E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 769 |
| AccI | GTMKAC | 2 cut(s) | 666, 864 |
| AccIII | TCCGGA | 1 cut(s) | 33 |
| AciI | CCGC | 2 cut(s) | 682, 738 |
| AclWI | GGATC | 1 cut(s) | 50 |
| AcsI | RAATTY | 1 cut(s) | 355 |
| AdeI | CACNNNGTG | 1 cut(s) | 68 |
| AfaI | GTAC | 1 cut(s) | 194 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 443, 799 |
| AgsI | TTSAA | 8 cut(s) | 216, 335, 538, 575, 613, 638, 833, 880 |
| AluBI | AGCT | 6 cut(s) | 160, 394, 641, 649, 720, 883 |
| AluI | AGCT | 6 cut(s) | 160, 394, 641, 649, 720, 883 |
| Alw26I | GTCTC | 2 cut(s) | 419, 827 |
| AlwI | GGATC | 1 cut(s) | 50 |
| Aor13HI | TCCGGA | 1 cut(s) | 33 |
| AoxI | GGCC | 1 cut(s) | 705 |
| ApeKI | GCWGC | 3 cut(s) | 160, 323, 528 |
| ApoI | RAATTY | 1 cut(s) | 355 |
| AspS9I | GGNCC | 1 cut(s) | 36 |
| AsuHPI | GGTGA | 2 cut(s) | 704, 901 |
| AvaII | GGWCC | 1 cut(s) | 36 |
| BaeGI | GKGCMC | 1 cut(s) | 173 |
| BanI | GGYRCC | 1 cut(s) | 769 |
| BbsI | GAAGAC | 1 cut(s) | 78 |
| BbvI | GCAGC | 3 cut(s) | 147, 310, 540 |
| BccI | CCATC | 4 cut(s) | 130, 467, 549, 812 |
| BclI | TGATCA | 1 cut(s) | 502 |
| BcoDI | GTCTC | 2 cut(s) | 419, 827 |
| BfaI | CTAG | 3 cut(s) | 9, 144, 728 |
| BfmI | CTRYAG | 3 cut(s) | 385, 715, 721 |
| BisI | GCNGC | 3 cut(s) | 161, 324, 529 |
| BlsI | GCNGC | 3 cut(s) | 162, 325, 530 |
| Bme18I | GGWCC | 1 cut(s) | 36 |
| BmgT120I | GGNCC | 1 cut(s) | 36 |
| BmiI | GGNNCC | 2 cut(s) | 660, 771 |
| BmsI | GCATC | 4 cut(s) | 14, 94, 254, 579 |
| BpiI | GAAGAC | 1 cut(s) | 78 |
| BpmI | CTGGAG | 1 cut(s) | 185 |
| Bpu10I | CCTNAGC | 1 cut(s) | 884 |
| BsaAI | YACGTR | 1 cut(s) | 776 |
| BsaI | GGTCTC | 1 cut(s) | 827 |
| BsaJI | CCNNGG | 1 cut(s) | 248 |
| BsaWI | WCCGGW | 4 cut(s) | 33, 795, 867, 893 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 81, 111 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 443, 799 |
| Bse118I | RCCGGY | 1 cut(s) | 276 |
| Bse3DI | GCAATG | 1 cut(s) | 154 |
| BseAI | TCCGGA | 1 cut(s) | 33 |
| BseDI | CCNNGG | 1 cut(s) | 248 |
| BseGI | GGATG | 3 cut(s) | 30, 88, 594 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 443, 799 |
| BseMI | GCAATG | 1 cut(s) | 154 |
| BseMII | CTCAG | 3 cut(s) | 36, 422, 870 |
| BseSI | GKGCMC | 1 cut(s) | 173 |
| BseXI | GCAGC | 3 cut(s) | 147, 310, 540 |
| BshFI | GGCC | 1 cut(s) | 707 |
| BshNI | GGYRCC | 1 cut(s) | 769 |
| BsiSI | CCGG | 5 cut(s) | 34, 277, 796, 868, 894 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 443, 799 |
| BsmAI | GTCTC | 2 cut(s) | 419, 827 |
| BsmI | GAATGC | 2 cut(s) | 458, 674 |
| BsnI | GGCC | 1 cut(s) | 707 |
| Bso31I | GGTCTC | 1 cut(s) | 827 |
| Bsp1286I | GDGCHC | 1 cut(s) | 173 |
| Bsp13I | TCCGGA | 1 cut(s) | 33 |
| Bsp143I | GATC | 2 cut(s) | 55, 502 |
| BspACI | CCGC | 2 cut(s) | 682, 738 |
| BspANI | GGCC | 1 cut(s) | 707 |
| BspCNI | CTCAG | 3 cut(s) | 37, 421, 869 |
| BspEI | TCCGGA | 1 cut(s) | 33 |
| BspLI | GGNNCC | 2 cut(s) | 660, 771 |
| BspPI | GGATC | 1 cut(s) | 50 |
| BspT107I | GGYRCC | 1 cut(s) | 769 |
| BspTNI | GGTCTC | 1 cut(s) | 827 |
| BsrDI | GCAATG | 1 cut(s) | 154 |
| BsrFI | RCCGGY | 1 cut(s) | 276 |
| BssAI | RCCGGY | 1 cut(s) | 276 |
| BssECI | CCNNGG | 1 cut(s) | 248 |
| BssMI | GATC | 2 cut(s) | 55, 502 |
| BssT1I | CCWWGG | 1 cut(s) | 248 |
| Bst4CI | ACNGT | 4 cut(s) | 598, 725, 812, 915 |
| Bst6I | CTCTTC | 1 cut(s) | 854 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 769 |
| BstBAI | YACGTR | 1 cut(s) | 776 |
| BstC8I | GCNNGC | 3 cut(s) | 241, 278, 392 |
| BstDEI | CTNAG | 4 cut(s) | 45, 408, 856, 884 |
| BstEII | GGTNACC | 1 cut(s) | 889 |
| BstF5I | GGATG | 3 cut(s) | 30, 88, 594 |
| BstKTI | GATC | 2 cut(s) | 58, 505 |
| BstMAI | GTCTC | 2 cut(s) | 419, 827 |
| BstMBI | GATC | 2 cut(s) | 55, 502 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 177, 273, 769 |
| BstNSI | RCATGY | 2 cut(s) | 79, 520 |
| BstPI | GGTNACC | 1 cut(s) | 889 |
| BstSFI | CTRYAG | 3 cut(s) | 385, 715, 721 |
| BstSLI | GKGCMC | 1 cut(s) | 173 |
| BstV1I | GCAGC | 3 cut(s) | 147, 310, 540 |
| BstV2I | GAAGAC | 1 cut(s) | 78 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 359 |
| BsuRI | GGCC | 1 cut(s) | 707 |
| BtgZI | GCGATG | 1 cut(s) | 527 |
| BtsCI | GGATG | 3 cut(s) | 30, 88, 594 |
| BtsIMutI | CAGTG | 2 cut(s) | 215, 416 |
| Cac8I | GCNNGC | 3 cut(s) | 241, 278, 392 |
| Cfr10I | RCCGGY | 1 cut(s) | 276 |
| Cfr13I | GGNCC | 1 cut(s) | 36 |
| Csp6I | GTAC | 1 cut(s) | 193 |
| CviAII | CATG | 7 cut(s) | 76, 281, 342, 367, 401, 517, 688 |
| CviQI | GTAC | 1 cut(s) | 193 |
| DdeI | CTNAG | 4 cut(s) | 45, 408, 856, 884 |
| DpnI | GATC | 2 cut(s) | 57, 504 |
| DpnII | GATC | 2 cut(s) | 55, 502 |
| DraI | TTTAAA | 2 cut(s) | 114, 495 |
| DraIII | CACNNNGTG | 1 cut(s) | 68 |
| Eam1104I | CTCTTC | 1 cut(s) | 854 |
| EarI | CTCTTC | 1 cut(s) | 854 |
| Eco130I | CCWWGG | 1 cut(s) | 248 |
| Eco31I | GGTCTC | 1 cut(s) | 827 |
| Eco47I | GGWCC | 1 cut(s) | 36 |
| Eco91I | GGTNACC | 1 cut(s) | 889 |
| EcoO65I | GGTNACC | 1 cut(s) | 889 |
| EcoT14I | CCWWGG | 1 cut(s) | 248 |
| ErhI | CCWWGG | 1 cut(s) | 248 |
| FaeI | CATG | 7 cut(s) | 79, 284, 345, 370, 404, 520, 691 |
| FatI | CATG | 7 cut(s) | 75, 280, 341, 366, 400, 516, 687 |
| FauI | CCCGC | 1 cut(s) | 731 |
| FbaI | TGATCA | 1 cut(s) | 502 |
| FblI | GTMKAC | 2 cut(s) | 666, 864 |
| Fnu4HI | GCNGC | 3 cut(s) | 161, 324, 529 |
| FokI | GGATG | 3 cut(s) | 17, 95, 601 |
| Fsp4HI | GCNGC | 3 cut(s) | 161, 324, 529 |
| FspBI | CTAG | 3 cut(s) | 9, 144, 728 |
| GluI | GCNGC | 3 cut(s) | 161, 324, 529 |
| GsuI | CTGGAG | 1 cut(s) | 185 |
| HaeIII | GGCC | 1 cut(s) | 707 |
| HapII | CCGG | 5 cut(s) | 34, 277, 796, 868, 894 |
| Hin1II | CATG | 7 cut(s) | 79, 284, 345, 370, 404, 520, 691 |
| HincII | GTYRAC | 1 cut(s) | 514 |
| HindII | GTYRAC | 1 cut(s) | 514 |
| HindIII | AAGCTT | 2 cut(s) | 639, 881 |
| HinfI | GANTC | 1 cut(s) | 404 |
| HpaII | CCGG | 5 cut(s) | 34, 277, 796, 868, 894 |
| HphI | GGTGA | 2 cut(s) | 704, 901 |
| Hpy166II | GTNNAC | 4 cut(s) | 256, 514, 667, 865 |
| Hpy188I | TCNGA | 3 cut(s) | 46, 451, 653 |
| Hpy188III | TCNNGA | 4 cut(s) | 34, 332, 728, 806 |
| Hpy8I | GTNNAC | 4 cut(s) | 256, 514, 667, 865 |
| HpyAV | CCTTC | 5 cut(s) | 310, 793, 865, 891, 901 |
| HpyCH4III | ACNGT | 4 cut(s) | 598, 725, 812, 915 |
| HpyCH4IV | ACGT | 1 cut(s) | 775 |
| HpyCH4V | TGCA | 5 cut(s) | 243, 308, 366, 456, 520 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 177, 273, 769 |
| HpyF3I | CTNAG | 4 cut(s) | 45, 408, 856, 884 |
| HpySE526I | ACGT | 1 cut(s) | 775 |
| Hsp92II | CATG | 7 cut(s) | 79, 284, 345, 370, 404, 520, 691 |
| Kpn2I | TCCGGA | 1 cut(s) | 33 |
| KroI | GCCGGC | 1 cut(s) | 276 |
| KroNI | GCCGGC | 1 cut(s) | 278 |
| Ksp22I | TGATCA | 1 cut(s) | 502 |
| Kzo9I | GATC | 2 cut(s) | 55, 502 |
| LmnI | GCTCC | 1 cut(s) | 664 |
| Lsp1109I | GCAGC | 3 cut(s) | 147, 310, 540 |
| LweI | GCATC | 4 cut(s) | 14, 94, 254, 579 |
| MaeI | CTAG | 3 cut(s) | 9, 144, 728 |
| MaeII | ACGT | 1 cut(s) | 775 |
| MaeIII | GTNAC | 3 cut(s) | 221, 806, 889 |
| MalI | GATC | 2 cut(s) | 57, 504 |
| MboI | GATC | 2 cut(s) | 55, 502 |
| MboII | GAAGA | 4 cut(s) | 83, 763, 841, 844 |
| MhlI | GDGCHC | 1 cut(s) | 173 |
| MluCI | AATT | 4 cut(s) | 51, 201, 355, 875 |
| MmeI | TCCRAC | 2 cut(s) | 67, 445 |
| MnlI | CCTC | 4 cut(s) | 40, 69, 697, 741 |
| MroI | TCCGGA | 1 cut(s) | 33 |
| MroNI | GCCGGC | 1 cut(s) | 276 |
| MseI | TTAA | 3 cut(s) | 113, 182, 494 |
| MslI | CAYNNNNRTG | 1 cut(s) | 546 |
| MspI | CCGG | 5 cut(s) | 34, 277, 796, 868, 894 |
| Mva1269I | GAATGC | 2 cut(s) | 458, 674 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 177, 273, 769 |
| NaeI | GCCGGC | 1 cut(s) | 278 |
| NdeII | GATC | 2 cut(s) | 55, 502 |
| NgoMIV | GCCGGC | 1 cut(s) | 276 |
| NlaIII | CATG | 7 cut(s) | 79, 284, 345, 370, 404, 520, 691 |
| NlaIV | GGNNCC | 2 cut(s) | 660, 771 |
| NmuCI | GTSAC | 3 cut(s) | 221, 806, 889 |
| NspI | RCATGY | 2 cut(s) | 79, 520 |
| PctI | GAATGC | 2 cut(s) | 458, 674 |
| PdiI | GCCGGC | 1 cut(s) | 278 |
| PfeI | GAWTC | 1 cut(s) | 404 |
| PkrI | GCNGC | 3 cut(s) | 162, 325, 530 |
| Ppu21I | YACGTR | 1 cut(s) | 776 |
| PspEI | GGTNACC | 1 cut(s) | 889 |
| PspN4I | GGNNCC | 2 cut(s) | 660, 771 |
| PspPI | GGNCC | 1 cut(s) | 36 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 704, 736 |
| RsaI | GTAC | 1 cut(s) | 194 |
| RsaNI | GTAC | 1 cut(s) | 193 |
| RseI | CAYNNNNRTG | 1 cut(s) | 546 |
| SaqAI | TTAA | 3 cut(s) | 113, 182, 494 |
| SatI | GCNGC | 3 cut(s) | 161, 324, 529 |
| Sau3AI | GATC | 2 cut(s) | 55, 502 |
| Sau96I | GGNCC | 1 cut(s) | 36 |
| SduI | GDGCHC | 1 cut(s) | 173 |
| SfaNI | GCATC | 4 cut(s) | 14, 94, 254, 579 |
| SfcI | CTRYAG | 3 cut(s) | 385, 715, 721 |
| SinI | GGWCC | 1 cut(s) | 36 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 546 |
| Sse9I | AATT | 4 cut(s) | 51, 201, 355, 875 |
| SsiI | CCGC | 2 cut(s) | 682, 738 |
| SspMI | CTAG | 3 cut(s) | 9, 144, 728 |
| StyI | CCWWGG | 1 cut(s) | 248 |
| TaaI | ACNGT | 4 cut(s) | 598, 725, 812, 915 |
| TaiI | ACGT | 1 cut(s) | 778 |
| TaqI | TCGA | 1 cut(s) | 99 |
| TasI | AATT | 4 cut(s) | 51, 201, 355, 875 |
| TfiI | GAWTC | 1 cut(s) | 404 |
| Tru1I | TTAA | 3 cut(s) | 113, 182, 494 |
| Tru9I | TTAA | 3 cut(s) | 113, 182, 494 |
| TscAI | CASTG | 2 cut(s) | 215, 416 |
| TseFI | GTSAC | 3 cut(s) | 221, 806, 889 |
| TseI | GCWGC | 3 cut(s) | 160, 323, 528 |
| Tsp45I | GTSAC | 3 cut(s) | 221, 806, 889 |
| TspDTI | ATGAA | 4 cut(s) | 194, 297, 417, 676 |
| TspGWI | ACGGA | 1 cut(s) | 143 |
| TspRI | CASTG | 2 cut(s) | 215, 416 |
| VpaK11BI | GGWCC | 1 cut(s) | 36 |
| XapI | RAATTY | 1 cut(s) | 355 |
| XbaI | TCTAGA | 1 cut(s) | 727 |
| XceI | RCATGY | 2 cut(s) | 79, 520 |
| XmiI | GTMKAC | 2 cut(s) | 666, 864 |
| XspI | CTAG | 3 cut(s) | 9, 144, 728 |