E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 414 |
| Acc65I | GGTACC | 1 cut(s) | 357 |
| AccB1I | GGYRCC | 1 cut(s) | 357 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 367 |
| AclWI | GGATC | 4 cut(s) | 136, 149, 772, 785 |
| AcoI | YGGCCR | 1 cut(s) | 229 |
| AcsI | RAATTY | 2 cut(s) | 193, 364 |
| AfaI | GTAC | 4 cut(s) | 190, 203, 359, 677 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 367, 452 |
| AflII | CTTAAG | 1 cut(s) | 404 |
| AflIII | ACRYGT | 1 cut(s) | 281 |
| AgsI | TTSAA | 6 cut(s) | 25, 48, 176, 246, 547, 613 |
| AjnI | CCWGG | 2 cut(s) | 7, 112 |
| AluBI | AGCT | 7 cut(s) | 169, 295, 317, 403, 658, 735, 749 |
| AluI | AGCT | 7 cut(s) | 169, 295, 317, 403, 658, 735, 749 |
| Alw21I | GWGCWC | 1 cut(s) | 751 |
| Alw26I | GTCTC | 1 cut(s) | 619 |
| AlwI | GGATC | 4 cut(s) | 136, 149, 772, 785 |
| AoxI | GGCC | 2 cut(s) | 229, 431 |
| ApeKI | GCWGC | 1 cut(s) | 415 |
| ApoI | RAATTY | 2 cut(s) | 193, 364 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 722 |
| Asp718I | GGTACC | 1 cut(s) | 357 |
| AsuC2I | CCSGG | 1 cut(s) | 815 |
| AsuHPI | GGTGA | 2 cut(s) | 488, 884 |
| AsuII | TTCGAA | 1 cut(s) | 149 |
| BalI | TGGCCA | 1 cut(s) | 231 |
| BamHI | GGATCC | 2 cut(s) | 141, 777 |
| BanI | GGYRCC | 1 cut(s) | 357 |
| BanII | GRGCYC | 1 cut(s) | 751 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 713, 745 |
| BauI | CACGAG | 1 cut(s) | 410 |
| Bbv12I | GWGCWC | 1 cut(s) | 751 |
| BbvI | GCAGC | 1 cut(s) | 427 |
| BceAI | ACGGC | 1 cut(s) | 90 |
| BciT130I | CCWGG | 2 cut(s) | 9, 114 |
| BclI | TGATCA | 1 cut(s) | 511 |
| BcnI | CCSGG | 1 cut(s) | 815 |
| BcoDI | GTCTC | 1 cut(s) | 619 |
| BfaI | CTAG | 1 cut(s) | 783 |
| BfmI | CTRYAG | 1 cut(s) | 394 |
| BfrI | CTTAAG | 1 cut(s) | 404 |
| BfuAI | ACCTGC | 1 cut(s) | 414 |
| BisI | GCNGC | 1 cut(s) | 416 |
| BlpI | GCTNAGC | 1 cut(s) | 313 |
| BlsI | GCNGC | 1 cut(s) | 417 |
| Bme1390I | CCNGG | 3 cut(s) | 9, 114, 815 |
| BmiI | GGNNCC | 6 cut(s) | 6, 143, 359, 669, 779, 848 |
| BmrFI | CCNGG | 3 cut(s) | 9, 114, 815 |
| BmsI | GCATC | 4 cut(s) | 299, 527, 588, 798 |
| BpmI | CTGGAG | 3 cut(s) | 30, 654, 888 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 313 |
| Bpu14I | TTCGAA | 1 cut(s) | 149 |
| BpuEI | CTTGAG | 2 cut(s) | 341, 872 |
| BpuMI | CCSGG | 1 cut(s) | 815 |
| BsaJI | CCNNGG | 2 cut(s) | 113, 842 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 367, 452 |
| Bse118I | RCCGGY | 1 cut(s) | 354 |
| Bse1I | ACTGG | 2 cut(s) | 671, 907 |
| Bse3DI | GCAATG | 1 cut(s) | 418 |
| BseBI | CCWGG | 2 cut(s) | 9, 114 |
| BseDI | CCNNGG | 2 cut(s) | 113, 842 |
| BseGI | GGATG | 4 cut(s) | 542, 603, 789, 837 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 367, 452 |
| BseMI | GCAATG | 1 cut(s) | 418 |
| BseMII | CTCAG | 2 cut(s) | 248, 450 |
| BseNI | ACTGG | 2 cut(s) | 671, 907 |
| BseXI | GCAGC | 1 cut(s) | 427 |
| BseYI | CCCAGC | 1 cut(s) | 658 |
| BsgI | GTGCAG | 2 cut(s) | 141, 284 |
| BshFI | GGCC | 2 cut(s) | 231, 433 |
| BshNI | GGYRCC | 1 cut(s) | 357 |
| BsiHKAI | GWGCWC | 1 cut(s) | 751 |
| BsiSI | CCGG | 2 cut(s) | 355, 814 |
| BslFI | GGGAC | 2 cut(s) | 165, 830 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 367, 452 |
| BsmAI | GTCTC | 1 cut(s) | 619 |
| BsmFI | GGGAC | 2 cut(s) | 165, 830 |
| BsmI | GAATGC | 1 cut(s) | 683 |
| BsnI | GGCC | 2 cut(s) | 231, 433 |
| Bsp119I | TTCGAA | 1 cut(s) | 149 |
| Bsp1286I | GDGCHC | 1 cut(s) | 751 |
| Bsp1407I | TGTACA | 2 cut(s) | 188, 675 |
| Bsp143I | GATC | 3 cut(s) | 141, 511, 777 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 313 |
| BspANI | GGCC | 2 cut(s) | 231, 433 |
| BspCNI | CTCAG | 2 cut(s) | 249, 451 |
| BspHI | TCATGA | 1 cut(s) | 376 |
| BspLI | GGNNCC | 6 cut(s) | 6, 143, 359, 669, 779, 848 |
| BspMI | ACCTGC | 1 cut(s) | 414 |
| BspPI | GGATC | 4 cut(s) | 136, 149, 772, 785 |
| BspT104I | TTCGAA | 1 cut(s) | 149 |
| BspT107I | GGYRCC | 1 cut(s) | 357 |
| BspTI | CTTAAG | 1 cut(s) | 404 |
| BsrDI | GCAATG | 1 cut(s) | 418 |
| BsrFI | RCCGGY | 1 cut(s) | 354 |
| BsrGI | TGTACA | 2 cut(s) | 188, 675 |
| BsrI | ACTGG | 2 cut(s) | 671, 907 |
| BssAI | RCCGGY | 1 cut(s) | 354 |
| BssECI | CCNNGG | 2 cut(s) | 113, 842 |
| BssMI | GATC | 3 cut(s) | 141, 511, 777 |
| BssSI | CACGAG | 1 cut(s) | 410 |
| BssT1I | CCWWGG | 1 cut(s) | 842 |
| Bst2BI | CACGAG | 1 cut(s) | 410 |
| Bst2UI | CCWGG | 2 cut(s) | 9, 114 |
| Bst4CI | ACNGT | 6 cut(s) | 554, 607, 617, 630, 800, 821 |
| BstAFI | CTTAAG | 1 cut(s) | 404 |
| BstAUI | TGTACA | 2 cut(s) | 188, 675 |
| BstBI | TTCGAA | 1 cut(s) | 149 |
| BstC8I | GCNNGC | 1 cut(s) | 401 |
| BstDEI | CTNAG | 3 cut(s) | 257, 313, 459 |
| BstF5I | GGATG | 4 cut(s) | 542, 603, 789, 837 |
| BstKTI | GATC | 3 cut(s) | 144, 514, 780 |
| BstMAI | GTCTC | 1 cut(s) | 619 |
| BstMBI | GATC | 3 cut(s) | 141, 511, 777 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 415 |
| BstNI | CCWGG | 2 cut(s) | 9, 114 |
| BstNSI | RCATGY | 2 cut(s) | 276, 285 |
| BstSCI | CCNGG | 3 cut(s) | 7, 112, 813 |
| BstSFI | CTRYAG | 1 cut(s) | 394 |
| BstV1I | GCAGC | 1 cut(s) | 427 |
| BstX2I | RGATCY | 2 cut(s) | 141, 777 |
| BstYI | RGATCY | 2 cut(s) | 141, 777 |
| BsuRI | GGCC | 2 cut(s) | 231, 433 |
| BtsCI | GGATG | 4 cut(s) | 542, 603, 789, 837 |
| BtsI | GCAGTG | 1 cut(s) | 22 |
| BtsIMutI | CAGTG | 3 cut(s) | 22, 224, 678 |
| BveI | ACCTGC | 1 cut(s) | 414 |
| Cac8I | GCNNGC | 1 cut(s) | 401 |
| CciI | TCATGA | 1 cut(s) | 376 |
| Cfr10I | RCCGGY | 1 cut(s) | 354 |
| Csp6I | GTAC | 4 cut(s) | 189, 202, 358, 676 |
| CviQI | GTAC | 4 cut(s) | 189, 202, 358, 676 |
| DdeI | CTNAG | 3 cut(s) | 257, 313, 459 |
| DpnI | GATC | 3 cut(s) | 143, 513, 779 |
| DpnII | GATC | 3 cut(s) | 141, 511, 777 |
| EaeI | YGGCCR | 1 cut(s) | 229 |
| Ecl136II | GAGCTC | 1 cut(s) | 749 |
| Eco130I | CCWWGG | 1 cut(s) | 842 |
| Eco147I | AGGCCT | 1 cut(s) | 433 |
| Eco24I | GRGCYC | 1 cut(s) | 751 |
| Eco32I | GATATC | 1 cut(s) | 481 |
| Eco53kI | GAGCTC | 1 cut(s) | 749 |
| EcoICRI | GAGCTC | 1 cut(s) | 749 |
| EcoRII | CCWGG | 2 cut(s) | 7, 112 |
| EcoRV | GATATC | 1 cut(s) | 481 |
| EcoT14I | CCWWGG | 1 cut(s) | 842 |
| EcoT22I | ATGCAT | 1 cut(s) | 531 |
| EcoT38I | GRGCYC | 1 cut(s) | 751 |
| ErhI | CCWWGG | 1 cut(s) | 842 |
| FaqI | GGGAC | 2 cut(s) | 165, 830 |
| FbaI | TGATCA | 1 cut(s) | 511 |
| Fnu4HI | GCNGC | 1 cut(s) | 416 |
| FokI | GGATG | 4 cut(s) | 549, 610, 776, 824 |
| FriOI | GRGCYC | 1 cut(s) | 751 |
| Fsp4HI | GCNGC | 1 cut(s) | 416 |
| FspBI | CTAG | 1 cut(s) | 783 |
| GluI | GCNGC | 1 cut(s) | 416 |
| GsaI | CCCAGC | 1 cut(s) | 662 |
| GsuI | CTGGAG | 3 cut(s) | 30, 654, 888 |
| HaeIII | GGCC | 2 cut(s) | 231, 433 |
| HapII | CCGG | 2 cut(s) | 355, 814 |
| HindIII | AAGCTT | 1 cut(s) | 401 |
| HinfI | GANTC | 4 cut(s) | 38, 335, 831, 909 |
| HpaII | CCGG | 2 cut(s) | 355, 814 |
| HphI | GGTGA | 2 cut(s) | 488, 884 |
| Hpy166II | GTNNAC | 3 cut(s) | 163, 638, 676 |
| Hpy188I | TCNGA | 2 cut(s) | 225, 460 |
| Hpy188III | TCNNGA | 6 cut(s) | 173, 320, 377, 824, 867, 913 |
| Hpy8I | GTNNAC | 3 cut(s) | 163, 638, 676 |
| HpyAV | CCTTC | 2 cut(s) | 553, 698 |
| HpyCH4III | ACNGT | 6 cut(s) | 554, 607, 617, 630, 800, 821 |
| HpyCH4V | TGCA | 7 cut(s) | 122, 252, 265, 332, 423, 529, 540 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 415 |
| HpyF3I | CTNAG | 3 cut(s) | 257, 313, 459 |
| KpnI | GGTACC | 1 cut(s) | 361 |
| Ksp22I | TGATCA | 1 cut(s) | 511 |
| Kzo9I | GATC | 3 cut(s) | 141, 511, 777 |
| LmnI | GCTCC | 3 cut(s) | 10, 673, 746 |
| Lsp1109I | GCAGC | 1 cut(s) | 427 |
| LweI | GCATC | 4 cut(s) | 299, 527, 588, 798 |
| MaeI | CTAG | 1 cut(s) | 783 |
| MaeIII | GTNAC | 6 cut(s) | 299, 469, 728, 794, 800, 898 |
| MalI | GATC | 3 cut(s) | 143, 513, 779 |
| MboI | GATC | 3 cut(s) | 141, 511, 777 |
| MboII | GAAGA | 1 cut(s) | 730 |
| MflI | RGATCY | 2 cut(s) | 141, 777 |
| MhlI | GDGCHC | 1 cut(s) | 751 |
| MlsI | TGGCCA | 1 cut(s) | 231 |
| MluCI | AATT | 5 cut(s) | 43, 193, 210, 364, 761 |
| MluNI | TGGCCA | 1 cut(s) | 231 |
| MlyI | GAGTC | 2 cut(s) | 344, 825 |
| MnlI | CCTC | 9 cut(s) | 5, 58, 216, 571, 640, 706, 860, 863, 913 |
| Mox20I | TGGCCA | 1 cut(s) | 231 |
| Mph1103I | ATGCAT | 1 cut(s) | 531 |
| MroXI | GAANNNNTTC | 1 cut(s) | 722 |
| MscI | TGGCCA | 1 cut(s) | 231 |
| MseI | TTAA | 5 cut(s) | 69, 90, 197, 405, 764 |
| MslI | CAYNNNNRTG | 1 cut(s) | 555 |
| Msp20I | TGGCCA | 1 cut(s) | 231 |
| MspA1I | CMGCKG | 1 cut(s) | 658 |
| MspCI | CTTAAG | 1 cut(s) | 404 |
| MspI | CCGG | 2 cut(s) | 355, 814 |
| MspR9I | CCNGG | 3 cut(s) | 9, 114, 815 |
| Mva1269I | GAATGC | 1 cut(s) | 683 |
| MvaI | CCWGG | 2 cut(s) | 9, 114 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 415 |
| NciI | CCSGG | 1 cut(s) | 815 |
| NdeII | GATC | 3 cut(s) | 141, 511, 777 |
| NlaIV | GGNNCC | 6 cut(s) | 6, 143, 359, 669, 779, 848 |
| NmuCI | GTSAC | 3 cut(s) | 794, 800, 898 |
| NsiI | ATGCAT | 1 cut(s) | 531 |
| NspI | RCATGY | 2 cut(s) | 276, 285 |
| NspV | TTCGAA | 1 cut(s) | 149 |
| PagI | TCATGA | 1 cut(s) | 376 |
| PceI | AGGCCT | 1 cut(s) | 433 |
| PciI | ACATGT | 1 cut(s) | 281 |
| PctI | GAATGC | 1 cut(s) | 683 |
| PdmI | GAANNNNTTC | 1 cut(s) | 722 |
| PfeI | GAWTC | 2 cut(s) | 38, 909 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 367 |
| PfoI | TCCNGGA | 2 cut(s) | 7, 813 |
| PkrI | GCNGC | 1 cut(s) | 417 |
| PleI | GAGTC | 2 cut(s) | 343, 825 |
| PpsI | GAGTC | 2 cut(s) | 343, 825 |
| PscI | ACATGT | 1 cut(s) | 281 |
| Psp124BI | GAGCTC | 1 cut(s) | 751 |
| Psp6I | CCWGG | 2 cut(s) | 7, 112 |
| PspFI | CCCAGC | 1 cut(s) | 658 |
| PspGI | CCWGG | 2 cut(s) | 7, 112 |
| PspN4I | GGNNCC | 6 cut(s) | 6, 143, 359, 669, 779, 848 |
| PsuI | RGATCY | 2 cut(s) | 141, 777 |
| PvuII | CAGCTG | 1 cut(s) | 658 |
| RsaI | GTAC | 4 cut(s) | 190, 203, 359, 677 |
| RsaNI | GTAC | 4 cut(s) | 189, 202, 358, 676 |
| RseI | CAYNNNNRTG | 1 cut(s) | 555 |
| SacI | GAGCTC | 1 cut(s) | 751 |
| SaqAI | TTAA | 5 cut(s) | 69, 90, 197, 405, 764 |
| SatI | GCNGC | 1 cut(s) | 416 |
| Sau3AI | GATC | 3 cut(s) | 141, 511, 777 |
| SchI | GAGTC | 2 cut(s) | 344, 825 |
| ScrFI | CCNGG | 3 cut(s) | 9, 114, 815 |
| SduI | GDGCHC | 1 cut(s) | 751 |
| SfaNI | GCATC | 4 cut(s) | 299, 527, 588, 798 |
| SfcI | CTRYAG | 1 cut(s) | 394 |
| SfuI | TTCGAA | 1 cut(s) | 149 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 555 |
| SmlI | CTYRAG | 3 cut(s) | 320, 404, 887 |
| SmoI | CTYRAG | 3 cut(s) | 320, 404, 887 |
| Sse9I | AATT | 5 cut(s) | 43, 193, 210, 364, 761 |
| SseBI | AGGCCT | 1 cut(s) | 433 |
| SspMI | CTAG | 1 cut(s) | 783 |
| SstI | GAGCTC | 1 cut(s) | 751 |
| StuI | AGGCCT | 1 cut(s) | 433 |
| StyD4I | CCNGG | 3 cut(s) | 7, 112, 813 |
| StyI | CCWWGG | 1 cut(s) | 842 |
| TaaI | ACNGT | 6 cut(s) | 554, 607, 617, 630, 800, 821 |
| TaqI | TCGA | 4 cut(s) | 149, 522, 582, 645 |
| TasI | AATT | 5 cut(s) | 43, 193, 210, 364, 761 |
| TatI | WGTACW | 2 cut(s) | 188, 675 |
| TfiI | GAWTC | 2 cut(s) | 38, 909 |
| Tru1I | TTAA | 5 cut(s) | 69, 90, 197, 405, 764 |
| Tru9I | TTAA | 5 cut(s) | 69, 90, 197, 405, 764 |
| TscAI | CASTG | 3 cut(s) | 22, 224, 678 |
| TseFI | GTSAC | 3 cut(s) | 794, 800, 898 |
| TseI | GCWGC | 1 cut(s) | 415 |
| Tsp45I | GTSAC | 3 cut(s) | 794, 800, 898 |
| TspDTI | ATGAA | 2 cut(s) | 203, 488 |
| TspGWI | ACGGA | 4 cut(s) | 549, 772, 782, 843 |
| TspRI | CASTG | 3 cut(s) | 22, 224, 678 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 367 |
| Vha464I | CTTAAG | 1 cut(s) | 404 |
| XapI | RAATTY | 2 cut(s) | 193, 364 |
| XceI | RCATGY | 2 cut(s) | 276, 285 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 152 |
| XmnI | GAANNNNTTC | 1 cut(s) | 722 |
| XspI | CTAG | 1 cut(s) | 783 |
| Zsp2I | ATGCAT | 1 cut(s) | 531 |