E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 522 |
| AciI | CCGC | 1 cut(s) | 666 |
| AclWI | GGATC | 1 cut(s) | 351 |
| AcoI | YGGCCR | 3 cut(s) | 229, 622, 904 |
| AcsI | RAATTY | 2 cut(s) | 363, 777 |
| AcuI | CTGAAG | 1 cut(s) | 908 |
| AfaI | GTAC | 2 cut(s) | 437, 541 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 452, 808 |
| AgeI | ACCGGT | 1 cut(s) | 670 |
| AgsI | TTSAA | 4 cut(s) | 547, 584, 613, 740 |
| AjnI | CCWGG | 2 cut(s) | 7, 136 |
| AluBI | AGCT | 8 cut(s) | 113, 249, 403, 441, 458, 763, 886, 892 |
| AluI | AGCT | 8 cut(s) | 113, 249, 403, 441, 458, 763, 886, 892 |
| Alw21I | GWGCWC | 1 cut(s) | 267 |
| Alw26I | GTCTC | 2 cut(s) | 9, 342 |
| Alw44I | GTGCAC | 1 cut(s) | 263 |
| AlwI | GGATC | 1 cut(s) | 351 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 62, 886 |
| Ama87I | CYCGRG | 3 cut(s) | 173, 244, 865 |
| AoxI | GGCC | 3 cut(s) | 229, 622, 904 |
| ApaLI | GTGCAC | 1 cut(s) | 263 |
| ApeKI | GCWGC | 3 cut(s) | 113, 116, 441 |
| ApoI | RAATTY | 2 cut(s) | 363, 777 |
| AsiGI | ACCGGT | 1 cut(s) | 670 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 722 |
| AspS9I | GGNCC | 1 cut(s) | 811 |
| AsuHPI | GGTGA | 1 cut(s) | 206 |
| AvaI | CYCGRG | 3 cut(s) | 173, 244, 865 |
| AvaII | GGWCC | 1 cut(s) | 811 |
| AxyI | CCTNAGG | 1 cut(s) | 689 |
| BaeGI | GKGCMC | 1 cut(s) | 267 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 51, 84 |
| BalI | TGGCCA | 1 cut(s) | 231 |
| Bbv12I | GWGCWC | 1 cut(s) | 267 |
| BbvI | GCAGC | 3 cut(s) | 100, 103, 428 |
| BceAI | ACGGC | 2 cut(s) | 609, 891 |
| BciT130I | CCWGG | 2 cut(s) | 9, 138 |
| BciVI | GTATCC | 1 cut(s) | 800 |
| BclI | TGATCA | 1 cut(s) | 511 |
| BcoDI | GTCTC | 2 cut(s) | 9, 342 |
| BfmI | CTRYAG | 1 cut(s) | 394 |
| BfuI | GTATCC | 1 cut(s) | 800 |
| BisI | GCNGC | 3 cut(s) | 114, 117, 442 |
| BlsI | GCNGC | 3 cut(s) | 115, 118, 443 |
| BmcAI | AGTACT | 1 cut(s) | 541 |
| Bme1390I | CCNGG | 2 cut(s) | 9, 138 |
| Bme18I | GGWCC | 1 cut(s) | 811 |
| BmeT110I | CYCGRG | 3 cut(s) | 173, 244, 865 |
| BmgT120I | GGNCC | 1 cut(s) | 811 |
| BmiI | GGNNCC | 1 cut(s) | 664 |
| BmrFI | CCNGG | 2 cut(s) | 9, 138 |
| BmsI | GCATC | 2 cut(s) | 486, 508 |
| BpmI | CTGGAG | 4 cut(s) | 30, 341, 474, 495 |
| BsaJI | CCNNGG | 1 cut(s) | 782 |
| BsaWI | WCCGGW | 1 cut(s) | 670 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 452, 808 |
| Bse118I | RCCGGY | 2 cut(s) | 670, 902 |
| Bse1I | ACTGG | 3 cut(s) | 217, 324, 478 |
| Bse21I | CCTNAGG | 1 cut(s) | 689 |
| BseBI | CCWGG | 2 cut(s) | 9, 138 |
| BseDI | CCNNGG | 1 cut(s) | 782 |
| BseGI | GGATG | 1 cut(s) | 603 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 452, 808 |
| BseNI | ACTGG | 3 cut(s) | 217, 324, 478 |
| BseSI | GKGCMC | 1 cut(s) | 267 |
| BseX3I | CGGCCG | 1 cut(s) | 904 |
| BseXI | GCAGC | 3 cut(s) | 100, 103, 428 |
| Bsh1285I | CGRYCG | 1 cut(s) | 907 |
| BshFI | GGCC | 3 cut(s) | 231, 624, 906 |
| BshTI | ACCGGT | 1 cut(s) | 670 |
| BsiEI | CGRYCG | 1 cut(s) | 907 |
| BsiHKAI | GWGCWC | 1 cut(s) | 267 |
| BsiHKCI | CYCGRG | 3 cut(s) | 173, 244, 865 |
| BsiSI | CCGG | 2 cut(s) | 671, 903 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 452, 808 |
| BsmAI | GTCTC | 2 cut(s) | 9, 342 |
| BsmI | GAATGC | 2 cut(s) | 650, 683 |
| BsnI | GGCC | 3 cut(s) | 231, 624, 906 |
| BsoBI | CYCGRG | 3 cut(s) | 173, 244, 865 |
| Bsp1286I | GDGCHC | 1 cut(s) | 267 |
| Bsp143I | GATC | 4 cut(s) | 85, 166, 343, 511 |
| BspACI | CCGC | 1 cut(s) | 666 |
| BspANI | GGCC | 3 cut(s) | 231, 624, 906 |
| BspLI | GGNNCC | 1 cut(s) | 664 |
| BspPI | GGATC | 1 cut(s) | 351 |
| BsrFI | RCCGGY | 2 cut(s) | 670, 902 |
| BsrI | ACTGG | 3 cut(s) | 217, 324, 478 |
| BssAI | RCCGGY | 2 cut(s) | 670, 902 |
| BssECI | CCNNGG | 1 cut(s) | 782 |
| BssMI | GATC | 4 cut(s) | 85, 166, 343, 511 |
| Bst2UI | CCWGG | 2 cut(s) | 9, 138 |
| Bst4CI | ACNGT | 3 cut(s) | 272, 617, 821 |
| Bst6I | CTCTTC | 1 cut(s) | 863 |
| BstC8I | GCNNGC | 3 cut(s) | 401, 638, 904 |
| BstDEI | CTNAG | 3 cut(s) | 89, 689, 850 |
| BstF5I | GGATG | 1 cut(s) | 603 |
| BstKTI | GATC | 4 cut(s) | 88, 169, 346, 514 |
| BstMAI | GTCTC | 2 cut(s) | 9, 342 |
| BstMBI | GATC | 4 cut(s) | 85, 166, 343, 511 |
| BstMCI | CGRYCG | 1 cut(s) | 907 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 769 |
| BstNI | CCWGG | 2 cut(s) | 9, 138 |
| BstNSI | RCATGY | 1 cut(s) | 529 |
| BstSCI | CCNGG | 2 cut(s) | 7, 136 |
| BstSFI | CTRYAG | 1 cut(s) | 394 |
| BstSLI | GKGCMC | 1 cut(s) | 267 |
| BstV1I | GCAGC | 3 cut(s) | 100, 103, 428 |
| BstX2I | RGATCY | 1 cut(s) | 343 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 368 |
| BstYI | RGATCY | 1 cut(s) | 343 |
| BstZI | CGGCCG | 1 cut(s) | 904 |
| Bsu36I | CCTNAGG | 1 cut(s) | 689 |
| BsuI | GTATCC | 1 cut(s) | 800 |
| BsuRI | GGCC | 3 cut(s) | 231, 624, 906 |
| BtgZI | GCGATG | 1 cut(s) | 717 |
| BtsCI | GGATG | 1 cut(s) | 603 |
| BtsI | GCAGTG | 2 cut(s) | 78, 757 |
| BtsIMutI | CAGTG | 7 cut(s) | 78, 224, 317, 471, 751, 757, 922 |
| Cac8I | GCNNGC | 3 cut(s) | 401, 638, 904 |
| CaiI | CAGNNNCTG | 2 cut(s) | 62, 886 |
| Cfr10I | RCCGGY | 2 cut(s) | 670, 902 |
| Cfr13I | GGNCC | 1 cut(s) | 811 |
| Csp6I | GTAC | 2 cut(s) | 436, 540 |
| CspAI | ACCGGT | 1 cut(s) | 670 |
| CviAII | CATG | 9 cut(s) | 164, 190, 410, 526, 530, 594, 679, 697, 766 |
| CviQI | GTAC | 2 cut(s) | 436, 540 |
| DdeI | CTNAG | 3 cut(s) | 89, 689, 850 |
| DpnI | GATC | 4 cut(s) | 87, 168, 345, 513 |
| DpnII | GATC | 4 cut(s) | 85, 166, 343, 511 |
| EaeI | YGGCCR | 3 cut(s) | 229, 622, 904 |
| EagI | CGGCCG | 1 cut(s) | 904 |
| Eam1104I | CTCTTC | 1 cut(s) | 863 |
| EarI | CTCTTC | 1 cut(s) | 863 |
| EclXI | CGGCCG | 1 cut(s) | 904 |
| Eco32I | GATATC | 1 cut(s) | 481 |
| Eco47I | GGWCC | 1 cut(s) | 811 |
| Eco52I | CGGCCG | 1 cut(s) | 904 |
| Eco57I | CTGAAG | 1 cut(s) | 908 |
| Eco81I | CCTNAGG | 1 cut(s) | 689 |
| Eco88I | CYCGRG | 3 cut(s) | 173, 244, 865 |
| EcoRI | GAATTC | 1 cut(s) | 777 |
| EcoRII | CCWGG | 2 cut(s) | 7, 136 |
| EcoRV | GATATC | 1 cut(s) | 481 |
| EcoT22I | ATGCAT | 2 cut(s) | 501, 531 |
| FaeI | CATG | 9 cut(s) | 167, 193, 413, 529, 533, 597, 682, 700, 769 |
| FatI | CATG | 9 cut(s) | 163, 189, 409, 525, 529, 593, 678, 696, 765 |
| FbaI | TGATCA | 1 cut(s) | 511 |
| FblI | GTMKAC | 1 cut(s) | 522 |
| Fnu4HI | GCNGC | 3 cut(s) | 114, 117, 442 |
| FokI | GGATG | 1 cut(s) | 610 |
| Fsp4HI | GCNGC | 3 cut(s) | 114, 117, 442 |
| GluI | GCNGC | 3 cut(s) | 114, 117, 442 |
| GsuI | CTGGAG | 4 cut(s) | 30, 341, 474, 495 |
| HaeIII | GGCC | 3 cut(s) | 231, 624, 906 |
| HapII | CCGG | 2 cut(s) | 671, 903 |
| Hin1II | CATG | 9 cut(s) | 167, 193, 413, 529, 533, 597, 682, 700, 769 |
| HincII | GTYRAC | 2 cut(s) | 523, 603 |
| HindII | GTYRAC | 2 cut(s) | 523, 603 |
| HinfI | GANTC | 2 cut(s) | 38, 335 |
| HpaII | CCGG | 2 cut(s) | 671, 903 |
| HphI | GGTGA | 1 cut(s) | 206 |
| Hpy166II | GTNNAC | 4 cut(s) | 265, 523, 603, 676 |
| Hpy188I | TCNGA | 5 cut(s) | 107, 260, 786, 794, 843 |
| Hpy188III | TCNNGA | 3 cut(s) | 341, 737, 824 |
| Hpy8I | GTNNAC | 4 cut(s) | 265, 523, 603, 676 |
| HpyCH4III | ACNGT | 3 cut(s) | 272, 617, 821 |
| HpyCH4V | TGCA | 8 cut(s) | 56, 71, 163, 189, 265, 499, 529, 640 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 769 |
| HpyF3I | CTNAG | 3 cut(s) | 89, 689, 850 |
| Hsp92II | CATG | 9 cut(s) | 167, 193, 413, 529, 533, 597, 682, 700, 769 |
| KroI | GCCGGC | 1 cut(s) | 902 |
| KroNI | GCCGGC | 1 cut(s) | 904 |
| Ksp22I | TGATCA | 1 cut(s) | 511 |
| Kzo9I | GATC | 4 cut(s) | 85, 166, 343, 511 |
| LmnI | GCTCC | 2 cut(s) | 455, 883 |
| Lsp1109I | GCAGC | 3 cut(s) | 100, 103, 428 |
| LweI | GCATC | 2 cut(s) | 486, 508 |
| MaeIII | GTNAC | 4 cut(s) | 31, 422, 469, 815 |
| MalI | GATC | 4 cut(s) | 87, 168, 345, 513 |
| MboI | GATC | 4 cut(s) | 85, 166, 343, 511 |
| MboII | GAAGA | 4 cut(s) | 38, 338, 850, 853 |
| MflI | RGATCY | 1 cut(s) | 343 |
| MhlI | GDGCHC | 1 cut(s) | 267 |
| MlsI | TGGCCA | 1 cut(s) | 231 |
| MluCI | AATT | 7 cut(s) | 210, 363, 485, 606, 722, 777, 834 |
| MluNI | TGGCCA | 1 cut(s) | 231 |
| MlyI | GAGTC | 2 cut(s) | 47, 344 |
| MnlI | CCTC | 5 cut(s) | 5, 640, 706, 792, 837 |
| Mox20I | TGGCCA | 1 cut(s) | 231 |
| Mph1103I | ATGCAT | 2 cut(s) | 501, 531 |
| MroNI | GCCGGC | 1 cut(s) | 902 |
| MroXI | GAANNNNTTC | 1 cut(s) | 722 |
| MscI | TGGCCA | 1 cut(s) | 231 |
| MseI | TTAA | 3 cut(s) | 197, 504, 561 |
| MslI | CAYNNNNRTG | 1 cut(s) | 555 |
| Msp20I | TGGCCA | 1 cut(s) | 231 |
| MspA1I | CMGCKG | 1 cut(s) | 441 |
| MspI | CCGG | 2 cut(s) | 671, 903 |
| MspR9I | CCNGG | 2 cut(s) | 9, 138 |
| Mva1269I | GAATGC | 2 cut(s) | 650, 683 |
| MvaI | CCWGG | 2 cut(s) | 9, 138 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 769 |
| NaeI | GCCGGC | 1 cut(s) | 904 |
| NdeII | GATC | 4 cut(s) | 85, 166, 343, 511 |
| NgoMIV | GCCGGC | 1 cut(s) | 902 |
| NlaIII | CATG | 9 cut(s) | 167, 193, 413, 529, 533, 597, 682, 700, 769 |
| NlaIV | GGNNCC | 1 cut(s) | 664 |
| NmuCI | GTSAC | 3 cut(s) | 31, 469, 815 |
| NsiI | ATGCAT | 2 cut(s) | 501, 531 |
| NspI | RCATGY | 1 cut(s) | 529 |
| PaeR7I | CTCGAG | 2 cut(s) | 173, 244 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 790 |
| PctI | GAATGC | 2 cut(s) | 650, 683 |
| PdiI | GCCGGC | 1 cut(s) | 904 |
| PdmI | GAANNNNTTC | 1 cut(s) | 722 |
| PflFI | GACNNNGTC | 1 cut(s) | 37 |
| PfoI | TCCNGGA | 2 cut(s) | 7, 136 |
| PinAI | ACCGGT | 1 cut(s) | 670 |
| PkrI | GCNGC | 3 cut(s) | 115, 118, 443 |
| PleI | GAGTC | 2 cut(s) | 46, 343 |
| PpsI | GAGTC | 2 cut(s) | 46, 343 |
| Psp6I | CCWGG | 2 cut(s) | 7, 136 |
| PspGI | CCWGG | 2 cut(s) | 7, 136 |
| PspN4I | GGNNCC | 1 cut(s) | 664 |
| PspPI | GGNCC | 1 cut(s) | 811 |
| PspXI | VCTCGAGB | 2 cut(s) | 173, 244 |
| PstNI | CAGNNNCTG | 2 cut(s) | 62, 886 |
| PsuI | RGATCY | 1 cut(s) | 343 |
| PsyI | GACNNNGTC | 1 cut(s) | 37 |
| PvuII | CAGCTG | 1 cut(s) | 441 |
| RsaI | GTAC | 2 cut(s) | 437, 541 |
| RsaNI | GTAC | 2 cut(s) | 436, 540 |
| RseI | CAYNNNNRTG | 1 cut(s) | 555 |
| SalI | GTCGAC | 1 cut(s) | 521 |
| SaqAI | TTAA | 3 cut(s) | 197, 504, 561 |
| SatI | GCNGC | 3 cut(s) | 114, 117, 442 |
| Sau3AI | GATC | 4 cut(s) | 85, 166, 343, 511 |
| Sau96I | GGNCC | 1 cut(s) | 811 |
| ScaI | AGTACT | 1 cut(s) | 541 |
| SchI | GAGTC | 2 cut(s) | 47, 344 |
| ScrFI | CCNGG | 2 cut(s) | 9, 138 |
| SduI | GDGCHC | 1 cut(s) | 267 |
| SfaNI | GCATC | 2 cut(s) | 486, 508 |
| SfcI | CTRYAG | 1 cut(s) | 394 |
| Sfr274I | CTCGAG | 2 cut(s) | 173, 244 |
| SinI | GGWCC | 1 cut(s) | 811 |
| SlaI | CTCGAG | 2 cut(s) | 173, 244 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 555 |
| SmlI | CTYRAG | 2 cut(s) | 173, 244 |
| SmoI | CTYRAG | 2 cut(s) | 173, 244 |
| Sse9I | AATT | 7 cut(s) | 210, 363, 485, 606, 722, 777, 834 |
| SsiI | CCGC | 1 cut(s) | 666 |
| StyD4I | CCNGG | 2 cut(s) | 7, 136 |
| TaaI | ACNGT | 3 cut(s) | 272, 617, 821 |
| TaqI | TCGA | 4 cut(s) | 174, 245, 286, 522 |
| TaqII | GACCGA | 1 cut(s) | 50 |
| TasI | AATT | 7 cut(s) | 210, 363, 485, 606, 722, 777, 834 |
| TatI | WGTACW | 1 cut(s) | 539 |
| Tru1I | TTAA | 3 cut(s) | 197, 504, 561 |
| Tru9I | TTAA | 3 cut(s) | 197, 504, 561 |
| TscAI | CASTG | 6 cut(s) | 85, 224, 324, 478, 751, 757 |
| TseFI | GTSAC | 3 cut(s) | 31, 469, 815 |
| TseI | GCWGC | 3 cut(s) | 113, 116, 441 |
| Tsp45I | GTSAC | 3 cut(s) | 31, 469, 815 |
| TspDTI | ATGAA | 1 cut(s) | 685 |
| TspGWI | ACGGA | 3 cut(s) | 423, 803, 843 |
| TspRI | CASTG | 6 cut(s) | 85, 224, 324, 478, 751, 757 |
| Tth111I | GACNNNGTC | 1 cut(s) | 37 |
| VneI | GTGCAC | 1 cut(s) | 263 |
| VpaK11BI | GGWCC | 1 cut(s) | 811 |
| XapI | RAATTY | 2 cut(s) | 363, 777 |
| XceI | RCATGY | 1 cut(s) | 529 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 224 |
| XhoI | CTCGAG | 2 cut(s) | 173, 244 |
| XmiI | GTMKAC | 1 cut(s) | 522 |
| XmnI | GAANNNNTTC | 1 cut(s) | 722 |
| ZrmI | AGTACT | 1 cut(s) | 541 |
| Zsp2I | ATGCAT | 2 cut(s) | 501, 531 |