E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 453, 606 |
| AciI | CCGC | 1 cut(s) | 217 |
| AclWI | GGATC | 1 cut(s) | 394 |
| AcoI | YGGCCR | 2 cut(s) | 160, 835 |
| AcsI | RAATTY | 2 cut(s) | 653, 708 |
| AcuI | CTGAAG | 2 cut(s) | 356, 839 |
| AfaI | GTAC | 2 cut(s) | 134, 472 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 50, 55, 56, 383 |
| AflII | CTTAAG | 1 cut(s) | 434 |
| AgsI | TTSAA | 3 cut(s) | 81, 478, 515 |
| AjnI | CCWGG | 3 cut(s) | 49, 713, 753 |
| AluBI | AGCT | 4 cut(s) | 26, 226, 334, 660 |
| AluI | AGCT | 4 cut(s) | 26, 226, 334, 660 |
| Alw21I | GWGCWC | 2 cut(s) | 34, 398 |
| Alw26I | GTCTC | 1 cut(s) | 14 |
| Alw44I | GTGCAC | 1 cut(s) | 30 |
| AlwI | GGATC | 1 cut(s) | 394 |
| AoxI | GGCC | 2 cut(s) | 160, 835 |
| ApaLI | GTGCAC | 1 cut(s) | 30 |
| ApoI | RAATTY | 2 cut(s) | 653, 708 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 653 |
| AspLEI | GCGC | 1 cut(s) | 787 |
| BaeGI | GKGCMC | 2 cut(s) | 34, 113 |
| BalI | TGGCCA | 1 cut(s) | 162 |
| BbsI | GAAGAC | 1 cut(s) | 562 |
| Bbv12I | GWGCWC | 2 cut(s) | 34, 398 |
| BccI | CCATC | 3 cut(s) | 458, 583, 752 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 335, 369 |
| BciT130I | CCWGG | 3 cut(s) | 51, 715, 755 |
| BciVI | GTATCC | 1 cut(s) | 731 |
| BclI | TGATCA | 1 cut(s) | 442 |
| BcoDI | GTCTC | 1 cut(s) | 14 |
| BfmI | CTRYAG | 2 cut(s) | 325, 661 |
| BfoI | RGCGCY | 1 cut(s) | 788 |
| BfrI | CTTAAG | 1 cut(s) | 434 |
| BfuI | GTATCC | 1 cut(s) | 731 |
| BisI | GCNGC | 1 cut(s) | 217 |
| BlsI | GCNGC | 1 cut(s) | 218 |
| Bme1390I | CCNGG | 3 cut(s) | 51, 715, 755 |
| BmiI | GGNNCC | 1 cut(s) | 600 |
| BmrFI | CCNGG | 3 cut(s) | 51, 715, 755 |
| BmsI | GCATC | 1 cut(s) | 519 |
| BpiI | GAAGAC | 1 cut(s) | 562 |
| BpmI | CTGGAG | 1 cut(s) | 585 |
| BpuEI | CTTGAG | 1 cut(s) | 125 |
| BsaJI | CCNNGG | 2 cut(s) | 49, 713 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 709, 739 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 50, 55, 56, 383 |
| Bse1I | ACTGG | 2 cut(s) | 602, 838 |
| Bse3DI | GCAATG | 1 cut(s) | 12 |
| BseBI | CCWGG | 3 cut(s) | 51, 715, 755 |
| BseDI | CCNNGG | 2 cut(s) | 49, 713 |
| BseGI | GGATG | 3 cut(s) | 445, 534, 763 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 50, 55, 56, 383 |
| BseMI | GCAATG | 1 cut(s) | 12 |
| BseMII | CTCAG | 2 cut(s) | 381, 633 |
| BseNI | ACTGG | 2 cut(s) | 602, 838 |
| BseSI | GKGCMC | 2 cut(s) | 34, 113 |
| BsgI | GTGCAG | 2 cut(s) | 103, 215 |
| BshFI | GGCC | 2 cut(s) | 162, 837 |
| BsiHKAI | GWGCWC | 2 cut(s) | 34, 398 |
| BsiSI | CCGG | 1 cut(s) | 838 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 50, 55, 56, 383 |
| BsmAI | GTCTC | 1 cut(s) | 14 |
| BsnI | GGCC | 2 cut(s) | 162, 837 |
| Bsp1286I | GDGCHC | 3 cut(s) | 34, 113, 398 |
| Bsp143I | GATC | 2 cut(s) | 386, 442 |
| BspACI | CCGC | 1 cut(s) | 217 |
| BspANI | GGCC | 2 cut(s) | 162, 837 |
| BspCNI | CTCAG | 2 cut(s) | 382, 634 |
| BspHI | TCATGA | 1 cut(s) | 307 |
| BspLI | GGNNCC | 1 cut(s) | 600 |
| BspPI | GGATC | 1 cut(s) | 394 |
| BspTI | CTTAAG | 1 cut(s) | 434 |
| BsrDI | GCAATG | 1 cut(s) | 12 |
| BsrI | ACTGG | 2 cut(s) | 602, 838 |
| BssECI | CCNNGG | 2 cut(s) | 49, 713 |
| BssMI | GATC | 2 cut(s) | 386, 442 |
| Bst2UI | CCWGG | 3 cut(s) | 51, 715, 755 |
| Bst4CI | ACNGT | 3 cut(s) | 538, 561, 731 |
| BstAFI | CTTAAG | 1 cut(s) | 434 |
| BstC8I | GCNNGC | 1 cut(s) | 332 |
| BstDEI | CTNAG | 2 cut(s) | 390, 642 |
| BstF5I | GGATG | 3 cut(s) | 445, 534, 763 |
| BstH2I | RGCGCY | 1 cut(s) | 788 |
| BstHHI | GCGC | 1 cut(s) | 787 |
| BstKTI | GATC | 2 cut(s) | 389, 445 |
| BstMAI | GTCTC | 1 cut(s) | 14 |
| BstMBI | GATC | 2 cut(s) | 386, 442 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 117, 213 |
| BstNI | CCWGG | 3 cut(s) | 51, 715, 755 |
| BstNSI | RCATGY | 1 cut(s) | 460 |
| BstSCI | CCNGG | 3 cut(s) | 49, 713, 753 |
| BstSFI | CTRYAG | 2 cut(s) | 325, 661 |
| BstSLI | GKGCMC | 2 cut(s) | 34, 113 |
| BstV2I | GAAGAC | 1 cut(s) | 562 |
| BstX2I | RGATCY | 1 cut(s) | 386 |
| BstYI | RGATCY | 1 cut(s) | 386 |
| BsuI | GTATCC | 1 cut(s) | 731 |
| BsuRI | GGCC | 2 cut(s) | 162, 837 |
| BtgZI | GCGATG | 1 cut(s) | 648 |
| BtsCI | GGATG | 3 cut(s) | 445, 534, 763 |
| BtsIMutI | CAGTG | 1 cut(s) | 155 |
| Cac8I | GCNNGC | 1 cut(s) | 332 |
| CciI | TCATGA | 1 cut(s) | 307 |
| CfoI | GCGC | 1 cut(s) | 787 |
| Csp6I | GTAC | 2 cut(s) | 133, 471 |
| CviQI | GTAC | 2 cut(s) | 133, 471 |
| DdeI | CTNAG | 2 cut(s) | 390, 642 |
| DpnI | GATC | 2 cut(s) | 388, 444 |
| DpnII | GATC | 2 cut(s) | 386, 442 |
| EaeI | YGGCCR | 2 cut(s) | 160, 835 |
| Eco32I | GATATC | 1 cut(s) | 412 |
| Eco57I | CTGAAG | 2 cut(s) | 356, 839 |
| EcoRI | GAATTC | 1 cut(s) | 708 |
| EcoRII | CCWGG | 3 cut(s) | 49, 713, 753 |
| EcoRV | GATATC | 1 cut(s) | 412 |
| EcoT22I | ATGCAT | 2 cut(s) | 287, 462 |
| FbaI | TGATCA | 1 cut(s) | 442 |
| FblI | GTMKAC | 2 cut(s) | 453, 606 |
| Fnu4HI | GCNGC | 1 cut(s) | 217 |
| FokI | GGATG | 3 cut(s) | 452, 541, 770 |
| Fsp4HI | GCNGC | 1 cut(s) | 217 |
| GlaI | GCGC | 1 cut(s) | 786 |
| GluI | GCNGC | 1 cut(s) | 217 |
| GsuI | CTGGAG | 1 cut(s) | 585 |
| HaeII | RGCGCY | 1 cut(s) | 788 |
| HaeIII | GGCC | 2 cut(s) | 162, 837 |
| HapII | CCGG | 1 cut(s) | 838 |
| HhaI | GCGC | 1 cut(s) | 787 |
| Hin6I | GCGC | 1 cut(s) | 785 |
| HinP1I | GCGC | 1 cut(s) | 785 |
| HincII | GTYRAC | 1 cut(s) | 454 |
| HindII | GTYRAC | 1 cut(s) | 454 |
| HinfI | GANTC | 3 cut(s) | 266, 624, 762 |
| HpaII | CCGG | 1 cut(s) | 838 |
| Hpy166II | GTNNAC | 4 cut(s) | 32, 454, 471, 607 |
| Hpy188I | TCNGA | 3 cut(s) | 19, 178, 391 |
| Hpy188III | TCNNGA | 3 cut(s) | 308, 586, 746 |
| Hpy8I | GTNNAC | 4 cut(s) | 32, 454, 471, 607 |
| HpyAV | CCTTC | 2 cut(s) | 250, 484 |
| HpyCH4III | ACNGT | 3 cut(s) | 538, 561, 731 |
| HpyCH4V | TGCA | 9 cut(s) | 32, 94, 120, 183, 196, 285, 460, 521, 571 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 117, 213 |
| HpyF3I | CTNAG | 2 cut(s) | 390, 642 |
| HspAI | GCGC | 1 cut(s) | 785 |
| Ksp22I | TGATCA | 1 cut(s) | 442 |
| Kzo9I | GATC | 2 cut(s) | 386, 442 |
| LmnI | GCTCC | 1 cut(s) | 604 |
| LweI | GCATC | 1 cut(s) | 519 |
| MaeIII | GTNAC | 4 cut(s) | 99, 400, 725, 746 |
| MalI | GATC | 2 cut(s) | 388, 444 |
| MboI | GATC | 2 cut(s) | 386, 442 |
| MboII | GAAGA | 2 cut(s) | 567, 661 |
| MfeI | CAATTG | 1 cut(s) | 370 |
| MflI | RGATCY | 1 cut(s) | 386 |
| MhlI | GDGCHC | 3 cut(s) | 34, 113, 398 |
| MlsI | TGGCCA | 1 cut(s) | 162 |
| MluCI | AATT | 5 cut(s) | 199, 370, 544, 653, 708 |
| MluNI | TGGCCA | 1 cut(s) | 162 |
| MlyI | GAGTC | 1 cut(s) | 756 |
| MnlI | CCTC | 5 cut(s) | 147, 571, 637, 664, 819 |
| Mox20I | TGGCCA | 1 cut(s) | 162 |
| Mph1103I | ATGCAT | 2 cut(s) | 287, 462 |
| MroXI | GAANNNNTTC | 1 cut(s) | 653 |
| MscI | TGGCCA | 1 cut(s) | 162 |
| MseI | TTAA | 7 cut(s) | 36, 128, 426, 435, 492, 543, 695 |
| MslI | CAYNNNNRTG | 1 cut(s) | 486 |
| Msp20I | TGGCCA | 1 cut(s) | 162 |
| MspA1I | CMGCKG | 1 cut(s) | 26 |
| MspCI | CTTAAG | 1 cut(s) | 434 |
| MspI | CCGG | 1 cut(s) | 838 |
| MspR9I | CCNGG | 3 cut(s) | 51, 715, 755 |
| MunI | CAATTG | 1 cut(s) | 370 |
| MvaI | CCWGG | 3 cut(s) | 51, 715, 755 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 117, 213 |
| NdeII | GATC | 2 cut(s) | 386, 442 |
| NlaIV | GGNNCC | 1 cut(s) | 600 |
| NmuCI | GTSAC | 2 cut(s) | 725, 746 |
| NsiI | ATGCAT | 2 cut(s) | 287, 462 |
| NspI | RCATGY | 1 cut(s) | 460 |
| PagI | TCATGA | 1 cut(s) | 307 |
| PdmI | GAANNNNTTC | 1 cut(s) | 653 |
| PfeI | GAWTC | 2 cut(s) | 266, 624 |
| PkrI | GCNGC | 1 cut(s) | 218 |
| PleI | GAGTC | 1 cut(s) | 756 |
| PpsI | GAGTC | 1 cut(s) | 756 |
| Psp6I | CCWGG | 3 cut(s) | 49, 713, 753 |
| PspGI | CCWGG | 3 cut(s) | 49, 713, 753 |
| PspN4I | GGNNCC | 1 cut(s) | 600 |
| PsuI | RGATCY | 1 cut(s) | 386 |
| PvuII | CAGCTG | 1 cut(s) | 26 |
| RsaI | GTAC | 2 cut(s) | 134, 472 |
| RsaNI | GTAC | 2 cut(s) | 133, 471 |
| RseI | CAYNNNNRTG | 1 cut(s) | 486 |
| SalI | GTCGAC | 1 cut(s) | 452 |
| SaqAI | TTAA | 7 cut(s) | 36, 128, 426, 435, 492, 543, 695 |
| SatI | GCNGC | 1 cut(s) | 217 |
| Sau3AI | GATC | 2 cut(s) | 386, 442 |
| SchI | GAGTC | 1 cut(s) | 756 |
| ScrFI | CCNGG | 3 cut(s) | 51, 715, 755 |
| SduI | GDGCHC | 3 cut(s) | 34, 113, 398 |
| SfaNI | GCATC | 1 cut(s) | 519 |
| SfcI | CTRYAG | 2 cut(s) | 325, 661 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 486 |
| SmlI | CTYRAG | 2 cut(s) | 104, 434 |
| SmoI | CTYRAG | 2 cut(s) | 104, 434 |
| Sse9I | AATT | 5 cut(s) | 199, 370, 544, 653, 708 |
| SsiI | CCGC | 1 cut(s) | 217 |
| StyD4I | CCNGG | 3 cut(s) | 49, 713, 753 |
| TaaI | ACNGT | 3 cut(s) | 538, 561, 731 |
| TaqI | TCGA | 2 cut(s) | 355, 453 |
| TasI | AATT | 5 cut(s) | 199, 370, 544, 653, 708 |
| TauI | GCSGC | 1 cut(s) | 219 |
| TfiI | GAWTC | 2 cut(s) | 266, 624 |
| Tru1I | TTAA | 7 cut(s) | 36, 128, 426, 435, 492, 543, 695 |
| Tru9I | TTAA | 7 cut(s) | 36, 128, 426, 435, 492, 543, 695 |
| TscAI | CASTG | 1 cut(s) | 155 |
| TseFI | GTSAC | 2 cut(s) | 725, 746 |
| Tsp45I | GTSAC | 2 cut(s) | 725, 746 |
| TspDTI | ATGAA | 3 cut(s) | 134, 522, 616 |
| TspGWI | ACGGA | 1 cut(s) | 713 |
| TspRI | CASTG | 1 cut(s) | 155 |
| Vha464I | CTTAAG | 1 cut(s) | 434 |
| VneI | GTGCAC | 1 cut(s) | 30 |
| XapI | RAATTY | 2 cut(s) | 653, 708 |
| XceI | RCATGY | 1 cut(s) | 460 |
| XmiI | GTMKAC | 2 cut(s) | 453, 606 |
| XmnI | GAANNNNTTC | 1 cut(s) | 653 |
| Zsp2I | ATGCAT | 2 cut(s) | 287, 462 |