E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 191 |
| AciI | CCGC | 2 cut(s) | 43, 154 |
| AclWI | GGATC | 1 cut(s) | 80 |
| AcoI | YGGCCR | 2 cut(s) | 64, 105 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 95 |
| AflII | CTTAAG | 1 cut(s) | 113 |
| AjiI | CACGTC | 1 cut(s) | 61 |
| AluBI | AGCT | 4 cut(s) | 10, 94, 112, 174 |
| AluI | AGCT | 4 cut(s) | 10, 94, 112, 174 |
| Alw21I | GWGCWC | 1 cut(s) | 141 |
| AlwI | GGATC | 1 cut(s) | 80 |
| AoxI | GGCC | 2 cut(s) | 64, 105 |
| ApeKI | GCWGC | 1 cut(s) | 40 |
| AspLEI | GCGC | 1 cut(s) | 192 |
| Bbv12I | GWGCWC | 1 cut(s) | 141 |
| BbvI | GCAGC | 1 cut(s) | 27 |
| BfrI | CTTAAG | 1 cut(s) | 113 |
| BisI | GCNGC | 1 cut(s) | 41 |
| BlsI | GCNGC | 1 cut(s) | 42 |
| BmgBI | CACGTC | 1 cut(s) | 61 |
| BsaJI | CCNNGG | 1 cut(s) | 35 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 95 |
| BseDI | CCNNGG | 1 cut(s) | 35 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 95 |
| BseXI | GCAGC | 1 cut(s) | 27 |
| Bsh1285I | CGRYCG | 1 cut(s) | 181 |
| BshFI | GGCC | 2 cut(s) | 66, 107 |
| BsiEI | CGRYCG | 1 cut(s) | 181 |
| BsiHKAI | GWGCWC | 1 cut(s) | 141 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 95 |
| BsnI | GGCC | 2 cut(s) | 66, 107 |
| Bsp1286I | GDGCHC | 1 cut(s) | 141 |
| Bsp143I | GATC | 2 cut(s) | 85, 178 |
| Bsp19I | CCATGG | 1 cut(s) | 35 |
| BspACI | CCGC | 2 cut(s) | 43, 154 |
| BspANI | GGCC | 2 cut(s) | 66, 107 |
| BspPI | GGATC | 1 cut(s) | 80 |
| BspTI | CTTAAG | 1 cut(s) | 113 |
| BssECI | CCNNGG | 1 cut(s) | 35 |
| BssMI | GATC | 2 cut(s) | 85, 178 |
| BssT1I | CCWWGG | 1 cut(s) | 35 |
| BstAFI | CTTAAG | 1 cut(s) | 113 |
| BstC8I | GCNNGC | 3 cut(s) | 68, 133, 137 |
| BstDSI | CCRYGG | 1 cut(s) | 35 |
| BstHHI | GCGC | 1 cut(s) | 192 |
| BstKTI | GATC | 2 cut(s) | 88, 181 |
| BstMBI | GATC | 2 cut(s) | 85, 178 |
| BstMCI | CGRYCG | 1 cut(s) | 181 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 46 |
| BstV1I | GCAGC | 1 cut(s) | 27 |
| BsuRI | GGCC | 2 cut(s) | 66, 107 |
| BtgI | CCRYGG | 1 cut(s) | 35 |
| BtrI | CACGTC | 1 cut(s) | 61 |
| Cac8I | GCNNGC | 3 cut(s) | 68, 133, 137 |
| CfoI | GCGC | 1 cut(s) | 192 |
| CseI | GACGC | 1 cut(s) | 55 |
| CviAII | CATG | 2 cut(s) | 36, 187 |
| CviJI | RGCY | 9 cut(s) | 10, 40, 66, 94, 107, 112, 131, 174, 184 |
| CviKI_1 | RGCY | 9 cut(s) | 10, 40, 66, 94, 107, 112, 131, 174, 184 |
| DpnI | GATC | 2 cut(s) | 87, 180 |
| DpnII | GATC | 2 cut(s) | 85, 178 |
| EaeI | YGGCCR | 2 cut(s) | 64, 105 |
| Eco130I | CCWWGG | 1 cut(s) | 35 |
| EcoT14I | CCWWGG | 1 cut(s) | 35 |
| ErhI | CCWWGG | 1 cut(s) | 35 |
| FaeI | CATG | 2 cut(s) | 39, 190 |
| FaiI | YATR | 3 cut(s) | 37, 147, 188 |
| FatI | CATG | 2 cut(s) | 35, 186 |
| Fnu4HI | GCNGC | 1 cut(s) | 41 |
| Fsp4HI | GCNGC | 1 cut(s) | 41 |
| FspAI | RTGCGCAY | 1 cut(s) | 191 |
| FspI | TGCGCA | 1 cut(s) | 191 |
| GlaI | GCGC | 1 cut(s) | 191 |
| GluI | GCNGC | 1 cut(s) | 41 |
| HaeIII | GGCC | 2 cut(s) | 66, 107 |
| HgaI | GACGC | 1 cut(s) | 55 |
| HhaI | GCGC | 1 cut(s) | 192 |
| Hin1II | CATG | 2 cut(s) | 39, 190 |
| Hin6I | GCGC | 1 cut(s) | 190 |
| HinP1I | GCGC | 1 cut(s) | 190 |
| HinfI | GANTC | 1 cut(s) | 31 |
| Hpy166II | GTNNAC | 1 cut(s) | 163 |
| Hpy188I | TCNGA | 2 cut(s) | 98, 178 |
| Hpy188III | TCNNGA | 1 cut(s) | 124 |
| Hpy8I | GTNNAC | 1 cut(s) | 163 |
| Hpy99I | CGWCG | 1 cut(s) | 65 |
| HpyAV | CCTTC | 1 cut(s) | 121 |
| HpyCH4IV | ACGT | 1 cut(s) | 60 |
| HpyCH4V | TGCA | 1 cut(s) | 20 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 46 |
| HpySE526I | ACGT | 1 cut(s) | 60 |
| Hsp92II | CATG | 2 cut(s) | 39, 190 |
| HspAI | GCGC | 1 cut(s) | 190 |
| Kzo9I | GATC | 2 cut(s) | 85, 178 |
| LmnI | GCTCC | 1 cut(s) | 179 |
| LpnPI | CCDG | 3 cut(s) | 61, 80, 89 |
| Lsp1109I | GCAGC | 1 cut(s) | 27 |
| MaeII | ACGT | 1 cut(s) | 60 |
| MalI | GATC | 2 cut(s) | 87, 180 |
| MboI | GATC | 2 cut(s) | 85, 178 |
| MhlI | GDGCHC | 1 cut(s) | 141 |
| MnlI | CCTC | 3 cut(s) | 16, 20, 112 |
| MseI | TTAA | 1 cut(s) | 114 |
| MspCI | CTTAAG | 1 cut(s) | 113 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 46 |
| NcoI | CCATGG | 1 cut(s) | 35 |
| NdeII | GATC | 2 cut(s) | 85, 178 |
| NlaIII | CATG | 2 cut(s) | 39, 190 |
| NmeAIII | GCCGAG | 1 cut(s) | 133 |
| NsbI | TGCGCA | 1 cut(s) | 191 |
| PfeI | GAWTC | 1 cut(s) | 31 |
| PkrI | GCNGC | 1 cut(s) | 42 |
| Ple19I | CGATCG | 1 cut(s) | 181 |
| PvuI | CGATCG | 1 cut(s) | 181 |
| SaqAI | TTAA | 1 cut(s) | 114 |
| SatI | GCNGC | 1 cut(s) | 41 |
| Sau3AI | GATC | 2 cut(s) | 85, 178 |
| SduI | GDGCHC | 1 cut(s) | 141 |
| SetI | ASST | 6 cut(s) | 12, 63, 96, 114, 123, 176 |
| SmlI | CTYRAG | 1 cut(s) | 113 |
| SmoI | CTYRAG | 1 cut(s) | 113 |
| SsiI | CCGC | 2 cut(s) | 43, 154 |
| StyI | CCWWGG | 1 cut(s) | 35 |
| TaiI | ACGT | 1 cut(s) | 63 |
| TaqI | TCGA | 2 cut(s) | 84, 123 |
| TfiI | GAWTC | 1 cut(s) | 31 |
| Tru1I | TTAA | 1 cut(s) | 114 |
| Tru9I | TTAA | 1 cut(s) | 114 |
| TseI | GCWGC | 1 cut(s) | 40 |
| Vha464I | CTTAAG | 1 cut(s) | 113 |