E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 600 |
| AccII | CGCG | 1 cut(s) | 720 |
| AciI | CCGC | 2 cut(s) | 211, 718 |
| AclWI | GGATC | 1 cut(s) | 388 |
| AcoI | YGGCCR | 2 cut(s) | 154, 829 |
| AcsI | RAATTY | 2 cut(s) | 647, 702 |
| AcuI | CTGAAG | 2 cut(s) | 350, 833 |
| AfaI | GTAC | 2 cut(s) | 128, 466 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 44, 49, 50, 377 |
| AflII | CTTAAG | 1 cut(s) | 428 |
| AflIII | ACRYGT | 1 cut(s) | 159 |
| AgeI | ACCGGT | 1 cut(s) | 398 |
| AgsI | TTSAA | 3 cut(s) | 75, 472, 509 |
| AjnI | CCWGG | 3 cut(s) | 43, 707, 747 |
| AluBI | AGCT | 3 cut(s) | 220, 328, 654 |
| AluI | AGCT | 3 cut(s) | 220, 328, 654 |
| Alw21I | GWGCWC | 2 cut(s) | 28, 392 |
| Alw44I | GTGCAC | 1 cut(s) | 24 |
| AlwI | GGATC | 1 cut(s) | 388 |
| AoxI | GGCC | 2 cut(s) | 154, 829 |
| ApaLI | GTGCAC | 1 cut(s) | 24 |
| ApoI | RAATTY | 2 cut(s) | 647, 702 |
| AsiGI | ACCGGT | 1 cut(s) | 398 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 647 |
| AspLEI | GCGC | 1 cut(s) | 781 |
| AsuHPI | GGTGA | 1 cut(s) | 413 |
| BaeGI | GKGCMC | 2 cut(s) | 28, 107 |
| BalI | TGGCCA | 1 cut(s) | 156 |
| BbsI | GAAGAC | 1 cut(s) | 556 |
| Bbv12I | GWGCWC | 2 cut(s) | 28, 392 |
| BccI | CCATC | 3 cut(s) | 452, 577, 746 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 329, 363 |
| BciT130I | CCWGG | 3 cut(s) | 45, 709, 749 |
| BciVI | GTATCC | 1 cut(s) | 725 |
| BclI | TGATCA | 1 cut(s) | 436 |
| BfmI | CTRYAG | 2 cut(s) | 319, 655 |
| BfoI | RGCGCY | 1 cut(s) | 782 |
| BfrI | CTTAAG | 1 cut(s) | 428 |
| BfuI | GTATCC | 1 cut(s) | 725 |
| BisI | GCNGC | 1 cut(s) | 211 |
| BlsI | GCNGC | 1 cut(s) | 212 |
| Bme1390I | CCNGG | 3 cut(s) | 45, 709, 749 |
| BmiI | GGNNCC | 1 cut(s) | 594 |
| BmrFI | CCNGG | 3 cut(s) | 45, 709, 749 |
| BmsI | GCATC | 1 cut(s) | 513 |
| BpiI | GAAGAC | 1 cut(s) | 556 |
| BpmI | CTGGAG | 2 cut(s) | 119, 579 |
| BsaJI | CCNNGG | 2 cut(s) | 43, 707 |
| BsaWI | WCCGGW | 1 cut(s) | 398 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 44, 49, 50, 377 |
| Bse118I | RCCGGY | 1 cut(s) | 398 |
| Bse1I | ACTGG | 3 cut(s) | 102, 596, 832 |
| BseBI | CCWGG | 3 cut(s) | 45, 709, 749 |
| BseDI | CCNNGG | 2 cut(s) | 43, 707 |
| BseGI | GGATG | 3 cut(s) | 439, 528, 757 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 44, 49, 50, 377 |
| BseMII | CTCAG | 2 cut(s) | 375, 627 |
| BseNI | ACTGG | 3 cut(s) | 102, 596, 832 |
| BseSI | GKGCMC | 2 cut(s) | 28, 107 |
| BsgI | GTGCAG | 2 cut(s) | 97, 209 |
| Bsh1236I | CGCG | 1 cut(s) | 720 |
| BshFI | GGCC | 2 cut(s) | 156, 831 |
| BshTI | ACCGGT | 1 cut(s) | 398 |
| BsiHKAI | GWGCWC | 2 cut(s) | 28, 392 |
| BsiSI | CCGG | 2 cut(s) | 399, 832 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 44, 49, 50, 377 |
| BsnI | GGCC | 2 cut(s) | 156, 831 |
| Bsp1286I | GDGCHC | 3 cut(s) | 28, 107, 392 |
| Bsp143I | GATC | 2 cut(s) | 380, 436 |
| BspACI | CCGC | 2 cut(s) | 211, 718 |
| BspANI | GGCC | 2 cut(s) | 156, 831 |
| BspCNI | CTCAG | 2 cut(s) | 376, 628 |
| BspFNI | CGCG | 1 cut(s) | 720 |
| BspHI | TCATGA | 1 cut(s) | 301 |
| BspLI | GGNNCC | 1 cut(s) | 594 |
| BspPI | GGATC | 1 cut(s) | 388 |
| BspTI | CTTAAG | 1 cut(s) | 428 |
| BsrFI | RCCGGY | 1 cut(s) | 398 |
| BsrI | ACTGG | 3 cut(s) | 102, 596, 832 |
| BssAI | RCCGGY | 1 cut(s) | 398 |
| BssECI | CCNNGG | 2 cut(s) | 43, 707 |
| BssMI | GATC | 2 cut(s) | 380, 436 |
| Bst2UI | CCWGG | 3 cut(s) | 45, 709, 749 |
| Bst4CI | ACNGT | 3 cut(s) | 532, 555, 725 |
| BstAFI | CTTAAG | 1 cut(s) | 428 |
| BstC8I | GCNNGC | 1 cut(s) | 326 |
| BstDEI | CTNAG | 2 cut(s) | 384, 636 |
| BstF5I | GGATG | 3 cut(s) | 439, 528, 757 |
| BstFNI | CGCG | 1 cut(s) | 720 |
| BstH2I | RGCGCY | 1 cut(s) | 782 |
| BstHHI | GCGC | 1 cut(s) | 781 |
| BstKTI | GATC | 2 cut(s) | 383, 439 |
| BstMBI | GATC | 2 cut(s) | 380, 436 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 111, 207 |
| BstNI | CCWGG | 3 cut(s) | 45, 709, 749 |
| BstNSI | RCATGY | 1 cut(s) | 454 |
| BstSCI | CCNGG | 3 cut(s) | 43, 707, 747 |
| BstSFI | CTRYAG | 2 cut(s) | 319, 655 |
| BstSLI | GKGCMC | 2 cut(s) | 28, 107 |
| BstUI | CGCG | 1 cut(s) | 720 |
| BstV2I | GAAGAC | 1 cut(s) | 556 |
| BstX2I | RGATCY | 1 cut(s) | 380 |
| BstYI | RGATCY | 1 cut(s) | 380 |
| BsuI | GTATCC | 1 cut(s) | 725 |
| BsuRI | GGCC | 2 cut(s) | 156, 831 |
| BtgZI | GCGATG | 1 cut(s) | 642 |
| BtsCI | GGATG | 3 cut(s) | 439, 528, 757 |
| BtsIMutI | CAGTG | 1 cut(s) | 149 |
| Cac8I | GCNNGC | 1 cut(s) | 326 |
| CciI | TCATGA | 1 cut(s) | 301 |
| CfoI | GCGC | 1 cut(s) | 781 |
| Cfr10I | RCCGGY | 1 cut(s) | 398 |
| Csp6I | GTAC | 2 cut(s) | 127, 465 |
| CspAI | ACCGGT | 1 cut(s) | 398 |
| CviQI | GTAC | 2 cut(s) | 127, 465 |
| DdeI | CTNAG | 2 cut(s) | 384, 636 |
| DpnI | GATC | 2 cut(s) | 382, 438 |
| DpnII | GATC | 2 cut(s) | 380, 436 |
| EaeI | YGGCCR | 2 cut(s) | 154, 829 |
| Eco32I | GATATC | 1 cut(s) | 406 |
| Eco57I | CTGAAG | 2 cut(s) | 350, 833 |
| EcoRI | GAATTC | 1 cut(s) | 702 |
| EcoRII | CCWGG | 3 cut(s) | 43, 707, 747 |
| EcoRV | GATATC | 1 cut(s) | 406 |
| EcoT22I | ATGCAT | 2 cut(s) | 281, 456 |
| FbaI | TGATCA | 1 cut(s) | 436 |
| FblI | GTMKAC | 1 cut(s) | 600 |
| Fnu4HI | GCNGC | 1 cut(s) | 211 |
| FokI | GGATG | 3 cut(s) | 446, 535, 764 |
| Fsp4HI | GCNGC | 1 cut(s) | 211 |
| GlaI | GCGC | 1 cut(s) | 780 |
| GluI | GCNGC | 1 cut(s) | 211 |
| GsuI | CTGGAG | 2 cut(s) | 119, 579 |
| HaeII | RGCGCY | 1 cut(s) | 782 |
| HaeIII | GGCC | 2 cut(s) | 156, 831 |
| HapII | CCGG | 2 cut(s) | 399, 832 |
| HhaI | GCGC | 1 cut(s) | 781 |
| Hin6I | GCGC | 1 cut(s) | 779 |
| HinP1I | GCGC | 1 cut(s) | 779 |
| HincII | GTYRAC | 1 cut(s) | 448 |
| HindII | GTYRAC | 1 cut(s) | 448 |
| HinfI | GANTC | 3 cut(s) | 260, 618, 756 |
| HpaII | CCGG | 2 cut(s) | 399, 832 |
| HphI | GGTGA | 1 cut(s) | 413 |
| Hpy166II | GTNNAC | 4 cut(s) | 26, 448, 465, 601 |
| Hpy188I | TCNGA | 3 cut(s) | 13, 172, 385 |
| Hpy188III | TCNNGA | 3 cut(s) | 302, 580, 740 |
| Hpy8I | GTNNAC | 4 cut(s) | 26, 448, 465, 601 |
| HpyAV | CCTTC | 2 cut(s) | 244, 478 |
| HpyCH4III | ACNGT | 3 cut(s) | 532, 555, 725 |
| HpyCH4IV | ACGT | 1 cut(s) | 161 |
| HpyCH4V | TGCA | 9 cut(s) | 26, 88, 114, 177, 190, 279, 454, 515, 565 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 111, 207 |
| HpyF3I | CTNAG | 2 cut(s) | 384, 636 |
| HpySE526I | ACGT | 1 cut(s) | 161 |
| HspAI | GCGC | 1 cut(s) | 779 |
| Ksp22I | TGATCA | 1 cut(s) | 436 |
| Kzo9I | GATC | 2 cut(s) | 380, 436 |
| LmnI | GCTCC | 1 cut(s) | 598 |
| LweI | GCATC | 1 cut(s) | 513 |
| MaeII | ACGT | 1 cut(s) | 161 |
| MaeIII | GTNAC | 3 cut(s) | 93, 394, 740 |
| MalI | GATC | 2 cut(s) | 382, 438 |
| MboI | GATC | 2 cut(s) | 380, 436 |
| MboII | GAAGA | 2 cut(s) | 561, 655 |
| MfeI | CAATTG | 1 cut(s) | 364 |
| MflI | RGATCY | 1 cut(s) | 380 |
| MhlI | GDGCHC | 3 cut(s) | 28, 107, 392 |
| MlsI | TGGCCA | 1 cut(s) | 156 |
| MluCI | AATT | 5 cut(s) | 193, 364, 538, 647, 702 |
| MluNI | TGGCCA | 1 cut(s) | 156 |
| MlyI | GAGTC | 1 cut(s) | 750 |
| MnlI | CCTC | 5 cut(s) | 141, 565, 631, 658, 813 |
| Mox20I | TGGCCA | 1 cut(s) | 156 |
| Mph1103I | ATGCAT | 2 cut(s) | 281, 456 |
| MroXI | GAANNNNTTC | 1 cut(s) | 647 |
| MscI | TGGCCA | 1 cut(s) | 156 |
| MseI | TTAA | 6 cut(s) | 30, 420, 429, 486, 537, 689 |
| MslI | CAYNNNNRTG | 1 cut(s) | 480 |
| Msp20I | TGGCCA | 1 cut(s) | 156 |
| MspCI | CTTAAG | 1 cut(s) | 428 |
| MspI | CCGG | 2 cut(s) | 399, 832 |
| MspR9I | CCNGG | 3 cut(s) | 45, 709, 749 |
| MunI | CAATTG | 1 cut(s) | 364 |
| MvaI | CCWGG | 3 cut(s) | 45, 709, 749 |
| MvnI | CGCG | 1 cut(s) | 720 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 111, 207 |
| NdeII | GATC | 2 cut(s) | 380, 436 |
| NlaIV | GGNNCC | 1 cut(s) | 594 |
| NmuCI | GTSAC | 1 cut(s) | 740 |
| NsiI | ATGCAT | 2 cut(s) | 281, 456 |
| NspI | RCATGY | 1 cut(s) | 454 |
| PagI | TCATGA | 1 cut(s) | 301 |
| PdmI | GAANNNNTTC | 1 cut(s) | 647 |
| PfeI | GAWTC | 2 cut(s) | 260, 618 |
| PinAI | ACCGGT | 1 cut(s) | 398 |
| PkrI | GCNGC | 1 cut(s) | 212 |
| PleI | GAGTC | 1 cut(s) | 750 |
| PpsI | GAGTC | 1 cut(s) | 750 |
| Psp6I | CCWGG | 3 cut(s) | 43, 707, 747 |
| PspGI | CCWGG | 3 cut(s) | 43, 707, 747 |
| PspN4I | GGNNCC | 1 cut(s) | 594 |
| PsuI | RGATCY | 1 cut(s) | 380 |
| RsaI | GTAC | 2 cut(s) | 128, 466 |
| RsaNI | GTAC | 2 cut(s) | 127, 465 |
| RseI | CAYNNNNRTG | 1 cut(s) | 480 |
| SaqAI | TTAA | 6 cut(s) | 30, 420, 429, 486, 537, 689 |
| SatI | GCNGC | 1 cut(s) | 211 |
| Sau3AI | GATC | 2 cut(s) | 380, 436 |
| SchI | GAGTC | 1 cut(s) | 750 |
| ScrFI | CCNGG | 3 cut(s) | 45, 709, 749 |
| SduI | GDGCHC | 3 cut(s) | 28, 107, 392 |
| SfaNI | GCATC | 1 cut(s) | 513 |
| SfcI | CTRYAG | 2 cut(s) | 319, 655 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 480 |
| SmlI | CTYRAG | 1 cut(s) | 428 |
| SmoI | CTYRAG | 1 cut(s) | 428 |
| Sse9I | AATT | 5 cut(s) | 193, 364, 538, 647, 702 |
| SsiI | CCGC | 2 cut(s) | 211, 718 |
| StyD4I | CCNGG | 3 cut(s) | 43, 707, 747 |
| TaaI | ACNGT | 3 cut(s) | 532, 555, 725 |
| TaiI | ACGT | 1 cut(s) | 164 |
| TaqI | TCGA | 1 cut(s) | 349 |
| TasI | AATT | 5 cut(s) | 193, 364, 538, 647, 702 |
| TauI | GCSGC | 1 cut(s) | 213 |
| TfiI | GAWTC | 2 cut(s) | 260, 618 |
| Tru1I | TTAA | 6 cut(s) | 30, 420, 429, 486, 537, 689 |
| Tru9I | TTAA | 6 cut(s) | 30, 420, 429, 486, 537, 689 |
| TscAI | CASTG | 1 cut(s) | 149 |
| TseFI | GTSAC | 1 cut(s) | 740 |
| Tsp45I | GTSAC | 1 cut(s) | 740 |
| TspDTI | ATGAA | 3 cut(s) | 128, 516, 610 |
| TspRI | CASTG | 1 cut(s) | 149 |
| Vha464I | CTTAAG | 1 cut(s) | 428 |
| VneI | GTGCAC | 1 cut(s) | 24 |
| XapI | RAATTY | 2 cut(s) | 647, 702 |
| XceI | RCATGY | 1 cut(s) | 454 |
| XmiI | GTMKAC | 1 cut(s) | 600 |
| XmnI | GAANNNNTTC | 1 cut(s) | 647 |
| Zsp2I | ATGCAT | 2 cut(s) | 281, 456 |