E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 306 |
| AclWI | GGATC | 1 cut(s) | 94 |
| AcoI | YGGCCR | 2 cut(s) | 3, 535 |
| AcsI | RAATTY | 2 cut(s) | 353, 408 |
| AcuI | CTGAAG | 2 cut(s) | 56, 539 |
| AfaI | GTAC | 1 cut(s) | 172 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 83 |
| AflII | CTTAAG | 1 cut(s) | 134 |
| AgsI | TTSAA | 2 cut(s) | 178, 215 |
| AjnI | CCWGG | 2 cut(s) | 413, 453 |
| AluBI | AGCT | 2 cut(s) | 34, 360 |
| AluI | AGCT | 2 cut(s) | 34, 360 |
| Alw21I | GWGCWC | 1 cut(s) | 98 |
| AlwI | GGATC | 1 cut(s) | 94 |
| AoxI | GGCC | 2 cut(s) | 3, 535 |
| ApoI | RAATTY | 2 cut(s) | 353, 408 |
| AspLEI | GCGC | 1 cut(s) | 487 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BauI | CACGAG | 1 cut(s) | 41 |
| BbsI | GAAGAC | 1 cut(s) | 262 |
| Bbv12I | GWGCWC | 1 cut(s) | 98 |
| BccI | CCATC | 3 cut(s) | 158, 283, 452 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 35, 69 |
| BciT130I | CCWGG | 2 cut(s) | 415, 455 |
| BciVI | GTATCC | 1 cut(s) | 431 |
| BclI | TGATCA | 1 cut(s) | 142 |
| BfmI | CTRYAG | 1 cut(s) | 361 |
| BfoI | RGCGCY | 1 cut(s) | 488 |
| BfrI | CTTAAG | 1 cut(s) | 134 |
| BfuI | GTATCC | 1 cut(s) | 431 |
| Bme1390I | CCNGG | 2 cut(s) | 415, 455 |
| BmiI | GGNNCC | 1 cut(s) | 300 |
| BmrFI | CCNGG | 2 cut(s) | 415, 455 |
| BmsI | GCATC | 1 cut(s) | 219 |
| BpiI | GAAGAC | 1 cut(s) | 262 |
| BpmI | CTGGAG | 1 cut(s) | 285 |
| BsaJI | CCNNGG | 1 cut(s) | 413 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 409, 439 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 83 |
| Bse118I | RCCGGY | 1 cut(s) | 533 |
| Bse1I | ACTGG | 1 cut(s) | 302 |
| BseBI | CCWGG | 2 cut(s) | 415, 455 |
| BseDI | CCNNGG | 1 cut(s) | 413 |
| BseGI | GGATG | 3 cut(s) | 145, 234, 463 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 83 |
| BseMII | CTCAG | 2 cut(s) | 81, 333 |
| BseNI | ACTGG | 1 cut(s) | 302 |
| BseX3I | CGGCCG | 1 cut(s) | 535 |
| Bsh1285I | CGRYCG | 1 cut(s) | 538 |
| BshFI | GGCC | 2 cut(s) | 5, 537 |
| BsiEI | CGRYCG | 1 cut(s) | 538 |
| BsiHKAI | GWGCWC | 1 cut(s) | 98 |
| BsiSI | CCGG | 2 cut(s) | 534, 538 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 83 |
| BsnI | GGCC | 2 cut(s) | 5, 537 |
| Bsp1286I | GDGCHC | 1 cut(s) | 98 |
| Bsp143I | GATC | 2 cut(s) | 86, 142 |
| BspANI | GGCC | 2 cut(s) | 5, 537 |
| BspCNI | CTCAG | 2 cut(s) | 82, 334 |
| BspLI | GGNNCC | 1 cut(s) | 300 |
| BspPI | GGATC | 1 cut(s) | 94 |
| BspTI | CTTAAG | 1 cut(s) | 134 |
| BsrFI | RCCGGY | 1 cut(s) | 533 |
| BsrI | ACTGG | 1 cut(s) | 302 |
| BssAI | RCCGGY | 1 cut(s) | 533 |
| BssECI | CCNNGG | 1 cut(s) | 413 |
| BssMI | GATC | 2 cut(s) | 86, 142 |
| BssSI | CACGAG | 1 cut(s) | 41 |
| Bst2BI | CACGAG | 1 cut(s) | 41 |
| Bst2UI | CCWGG | 2 cut(s) | 415, 455 |
| Bst4CI | ACNGT | 3 cut(s) | 238, 261, 431 |
| BstAFI | CTTAAG | 1 cut(s) | 134 |
| BstC8I | GCNNGC | 2 cut(s) | 28, 32 |
| BstDEI | CTNAG | 2 cut(s) | 90, 342 |
| BstF5I | GGATG | 3 cut(s) | 145, 234, 463 |
| BstH2I | RGCGCY | 1 cut(s) | 488 |
| BstHHI | GCGC | 1 cut(s) | 487 |
| BstKTI | GATC | 2 cut(s) | 89, 145 |
| BstMBI | GATC | 2 cut(s) | 86, 142 |
| BstMCI | CGRYCG | 1 cut(s) | 538 |
| BstNI | CCWGG | 2 cut(s) | 415, 455 |
| BstNSI | RCATGY | 1 cut(s) | 160 |
| BstSCI | CCNGG | 2 cut(s) | 413, 453 |
| BstSFI | CTRYAG | 1 cut(s) | 361 |
| BstV2I | GAAGAC | 1 cut(s) | 262 |
| BstX2I | RGATCY | 1 cut(s) | 86 |
| BstYI | RGATCY | 1 cut(s) | 86 |
| BstZI | CGGCCG | 1 cut(s) | 535 |
| BsuI | GTATCC | 1 cut(s) | 431 |
| BsuRI | GGCC | 2 cut(s) | 5, 537 |
| BtgZI | GCGATG | 1 cut(s) | 348 |
| BtsCI | GGATG | 3 cut(s) | 145, 234, 463 |
| Cac8I | GCNNGC | 2 cut(s) | 28, 32 |
| CfoI | GCGC | 1 cut(s) | 487 |
| Cfr10I | RCCGGY | 1 cut(s) | 533 |
| Csp6I | GTAC | 1 cut(s) | 171 |
| CviAII | CATG | 5 cut(s) | 157, 161, 206, 310, 328 |
| CviJI | RGCY | 8 cut(s) | 5, 34, 281, 299, 314, 360, 453, 537 |
| CviKI_1 | RGCY | 8 cut(s) | 5, 34, 281, 299, 314, 360, 453, 537 |
| CviQI | GTAC | 1 cut(s) | 171 |
| DdeI | CTNAG | 2 cut(s) | 90, 342 |
| DpnI | GATC | 2 cut(s) | 88, 144 |
| DpnII | GATC | 2 cut(s) | 86, 142 |
| EaeI | YGGCCR | 2 cut(s) | 3, 535 |
| EagI | CGGCCG | 1 cut(s) | 535 |
| EclXI | CGGCCG | 1 cut(s) | 535 |
| Eco32I | GATATC | 1 cut(s) | 112 |
| Eco52I | CGGCCG | 1 cut(s) | 535 |
| Eco57I | CTGAAG | 2 cut(s) | 56, 539 |
| EcoRI | GAATTC | 1 cut(s) | 408 |
| EcoRII | CCWGG | 2 cut(s) | 413, 453 |
| EcoRV | GATATC | 1 cut(s) | 112 |
| EcoT22I | ATGCAT | 1 cut(s) | 162 |
| FaeI | CATG | 5 cut(s) | 160, 164, 209, 313, 331 |
| FatI | CATG | 5 cut(s) | 156, 160, 205, 309, 327 |
| FbaI | TGATCA | 1 cut(s) | 142 |
| FblI | GTMKAC | 1 cut(s) | 306 |
| FokI | GGATG | 3 cut(s) | 152, 241, 470 |
| GlaI | GCGC | 1 cut(s) | 486 |
| GsuI | CTGGAG | 1 cut(s) | 285 |
| HaeII | RGCGCY | 1 cut(s) | 488 |
| HaeIII | GGCC | 2 cut(s) | 5, 537 |
| HapII | CCGG | 2 cut(s) | 534, 538 |
| HhaI | GCGC | 1 cut(s) | 487 |
| Hin1II | CATG | 5 cut(s) | 160, 164, 209, 313, 331 |
| Hin6I | GCGC | 1 cut(s) | 485 |
| HinP1I | GCGC | 1 cut(s) | 485 |
| HincII | GTYRAC | 1 cut(s) | 154 |
| HindII | GTYRAC | 1 cut(s) | 154 |
| HinfI | GANTC | 2 cut(s) | 324, 462 |
| HpaII | CCGG | 2 cut(s) | 534, 538 |
| Hpy166II | GTNNAC | 3 cut(s) | 154, 171, 307 |
| Hpy188I | TCNGA | 2 cut(s) | 21, 91 |
| Hpy188III | TCNNGA | 2 cut(s) | 286, 446 |
| Hpy8I | GTNNAC | 3 cut(s) | 154, 171, 307 |
| HpyAV | CCTTC | 1 cut(s) | 184 |
| HpyCH4III | ACNGT | 3 cut(s) | 238, 261, 431 |
| HpyCH4V | TGCA | 4 cut(s) | 26, 160, 221, 271 |
| HpyF3I | CTNAG | 2 cut(s) | 90, 342 |
| Hsp92II | CATG | 5 cut(s) | 160, 164, 209, 313, 331 |
| HspAI | GCGC | 1 cut(s) | 485 |
| Ksp22I | TGATCA | 1 cut(s) | 142 |
| Kzo9I | GATC | 2 cut(s) | 86, 142 |
| LmnI | GCTCC | 1 cut(s) | 304 |
| LweI | GCATC | 1 cut(s) | 219 |
| MaeIII | GTNAC | 3 cut(s) | 100, 425, 446 |
| MalI | GATC | 2 cut(s) | 88, 144 |
| MboI | GATC | 2 cut(s) | 86, 142 |
| MboII | GAAGA | 1 cut(s) | 267 |
| MfeI | CAATTG | 1 cut(s) | 70 |
| MflI | RGATCY | 1 cut(s) | 86 |
| MhlI | GDGCHC | 1 cut(s) | 98 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 4 cut(s) | 70, 244, 353, 408 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 1 cut(s) | 456 |
| MnlI | CCTC | 4 cut(s) | 271, 337, 364, 519 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| Mph1103I | ATGCAT | 1 cut(s) | 162 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 5 cut(s) | 126, 135, 192, 243, 395 |
| MslI | CAYNNNNRTG | 1 cut(s) | 186 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspCI | CTTAAG | 1 cut(s) | 134 |
| MspI | CCGG | 2 cut(s) | 534, 538 |
| MspR9I | CCNGG | 2 cut(s) | 415, 455 |
| MunI | CAATTG | 1 cut(s) | 70 |
| MvaI | CCWGG | 2 cut(s) | 415, 455 |
| NdeII | GATC | 2 cut(s) | 86, 142 |
| NlaIII | CATG | 5 cut(s) | 160, 164, 209, 313, 331 |
| NlaIV | GGNNCC | 1 cut(s) | 300 |
| NmuCI | GTSAC | 2 cut(s) | 425, 446 |
| NsiI | ATGCAT | 1 cut(s) | 162 |
| NspI | RCATGY | 1 cut(s) | 160 |
| PfeI | GAWTC | 1 cut(s) | 324 |
| PleI | GAGTC | 1 cut(s) | 456 |
| PpsI | GAGTC | 1 cut(s) | 456 |
| Psp6I | CCWGG | 2 cut(s) | 413, 453 |
| PspGI | CCWGG | 2 cut(s) | 413, 453 |
| PspN4I | GGNNCC | 1 cut(s) | 300 |
| PsuI | RGATCY | 1 cut(s) | 86 |
| RsaI | GTAC | 1 cut(s) | 172 |
| RsaNI | GTAC | 1 cut(s) | 171 |
| RseI | CAYNNNNRTG | 1 cut(s) | 186 |
| SaqAI | TTAA | 5 cut(s) | 126, 135, 192, 243, 395 |
| Sau3AI | GATC | 2 cut(s) | 86, 142 |
| SchI | GAGTC | 1 cut(s) | 456 |
| ScrFI | CCNGG | 2 cut(s) | 415, 455 |
| SduI | GDGCHC | 1 cut(s) | 98 |
| SetI | ASST | 5 cut(s) | 36, 153, 176, 362, 375 |
| SfaNI | GCATC | 1 cut(s) | 219 |
| SfcI | CTRYAG | 1 cut(s) | 361 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 186 |
| SmlI | CTYRAG | 1 cut(s) | 134 |
| SmoI | CTYRAG | 1 cut(s) | 134 |
| Sse9I | AATT | 4 cut(s) | 70, 244, 353, 408 |
| StyD4I | CCNGG | 2 cut(s) | 413, 453 |
| TaaI | ACNGT | 3 cut(s) | 238, 261, 431 |
| TaqI | TCGA | 1 cut(s) | 55 |
| TasI | AATT | 4 cut(s) | 70, 244, 353, 408 |
| TfiI | GAWTC | 1 cut(s) | 324 |
| Tru1I | TTAA | 5 cut(s) | 126, 135, 192, 243, 395 |
| Tru9I | TTAA | 5 cut(s) | 126, 135, 192, 243, 395 |
| TseFI | GTSAC | 2 cut(s) | 425, 446 |
| Tsp45I | GTSAC | 2 cut(s) | 425, 446 |
| TspDTI | ATGAA | 2 cut(s) | 222, 316 |
| TspGWI | ACGGA | 1 cut(s) | 413 |
| Vha464I | CTTAAG | 1 cut(s) | 134 |
| XapI | RAATTY | 2 cut(s) | 353, 408 |
| XceI | RCATGY | 1 cut(s) | 160 |
| XmiI | GTMKAC | 1 cut(s) | 306 |
| Zsp2I | ATGCAT | 1 cut(s) | 162 |