RchiOBHm_Chr4g0446651

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003358
Physical Location & Seq
Reverse (-)
66763274 .. 66765011
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41416

Sequence Viewer

Length: 1194 bp
ATGATGATGTCTGTGCAGAGCAGTAGACGGGTCTTTGGTTTGGTTATTGGATCATTATCGATGCTTTTATTGTTCCAAGGAATTGCTTGTGAAGCAGACTATACTCAAGCACTTGCCAAGTCATTGTTGTATTATGAGGGTCAACGCTCAGGGAAGTTGCCCCCTATTGGCAAACTCCAGAGAGTGGCGTGGCGAGGAGATTCCGGACTAGAAGACGGCAGTGATGCTGGAATTGATTTGGTGGGAGGCTACTACGATGCCGGAGACAATCTCAAGTTGGGATTTCCAATGGCCTTCTCAGTAACAATGCTGTCATGGAGCACGATCGAATTCCAAAGCAAGCTCAAGGCCAAATATGAGATCGCTAATGCTCTTCGGGCTATCAAGTGGGGAACAGATTACTTGATAAAAGCACATCCACAGCCCGAAGTGCTTTATGGGCATATTGGGGGTGTTGATTCAGACCATCAATGTTGGCAGAGACCAGAGGACATGACCACCCCGAGGACCGTGTTCATGATTGATGACCAACACCCGGGATCTGATCTCGCCGCTGAAACTGCTGCTGCTTTGGCTGCTGCCTCAATTGCTTTTGAACGTTTTGATAGAAGTTACTCATACCAACTCGTAATTCATGCAAAGCAGCTATTTGATTTTGCACGCAATCATCCTGGTAAGTATCAAAACAGCATCCCTGAGGCAGGAAAAGTCTACGCCAGTAGTGGGTATCAGGACGAGATGCTGTGGGCAGCGGCATGGCTCCATCGTGCTACTAATGAACAGAAGTACCTCAACTTCCTAAGCGAAGCGGGAGATATAGGTGGAACAAGAACAACATTCTCATGGGATGACAAATTCGTCGGCGCACAGGTTTTGGCAGCACTGCTTGTAATGAAGGGAAAGGTTGATTCTTCAGGAAAATGGGGGGAGTATAAGCGCCATGCAGAGGAATTCATATGTTCATGCGTTCAGAAAGGAAACAACAACGTGAAGAAGACACCCGGAGGCTTGCTTTGGTTTCTGCCATGGAATAACAATCAATACGTTGCCACTGCTACATTTGTCACCACTGTTTATTCCGTTTATTTGGACGCAAAAAAGGCCTCCATCAACTGTCCTGGTGGCGCTGCTCACCCCTCTGATCTCATTGCATCTGCCAAGTCACAGGTATTTTATTTGATACATGAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

397

Amino Acids

43.86

Weight (kDa)

6.56

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 34 - 393 2.2e-118 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
AccB7I CCANNNNNTGG 1 cut(s) 184
AccI GTMKAC 2 cut(s) 25, 711
AccIII TCCGGA 1 cut(s) 203
AciI CCGC 3 cut(s) 552, 752, 809
AclI AACGTT 1 cut(s) 598
AclWI GGATC 2 cut(s) 58, 547
AcsI RAATTY 3 cut(s) 329, 854, 950
AcuI CTGAAG 1 cut(s) 897
AfaI GTAC 1 cut(s) 788
AfiI CCNNNNNNNGG 7 cut(s) 167, 184, 504, 535, 701, 723, 946
AgsI TTSAA 1 cut(s) 596
AjnI CCWGG 2 cut(s) 670, 1117
AjuI GAANNNNNNNTTGG 2 cut(s) 615, 647
AluBI AGCT 2 cut(s) 343, 646
AluI AGCT 2 cut(s) 343, 646
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 2 cut(s) 258, 475
AlwI GGATC 2 cut(s) 58, 547
Ama87I CYCGRG 2 cut(s) 502, 535
Aor13HI TCCGGA 1 cut(s) 203
AoxI GGCC 3 cut(s) 291, 348, 1101
ApeKI GCWGC 8 cut(s) 563, 566, 575, 578, 643, 749, 878, 1127
ApoI RAATTY 3 cut(s) 329, 854, 950
AspLEI GCGC 3 cut(s) 866, 939, 1127
AspS9I GGNCC 1 cut(s) 507
AsuC2I CCSGG 3 cut(s) 536, 537, 1002
AsuHPI GGTGA 2 cut(s) 1057, 1124
AvaI CYCGRG 2 cut(s) 502, 535
AvaII GGWCC 1 cut(s) 507
AxyI CCTNAGG 1 cut(s) 696
BaeI ACNNNNGTAYC 2 cut(s) 770, 803
BarI GAAGNNNNNNTAC 2 cut(s) 779, 811
BbsI GAAGAC 2 cut(s) 219, 1001
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 8 cut(s) 550, 553, 562, 565, 655, 761, 890, 1114
BccI CCATC 3 cut(s) 474, 771, 1115
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 672, 1119
BcnI CCSGG 3 cut(s) 536, 537, 1002
BcoDI GTCTC 2 cut(s) 258, 475
BfaI CTAG 1 cut(s) 209
BfoI RGCGCY 2 cut(s) 940, 1128
Bme1390I CCNGG 5 cut(s) 536, 537, 672, 1002, 1119
Bme18I GGWCC 1 cut(s) 507
BmeT110I CYCGRG 2 cut(s) 502, 535
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 1 cut(s) 761
BmrFI CCNGG 5 cut(s) 536, 537, 672, 1002, 1119
BmsI GCATC 6 cut(s) 51, 214, 247, 699, 729, 1160
BpiI GAAGAC 2 cut(s) 219, 1001
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 1 cut(s) 161
Bpu10I CCTNAGC 2 cut(s) 148, 800
BpuEI CTTGAG 3 cut(s) 90, 257, 329
BpuMI CCSGG 3 cut(s) 536, 537, 1002
Bsa29I ATCGAT 1 cut(s) 59
BsaBI GATNNNNATC 1 cut(s) 55
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 4 cut(s) 76, 503, 535, 1025
BsaWI WCCGGW 1 cut(s) 203
Bsc4I CCNNNNNNNGG 7 cut(s) 167, 184, 504, 535, 701, 723, 946
Bse1I ACTGG 1 cut(s) 717
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 1 cut(s) 1146
Bse8I GATNNNNATC 1 cut(s) 55
BseAI TCCGGA 1 cut(s) 203
BseBI CCWGG 2 cut(s) 672, 1119
BseCI ATCGAT 1 cut(s) 59
BseDI CCNNGG 4 cut(s) 76, 503, 535, 1025
BseGI GGATG 4 cut(s) 415, 667, 690, 853
BseJI GATNNNNATC 1 cut(s) 55
BseLI CCNNNNNNNGG 7 cut(s) 167, 184, 504, 535, 701, 723, 946
BseMI GCAATG 1 cut(s) 1146
BseMII CTCAG 3 cut(s) 162, 312, 687
BseNI ACTGG 1 cut(s) 717
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 8 cut(s) 550, 553, 562, 565, 655, 761, 890, 1114
BsgI GTGCAG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 3 cut(s) 293, 350, 1103
BshVI ATCGAT 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 323
BsiHKCI CYCGRG 2 cut(s) 502, 535
BsiSI CCGG 4 cut(s) 204, 261, 536, 1002
BslI CCNNNNNNNGG 7 cut(s) 167, 184, 504, 535, 701, 723, 946
BsmAI GTCTC 2 cut(s) 258, 475
BsnI GGCC 3 cut(s) 293, 350, 1103
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 2 cut(s) 502, 535
Bsp1286I GDGCHC 1 cut(s) 323
Bsp13I TCCGGA 1 cut(s) 203
Bsp143I GATC 6 cut(s) 50, 324, 360, 539, 544, 1141
Bsp19I CCATGG 1 cut(s) 1025
BspACI CCGC 3 cut(s) 552, 752, 809
BspANI GGCC 3 cut(s) 293, 350, 1103
BspCNI CTCAG 3 cut(s) 161, 311, 688
BspDI ATCGAT 1 cut(s) 59
BspEI TCCGGA 1 cut(s) 203
BspHI TCATGA 1 cut(s) 516
BspLI GGNNCC 1 cut(s) 761
BspPI GGATC 2 cut(s) 58, 547
BspQI GCTCTTC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 1146
BsrI ACTGG 1 cut(s) 717
BssECI CCNNGG 4 cut(s) 76, 503, 535, 1025
BssMI GATC 6 cut(s) 50, 324, 360, 539, 544, 1141
BssT1I CCWWGG 2 cut(s) 76, 1025
Bst2UI CCWGG 2 cut(s) 672, 1119
Bst4CI ACNGT 3 cut(s) 511, 1072, 1115
Bst6I CTCTTC 1 cut(s) 378
BstC8I GCNNGC 3 cut(s) 341, 661, 1010
BstDEI CTNAG 4 cut(s) 148, 298, 696, 800
BstDSI CCRYGG 1 cut(s) 1025
BstENI CCTNNNNNAGG 1 cut(s) 699
BstF5I GGATG 4 cut(s) 415, 667, 690, 853
BstH2I RGCGCY 2 cut(s) 940, 1128
BstHHI GCGC 3 cut(s) 866, 939, 1127
BstKTI GATC 6 cut(s) 53, 327, 363, 542, 547, 1144
BstMAI GTCTC 2 cut(s) 258, 475
BstMBI GATC 6 cut(s) 50, 324, 360, 539, 544, 1141
BstMCI CGRYCG 1 cut(s) 327
BstMWI GCNNNNNNNGC 9 cut(s) 92, 377, 430, 439, 560, 572, 575, 587, 1100
BstNI CCWGG 2 cut(s) 672, 1119
BstSCI CCNGG 5 cut(s) 534, 535, 670, 1000, 1117
BstV1I GCAGC 8 cut(s) 550, 553, 562, 565, 655, 761, 890, 1114
BstV2I GAAGAC 2 cut(s) 219, 1001
BstX2I RGATCY 1 cut(s) 539
BstYI RGATCY 1 cut(s) 539
Bsu15I ATCGAT 1 cut(s) 59
Bsu36I CCTNAGG 1 cut(s) 696
BsuRI GGCC 3 cut(s) 293, 350, 1103
BsuTUI ATCGAT 1 cut(s) 59
BtgI CCRYGG 1 cut(s) 1025
BtsCI GGATG 4 cut(s) 415, 667, 690, 853
BtsI GCAGTG 3 cut(s) 226, 881, 1050
BtsIMutI CAGTG 4 cut(s) 226, 881, 1050, 1068
Cac8I GCNNGC 3 cut(s) 341, 661, 1010
CciI TCATGA 1 cut(s) 516
CfoI GCGC 3 cut(s) 866, 939, 1127
Cfr13I GGNCC 1 cut(s) 507
Cfr9I CCCGGG 1 cut(s) 535
ClaI ATCGAT 1 cut(s) 59
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 1 cut(s) 787
CviQI GTAC 1 cut(s) 787
DdeI CTNAG 4 cut(s) 148, 298, 696, 800
DpnI GATC 6 cut(s) 52, 326, 362, 541, 546, 1143
DpnII GATC 6 cut(s) 50, 324, 360, 539, 544, 1141
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco130I CCWWGG 2 cut(s) 76, 1025
Eco147I AGGCCT 1 cut(s) 1103
Eco31I GGTCTC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 507
Eco57I CTGAAG 1 cut(s) 897
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 2 cut(s) 502, 535
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoRI GAATTC 2 cut(s) 329, 950
EcoRII CCWGG 2 cut(s) 670, 1117
EcoT14I CCWWGG 2 cut(s) 76, 1025
ErhI CCWWGG 2 cut(s) 76, 1025
FauI CCCGC 1 cut(s) 802
FauNDI CATATG 1 cut(s) 956
FblI GTMKAC 2 cut(s) 25, 711
FokI GGATG 4 cut(s) 402, 654, 677, 860
FspBI CTAG 1 cut(s) 209
GlaI GCGC 3 cut(s) 865, 938, 1126
GsuI CTGGAG 1 cut(s) 161
HaeII RGCGCY 2 cut(s) 940, 1128
HaeIII GGCC 3 cut(s) 293, 350, 1103
HapII CCGG 4 cut(s) 204, 261, 536, 1002
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 3 cut(s) 866, 939, 1127
Hin6I GCGC 3 cut(s) 864, 937, 1125
HinP1I GCGC 3 cut(s) 864, 937, 1125
HincII GTYRAC 1 cut(s) 143
HindII GTYRAC 1 cut(s) 143
HinfI GANTC 3 cut(s) 200, 458, 908
HpaII CCGG 4 cut(s) 204, 261, 536, 1002
HphI GGTGA 2 cut(s) 1057, 1124
Hpy166II GTNNAC 3 cut(s) 26, 143, 712
Hpy188I TCNGA 4 cut(s) 463, 544, 972, 1141
Hpy188III TCNNGA 5 cut(s) 178, 204, 517, 731, 915
Hpy8I GTNNAC 3 cut(s) 26, 143, 712
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 2 cut(s) 304, 889
HpyCH4III ACNGT 3 cut(s) 511, 1072, 1115
HpyCH4IV ACGT 3 cut(s) 598, 987, 1044
HpyCH4V TGCA 5 cut(s) 16, 638, 659, 944, 1151
HpyF10VI GCNNNNNNNGC 9 cut(s) 92, 377, 430, 439, 560, 572, 575, 587, 1100
HpyF3I CTNAG 4 cut(s) 148, 298, 696, 800
HpySE526I ACGT 3 cut(s) 598, 987, 1044
HspAI GCGC 3 cut(s) 864, 937, 1125
Kpn2I TCCGGA 1 cut(s) 203
Kzo9I GATC 6 cut(s) 50, 324, 360, 539, 544, 1141
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 2 cut(s) 318, 765
Lsp1109I GCAGC 8 cut(s) 550, 553, 562, 565, 655, 761, 890, 1114
LweI GCATC 6 cut(s) 51, 214, 247, 699, 729, 1160
MaeI CTAG 1 cut(s) 209
MaeII ACGT 3 cut(s) 598, 987, 1044
MaeIII GTNAC 4 cut(s) 301, 611, 1063, 1161
MalI GATC 6 cut(s) 52, 326, 362, 541, 546, 1143
MboI GATC 6 cut(s) 50, 324, 360, 539, 544, 1141
MboII GAAGA 5 cut(s) 224, 365, 903, 1003, 1006
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 7 cut(s) 81, 231, 329, 585, 630, 854, 950
MroI TCCGGA 1 cut(s) 203
MseI TTAA 1 cut(s) 1192
MslI CAYNNNNRTG 1 cut(s) 841
MspA1I CMGCKG 2 cut(s) 554, 752
MspI CCGG 4 cut(s) 204, 261, 536, 1002
MspR9I CCNGG 5 cut(s) 536, 537, 672, 1002, 1119
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 2 cut(s) 672, 1119
MwoI GCNNNNNNNGC 9 cut(s) 92, 377, 430, 439, 560, 572, 575, 587, 1100
NciI CCSGG 3 cut(s) 536, 537, 1002
NcoI CCATGG 1 cut(s) 1025
NdeI CATATG 1 cut(s) 956
NdeII GATC 6 cut(s) 50, 324, 360, 539, 544, 1141
NlaIV GGNNCC 1 cut(s) 761
NmuCI GTSAC 2 cut(s) 1063, 1161
PagI TCATGA 1 cut(s) 516
PceI AGGCCT 1 cut(s) 1103
PciSI GCTCTTC 1 cut(s) 378
PfeI GAWTC 3 cut(s) 200, 458, 908
PflMI CCANNNNNTGG 1 cut(s) 184
Ple19I CGATCG 1 cut(s) 327
Psp1406I AACGTT 1 cut(s) 598
Psp6I CCWGG 2 cut(s) 670, 1117
PspGI CCWGG 2 cut(s) 670, 1117
PspN4I GGNNCC 1 cut(s) 761
PspPI GGNCC 1 cut(s) 507
PsuI RGATCY 1 cut(s) 539
PvuI CGATCG 1 cut(s) 327
RsaI GTAC 1 cut(s) 788
RsaNI GTAC 1 cut(s) 787
RseI CAYNNNNRTG 1 cut(s) 841
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 1 cut(s) 1192
Sau3AI GATC 6 cut(s) 50, 324, 360, 539, 544, 1141
Sau96I GGNCC 1 cut(s) 507
ScrFI CCNGG 5 cut(s) 536, 537, 672, 1002, 1119
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 6 cut(s) 51, 214, 247, 699, 729, 1160
SinI GGWCC 1 cut(s) 507
SmaI CCCGGG 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 841
SmlI CTYRAG 3 cut(s) 105, 272, 344
SmoI CTYRAG 3 cut(s) 105, 272, 344
Sse9I AATT 7 cut(s) 81, 231, 329, 585, 630, 854, 950
SseBI AGGCCT 1 cut(s) 1103
SsiI CCGC 3 cut(s) 552, 752, 809
SspMI CTAG 1 cut(s) 209
StuI AGGCCT 1 cut(s) 1103
StyD4I CCNGG 5 cut(s) 534, 535, 670, 1000, 1117
StyI CCWWGG 2 cut(s) 76, 1025
TaaI ACNGT 3 cut(s) 511, 1072, 1115
TaiI ACGT 3 cut(s) 601, 990, 1047
TaqI TCGA 2 cut(s) 59, 327
TasI AATT 7 cut(s) 81, 231, 329, 585, 630, 854, 950
TauI GCSGC 2 cut(s) 554, 755
TfiI GAWTC 3 cut(s) 200, 458, 908
Tru1I TTAA 1 cut(s) 1192
Tru9I TTAA 1 cut(s) 1192
TscAI CASTG 4 cut(s) 226, 888, 1057, 1075
TseFI GTSAC 2 cut(s) 1063, 1161
TseI GCWGC 8 cut(s) 563, 566, 575, 578, 643, 749, 878, 1127
Tsp45I GTSAC 2 cut(s) 1063, 1161
TspDTI ATGAA 6 cut(s) 505, 623, 792, 908, 943, 951
TspGWI ACGGA 1 cut(s) 1069
TspMI CCCGGG 1 cut(s) 535
TspRI CASTG 4 cut(s) 226, 888, 1057, 1075
Van91I CCANNNNNTGG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 507
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 3 cut(s) 329, 854, 950
XmaI CCCGGG 1 cut(s) 535
XmiI GTMKAC 2 cut(s) 25, 711
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.