AT2G44570

Contains the following InterPro domains Six-hairpin glycosidase (InterPro IPR012341), Glycoside hydrolase, family 9, active site (InterPro IPR018221), Six-hairpin glycosidase-like (InterPro IPR008928), Glycoside hydrolase, family 9 (InterPro IPR001701)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003358
Physical Location & Seq
Reverse (-)
18394196 .. 18396337
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44570.1

Sequence Viewer

Length: 1479 bp
ATGTCTTGTATATCATCACAATGCTTCATCACAATCAAAAGCATTTGCATTGTGTTATTATTATCGATTACTTGTGGAGCAGTTTCTGCAAATTCAAATTATGGAGAAGCTCTTACGAAGAGTCTTTTGTATTTCGAAGCTCAACGCTCCGGAAAATTGCCTTTGGACCAAAGAGTTATATGGAGAGGAGATTCTGCACTCAGAGATGGTTCTGATGCTCATGTTGATCTCACCGGAGGGTATTACGATGCTGGAGATAATATGAAGTTCGGATTTCCTTTAGCGTTCACAACAACAATGCTAGCTTGGAGTAGTGTAGAGATGGAATCACAGCTTAAGGCCCATCAGGAGCACGGAAACACCCTCGCGGCTCTCAGGTGGGCCACCGATTATCTCATTAAAGCCCATCCTGAGCCCAATGTCTTGTACGGACAGGTGGGTGATGCCAACTTGGACCATGCCTGCTGGATGAGACCCGAGGATATGACCACTCCGCGCCCTTCATACCGCATTGACCCTCAGCATCCTGGTGCGGATCTCGCGGGTGAGACAGCAGCAGCGATGGCTGCGGCTTCTTTGGCATTTGCGCCATCTGATTCAGCCTACGCCAAAACACTTATTAGTCACGCAAAAGAACTATTTGAATTCGCTAAGGATTACCCTGGAGTCTATCATAACTCCATTCCTAACGCGGGTGGCTTTTACCCTAGCAGCGGTTACGAAGATGAGTTACTTTGGGCCGCGGCTTGGCTTCACCGTGCCACTGGAGACCAGACTTATCTCGATCATCTAACACAAGCTTCTAATAGTGGAGGTGCAAGGTCCGTTTTTGCGTGGGACGATAAGTTCTTGGGAGCGCAAGTCTTGGTAGCCAAGCTTGTGTTTGAAGGGAAAGTGAAGAACGAAGGGAAGATGATTGAGTACAAGAGTATGGCAGAGCAATTCATCTGCAACTGTGCTCAAAAGGGTTTCAACAATGTTAAAAAGACCCCAGGAGGTTTGCTCTGGTTCTTGCCGTGGGACAACCTCCAGTACACTGCCACCGCTTCTTTCGCATTAGCCACTTATGCAAAATACCTCGAAGCTGCACAAACATCGATTCAATGCCCCAATGGTGATGTCCTTCAAGCTTCTGATCTTCTTAACCTTGCTCGTGCCCAGGTAGACTACATACTTGGGTCAAACCCCAAGAAAATGAGCTACATGGTTGGATACGGGACCAATTATCCTAAAAGACCACACCATAGAGGAGCCTCTATAGTGTCAATCAAAAATGATCCTAAACCGGTGACATGTAATGGAGGGTTTGAAGCTTGGTACAACAATCCAAAACCGAATCCTAATCTTTTGGTTGGAGCAATTGTAGGTGGACCGGACGAGTACGATGCATACGGAGATGAACGATCTGATTTTCAGCACGGCGAGCCTGATACCGTCACGGTTGCTCCTTTGCTTGGTGTTCTGGCTGCCATTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

492

Amino Acids

53.59

Weight (kDa)

5.54

Isoelectric Point (pI)

28.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 34 - 488 3.1e-160 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1164
AccII CGCG 5 cut(s) 368, 496, 542, 692, 743
AccIII TCCGGA 1 cut(s) 149
AclWI GGATC 2 cut(s) 543, 1271
AcsI RAATTY 2 cut(s) 91, 644
AfaI GTAC 5 cut(s) 428, 923, 1034, 1319, 1382
AfiI CCNNNNNNNGG 4 cut(s) 692, 713, 747, 1454
AflII CTTAAG 1 cut(s) 335
AflIII ACRYGT 1 cut(s) 1292
AgeI ACCGGT 1 cut(s) 1285
AgsI TTSAA 7 cut(s) 96, 644, 887, 973, 1103, 1127, 1310
AjnI CCWGG 4 cut(s) 526, 661, 991, 1158
AloI GAACNNNNNNTCC 2 cut(s) 830, 862
Alw21I GWGCWC 2 cut(s) 354, 961
Alw26I GTCTC 3 cut(s) 466, 542, 762
AlwI GGATC 2 cut(s) 543, 1271
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 476
Aor13HI TCCGGA 1 cut(s) 149
AoxI GGCC 3 cut(s) 339, 381, 738
ApeKI GCWGC 6 cut(s) 554, 557, 566, 711, 1085, 1466
ApoI RAATTY 2 cut(s) 91, 644
AsiGI ACCGGT 1 cut(s) 1285
AspLEI GCGC 3 cut(s) 498, 589, 859
AspS9I GGNCC 8 cut(s) 166, 340, 381, 454, 738, 822, 1218, 1370
AsuHPI GGTGA 6 cut(s) 223, 452, 557, 746, 1127, 1300
AsuII TTCGAA 1 cut(s) 135
AsuNHI GCTAGC 1 cut(s) 301
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 5 cut(s) 166, 454, 822, 1218, 1370
BaeGI GKGCMC 1 cut(s) 1159
BanII GRGCYC 1 cut(s) 417
BarI GAAGNNNNNNTAC 2 cut(s) 714, 746
BauI CACGAG 1 cut(s) 1152
Bbv12I GWGCWC 2 cut(s) 354, 961
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 6 cut(s) 553, 566, 569, 723, 1072, 1453
BccI CCATC 6 cut(s) 200, 316, 351, 414, 556, 598
BceAI ACGGC 2 cut(s) 1000, 1435
BcgI CGANNNNNNTGC 4 cut(s) 1077, 1111, 1367, 1401
BciT130I CCWGG 4 cut(s) 528, 663, 993, 1160
BciVI GTATCC 1 cut(s) 1205
BcoDI GTCTC 3 cut(s) 466, 542, 762
BfaI CTAG 2 cut(s) 302, 708
BfmI CTRYAG 1 cut(s) 1257
BfrI CTTAAG 1 cut(s) 335
BfuI GTATCC 1 cut(s) 1205
Bme1390I CCNGG 4 cut(s) 528, 663, 993, 1160
Bme18I GGWCC 5 cut(s) 166, 454, 822, 1218, 1370
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 8 cut(s) 166, 340, 381, 454, 738, 822, 1218, 1370
BmiI GGNNCC 2 cut(s) 1219, 1252
BmrFI CCNGG 4 cut(s) 528, 663, 993, 1160
BmsI GCATC 5 cut(s) 205, 238, 433, 532, 1375
BmtI GCTAGC 1 cut(s) 305
BplI GAGNNNNNCTC 2 cut(s) 987, 1019
BpmI CTGGAG 4 cut(s) 273, 684, 786, 1013
Bpu10I CCTNAGC 3 cut(s) 411, 519, 651
Bpu14I TTCGAA 1 cut(s) 135
Bsa29I ATCGAT 2 cut(s) 65, 1097
BsaI GGTCTC 2 cut(s) 466, 762
BsaJI CCNNGG 6 cut(s) 477, 661, 741, 991, 1016, 1158
BsaWI WCCGGW 4 cut(s) 149, 233, 1285, 1372
BsaXI ACNNNNNCTCC 6 cut(s) 846, 876, 1347, 1377, 1429, 1459
Bsc4I CCNNNNNNNGG 4 cut(s) 692, 713, 747, 1454
Bse118I RCCGGY 1 cut(s) 1285
Bse1I ACTGG 2 cut(s) 769, 1030
Bse3DI GCAATG 1 cut(s) 1470
BseAI TCCGGA 1 cut(s) 149
BseBI CCWGG 4 cut(s) 528, 663, 993, 1160
BseCI ATCGAT 2 cut(s) 65, 1097
BseDI CCNNGG 6 cut(s) 477, 661, 741, 991, 1016, 1158
BseGI GGATG 3 cut(s) 406, 474, 523
BseLI CCNNNNNNNGG 4 cut(s) 692, 713, 747, 1454
BseMI GCAATG 1 cut(s) 1470
BseMII CTCAG 4 cut(s) 214, 388, 402, 533
BseNI ACTGG 2 cut(s) 769, 1030
BseRI GAGGAG 2 cut(s) 201, 1263
BseSI GKGCMC 1 cut(s) 1159
BseXI GCAGC 6 cut(s) 553, 566, 569, 723, 1072, 1453
BsgI GTGCAG 2 cut(s) 180, 1071
Bsh1236I CGCG 5 cut(s) 368, 496, 542, 692, 743
BshFI GGCC 3 cut(s) 341, 383, 740
BshTI ACCGGT 1 cut(s) 1285
BshVI ATCGAT 2 cut(s) 65, 1097
BsiHKAI GWGCWC 2 cut(s) 354, 961
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 4 cut(s) 150, 234, 1286, 1373
BslFI GGGAC 3 cut(s) 851, 1034, 1231
BslI CCNNNNNNNGG 4 cut(s) 692, 713, 747, 1454
BsmAI GTCTC 3 cut(s) 466, 542, 762
BsmFI GGGAC 3 cut(s) 851, 1034, 1231
BsnI GGCC 3 cut(s) 341, 383, 740
Bso31I GGTCTC 2 cut(s) 466, 762
BsoBI CYCGRG 1 cut(s) 476
Bsp119I TTCGAA 1 cut(s) 135
Bsp1286I GDGCHC 4 cut(s) 354, 417, 961, 1159
Bsp13I TCCGGA 1 cut(s) 149
Bsp143I GATC 6 cut(s) 226, 535, 784, 1135, 1276, 1403
BspANI GGCC 3 cut(s) 341, 383, 740
BspCNI CTCAG 4 cut(s) 213, 387, 403, 532
BspDI ATCGAT 2 cut(s) 65, 1097
BspEI TCCGGA 1 cut(s) 149
BspFNI CGCG 5 cut(s) 368, 496, 542, 692, 743
BspLI GGNNCC 2 cut(s) 1219, 1252
BspOI GCTAGC 1 cut(s) 305
BspPI GGATC 2 cut(s) 543, 1271
BspT104I TTCGAA 1 cut(s) 135
BspTI CTTAAG 1 cut(s) 335
BspTNI GGTCTC 2 cut(s) 466, 762
BsrDI GCAATG 1 cut(s) 1470
BsrFI RCCGGY 1 cut(s) 1285
BsrI ACTGG 2 cut(s) 769, 1030
BssAI RCCGGY 1 cut(s) 1285
BssECI CCNNGG 6 cut(s) 477, 661, 741, 991, 1016, 1158
BssMI GATC 6 cut(s) 226, 535, 784, 1135, 1276, 1403
BssSI CACGAG 1 cut(s) 1152
Bst2BI CACGAG 1 cut(s) 1152
Bst2UI CCWGG 4 cut(s) 528, 663, 993, 1160
Bst4CI ACNGT 4 cut(s) 758, 956, 1435, 1441
Bst6I CTCTTC 1 cut(s) 113
BstAFI CTTAAG 1 cut(s) 335
BstAPI GCANNNNNTGC 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 135
BstC8I GCNNGC 3 cut(s) 303, 463, 1424
BstDEI CTNAG 5 cut(s) 200, 374, 411, 519, 651
BstDSI CCRYGG 2 cut(s) 741, 1016
BstF5I GGATG 3 cut(s) 406, 474, 523
BstFNI CGCG 5 cut(s) 368, 496, 542, 692, 743
BstHHI GCGC 3 cut(s) 498, 589, 859
BstKTI GATC 6 cut(s) 229, 538, 787, 1138, 1279, 1406
BstMAI GTCTC 3 cut(s) 466, 542, 762
BstMBI GATC 6 cut(s) 226, 535, 784, 1135, 1276, 1403
BstMWI GCNNNNNNNGC 9 cut(s) 86, 539, 563, 566, 578, 1052, 1067, 1423, 1472
BstNI CCWGG 4 cut(s) 528, 663, 993, 1160
BstNSI RCATGY 1 cut(s) 1296
BstSCI CCNGG 4 cut(s) 526, 661, 991, 1158
BstSFI CTRYAG 1 cut(s) 1257
BstSLI GKGCMC 1 cut(s) 1159
BstUI CGCG 5 cut(s) 368, 496, 542, 692, 743
BstV1I GCAGC 6 cut(s) 553, 566, 569, 723, 1072, 1453
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
Bsu15I ATCGAT 2 cut(s) 65, 1097
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 3 cut(s) 341, 383, 740
BsuTUI ATCGAT 2 cut(s) 65, 1097
BtgI CCRYGG 2 cut(s) 741, 1016
BtgZI GCGATG 1 cut(s) 575
BtsCI GGATG 3 cut(s) 406, 474, 523
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 2 cut(s) 762, 1035
Cac8I GCNNGC 3 cut(s) 303, 463, 1424
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 3 cut(s) 498, 589, 859
Cfr10I RCCGGY 1 cut(s) 1285
Cfr13I GGNCC 8 cut(s) 166, 340, 381, 454, 738, 822, 1218, 1370
Cfr42I CCGCGG 1 cut(s) 744
ClaI ATCGAT 2 cut(s) 65, 1097
Csp6I GTAC 5 cut(s) 427, 922, 1033, 1318, 1381
CspAI ACCGGT 1 cut(s) 1285
CviAII CATG 5 cut(s) 221, 458, 1204, 1293, 1476
CviQI GTAC 5 cut(s) 427, 922, 1033, 1318, 1381
DdeI CTNAG 5 cut(s) 200, 374, 411, 519, 651
DpnI GATC 6 cut(s) 228, 537, 786, 1137, 1278, 1405
DpnII GATC 6 cut(s) 226, 535, 784, 1135, 1276, 1403
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 417
Eco31I GGTCTC 2 cut(s) 466, 762
Eco47I GGWCC 5 cut(s) 166, 454, 822, 1218, 1370
Eco88I CYCGRG 1 cut(s) 476
EcoRI GAATTC 1 cut(s) 644
EcoRII CCWGG 4 cut(s) 526, 661, 991, 1158
EcoT22I ATGCAT 1 cut(s) 1390
EcoT38I GRGCYC 1 cut(s) 417
FaeI CATG 5 cut(s) 224, 461, 1207, 1296, 1479
FaqI GGGAC 3 cut(s) 851, 1034, 1231
FatI CATG 5 cut(s) 220, 457, 1203, 1292, 1475
FauI CCCGC 2 cut(s) 535, 685
FblI GTMKAC 1 cut(s) 1164
FokI GGATG 3 cut(s) 393, 481, 510
FriOI GRGCYC 1 cut(s) 417
FspBI CTAG 2 cut(s) 302, 708
GlaI GCGC 3 cut(s) 497, 588, 858
GsuI CTGGAG 4 cut(s) 273, 684, 786, 1013
HaeIII GGCC 3 cut(s) 341, 383, 740
HapII CCGG 4 cut(s) 150, 234, 1286, 1373
HhaI GCGC 3 cut(s) 498, 589, 859
Hin1II CATG 5 cut(s) 224, 461, 1207, 1296, 1479
Hin6I GCGC 3 cut(s) 496, 587, 857
HinP1I GCGC 3 cut(s) 496, 587, 857
HindIII AAGCTT 4 cut(s) 798, 875, 1128, 1311
HinfI GANTC 7 cut(s) 121, 191, 326, 596, 666, 1099, 1336
HpaII CCGG 4 cut(s) 150, 234, 1286, 1373
HphI GGTGA 6 cut(s) 223, 452, 557, 746, 1127, 1300
Hpy166II GTNNAC 4 cut(s) 288, 1035, 1165, 1370
Hpy188I TCNGA 6 cut(s) 203, 214, 272, 595, 1135, 1408
Hpy188III TCNNGA 4 cut(s) 150, 347, 410, 782
Hpy8I GTNNAC 4 cut(s) 288, 1035, 1165, 1370
HpyAV CCTTC 4 cut(s) 510, 881, 899, 1133
HpyCH4III ACNGT 4 cut(s) 758, 956, 1435, 1441
HpyCH4V TGCA 9 cut(s) 48, 89, 197, 818, 951, 1070, 1088, 1388, 1475
HpyF10VI GCNNNNNNNGC 9 cut(s) 86, 539, 563, 566, 578, 1052, 1067, 1423, 1472
HpyF3I CTNAG 5 cut(s) 200, 374, 411, 519, 651
Hsp92II CATG 5 cut(s) 224, 461, 1207, 1296, 1479
HspAI GCGC 3 cut(s) 496, 587, 857
Kpn2I TCCGGA 1 cut(s) 149
KspI CCGCGG 1 cut(s) 744
Kzo9I GATC 6 cut(s) 226, 535, 784, 1135, 1276, 1403
LmnI GCTCC 7 cut(s) 77, 152, 349, 854, 1250, 1355, 1450
Lsp1109I GCAGC 6 cut(s) 553, 566, 569, 723, 1072, 1453
LweI GCATC 5 cut(s) 205, 238, 433, 532, 1375
MaeI CTAG 2 cut(s) 302, 708
MaeIII GTNAC 5 cut(s) 623, 716, 729, 1288, 1435
MalI GATC 6 cut(s) 228, 537, 786, 1137, 1278, 1405
MboI GATC 6 cut(s) 226, 535, 784, 1135, 1276, 1403
MboII GAAGA 5 cut(s) 130, 734, 910, 922, 1130
MfeI CAATTG 1 cut(s) 1359
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 4 cut(s) 354, 417, 961, 1159
MluCI AATT 7 cut(s) 91, 97, 155, 644, 941, 1222, 1359
MlyI GAGTC 2 cut(s) 130, 675
MmeI TCCRAC 2 cut(s) 1189, 1333
Mph1103I ATGCAT 1 cut(s) 1390
MroI TCCGGA 1 cut(s) 149
MseI TTAA 4 cut(s) 336, 399, 981, 1143
MslI CAYNNNNRTG 2 cut(s) 19, 528
MspA1I CMGCKG 2 cut(s) 714, 743
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 4 cut(s) 150, 234, 1286, 1373
MspR9I CCNGG 4 cut(s) 528, 663, 993, 1160
MunI CAATTG 1 cut(s) 1359
MvaI CCWGG 4 cut(s) 528, 663, 993, 1160
MvnI CGCG 5 cut(s) 368, 496, 542, 692, 743
MwoI GCNNNNNNNGC 9 cut(s) 86, 539, 563, 566, 578, 1052, 1067, 1423, 1472
NdeII GATC 6 cut(s) 226, 535, 784, 1135, 1276, 1403
NheI GCTAGC 1 cut(s) 301
NlaIII CATG 5 cut(s) 224, 461, 1207, 1296, 1479
NlaIV GGNNCC 2 cut(s) 1219, 1252
NmuCI GTSAC 3 cut(s) 623, 1288, 1435
NsiI ATGCAT 1 cut(s) 1390
NspI RCATGY 1 cut(s) 1296
NspV TTCGAA 1 cut(s) 135
PciI ACATGT 1 cut(s) 1292
PfeI GAWTC 5 cut(s) 191, 326, 596, 1099, 1336
PinAI ACCGGT 1 cut(s) 1285
PleI GAGTC 2 cut(s) 129, 674
PpsI GAGTC 2 cut(s) 129, 674
PscI ACATGT 1 cut(s) 1292
Psp6I CCWGG 4 cut(s) 526, 661, 991, 1158
PspGI CCWGG 4 cut(s) 526, 661, 991, 1158
PspN4I GGNNCC 2 cut(s) 1219, 1252
PspPI GGNCC 8 cut(s) 166, 340, 381, 454, 738, 822, 1218, 1370
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 5 cut(s) 428, 923, 1034, 1319, 1382
RsaNI GTAC 5 cut(s) 427, 922, 1033, 1318, 1381
RseI CAYNNNNRTG 2 cut(s) 19, 528
SacII CCGCGG 1 cut(s) 744
SaqAI TTAA 4 cut(s) 336, 399, 981, 1143
Sau3AI GATC 6 cut(s) 226, 535, 784, 1135, 1276, 1403
Sau96I GGNCC 8 cut(s) 166, 340, 381, 454, 738, 822, 1218, 1370
SchI GAGTC 2 cut(s) 130, 675
ScrFI CCNGG 4 cut(s) 528, 663, 993, 1160
SduI GDGCHC 4 cut(s) 354, 417, 961, 1159
SfaNI GCATC 5 cut(s) 205, 238, 433, 532, 1375
SfcI CTRYAG 1 cut(s) 1257
Sfr303I CCGCGG 1 cut(s) 744
SfuI TTCGAA 1 cut(s) 135
SgrBI CCGCGG 1 cut(s) 744
SinI GGWCC 5 cut(s) 166, 454, 822, 1218, 1370
SmiMI CAYNNNNRTG 2 cut(s) 19, 528
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 7 cut(s) 91, 97, 155, 644, 941, 1222, 1359
SspMI CTAG 2 cut(s) 302, 708
StyD4I CCNGG 4 cut(s) 526, 661, 991, 1158
TaaI ACNGT 4 cut(s) 758, 956, 1435, 1441
TaqI TCGA 5 cut(s) 65, 135, 783, 1080, 1097
TasI AATT 7 cut(s) 91, 97, 155, 644, 941, 1222, 1359
TatI WGTACW 2 cut(s) 921, 1032
TauI GCSGC 4 cut(s) 371, 572, 743, 746
TfiI GAWTC 5 cut(s) 191, 326, 596, 1099, 1336
Tru1I TTAA 4 cut(s) 336, 399, 981, 1143
Tru9I TTAA 4 cut(s) 336, 399, 981, 1143
TscAI CASTG 2 cut(s) 769, 1042
TseFI GTSAC 3 cut(s) 623, 1288, 1435
TseI GCWGC 6 cut(s) 554, 557, 566, 711, 1085, 1466
Tsp45I GTSAC 3 cut(s) 623, 1288, 1435
TspDTI ATGAA 5 cut(s) 16, 278, 492, 934, 1413
TspGWI ACGGA 4 cut(s) 369, 444, 814, 1407
TspRI CASTG 2 cut(s) 769, 1042
Vha464I CTTAAG 1 cut(s) 335
VpaK11BI GGWCC 5 cut(s) 166, 454, 822, 1218, 1370
XapI RAATTY 2 cut(s) 91, 644
XceI RCATGY 1 cut(s) 1296
XmiI GTMKAC 1 cut(s) 1164
XspI CTAG 2 cut(s) 302, 708
Zsp2I ATGCAT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.