E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 402 |
| AccII | CGCG | 1 cut(s) | 522 |
| AciI | CCGC | 3 cut(s) | 16, 477, 629 |
| AclWI | GGATC | 2 cut(s) | 180, 271 |
| AcoI | YGGCCR | 2 cut(s) | 54, 349 |
| AcsI | RAATTY | 2 cut(s) | 192, 504 |
| AcuI | CTGAAG | 1 cut(s) | 635 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 535 |
| AgsI | TTSAA | 2 cut(s) | 274, 311 |
| AjnI | CCWGG | 2 cut(s) | 7, 168 |
| AluBI | AGCT | 5 cut(s) | 124, 232, 490, 613, 619 |
| AluI | AGCT | 5 cut(s) | 124, 232, 490, 613, 619 |
| Alw26I | GTCTC | 2 cut(s) | 9, 467 |
| AlwI | GGATC | 2 cut(s) | 180, 271 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 398, 613 |
| AoxI | GGCC | 6 cut(s) | 13, 54, 349, 408, 441, 631 |
| ApeKI | GCWGC | 1 cut(s) | 68 |
| ApoI | RAATTY | 2 cut(s) | 192, 504 |
| AspS9I | GGNCC | 1 cut(s) | 631 |
| AsuHPI | GGTGA | 1 cut(s) | 440 |
| BbvI | GCAGC | 1 cut(s) | 80 |
| BccI | CCATC | 3 cut(s) | 254, 341, 548 |
| BceAI | ACGGC | 2 cut(s) | 336, 618 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 47, 81 |
| BciT130I | CCWGG | 2 cut(s) | 9, 170 |
| BclI | TGATCA | 1 cut(s) | 561 |
| BcoDI | GTCTC | 2 cut(s) | 9, 467 |
| BfaI | CTAG | 1 cut(s) | 87 |
| BfmI | CTRYAG | 1 cut(s) | 223 |
| BisI | GCNGC | 2 cut(s) | 16, 69 |
| BlsI | GCNGC | 2 cut(s) | 17, 70 |
| Bme1390I | CCNGG | 2 cut(s) | 9, 170 |
| BmgT120I | GGNCC | 1 cut(s) | 631 |
| BmrFI | CCNGG | 2 cut(s) | 9, 170 |
| BmsI | GCATC | 1 cut(s) | 55 |
| BpmI | CTGGAG | 2 cut(s) | 30, 170 |
| BsaAI | YACGTR | 1 cut(s) | 321 |
| BsaJI | CCNNGG | 2 cut(s) | 168, 509 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 535 |
| Bse1I | ACTGG | 1 cut(s) | 153 |
| BseBI | CCWGG | 2 cut(s) | 9, 170 |
| BseDI | CCNNGG | 2 cut(s) | 168, 509 |
| BseGI | GGATG | 2 cut(s) | 34, 330 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 535 |
| BseMII | CTCAG | 1 cut(s) | 606 |
| BseNI | ACTGG | 1 cut(s) | 153 |
| BseRI | GAGGAG | 1 cut(s) | 526 |
| BseXI | GCAGC | 1 cut(s) | 80 |
| BsgI | GTGCAG | 1 cut(s) | 113 |
| Bsh1236I | CGCG | 1 cut(s) | 522 |
| BshFI | GGCC | 6 cut(s) | 15, 56, 351, 410, 443, 633 |
| BslFI | GGGAC | 1 cut(s) | 151 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 535 |
| BsmAI | GTCTC | 2 cut(s) | 9, 467 |
| BsmFI | GGGAC | 1 cut(s) | 151 |
| BsmI | GAATGC | 1 cut(s) | 377 |
| BsnI | GGCC | 6 cut(s) | 15, 56, 351, 410, 443, 633 |
| Bsp143I | GATC | 3 cut(s) | 172, 263, 561 |
| Bsp68I | TCGCGA | 1 cut(s) | 522 |
| BspACI | CCGC | 3 cut(s) | 16, 477, 629 |
| BspANI | GGCC | 6 cut(s) | 15, 56, 351, 410, 443, 633 |
| BspCNI | CTCAG | 1 cut(s) | 605 |
| BspFNI | CGCG | 1 cut(s) | 522 |
| BspPI | GGATC | 2 cut(s) | 180, 271 |
| BsrI | ACTGG | 1 cut(s) | 153 |
| BssECI | CCNNGG | 2 cut(s) | 168, 509 |
| BssMI | GATC | 3 cut(s) | 172, 263, 561 |
| BssNAI | GTATAC | 1 cut(s) | 403 |
| Bst1107I | GTATAC | 1 cut(s) | 403 |
| Bst2UI | CCWGG | 2 cut(s) | 9, 170 |
| Bst4CI | ACNGT | 4 cut(s) | 101, 344, 461, 548 |
| BstBAI | YACGTR | 1 cut(s) | 321 |
| BstC8I | GCNNGC | 3 cut(s) | 230, 365, 631 |
| BstDEI | CTNAG | 2 cut(s) | 416, 592 |
| BstF5I | GGATG | 2 cut(s) | 34, 330 |
| BstFNI | CGCG | 1 cut(s) | 522 |
| BstKTI | GATC | 3 cut(s) | 175, 266, 564 |
| BstMAI | GTCTC | 2 cut(s) | 9, 467 |
| BstMBI | GATC | 3 cut(s) | 172, 263, 561 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 21, 496 |
| BstNI | CCWGG | 2 cut(s) | 9, 170 |
| BstNSI | RCATGY | 1 cut(s) | 256 |
| BstSCI | CCNGG | 2 cut(s) | 7, 168 |
| BstSFI | CTRYAG | 1 cut(s) | 223 |
| BstUI | CGCG | 1 cut(s) | 522 |
| BstV1I | GCAGC | 1 cut(s) | 80 |
| BstX2I | RGATCY | 2 cut(s) | 172, 263 |
| BstYI | RGATCY | 2 cut(s) | 172, 263 |
| BstZ17I | GTATAC | 1 cut(s) | 403 |
| BsuRI | GGCC | 6 cut(s) | 15, 56, 351, 410, 443, 633 |
| BtsCI | GGATG | 2 cut(s) | 34, 330 |
| BtsIMutI | CAGTG | 2 cut(s) | 146, 649 |
| BtuMI | TCGCGA | 1 cut(s) | 522 |
| Cac8I | GCNNGC | 3 cut(s) | 230, 365, 631 |
| CaiI | CAGNNNCTG | 2 cut(s) | 398, 613 |
| Cfr13I | GGNCC | 1 cut(s) | 631 |
| CviAII | CATG | 6 cut(s) | 239, 253, 257, 406, 424, 495 |
| DdeI | CTNAG | 2 cut(s) | 416, 592 |
| DpnI | GATC | 3 cut(s) | 174, 265, 563 |
| DpnII | GATC | 3 cut(s) | 172, 263, 561 |
| EaeI | YGGCCR | 2 cut(s) | 54, 349 |
| Eco32I | GATATC | 1 cut(s) | 188 |
| Eco57I | CTGAAG | 1 cut(s) | 635 |
| EcoRI | GAATTC | 1 cut(s) | 504 |
| EcoRII | CCWGG | 2 cut(s) | 7, 168 |
| EcoRV | GATATC | 1 cut(s) | 188 |
| EcoT22I | ATGCAT | 1 cut(s) | 258 |
| FaeI | CATG | 6 cut(s) | 242, 256, 260, 409, 427, 498 |
| FaqI | GGGAC | 1 cut(s) | 151 |
| FatI | CATG | 6 cut(s) | 238, 252, 256, 405, 423, 494 |
| FauI | CCCGC | 1 cut(s) | 622 |
| FbaI | TGATCA | 1 cut(s) | 561 |
| FblI | GTMKAC | 1 cut(s) | 402 |
| Fnu4HI | GCNGC | 2 cut(s) | 16, 69 |
| FokI | GGATG | 2 cut(s) | 41, 337 |
| Fsp4HI | GCNGC | 2 cut(s) | 16, 69 |
| FspBI | CTAG | 1 cut(s) | 87 |
| GluI | GCNGC | 2 cut(s) | 16, 69 |
| GsuI | CTGGAG | 2 cut(s) | 30, 170 |
| HaeIII | GGCC | 6 cut(s) | 15, 56, 351, 410, 443, 633 |
| Hin1II | CATG | 6 cut(s) | 242, 256, 260, 409, 427, 498 |
| HincII | GTYRAC | 2 cut(s) | 250, 330 |
| HindII | GTYRAC | 2 cut(s) | 250, 330 |
| HinfI | GANTC | 3 cut(s) | 59, 164, 420 |
| HpaI | GTTAAC | 1 cut(s) | 330 |
| HphI | GGTGA | 1 cut(s) | 440 |
| Hpy166II | GTNNAC | 3 cut(s) | 250, 330, 403 |
| Hpy188I | TCNGA | 3 cut(s) | 64, 510, 570 |
| Hpy188III | TCNNGA | 4 cut(s) | 464, 521, 542, 551 |
| Hpy8I | GTNNAC | 3 cut(s) | 250, 330, 403 |
| HpyAV | CCTTC | 1 cut(s) | 421 |
| HpyCH4III | ACNGT | 4 cut(s) | 101, 344, 461, 548 |
| HpyCH4IV | ACGT | 1 cut(s) | 320 |
| HpyCH4V | TGCA | 4 cut(s) | 68, 94, 256, 367 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 21, 496 |
| HpyF3I | CTNAG | 2 cut(s) | 416, 592 |
| HpySE526I | ACGT | 1 cut(s) | 320 |
| Hsp92II | CATG | 6 cut(s) | 242, 256, 260, 409, 427, 498 |
| Ksp22I | TGATCA | 1 cut(s) | 561 |
| KspAI | GTTAAC | 1 cut(s) | 330 |
| Kzo9I | GATC | 3 cut(s) | 172, 263, 561 |
| LmnI | GCTCC | 1 cut(s) | 610 |
| LpnPI | CCDG | 9 cut(s) | 21, 91, 134, 155, 182, 219, 411, 579, 593 |
| Lsp1109I | GCAGC | 1 cut(s) | 80 |
| LweI | GCATC | 1 cut(s) | 55 |
| MaeI | CTAG | 1 cut(s) | 87 |
| MaeII | ACGT | 1 cut(s) | 320 |
| MaeIII | GTNAC | 1 cut(s) | 542 |
| MalI | GATC | 3 cut(s) | 174, 265, 563 |
| MboI | GATC | 3 cut(s) | 172, 263, 561 |
| MboII | GAAGA | 2 cut(s) | 167, 580 |
| MflI | RGATCY | 2 cut(s) | 172, 263 |
| MluCI | AATT | 3 cut(s) | 192, 449, 504 |
| MlyI | GAGTC | 1 cut(s) | 173 |
| MnlI | CCTC | 6 cut(s) | 5, 19, 367, 433, 504, 564 |
| Mph1103I | ATGCAT | 1 cut(s) | 258 |
| MseI | TTAA | 3 cut(s) | 288, 329, 339 |
| MslI | CAYNNNNRTG | 1 cut(s) | 282 |
| MspR9I | CCNGG | 2 cut(s) | 9, 170 |
| Mva1269I | GAATGC | 1 cut(s) | 377 |
| MvaI | CCWGG | 2 cut(s) | 9, 170 |
| MvnI | CGCG | 1 cut(s) | 522 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 21, 496 |
| NdeII | GATC | 3 cut(s) | 172, 263, 561 |
| NlaIII | CATG | 6 cut(s) | 242, 256, 260, 409, 427, 498 |
| NmuCI | GTSAC | 1 cut(s) | 542 |
| NruI | TCGCGA | 1 cut(s) | 522 |
| NsiI | ATGCAT | 1 cut(s) | 258 |
| NspI | RCATGY | 1 cut(s) | 256 |
| PctI | GAATGC | 1 cut(s) | 377 |
| PfeI | GAWTC | 2 cut(s) | 59, 420 |
| PfoI | TCCNGGA | 1 cut(s) | 7 |
| PkrI | GCNGC | 2 cut(s) | 17, 70 |
| PleI | GAGTC | 1 cut(s) | 172 |
| PpsI | GAGTC | 1 cut(s) | 172 |
| Ppu21I | YACGTR | 1 cut(s) | 321 |
| Psp6I | CCWGG | 2 cut(s) | 7, 168 |
| PspGI | CCWGG | 2 cut(s) | 7, 168 |
| PspPI | GGNCC | 1 cut(s) | 631 |
| PstNI | CAGNNNCTG | 2 cut(s) | 398, 613 |
| PsuI | RGATCY | 2 cut(s) | 172, 263 |
| RruI | TCGCGA | 1 cut(s) | 522 |
| RseI | CAYNNNNRTG | 1 cut(s) | 282 |
| SaqAI | TTAA | 3 cut(s) | 288, 329, 339 |
| SatI | GCNGC | 2 cut(s) | 16, 69 |
| Sau3AI | GATC | 3 cut(s) | 172, 263, 561 |
| Sau96I | GGNCC | 1 cut(s) | 631 |
| SchI | GAGTC | 1 cut(s) | 173 |
| ScrFI | CCNGG | 2 cut(s) | 9, 170 |
| SfaNI | GCATC | 1 cut(s) | 55 |
| SfcI | CTRYAG | 1 cut(s) | 223 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 282 |
| Sse9I | AATT | 3 cut(s) | 192, 449, 504 |
| SsiI | CCGC | 3 cut(s) | 16, 477, 629 |
| SspMI | CTAG | 1 cut(s) | 87 |
| StyD4I | CCNGG | 2 cut(s) | 7, 168 |
| TaaI | ACNGT | 4 cut(s) | 101, 344, 461, 548 |
| TaiI | ACGT | 1 cut(s) | 323 |
| TaqI | TCGA | 1 cut(s) | 465 |
| TasI | AATT | 3 cut(s) | 192, 449, 504 |
| TauI | GCSGC | 1 cut(s) | 18 |
| TfiI | GAWTC | 2 cut(s) | 59, 420 |
| Tru1I | TTAA | 3 cut(s) | 288, 329, 339 |
| Tru9I | TTAA | 3 cut(s) | 288, 329, 339 |
| TscAI | CASTG | 1 cut(s) | 153 |
| TseFI | GTSAC | 1 cut(s) | 542 |
| TseI | GCWGC | 1 cut(s) | 68 |
| Tsp45I | GTSAC | 1 cut(s) | 542 |
| TspDTI | ATGAA | 1 cut(s) | 412 |
| TspRI | CASTG | 1 cut(s) | 153 |
| XapI | RAATTY | 2 cut(s) | 192, 504 |
| XceI | RCATGY | 1 cut(s) | 256 |
| XmiI | GTMKAC | 1 cut(s) | 402 |
| XspI | CTAG | 1 cut(s) | 87 |
| Zsp2I | ATGCAT | 1 cut(s) | 258 |