E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 148 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 203, 1332 |
| AccI | GTMKAC | 3 cut(s) | 509, 645, 1259 |
| AccII | CGCG | 2 cut(s) | 2101, 2103 |
| AciI | CCGC | 8 cut(s) | 14, 409, 1153, 1873, 1984, 2078, 2099, 2412 |
| AclWI | GGATC | 8 cut(s) | 385, 472, 485, 718, 871, 892, 2196, 2333 |
| AcoI | YGGCCR | 1 cut(s) | 15 |
| AcsI | RAATTY | 7 cut(s) | 335, 931, 1339, 1426, 1658, 1910, 2450 |
| AcuI | CTGAAG | 7 cut(s) | 297, 828, 882, 1050, 1405, 1602, 2372 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 203, 222, 969, 1332, 1352, 1691, 1951 |
| AflIII | ACRYGT | 1 cut(s) | 1725 |
| AjnI | CCWGG | 7 cut(s) | 480, 499, 879, 1189, 1510, 1950, 2200 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 413, 445, 565, 597 |
| AluBI | AGCT | 7 cut(s) | 122, 163, 612, 811, 1108, 1246, 2018 |
| AluI | AGCT | 7 cut(s) | 122, 163, 612, 811, 1108, 1246, 2018 |
| Alw21I | GWGCWC | 2 cut(s) | 43, 165 |
| Alw26I | GTCTC | 7 cut(s) | 265, 277, 1150, 1180, 1892, 1992, 2271 |
| AlwI | GGATC | 8 cut(s) | 385, 472, 485, 718, 871, 892, 2196, 2333 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 320, 811 |
| Ama87I | CYCGRG | 3 cut(s) | 215, 687, 1320 |
| AoxI | GGCC | 7 cut(s) | 15, 308, 497, 691, 1323, 1666, 2131 |
| ApeKI | GCWGC | 9 cut(s) | 122, 143, 249, 517, 729, 811, 893, 1243, 1454 |
| ApoI | RAATTY | 7 cut(s) | 335, 931, 1339, 1426, 1658, 1910, 2450 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 83, 115 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 958, 2454, 2465 |
| AspLEI | GCGC | 1 cut(s) | 2103 |
| AspS9I | GGNCC | 7 cut(s) | 194, 497, 691, 1316, 1323, 2132, 2419 |
| AsuHPI | GGTGA | 3 cut(s) | 128, 275, 1205 |
| AvaI | CYCGRG | 3 cut(s) | 215, 687, 1320 |
| AvaII | GGWCC | 3 cut(s) | 194, 1316, 2419 |
| BamHI | GGATCC | 1 cut(s) | 477 |
| BanII | GRGCYC | 3 cut(s) | 165, 786, 1972 |
| BbsI | GAAGAC | 2 cut(s) | 1411, 2458 |
| Bbv12I | GWGCWC | 2 cut(s) | 43, 165 |
| BbvCI | CCTCAGC | 1 cut(s) | 1050 |
| BbvI | GCAGC | 9 cut(s) | 109, 130, 236, 504, 741, 798, 880, 1255, 1441 |
| BccI | CCATC | 9 cut(s) | 239, 399, 653, 695, 1711, 2015, 2108, 2251, 2414 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 170, 204 |
| BciT130I | CCWGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| BcoDI | GTCTC | 7 cut(s) | 265, 277, 1150, 1180, 1892, 1992, 2271 |
| BfaI | CTAG | 4 cut(s) | 1109, 1169, 1611, 2013 |
| BfmI | CTRYAG | 2 cut(s) | 993, 1791 |
| BfuAI | ACCTGC | 1 cut(s) | 148 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 496 |
| BglII | AGATCT | 2 cut(s) | 65, 2064 |
| Bme1390I | CCNGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| Bme18I | GGWCC | 3 cut(s) | 194, 1316, 2419 |
| BmeT110I | CYCGRG | 3 cut(s) | 215, 687, 1320 |
| BmgT120I | GGNCC | 7 cut(s) | 194, 497, 691, 1316, 1323, 2132, 2419 |
| BmiI | GGNNCC | 5 cut(s) | 479, 692, 1357, 1863, 2421 |
| BmrFI | CCNGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| BmrI | ACTGGG | 1 cut(s) | 2237 |
| BmsI | GCATC | 7 cut(s) | 618, 1036, 1313, 1441, 1477, 1593, 2171 |
| BmuI | ACTGGG | 1 cut(s) | 2237 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1407 |
| BpiI | GAAGAC | 2 cut(s) | 1411, 2458 |
| BpmI | CTGGAG | 2 cut(s) | 1937, 2075 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1050 |
| BpuEI | CTTGAG | 2 cut(s) | 1004, 1527 |
| BsaI | GGTCTC | 2 cut(s) | 1180, 1992 |
| BsaJI | CCNNGG | 6 cut(s) | 216, 481, 880, 1319, 1780, 1951 |
| BsaWI | WCCGGW | 1 cut(s) | 1704 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 203, 222, 969, 1332, 1352, 1691, 1951 |
| Bse1I | ACTGG | 2 cut(s) | 1326, 2232 |
| BseBI | CCWGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| BseDI | CCNNGG | 6 cut(s) | 216, 481, 880, 1319, 1780, 1951 |
| BseGI | GGATG | 6 cut(s) | 210, 285, 706, 1341, 1608, 1775 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 203, 222, 969, 1332, 1352, 1691, 1951 |
| BseMII | CTCAG | 5 cut(s) | 1041, 1249, 1507, 1585, 2147 |
| BseNI | ACTGG | 2 cut(s) | 1326, 2232 |
| BseXI | GCAGC | 9 cut(s) | 109, 130, 236, 504, 741, 798, 880, 1255, 1441 |
| BsgI | GTGCAG | 2 cut(s) | 381, 1717 |
| Bsh1236I | CGCG | 2 cut(s) | 2101, 2103 |
| BshFI | GGCC | 7 cut(s) | 17, 310, 499, 693, 1325, 1668, 2133 |
| BsiHKAI | GWGCWC | 2 cut(s) | 43, 165 |
| BsiHKCI | CYCGRG | 3 cut(s) | 215, 687, 1320 |
| BsiSI | CCGG | 2 cut(s) | 192, 1705 |
| BslFI | GGGAC | 1 cut(s) | 379 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 203, 222, 969, 1332, 1352, 1691, 1951 |
| BsmAI | GTCTC | 7 cut(s) | 265, 277, 1150, 1180, 1892, 1992, 2271 |
| BsmBI | CGTCTC | 2 cut(s) | 265, 2271 |
| BsmFI | GGGAC | 1 cut(s) | 379 |
| BsnI | GGCC | 7 cut(s) | 17, 310, 499, 693, 1325, 1668, 2133 |
| Bso31I | GGTCTC | 2 cut(s) | 1180, 1992 |
| BsoBI | CYCGRG | 3 cut(s) | 215, 687, 1320 |
| Bsp1286I | GDGCHC | 4 cut(s) | 43, 165, 786, 1972 |
| BspACI | CCGC | 8 cut(s) | 14, 409, 1153, 1873, 1984, 2078, 2099, 2412 |
| BspANI | GGCC | 7 cut(s) | 17, 310, 499, 693, 1325, 1668, 2133 |
| BspCNI | CTCAG | 5 cut(s) | 1042, 1248, 1506, 1584, 2148 |
| BspFNI | CGCG | 2 cut(s) | 2101, 2103 |
| BspHI | TCATGA | 1 cut(s) | 1557 |
| BspLI | GGNNCC | 5 cut(s) | 479, 692, 1357, 1863, 2421 |
| BspMAI | CTGCAG | 1 cut(s) | 1795 |
| BspMI | ACCTGC | 1 cut(s) | 148 |
| BspPI | GGATC | 8 cut(s) | 385, 472, 485, 718, 871, 892, 2196, 2333 |
| BspQI | GCTCTTC | 1 cut(s) | 1138 |
| BspTNI | GGTCTC | 2 cut(s) | 1180, 1992 |
| BsrI | ACTGG | 2 cut(s) | 1326, 2232 |
| BssECI | CCNNGG | 6 cut(s) | 216, 481, 880, 1319, 1780, 1951 |
| BssNAI | GTATAC | 3 cut(s) | 510, 646, 1260 |
| BssT1I | CCWWGG | 1 cut(s) | 1780 |
| Bst1107I | GTATAC | 3 cut(s) | 510, 646, 1260 |
| Bst2UI | CCWGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| Bst4CI | ACNGT | 2 cut(s) | 1264, 1409 |
| Bst6I | CTCTTC | 3 cut(s) | 1138, 2040, 2391 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 811, 2168 |
| BstC8I | GCNNGC | 3 cut(s) | 614, 1106, 2131 |
| BstDEI | CTNAG | 7 cut(s) | 915, 964, 1050, 1235, 1493, 1571, 2156 |
| BstEII | GGTNACC | 1 cut(s) | 2147 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 967 |
| BstF5I | GGATG | 6 cut(s) | 210, 285, 706, 1341, 1608, 1775 |
| BstFNI | CGCG | 2 cut(s) | 2101, 2103 |
| BstHHI | GCGC | 1 cut(s) | 2103 |
| BstMAI | GTCTC | 7 cut(s) | 265, 277, 1150, 1180, 1892, 1992, 2271 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 496, 811, 845, 1240, 2168 |
| BstNI | CCWGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| BstNSI | RCATGY | 3 cut(s) | 1729, 2242, 2375 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1407 |
| BstPI | GGTNACC | 1 cut(s) | 2147 |
| BstSCI | CCNGG | 7 cut(s) | 480, 499, 879, 1189, 1510, 1950, 2200 |
| BstSFI | CTRYAG | 2 cut(s) | 993, 1791 |
| BstUI | CGCG | 2 cut(s) | 2101, 2103 |
| BstV1I | GCAGC | 9 cut(s) | 109, 130, 236, 504, 741, 798, 880, 1255, 1441 |
| BstV2I | GAAGAC | 2 cut(s) | 1411, 2458 |
| BstX2I | RGATCY | 6 cut(s) | 65, 477, 876, 884, 2064, 2188 |
| BstYI | RGATCY | 6 cut(s) | 65, 477, 876, 884, 2064, 2188 |
| BstZ17I | GTATAC | 3 cut(s) | 510, 646, 1260 |
| BsuRI | GGCC | 7 cut(s) | 17, 310, 499, 693, 1325, 1668, 2133 |
| BtgZI | GCGATG | 1 cut(s) | 418 |
| BtsCI | GGATG | 6 cut(s) | 210, 285, 706, 1341, 1608, 1775 |
| BtsI | GCAGTG | 2 cut(s) | 197, 2205 |
| BtsIMutI | CAGTG | 2 cut(s) | 197, 2205 |
| BveI | ACCTGC | 1 cut(s) | 148 |
| Cac8I | GCNNGC | 3 cut(s) | 614, 1106, 2131 |
| CaiI | CAGNNNCTG | 2 cut(s) | 320, 811 |
| CciI | TCATGA | 1 cut(s) | 1557 |
| CfoI | GCGC | 1 cut(s) | 2103 |
| Cfr13I | GGNCC | 7 cut(s) | 194, 497, 691, 1316, 1323, 2132, 2419 |
| CseI | GACGC | 1 cut(s) | 2448 |
| CsiI | ACCWGGT | 1 cut(s) | 1189 |
| DdeI | CTNAG | 7 cut(s) | 915, 964, 1050, 1235, 1493, 1571, 2156 |
| DraI | TTTAAA | 1 cut(s) | 1842 |
| EaeI | YGGCCR | 1 cut(s) | 15 |
| Eam1104I | CTCTTC | 3 cut(s) | 1138, 2040, 2391 |
| EarI | CTCTTC | 3 cut(s) | 1138, 2040, 2391 |
| Ecl136II | GAGCTC | 1 cut(s) | 163 |
| Eco130I | CCWWGG | 1 cut(s) | 1780 |
| Eco24I | GRGCYC | 3 cut(s) | 165, 786, 1972 |
| Eco31I | GGTCTC | 2 cut(s) | 1180, 1992 |
| Eco32I | GATATC | 2 cut(s) | 437, 460 |
| Eco47I | GGWCC | 3 cut(s) | 194, 1316, 2419 |
| Eco53kI | GAGCTC | 1 cut(s) | 163 |
| Eco57I | CTGAAG | 7 cut(s) | 297, 828, 882, 1050, 1405, 1602, 2372 |
| Eco88I | CYCGRG | 3 cut(s) | 215, 687, 1320 |
| Eco91I | GGTNACC | 1 cut(s) | 2147 |
| EcoICRI | GAGCTC | 1 cut(s) | 163 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 967 |
| EcoO109I | RGGNCCY | 2 cut(s) | 691, 2132 |
| EcoO65I | GGTNACC | 1 cut(s) | 2147 |
| EcoRI | GAATTC | 1 cut(s) | 2450 |
| EcoRII | CCWGG | 7 cut(s) | 480, 499, 879, 1189, 1510, 1950, 2200 |
| EcoRV | GATATC | 2 cut(s) | 437, 460 |
| EcoT14I | CCWWGG | 1 cut(s) | 1780 |
| EcoT22I | ATGCAT | 3 cut(s) | 357, 633, 2377 |
| EcoT38I | GRGCYC | 3 cut(s) | 165, 786, 1972 |
| ErhI | CCWWGG | 1 cut(s) | 1780 |
| Esp3I | CGTCTC | 2 cut(s) | 265, 2271 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2175, 2207 |
| FaqI | GGGAC | 1 cut(s) | 379 |
| FauI | CCCGC | 3 cut(s) | 1866, 2085, 2106 |
| FauNDI | CATATG | 2 cut(s) | 187, 1636 |
| FblI | GTMKAC | 3 cut(s) | 509, 645, 1259 |
| FokI | GGATG | 6 cut(s) | 197, 292, 713, 1348, 1615, 1762 |
| FriOI | GRGCYC | 3 cut(s) | 165, 786, 1972 |
| FspBI | CTAG | 4 cut(s) | 1109, 1169, 1611, 2013 |
| GlaI | GCGC | 1 cut(s) | 2102 |
| GsuI | CTGGAG | 2 cut(s) | 1937, 2075 |
| HaeIII | GGCC | 7 cut(s) | 17, 310, 499, 693, 1325, 1668, 2133 |
| HapII | CCGG | 2 cut(s) | 192, 1705 |
| HgaI | GACGC | 1 cut(s) | 2448 |
| HhaI | GCGC | 1 cut(s) | 2103 |
| Hin6I | GCGC | 1 cut(s) | 2101 |
| HinP1I | GCGC | 1 cut(s) | 2101 |
| HpaII | CCGG | 2 cut(s) | 192, 1705 |
| HphI | GGTGA | 3 cut(s) | 128, 275, 1205 |
| Hpy166II | GTNNAC | 5 cut(s) | 510, 646, 1260, 2236, 2419 |
| Hpy8I | GTNNAC | 5 cut(s) | 510, 646, 1260, 2236, 2419 |
| Hpy99I | CGWCG | 1 cut(s) | 2442 |
| HpyCH4III | ACNGT | 2 cut(s) | 1264, 1409 |
| HpyCH4IV | ACGT | 1 cut(s) | 1823 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 496, 811, 845, 1240, 2168 |
| HpyF3I | CTNAG | 7 cut(s) | 915, 964, 1050, 1235, 1493, 1571, 2156 |
| HpySE526I | ACGT | 1 cut(s) | 1823 |
| HspAI | GCGC | 1 cut(s) | 2101 |
| LguI | GCTCTTC | 1 cut(s) | 1138 |
| LmnI | GCTCC | 2 cut(s) | 38, 1361 |
| Lsp1109I | GCAGC | 9 cut(s) | 109, 130, 236, 504, 741, 798, 880, 1255, 1441 |
| LweI | GCATC | 7 cut(s) | 618, 1036, 1313, 1441, 1477, 1593, 2171 |
| MabI | ACCWGGT | 1 cut(s) | 1189 |
| MaeI | CTAG | 4 cut(s) | 1109, 1169, 1611, 2013 |
| MaeII | ACGT | 1 cut(s) | 1823 |
| MaeIII | GTNAC | 6 cut(s) | 154, 822, 1211, 1220, 2147, 2174 |
| MfeI | CAATTG | 1 cut(s) | 1361 |
| MflI | RGATCY | 6 cut(s) | 65, 477, 876, 884, 2064, 2188 |
| MhlI | GDGCHC | 4 cut(s) | 43, 165, 786, 1972 |
| MlyI | GAGTC | 6 cut(s) | 162, 590, 679, 988, 1886, 2208 |
| MmeI | TCCRAC | 2 cut(s) | 655, 956 |
| Mph1103I | ATGCAT | 3 cut(s) | 357, 633, 2377 |
| MroXI | GAANNNNTTC | 3 cut(s) | 958, 2454, 2465 |
| MslI | CAYNNNNRTG | 2 cut(s) | 525, 1623 |
| MspA1I | CMGCKG | 1 cut(s) | 2414 |
| MspI | CCGG | 2 cut(s) | 192, 1705 |
| MspR9I | CCNGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| MunI | CAATTG | 1 cut(s) | 1361 |
| MvaI | CCWGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| MvnI | CGCG | 2 cut(s) | 2101, 2103 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 496, 811, 845, 1240, 2168 |
| NdeI | CATATG | 2 cut(s) | 187, 1636 |
| NlaIV | GGNNCC | 5 cut(s) | 479, 692, 1357, 1863, 2421 |
| NmuCI | GTSAC | 3 cut(s) | 154, 822, 1211 |
| NsiI | ATGCAT | 3 cut(s) | 357, 633, 2377 |
| NspI | RCATGY | 3 cut(s) | 1729, 2242, 2375 |
| PagI | TCATGA | 1 cut(s) | 1557 |
| PciI | ACATGT | 1 cut(s) | 1725 |
| PciSI | GCTCTTC | 1 cut(s) | 1138 |
| PdmI | GAANNNNTTC | 3 cut(s) | 958, 2454, 2465 |
| PfeI | GAWTC | 5 cut(s) | 415, 1016, 1888, 2269, 2396 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 203, 1332 |
| PfoI | TCCNGGA | 1 cut(s) | 1510 |
| PleI | GAGTC | 6 cut(s) | 161, 590, 679, 987, 1885, 2208 |
| PpsI | GAGTC | 6 cut(s) | 161, 590, 679, 987, 1885, 2208 |
| PscI | ACATGT | 1 cut(s) | 1725 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1407 |
| Psp124BI | GAGCTC | 1 cut(s) | 165 |
| Psp6I | CCWGG | 7 cut(s) | 480, 499, 879, 1189, 1510, 1950, 2200 |
| PspEI | GGTNACC | 1 cut(s) | 2147 |
| PspGI | CCWGG | 7 cut(s) | 480, 499, 879, 1189, 1510, 1950, 2200 |
| PspN4I | GGNNCC | 5 cut(s) | 479, 692, 1357, 1863, 2421 |
| PspPI | GGNCC | 7 cut(s) | 194, 497, 691, 1316, 1323, 2132, 2419 |
| PstI | CTGCAG | 1 cut(s) | 1795 |
| PstNI | CAGNNNCTG | 2 cut(s) | 320, 811 |
| PsuI | RGATCY | 6 cut(s) | 65, 477, 876, 884, 2064, 2188 |
| RseI | CAYNNNNRTG | 2 cut(s) | 525, 1623 |
| SacI | GAGCTC | 1 cut(s) | 165 |
| SapI | GCTCTTC | 1 cut(s) | 1138 |
| Sau96I | GGNCC | 7 cut(s) | 194, 497, 691, 1316, 1323, 2132, 2419 |
| SchI | GAGTC | 6 cut(s) | 162, 590, 679, 988, 1886, 2208 |
| ScrFI | CCNGG | 7 cut(s) | 482, 501, 881, 1191, 1512, 1952, 2202 |
| SduI | GDGCHC | 4 cut(s) | 43, 165, 786, 1972 |
| SexAI | ACCWGGT | 1 cut(s) | 1189 |
| SfaNI | GCATC | 7 cut(s) | 618, 1036, 1313, 1441, 1477, 1593, 2171 |
| SfcI | CTRYAG | 2 cut(s) | 993, 1791 |
| SinI | GGWCC | 3 cut(s) | 194, 1316, 2419 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 525, 1623 |
| SmlI | CTYRAG | 2 cut(s) | 983, 1542 |
| SmoI | CTYRAG | 2 cut(s) | 983, 1542 |
| SsiI | CCGC | 8 cut(s) | 14, 409, 1153, 1873, 1984, 2078, 2099, 2412 |
| SspMI | CTAG | 4 cut(s) | 1109, 1169, 1611, 2013 |
| SstI | GAGCTC | 1 cut(s) | 165 |
| StyD4I | CCNGG | 7 cut(s) | 480, 499, 879, 1189, 1510, 1950, 2200 |
| StyI | CCWWGG | 1 cut(s) | 1780 |
| TaaI | ACNGT | 2 cut(s) | 1264, 1409 |
| TaiI | ACGT | 1 cut(s) | 1826 |
| TaqI | TCGA | 2 cut(s) | 1136, 1300 |
| TauI | GCSGC | 1 cut(s) | 17 |
| TfiI | GAWTC | 5 cut(s) | 415, 1016, 1888, 2269, 2396 |
| TscAI | CASTG | 2 cut(s) | 204, 2212 |
| TseFI | GTSAC | 3 cut(s) | 154, 822, 1211 |
| TseI | GCWGC | 9 cut(s) | 122, 143, 249, 517, 729, 811, 893, 1243, 1454 |
| Tsp45I | GTSAC | 3 cut(s) | 154, 822, 1211 |
| TspGWI | ACGGA | 1 cut(s) | 1615 |
| TspRI | CASTG | 2 cut(s) | 204, 2212 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 203, 1332 |
| VpaK11BI | GGWCC | 3 cut(s) | 194, 1316, 2419 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 967 |
| XapI | RAATTY | 7 cut(s) | 335, 931, 1339, 1426, 1658, 1910, 2450 |
| XceI | RCATGY | 3 cut(s) | 1729, 2242, 2375 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2413 |
| XmiI | GTMKAC | 3 cut(s) | 509, 645, 1259 |
| XmnI | GAANNNNTTC | 3 cut(s) | 958, 2454, 2465 |
| XspI | CTAG | 4 cut(s) | 1109, 1169, 1611, 2013 |
| Zsp2I | ATGCAT | 3 cut(s) | 357, 633, 2377 |