAWTC | 3 cut(s) | 173, 374, 732 |
| Tru1I | TTAA | 7 cut(s) | 36, 162, 534, 543, 600, 651, 803 |
| Tru9I | TTAA | 7 cut(s) | 36, 162, 534, 543, 600, 651, 803 |
| TseFI | GTSAC | 2 cut(s) | 833, 854 |
| Tsp45I | GTSAC | 2 cut(s) | 833, 854 |
| TspDTI | ATGAA | 3 cut(s) | 134, 630, 724 |
| TspGWI | ACGGA | 1 cut(s) | 821 |
| Vha464I | CTTAAG | 1 cut(s) | 542 |
| VneI | GTGCAC | 1 cut(s) | 30 |
| XapI | RAATTY | 2 cut(s) | 761, 816 |
| XceI | RCATGY | 1 cut(s) | 568 |
| XmiI | GTMKAC | 1 cut(s) | 714 |
| XmnI | GAANNNNTTC | 1 cut(s) | 761 |
| Zsp2I | ATGCAT | 2 cut(s) | 395, 570 |