AT2G44550

Contains the following InterPro domains Six-hairpin glycosidase (InterPro IPR012341), Glycoside hydrolase, family 9, active site (InterPro IPR018221), Six-hairpin glycosidase-like (InterPro IPR008928), Glycoside hydrolase, family 9 (InterPro IPR001701)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003358
Physical Location & Seq
Reverse (-)
18389381 .. 18391142
1762 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44550.1

Sequence Viewer

Length: 1473 bp
ATGTCTCAATTGAAAAATGGATCTTCACAATGCTTATGGACAAGTATTTGCATTGTGTTAATAGTAATGTCGATGGCTCGTGAAGCTGTCTCTACAAATTATGCAGAAGCTCTCAAAAACAGTCTTTTGTATTTCGAAGCTCAACGTTCTGGAAAATTGCCTCCGAACCAAAGAGTTACTTGGAGAGGAGATTCTGCTCTTAGAGACGGTTCTGATGCTCATATTGATCTCACCGGAGGGTACTATGATGCGGGAGACAACATGAAGTTTGGATTTCCGTTGGCGTTCACGACAACAATGCTAGCTTGGAGCAATATAGAGATGGCATCACAGCTTAGGGCCCACCACGAGAAGGGAAACGCTCTCAGGGCTCTCAAGTGGGCCACCGATTATCTCATTAAAGCCCATCCTCAGCCTAATGTCCTGTACGGACAAGTGGGTGAAGGAAACTCGGACCACAAATGCTGGATGAGACCTGAGGATATGACCACTCCGCGTACTTCATACCGCATCGATGCTCAACATCCAGGTTCTGACCTAGCAGGTGAGACAGCTGCAGCAATGGCGGCAGCGTCTATCGCCTTTGCACCATCTGATAAAGCCTATGCCAATATACTCATTGGTCATGCAAAAGATTTATTTGCATTCGCTAAGGCTCACCGTGGACTTTACCAAAACTCCATTCCTAACGCGGGTGGCTTCTACGCGAGCAGTGGATATGAAGATGAGTTGCTGTGGGCTGCAGCTTGGCTCCACCGTGCCACCAACGACCAGATTTATCTTGATTACCTAACAGAAGCAGAAACAGGAGGTCCAAGGACTGTTTTTGCCTGGGATGATAAGTTCGTGGGTGCACAAGTCTTGGTCGCCAAGCTAGCACTTGAAGGGAAAGTAGAGAGCAGTGAACAGATCGTAGAGTACAAGAGTATGGCGGAGCAGTTTATCTGCAACTGTGCTCAAAAAGGTGACAACAACGTTAAGAAAACCCCTGGCGGTTTGCTCTACTTCTTGCCGTGGAACAACCTCCAATACACCACAGCCGCTACTTTCGTCCTCTCAGCATATTCTAAATATCTTGAAGCCGCAAAAGCCTCCATCGACTGTCCCGATGGTGCTCTTCAAGCTTCTGATCTTCTCCAAGTCGCTCGTTCCCAGGTTGACTATATACTTGGGTCGAACCCCCAGAAAATGAGCTACATGGTTGGAGTTGGGACCAATTATCCCAAAAAACCACACCATAGAGCAGCCTCCATAGTATCGATCAGACAGGACAAAACACCAGTGACGTGTAGTGGAGGGTATGATAAATGGTATAACAATCCTGCACCAAACCCTAACGTTTTAGCGGGAGCAGTTGTCGGAGGACCCGACGATAACGATGTTTATGGAGATGAGAGGAGTAATTTCCAGCAAGCTGAGCCTGCGACGGTCACTACTGCTCCGCTTGTTGGTGTCTTGGCCCTCGTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

490

Amino Acids

53.45

Weight (kDa)

5.9

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 34 - 486 9.8e-163 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 533
AccII CGCG 3 cut(s) 496, 692, 707
AclI AACGTT 3 cut(s) 145, 975, 1338
AclWI GGATC 1 cut(s) 28
AfaI GTAC 4 cut(s) 242, 428, 499, 920
AfiI CCNNNNNNNGG 3 cut(s) 352, 692, 1448
AflIII ACRYGT 1 cut(s) 1286
AgsI TTSAA 4 cut(s) 13, 884, 1079, 1121
AjiI CACGTC 1 cut(s) 1287
AjnI CCWGG 4 cut(s) 526, 830, 988, 1152
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AloI GAACNNNNNNTCC 2 cut(s) 827, 859
Alw21I GWGCWC 3 cut(s) 856, 958, 1117
Alw26I GTCTC 6 cut(s) 9, 94, 198, 249, 466, 542
Alw44I GTGCAC 1 cut(s) 852
AlwI GGATC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 533
AoxI GGCC 3 cut(s) 339, 381, 1458
ApaI GGGCCC 1 cut(s) 343
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 6 cut(s) 554, 557, 569, 740, 743, 1244
AspS9I GGNCC 8 cut(s) 339, 340, 381, 454, 812, 1212, 1364, 1459
AsuHPI GGTGA 5 cut(s) 223, 452, 557, 650, 977
AsuII TTCGAA 1 cut(s) 135
AsuNHI GCTAGC 2 cut(s) 301, 874
AvaII GGWCC 4 cut(s) 454, 812, 1212, 1364
AxyI CCTNAGG 1 cut(s) 477
BaeGI GKGCMC 2 cut(s) 343, 856
BanII GRGCYC 2 cut(s) 343, 373
BauI CACGAG 2 cut(s) 78, 347
Bbv12I GWGCWC 3 cut(s) 856, 958, 1117
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 6 cut(s) 541, 569, 581, 727, 755, 1256
BccI CCATC 6 cut(s) 67, 316, 414, 598, 1103, 1103
BceAI ACGGC 1 cut(s) 997
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciT130I CCWGG 4 cut(s) 528, 832, 990, 1154
BcoDI GTCTC 6 cut(s) 9, 94, 198, 249, 466, 542
BfaI CTAG 3 cut(s) 302, 539, 875
BfmI CTRYAG 2 cut(s) 555, 741
BfuAI ACCTGC 1 cut(s) 533
BisI GCNGC 9 cut(s) 555, 558, 567, 570, 741, 744, 1041, 1083, 1245
BlpI GCTNAGC 1 cut(s) 1416
BlsI GCNGC 9 cut(s) 556, 559, 568, 571, 742, 745, 1042, 1084, 1246
Bme1390I CCNGG 4 cut(s) 528, 832, 990, 1154
Bme18I GGWCC 4 cut(s) 454, 812, 1212, 1364
BmgBI CACGTC 1 cut(s) 1287
BmgT120I GGNCC 8 cut(s) 339, 340, 381, 454, 812, 1212, 1364, 1459
BmiI GGNNCC 4 cut(s) 341, 752, 1213, 1366
BmrFI CCNGG 4 cut(s) 528, 832, 990, 1154
BmsI GCATC 5 cut(s) 205, 238, 335, 505, 519
BmtI GCTAGC 2 cut(s) 305, 878
Bpu10I CCTNAGC 3 cut(s) 335, 411, 651
Bpu1102I GCTNAGC 1 cut(s) 1416
Bpu14I TTCGAA 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 359
Bsa29I ATCGAT 2 cut(s) 513, 1259
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 6 cut(s) 661, 815, 831, 988, 1013, 1152
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 6 cut(s) 662, 692, 1341, 1371, 1423, 1453
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 692, 1448
Bse1I ACTGG 1 cut(s) 1280
Bse21I CCTNAGG 1 cut(s) 477
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 4 cut(s) 528, 832, 990, 1154
BseCI ATCGAT 2 cut(s) 513, 1259
BseDI CCNNGG 6 cut(s) 661, 815, 831, 988, 1013, 1152
BseGI GGATG 4 cut(s) 406, 474, 523, 841
BseLI CCNNNNNNNGG 3 cut(s) 352, 692, 1448
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 5 cut(s) 379, 425, 468, 1071, 1407
BseNI ACTGG 1 cut(s) 1280
BseRI GAGGAG 2 cut(s) 201, 1411
BseSI GKGCMC 2 cut(s) 343, 856
BseXI GCAGC 6 cut(s) 541, 569, 581, 727, 755, 1256
BsgI GTGCAG 1 cut(s) 1308
Bsh1236I CGCG 3 cut(s) 496, 692, 707
BshFI GGCC 3 cut(s) 341, 383, 1460
BshVI ATCGAT 2 cut(s) 513, 1259
BsiHKAI GWGCWC 3 cut(s) 856, 958, 1117
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 2 cut(s) 1089, 1225
BslI CCNNNNNNNGG 3 cut(s) 352, 692, 1448
BsmAI GTCTC 6 cut(s) 9, 94, 198, 249, 466, 542
BsmBI CGTCTC 1 cut(s) 198
BsmFI GGGAC 2 cut(s) 1089, 1225
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 3 cut(s) 341, 383, 1460
Bso31I GGTCTC 1 cut(s) 466
Bsp119I TTCGAA 1 cut(s) 135
Bsp120I GGGCCC 1 cut(s) 339
Bsp1286I GDGCHC 5 cut(s) 343, 373, 856, 958, 1117
Bsp143I GATC 5 cut(s) 20, 226, 909, 1129, 1260
Bsp1720I GCTNAGC 1 cut(s) 1416
BspANI GGCC 3 cut(s) 341, 383, 1460
BspCNI CTCAG 5 cut(s) 378, 424, 469, 1070, 1408
BspDI ATCGAT 2 cut(s) 513, 1259
BspFNI CGCG 3 cut(s) 496, 692, 707
BspLI GGNNCC 4 cut(s) 341, 752, 1213, 1366
BspMAI CTGCAG 2 cut(s) 559, 745
BspMI ACCTGC 1 cut(s) 533
BspOI GCTAGC 2 cut(s) 305, 878
BspPI GGATC 1 cut(s) 28
BspQI GCTCTTC 1 cut(s) 1122
BspT104I TTCGAA 1 cut(s) 135
BspTNI GGTCTC 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 567
BsrI ACTGG 1 cut(s) 1280
BssECI CCNNGG 6 cut(s) 661, 815, 831, 988, 1013, 1152
BssMI GATC 5 cut(s) 20, 226, 909, 1129, 1260
BssSI CACGAG 2 cut(s) 78, 347
BssT1I CCWWGG 1 cut(s) 815
Bst2BI CACGAG 2 cut(s) 78, 347
Bst2UI CCWGG 4 cut(s) 528, 832, 990, 1154
Bst4CI ACNGT 8 cut(s) 122, 209, 662, 758, 823, 953, 1103, 1429
Bst6I CTCTTC 1 cut(s) 1122
BstBI TTCGAA 1 cut(s) 135
BstC8I GCNNGC 5 cut(s) 303, 709, 876, 1413, 1422
BstDEI CTNAG 8 cut(s) 200, 335, 365, 411, 477, 651, 1057, 1416
BstDSI CCRYGG 2 cut(s) 661, 1013
BstF5I GGATG 4 cut(s) 406, 474, 523, 841
BstFNI CGCG 3 cut(s) 496, 692, 707
BstKTI GATC 5 cut(s) 23, 229, 912, 1132, 1263
BstMAI GTCTC 6 cut(s) 9, 94, 198, 249, 466, 542
BstMBI GATC 5 cut(s) 20, 226, 909, 1129, 1260
BstNI CCWGG 4 cut(s) 528, 832, 990, 1154
BstSCI CCNGG 4 cut(s) 526, 830, 988, 1152
BstSFI CTRYAG 2 cut(s) 555, 741
BstSLI GKGCMC 2 cut(s) 343, 856
BstUI CGCG 3 cut(s) 496, 692, 707
BstV1I GCAGC 6 cut(s) 541, 569, 581, 727, 755, 1256
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
Bsu15I ATCGAT 2 cut(s) 513, 1259
Bsu36I CCTNAGG 1 cut(s) 477
BsuRI GGCC 3 cut(s) 341, 383, 1460
BsuTUI ATCGAT 2 cut(s) 513, 1259
BtgI CCRYGG 2 cut(s) 661, 1013
BtrI CACGTC 1 cut(s) 1287
BtsCI GGATG 4 cut(s) 406, 474, 523, 841
BtsI GCAGTG 2 cut(s) 718, 907
BtsIMutI CAGTG 3 cut(s) 718, 907, 1287
BveI ACCTGC 1 cut(s) 533
Cac8I GCNNGC 5 cut(s) 303, 709, 876, 1413, 1422
CaiI CAGNNNCTG 1 cut(s) 533
Cfr13I GGNCC 8 cut(s) 339, 340, 381, 454, 812, 1212, 1364, 1459
ClaI ATCGAT 2 cut(s) 513, 1259
CseI GACGC 1 cut(s) 561
Csp6I GTAC 4 cut(s) 241, 427, 498, 919
CviAII CATG 3 cut(s) 262, 626, 1198
CviQI GTAC 4 cut(s) 241, 427, 498, 919
DdeI CTNAG 8 cut(s) 200, 335, 365, 411, 477, 651, 1057, 1416
DpnI GATC 5 cut(s) 22, 228, 911, 1131, 1262
DpnII GATC 5 cut(s) 20, 226, 909, 1129, 1260
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
EciI GGCGGA 1 cut(s) 947
Eco130I CCWWGG 1 cut(s) 815
Eco24I GRGCYC 2 cut(s) 343, 373
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 4 cut(s) 454, 812, 1212, 1364
Eco81I CCTNAGG 1 cut(s) 477
EcoO109I RGGNCCY 2 cut(s) 339, 1364
EcoRII CCWGG 4 cut(s) 526, 830, 988, 1152
EcoT14I CCWWGG 1 cut(s) 815
EcoT38I GRGCYC 2 cut(s) 343, 373
ErhI CCWWGG 1 cut(s) 815
Esp3I CGTCTC 1 cut(s) 198
FaeI CATG 3 cut(s) 265, 629, 1201
FalI AAGNNNNNCTT 2 cut(s) 163, 195
FaqI GGGAC 2 cut(s) 1089, 1225
FatI CATG 3 cut(s) 261, 625, 1197
FauI CCCGC 3 cut(s) 244, 685, 1339
Fnu4HI GCNGC 9 cut(s) 555, 558, 567, 570, 741, 744, 1041, 1083, 1245
FokI GGATG 4 cut(s) 393, 481, 510, 848
FriOI GRGCYC 2 cut(s) 343, 373
Fsp4HI GCNGC 9 cut(s) 555, 558, 567, 570, 741, 744, 1041, 1083, 1245
FspBI CTAG 3 cut(s) 302, 539, 875
GluI GCNGC 9 cut(s) 555, 558, 567, 570, 741, 744, 1041, 1083, 1245
HaeIII GGCC 3 cut(s) 341, 383, 1460
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 561
Hin1II CATG 3 cut(s) 265, 629, 1201
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HindIII AAGCTT 1 cut(s) 1122
HinfI GANTC 1 cut(s) 191
HpaII CCGG 1 cut(s) 234
HphI GGTGA 5 cut(s) 223, 452, 557, 650, 977
Hpy166II GTNNAC 5 cut(s) 288, 665, 854, 905, 1159
Hpy188I TCNGA 8 cut(s) 165, 214, 454, 535, 595, 1129, 1265, 1361
Hpy188III TCNNGA 6 cut(s) 80, 150, 289, 782, 1076, 1106
Hpy8I GTNNAC 5 cut(s) 288, 665, 854, 905, 1159
Hpy99I CGWCG 2 cut(s) 1373, 1429
HpyAV CCTTC 3 cut(s) 346, 437, 878
HpyCH4III ACNGT 8 cut(s) 122, 209, 662, 758, 823, 953, 1103, 1429
HpyCH4IV ACGT 4 cut(s) 145, 975, 1286, 1338
HpyF3I CTNAG 8 cut(s) 200, 335, 365, 411, 477, 651, 1057, 1416
HpySE526I ACGT 4 cut(s) 145, 975, 1286, 1338
Hsp92II CATG 3 cut(s) 265, 629, 1201
Kzo9I GATC 5 cut(s) 20, 226, 909, 1129, 1260
LguI GCTCTTC 1 cut(s) 1122
LmnI GCTCC 5 cut(s) 309, 756, 934, 1349, 1444
Lsp1109I GCAGC 6 cut(s) 541, 569, 581, 727, 755, 1256
LweI GCATC 5 cut(s) 205, 238, 335, 505, 519
MaeI CTAG 3 cut(s) 302, 539, 875
MaeII ACGT 4 cut(s) 145, 975, 1286, 1338
MaeIII GTNAC 4 cut(s) 175, 965, 1282, 1429
MalI GATC 5 cut(s) 22, 228, 911, 1131, 1262
MboI GATC 5 cut(s) 20, 226, 909, 1129, 1260
MboII GAAGA 4 cut(s) 15, 734, 1109, 1124
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 5 cut(s) 343, 373, 856, 958, 1117
MluCI AATT 5 cut(s) 8, 97, 155, 1216, 1402
MmeI TCCRAC 2 cut(s) 1183, 1339
MseI TTAA 4 cut(s) 59, 399, 978, 1471
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 4 cut(s) 528, 832, 990, 1154
MunI CAATTG 1 cut(s) 8
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 4 cut(s) 528, 832, 990, 1154
MvnI CGCG 3 cut(s) 496, 692, 707
NdeII GATC 5 cut(s) 20, 226, 909, 1129, 1260
NheI GCTAGC 2 cut(s) 301, 874
NlaIII CATG 3 cut(s) 265, 629, 1201
NlaIV GGNNCC 4 cut(s) 341, 752, 1213, 1366
NmuCI GTSAC 3 cut(s) 965, 1282, 1429
NspV TTCGAA 1 cut(s) 135
PaqCI CACCTGC 1 cut(s) 533
PciSI GCTCTTC 1 cut(s) 1122
PcsI WCGNNNNNNNCGW 2 cut(s) 1104, 1365
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 1 cut(s) 191
PkrI GCNGC 9 cut(s) 556, 559, 568, 571, 742, 745, 1042, 1084, 1246
PpuMI RGGWCCY 1 cut(s) 1364
Psp1406I AACGTT 3 cut(s) 145, 975, 1338
Psp5II RGGWCCY 1 cut(s) 1364
Psp6I CCWGG 4 cut(s) 526, 830, 988, 1152
PspGI CCWGG 4 cut(s) 526, 830, 988, 1152
PspN4I GGNNCC 4 cut(s) 341, 752, 1213, 1366
PspOMI GGGCCC 1 cut(s) 339
PspPI GGNCC 8 cut(s) 339, 340, 381, 454, 812, 1212, 1364, 1459
PspPPI RGGWCCY 1 cut(s) 1364
PstI CTGCAG 2 cut(s) 559, 745
PstNI CAGNNNCTG 1 cut(s) 533
PsuI RGATCY 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 4 cut(s) 242, 428, 499, 920
RsaNI GTAC 4 cut(s) 241, 427, 498, 919
SapI GCTCTTC 1 cut(s) 1122
SaqAI TTAA 4 cut(s) 59, 399, 978, 1471
SatI GCNGC 9 cut(s) 555, 558, 567, 570, 741, 744, 1041, 1083, 1245
Sau3AI GATC 5 cut(s) 20, 226, 909, 1129, 1260
Sau96I GGNCC 8 cut(s) 339, 340, 381, 454, 812, 1212, 1364, 1459
ScrFI CCNGG 4 cut(s) 528, 832, 990, 1154
SduI GDGCHC 5 cut(s) 343, 373, 856, 958, 1117
SfaNI GCATC 5 cut(s) 205, 238, 335, 505, 519
SfcI CTRYAG 2 cut(s) 555, 741
SfuI TTCGAA 1 cut(s) 135
SinI GGWCC 4 cut(s) 454, 812, 1212, 1364
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 5 cut(s) 8, 97, 155, 1216, 1402
SspMI CTAG 3 cut(s) 302, 539, 875
StyD4I CCNGG 4 cut(s) 526, 830, 988, 1152
StyI CCWWGG 1 cut(s) 815
TaaI ACNGT 8 cut(s) 122, 209, 662, 758, 823, 953, 1103, 1429
TaiI ACGT 4 cut(s) 148, 978, 1289, 1341
TaqI TCGA 6 cut(s) 71, 135, 513, 1098, 1175, 1259
TasI AATT 5 cut(s) 8, 97, 155, 1216, 1402
TatI WGTACW 1 cut(s) 918
TauI GCSGC 3 cut(s) 569, 1043, 1085
TfiI GAWTC 1 cut(s) 191
Tru1I TTAA 4 cut(s) 59, 399, 978, 1471
Tru9I TTAA 4 cut(s) 59, 399, 978, 1471
TscAI CASTG 3 cut(s) 718, 907, 1287
TseFI GTSAC 3 cut(s) 965, 1282, 1429
TseI GCWGC 6 cut(s) 554, 557, 569, 740, 743, 1244
Tsp45I GTSAC 3 cut(s) 965, 1282, 1429
TspDTI ATGAA 3 cut(s) 278, 492, 735
TspGWI ACGGA 2 cut(s) 267, 444
TspRI CASTG 3 cut(s) 718, 907, 1287
VneI GTGCAC 1 cut(s) 852
VpaK11BI GGWCC 4 cut(s) 454, 812, 1212, 1364
XspI CTAG 3 cut(s) 302, 539, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.