E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 624 |
| AciI | CCGC | 1 cut(s) | 747 |
| AclWI | GGATC | 1 cut(s) | 351 |
| AcoI | YGGCCR | 1 cut(s) | 229 |
| AcsI | RAATTY | 4 cut(s) | 132, 193, 363, 777 |
| AcuI | CTGAAG | 1 cut(s) | 908 |
| AcvI | CACGTG | 1 cut(s) | 282 |
| AcyI | GRCGYC | 1 cut(s) | 625 |
| AdeI | CACNNNGTG | 1 cut(s) | 160 |
| AfaI | GTAC | 1 cut(s) | 541 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 452, 690 |
| AflIII | ACRYGT | 1 cut(s) | 281 |
| AgsI | TTSAA | 1 cut(s) | 547 |
| AjnI | CCWGG | 3 cut(s) | 7, 118, 136 |
| AluBI | AGCT | 6 cut(s) | 113, 249, 403, 458, 763, 892 |
| AluI | AGCT | 6 cut(s) | 113, 249, 403, 458, 763, 892 |
| Alw21I | GWGCWC | 1 cut(s) | 267 |
| Alw26I | GTCTC | 3 cut(s) | 9, 45, 740 |
| Alw44I | GTGCAC | 1 cut(s) | 263 |
| AlwI | GGATC | 1 cut(s) | 351 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 62, 886 |
| Ama87I | CYCGRG | 2 cut(s) | 173, 244 |
| AoxI | GGCC | 2 cut(s) | 229, 681 |
| ApaLI | GTGCAC | 1 cut(s) | 263 |
| ApeKI | GCWGC | 1 cut(s) | 113 |
| ApoI | RAATTY | 4 cut(s) | 132, 193, 363, 777 |
| AspA2I | CCTAGG | 1 cut(s) | 782 |
| AspLEI | GCGC | 1 cut(s) | 627 |
| AsuHPI | GGTGA | 3 cut(s) | 95, 206, 713 |
| AvaI | CYCGRG | 2 cut(s) | 173, 244 |
| AvrII | CCTAGG | 1 cut(s) | 782 |
| BaeGI | GKGCMC | 1 cut(s) | 267 |
| BalI | TGGCCA | 1 cut(s) | 231 |
| BanI | GGYRCC | 1 cut(s) | 624 |
| BbrPI | CACGTG | 1 cut(s) | 282 |
| Bbv12I | GWGCWC | 1 cut(s) | 267 |
| BbvI | GCAGC | 1 cut(s) | 100 |
| BccI | CCATC | 2 cut(s) | 154, 821 |
| BciT130I | CCWGG | 3 cut(s) | 9, 120, 138 |
| BclI | TGATCA | 1 cut(s) | 511 |
| BcoDI | GTCTC | 3 cut(s) | 9, 45, 740 |
| BfaI | CTAG | 1 cut(s) | 783 |
| BfmI | CTRYAG | 1 cut(s) | 394 |
| BfoI | RGCGCY | 1 cut(s) | 628 |
| BisI | GCNGC | 2 cut(s) | 114, 748 |
| BlnI | CCTAGG | 1 cut(s) | 782 |
| BlsI | GCNGC | 2 cut(s) | 115, 749 |
| Bme1390I | CCNGG | 3 cut(s) | 9, 120, 138 |
| BmeT110I | CYCGRG | 2 cut(s) | 173, 244 |
| BmiI | GGNNCC | 1 cut(s) | 626 |
| BmrFI | CCNGG | 3 cut(s) | 9, 120, 138 |
| BmsI | GCATC | 3 cut(s) | 486, 508, 798 |
| BpmI | CTGGAG | 3 cut(s) | 30, 341, 474 |
| BsaAI | YACGTR | 1 cut(s) | 282 |
| BsaHI | GRCGYC | 1 cut(s) | 625 |
| BsaJI | CCNNGG | 3 cut(s) | 118, 782, 842 |
| BsaWI | WCCGGW | 1 cut(s) | 866 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 452, 690 |
| Bse1I | ACTGG | 4 cut(s) | 85, 217, 324, 628 |
| Bse3DI | GCAATG | 1 cut(s) | 295 |
| BseBI | CCWGG | 3 cut(s) | 9, 120, 138 |
| BseDI | CCNNGG | 3 cut(s) | 118, 782, 842 |
| BseGI | GGATG | 4 cut(s) | 34, 542, 603, 789 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 452, 690 |
| BseMI | GCAATG | 1 cut(s) | 295 |
| BseMII | CTCAG | 1 cut(s) | 27 |
| BseNI | ACTGG | 4 cut(s) | 85, 217, 324, 628 |
| BseSI | GKGCMC | 1 cut(s) | 267 |
| BseXI | GCAGC | 1 cut(s) | 100 |
| BsgI | GTGCAG | 1 cut(s) | 651 |
| BshFI | GGCC | 2 cut(s) | 231, 683 |
| BshNI | GGYRCC | 1 cut(s) | 624 |
| BsiHKAI | GWGCWC | 1 cut(s) | 267 |
| BsiHKCI | CYCGRG | 2 cut(s) | 173, 244 |
| BsiSI | CCGG | 1 cut(s) | 867 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 452, 690 |
| BsmAI | GTCTC | 3 cut(s) | 9, 45, 740 |
| BsmI | GAATGC | 1 cut(s) | 147 |
| BsnI | GGCC | 2 cut(s) | 231, 683 |
| BsoBI | CYCGRG | 2 cut(s) | 173, 244 |
| Bsp1286I | GDGCHC | 1 cut(s) | 267 |
| Bsp143I | GATC | 2 cut(s) | 343, 511 |
| BspACI | CCGC | 1 cut(s) | 747 |
| BspANI | GGCC | 2 cut(s) | 231, 683 |
| BspCNI | CTCAG | 1 cut(s) | 28 |
| BspLI | GGNNCC | 1 cut(s) | 626 |
| BspPI | GGATC | 1 cut(s) | 351 |
| BspT107I | GGYRCC | 1 cut(s) | 624 |
| BsrDI | GCAATG | 1 cut(s) | 295 |
| BsrI | ACTGG | 4 cut(s) | 85, 217, 324, 628 |
| BssECI | CCNNGG | 3 cut(s) | 118, 782, 842 |
| BssMI | GATC | 2 cut(s) | 343, 511 |
| BssNI | GRCGYC | 1 cut(s) | 625 |
| BssT1I | CCWWGG | 1 cut(s) | 782 |
| Bst2UI | CCWGG | 3 cut(s) | 9, 120, 138 |
| Bst4CI | ACNGT | 3 cut(s) | 272, 617, 821 |
| Bst6I | CTCTTC | 1 cut(s) | 863 |
| BstACI | GRCGYC | 1 cut(s) | 625 |
| BstBAI | YACGTR | 1 cut(s) | 282 |
| BstC8I | GCNNGC | 2 cut(s) | 401, 638 |
| BstDEI | CTNAG | 2 cut(s) | 36, 850 |
| BstF5I | GGATG | 4 cut(s) | 34, 542, 603, 789 |
| BstH2I | RGCGCY | 1 cut(s) | 628 |
| BstHHI | GCGC | 1 cut(s) | 627 |
| BstKTI | GATC | 2 cut(s) | 346, 514 |
| BstMAI | GTCTC | 3 cut(s) | 9, 45, 740 |
| BstMBI | GATC | 2 cut(s) | 343, 511 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 633, 753, 769 |
| BstNI | CCWGG | 3 cut(s) | 9, 120, 138 |
| BstNSI | RCATGY | 1 cut(s) | 529 |
| BstSCI | CCNGG | 3 cut(s) | 7, 118, 136 |
| BstSFI | CTRYAG | 1 cut(s) | 394 |
| BstSLI | GKGCMC | 1 cut(s) | 267 |
| BstV1I | GCAGC | 1 cut(s) | 100 |
| BstX2I | RGATCY | 1 cut(s) | 343 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 368 |
| BstYI | RGATCY | 1 cut(s) | 343 |
| BsuRI | GGCC | 2 cut(s) | 231, 683 |
| BtsCI | GGATG | 4 cut(s) | 34, 542, 603, 789 |
| BtsI | GCAGTG | 1 cut(s) | 757 |
| BtsIMutI | CAGTG | 5 cut(s) | 78, 224, 317, 757, 922 |
| Cac8I | GCNNGC | 2 cut(s) | 401, 638 |
| CaiI | CAGNNNCTG | 2 cut(s) | 62, 886 |
| CfoI | GCGC | 1 cut(s) | 627 |
| Csp6I | GTAC | 1 cut(s) | 540 |
| CviAII | CATG | 8 cut(s) | 164, 190, 410, 526, 530, 594, 679, 697 |
| CviQI | GTAC | 1 cut(s) | 540 |
| DdeI | CTNAG | 2 cut(s) | 36, 850 |
| DinI | GGCGCC | 1 cut(s) | 626 |
| DpnI | GATC | 2 cut(s) | 345, 513 |
| DpnII | GATC | 2 cut(s) | 343, 511 |
| DraIII | CACNNNGTG | 1 cut(s) | 160 |
| EaeI | YGGCCR | 1 cut(s) | 229 |
| Eam1104I | CTCTTC | 1 cut(s) | 863 |
| EarI | CTCTTC | 1 cut(s) | 863 |
| Eco130I | CCWWGG | 1 cut(s) | 782 |
| Eco32I | GATATC | 1 cut(s) | 481 |
| Eco57I | CTGAAG | 1 cut(s) | 908 |
| Eco72I | CACGTG | 1 cut(s) | 282 |
| Eco88I | CYCGRG | 2 cut(s) | 173, 244 |
| EcoRI | GAATTC | 2 cut(s) | 132, 777 |
| EcoRII | CCWGG | 3 cut(s) | 7, 118, 136 |
| EcoRV | GATATC | 1 cut(s) | 481 |
| EcoT14I | CCWWGG | 1 cut(s) | 782 |
| EcoT22I | ATGCAT | 2 cut(s) | 501, 531 |
| EgeI | GGCGCC | 1 cut(s) | 626 |
| EheI | GGCGCC | 1 cut(s) | 626 |
| ErhI | CCWWGG | 1 cut(s) | 782 |
| FaeI | CATG | 8 cut(s) | 167, 193, 413, 529, 533, 597, 682, 700 |
| FatI | CATG | 8 cut(s) | 163, 189, 409, 525, 529, 593, 678, 696 |
| FbaI | TGATCA | 1 cut(s) | 511 |
| Fnu4HI | GCNGC | 2 cut(s) | 114, 748 |
| FokI | GGATG | 4 cut(s) | 41, 549, 610, 776 |
| Fsp4HI | GCNGC | 2 cut(s) | 114, 748 |
| FspBI | CTAG | 1 cut(s) | 783 |
| GlaI | GCGC | 1 cut(s) | 626 |
| GluI | GCNGC | 2 cut(s) | 114, 748 |
| GsuI | CTGGAG | 3 cut(s) | 30, 341, 474 |
| HaeII | RGCGCY | 1 cut(s) | 628 |
| HaeIII | GGCC | 2 cut(s) | 231, 683 |
| HapII | CCGG | 1 cut(s) | 867 |
| HhaI | GCGC | 1 cut(s) | 627 |
| Hin1I | GRCGYC | 1 cut(s) | 625 |
| Hin1II | CATG | 8 cut(s) | 167, 193, 413, 529, 533, 597, 682, 700 |
| Hin6I | GCGC | 1 cut(s) | 625 |
| HinP1I | GCGC | 1 cut(s) | 625 |
| HincII | GTYRAC | 3 cut(s) | 523, 583, 603 |
| HindII | GTYRAC | 3 cut(s) | 523, 583, 603 |
| HindIII | AAGCTT | 1 cut(s) | 761 |
| HinfI | GANTC | 3 cut(s) | 38, 59, 335 |
| HpaII | CCGG | 1 cut(s) | 867 |
| HphI | GGTGA | 3 cut(s) | 95, 206, 713 |
| Hpy166II | GTNNAC | 5 cut(s) | 265, 523, 583, 603, 676 |
| Hpy188I | TCNGA | 4 cut(s) | 64, 107, 794, 843 |
| Hpy188III | TCNNGA | 3 cut(s) | 737, 815, 824 |
| Hpy8I | GTNNAC | 5 cut(s) | 265, 523, 583, 603, 676 |
| HpyAV | CCTTC | 2 cut(s) | 694, 698 |
| HpyCH4III | ACNGT | 3 cut(s) | 272, 617, 821 |
| HpyCH4IV | ACGT | 1 cut(s) | 281 |
| HpyCH4V | TGCA | 9 cut(s) | 56, 71, 163, 189, 265, 499, 529, 640, 668 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 633, 753, 769 |
| HpyF3I | CTNAG | 2 cut(s) | 36, 850 |
| HpySE526I | ACGT | 1 cut(s) | 281 |
| Hsp92I | GRCGYC | 1 cut(s) | 625 |
| Hsp92II | CATG | 8 cut(s) | 167, 193, 413, 529, 533, 597, 682, 700 |
| HspAI | GCGC | 1 cut(s) | 625 |
| KasI | GGCGCC | 1 cut(s) | 624 |
| Ksp22I | TGATCA | 1 cut(s) | 511 |
| Kzo9I | GATC | 2 cut(s) | 343, 511 |
| LmnI | GCTCC | 2 cut(s) | 455, 786 |
| Lsp1109I | GCAGC | 1 cut(s) | 100 |
| LweI | GCATC | 3 cut(s) | 486, 508, 798 |
| MaeI | CTAG | 1 cut(s) | 783 |
| MaeII | ACGT | 1 cut(s) | 281 |
| MaeIII | GTNAC | 2 cut(s) | 469, 815 |
| MalI | GATC | 2 cut(s) | 345, 513 |
| MboI | GATC | 2 cut(s) | 343, 511 |
| MboII | GAAGA | 3 cut(s) | 338, 850, 853 |
| MflI | RGATCY | 1 cut(s) | 343 |
| MhlI | GDGCHC | 1 cut(s) | 267 |
| MlsI | TGGCCA | 1 cut(s) | 231 |
| MluCI | AATT | 6 cut(s) | 132, 193, 210, 363, 722, 777 |
| MluNI | TGGCCA | 1 cut(s) | 231 |
| Mly113I | GGCGCC | 1 cut(s) | 625 |
| MlyI | GAGTC | 2 cut(s) | 47, 344 |
| MmeI | TCCRAC | 1 cut(s) | 508 |
| MnlI | CCTC | 6 cut(s) | 5, 19, 216, 640, 706, 837 |
| Mox20I | TGGCCA | 1 cut(s) | 231 |
| Mph1103I | ATGCAT | 2 cut(s) | 501, 531 |
| MscI | TGGCCA | 1 cut(s) | 231 |
| MseI | TTAA | 4 cut(s) | 197, 504, 561, 612 |
| MslI | CAYNNNNRTG | 1 cut(s) | 555 |
| Msp20I | TGGCCA | 1 cut(s) | 231 |
| MspI | CCGG | 1 cut(s) | 867 |
| MspR9I | CCNGG | 3 cut(s) | 9, 120, 138 |
| Mva1269I | GAATGC | 1 cut(s) | 147 |
| MvaI | CCWGG | 3 cut(s) | 9, 120, 138 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 633, 753, 769 |
| NarI | GGCGCC | 1 cut(s) | 625 |
| NdeII | GATC | 2 cut(s) | 343, 511 |
| NlaIII | CATG | 8 cut(s) | 167, 193, 413, 529, 533, 597, 682, 700 |
| NlaIV | GGNNCC | 1 cut(s) | 626 |
| NmuCI | GTSAC | 2 cut(s) | 469, 815 |
| NsiI | ATGCAT | 2 cut(s) | 501, 531 |
| NspI | RCATGY | 1 cut(s) | 529 |
| PaeR7I | CTCGAG | 2 cut(s) | 173, 244 |
| PctI | GAATGC | 1 cut(s) | 147 |
| PfeI | GAWTC | 1 cut(s) | 59 |
| PfoI | TCCNGGA | 2 cut(s) | 7, 136 |
| PkrI | GCNGC | 2 cut(s) | 115, 749 |
| PleI | GAGTC | 2 cut(s) | 46, 343 |
| PluTI | GGCGCC | 1 cut(s) | 628 |
| PmaCI | CACGTG | 1 cut(s) | 282 |
| PmlI | CACGTG | 1 cut(s) | 282 |
| PpsI | GAGTC | 2 cut(s) | 46, 343 |
| Ppu21I | YACGTR | 1 cut(s) | 282 |
| Psp6I | CCWGG | 3 cut(s) | 7, 118, 136 |
| PspCI | CACGTG | 1 cut(s) | 282 |
| PspGI | CCWGG | 3 cut(s) | 7, 118, 136 |
| PspN4I | GGNNCC | 1 cut(s) | 626 |
| PspXI | VCTCGAGB | 2 cut(s) | 173, 244 |
| PstNI | CAGNNNCTG | 2 cut(s) | 62, 886 |
| PsuI | RGATCY | 1 cut(s) | 343 |
| RsaI | GTAC | 1 cut(s) | 541 |
| RsaNI | GTAC | 1 cut(s) | 540 |
| RseI | CAYNNNNRTG | 1 cut(s) | 555 |
| SaqAI | TTAA | 4 cut(s) | 197, 504, 561, 612 |
| SatI | GCNGC | 2 cut(s) | 114, 748 |
| Sau3AI | GATC | 2 cut(s) | 343, 511 |
| SchI | GAGTC | 2 cut(s) | 47, 344 |
| ScrFI | CCNGG | 3 cut(s) | 9, 120, 138 |
| SduI | GDGCHC | 1 cut(s) | 267 |
| SfaNI | GCATC | 3 cut(s) | 486, 508, 798 |
| SfcI | CTRYAG | 1 cut(s) | 394 |
| SfoI | GGCGCC | 1 cut(s) | 626 |
| Sfr274I | CTCGAG | 2 cut(s) | 173, 244 |
| SlaI | CTCGAG | 2 cut(s) | 173, 244 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 555 |
| SmlI | CTYRAG | 2 cut(s) | 173, 244 |
| SmoI | CTYRAG | 2 cut(s) | 173, 244 |
| Sse9I | AATT | 6 cut(s) | 132, 193, 210, 363, 722, 777 |
| SsiI | CCGC | 1 cut(s) | 747 |
| SspDI | GGCGCC | 1 cut(s) | 624 |
| SspMI | CTAG | 1 cut(s) | 783 |
| StyD4I | CCNGG | 3 cut(s) | 7, 118, 136 |
| StyI | CCWWGG | 1 cut(s) | 782 |
| TaaI | ACNGT | 3 cut(s) | 272, 617, 821 |
| TaiI | ACGT | 1 cut(s) | 284 |
| TaqI | TCGA | 3 cut(s) | 174, 245, 738 |
| TasI | AATT | 6 cut(s) | 132, 193, 210, 363, 722, 777 |
| TatI | WGTACW | 1 cut(s) | 539 |
| TauI | GCSGC | 1 cut(s) | 750 |
| TfiI | GAWTC | 1 cut(s) | 59 |
| Tru1I | TTAA | 4 cut(s) | 197, 504, 561, 612 |
| Tru9I | TTAA | 4 cut(s) | 197, 504, 561, 612 |
| TscAI | CASTG | 4 cut(s) | 85, 224, 324, 757 |
| TseFI | GTSAC | 2 cut(s) | 469, 815 |
| TseI | GCWGC | 1 cut(s) | 113 |
| Tsp45I | GTSAC | 2 cut(s) | 469, 815 |
| TspDTI | ATGAA | 3 cut(s) | 206, 306, 685 |
| TspGWI | ACGGA | 1 cut(s) | 549 |
| TspRI | CASTG | 4 cut(s) | 85, 224, 324, 757 |
| VneI | GTGCAC | 1 cut(s) | 263 |
| XapI | RAATTY | 4 cut(s) | 132, 193, 363, 777 |
| XceI | RCATGY | 1 cut(s) | 529 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 224 |
| XhoI | CTCGAG | 2 cut(s) | 173, 244 |
| XmaJI | CCTAGG | 1 cut(s) | 782 |
| XspI | CTAG | 1 cut(s) | 783 |
| Zsp2I | ATGCAT | 2 cut(s) | 501, 531 |