FvH4_4g36880

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003358
Physical Location & Seq
Reverse (-)
33621014 .. 33623147
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36880.t2

Sequence Viewer

Length: 1476 bp
ATGGTGTCTGTGCAGAGCAGTAGAGGGGTCTTCGGTTTGGTTATTGGATCATTAGCAATGTTTCTATTGTTCCAAGGAATTGCTTGTCATGCAGACTATACACAAGCACTTACCAAGTCATTGTTGTATTATGAGGGTCAACGCTCAGGAAAGTTGACTCCTAATGGCAAACTCCAAAGAGTGGCTTGGCGAGGAGATTCTGGACTCAACGATGGCAGTGATGCTGGAATTGATTTGGTGGGAGGCTACTACGATGCCGGAGACAATCTCAAGCTGGGATTTCCGATGGCCTTCACAGTAACAATGCTTTCATGGAGCACAATCGAGTTCCACGGCAAGCTCAGGGAAAAATATGAGCTTCCAAATGCTCTTCGTGCTATCAAGTGGGGAACAGATTACTTGATAAAAGCACATCCACAGCCTGAAGTGCTGTATGGCCAAGTTGGGGGTGTTGATTCGGACCATCAATGTTGGCAGAGACCAGAGGACATGACCACCTCAAGGAATGTCTTCAGGATTGATGACCAACACCCGGGCTCTGATCTCGCCGCTGAAACTGCCGCTGCTTTGGCTGCTGCCTCGATTGCTATAGGTCGTTTCGATAGAAGTTACTCATCCCTACTTGTAACTCATGCAAAGCAGCTCTTTGATTTTGCTCGCAATCATCCCGGTGCTTATCAAGACAGCATCCCTGAGGCAGGGGAAGTCTACGCCAGTAGTGGGTATCAGGACGAGATGCTGTGGGCAGCAGCATGGCTCCATCGTGCTACTAATGAAGGGCAGTACCTCGAGTTCCTAAGTGAAGCCGGAGATACAGGTGGAACAAGAACAACATTCTCATGGGATGACAAATTTGTCGGTGCACAGGTTTTGGCTGCAATGCTTGTAATGAAGGGAAAAGTTGATCCTACGGGGAAATGGGGGGAGTATACGAGCTATGCAGAGAATTTCATATGTTCGTGCATTCAGAAAGGAAACAACAACGTGCAGAAGACACCCGCAGGCTTGCTTTGGTTTCTGCCATGGAATAACAATCAATATGTTGCCACTGCTTCATTTGTAACTAGTGTTTATTCCGTTTATATGGAGGCTAATAAGGCCTCCATCAACTGTCCTGGTGGCGTTGCTCACCCCTCTGATCTCATAGCATTCGCCAAATCACAGGTGGATTACATCTTGGGTTCAAACCCAAAAAACATGAGCTACATGGTTGGATATGGATCGAATTATCCTAAGCAAATTCATCACAGAGCAGCATCAATCGTCTCAATCAAGACGGATCCAGCTCCGGTGACATGCGAGGGTGGGTTTGACTGGTTTCACAAACCTGCACCGAACCCGAACGTGCTGGAGGGAGCAATTGTGACCCCAGATGAAAATGATGGTTTTGCGGACGACAGGAACAACTACCAGTTAGATGAACCAACAACCGTTACTAATGCTCCACTGGTTGGTGTCTTAGCTTACCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

492

Amino Acids

53.67

Weight (kDa)

5.3

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 33 - 389 6.6e-117 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 854
Acc36I ACCTGC 1 cut(s) 1336
AccB7I CCANNNNNTGG 2 cut(s) 181, 1451
AccI GTMKAC 2 cut(s) 708, 929
AciI CCGC 4 cut(s) 549, 561, 999, 1391
AclWI GGATC 5 cut(s) 55, 899, 1228, 1274, 1287
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 3 cut(s) 851, 946, 1239
AcuI CTGAAG 2 cut(s) 444, 496
AfaI GTAC 1 cut(s) 785
AfiI CCNNNNNNNGG 7 cut(s) 181, 445, 501, 532, 698, 720, 1451
AgsI TTSAA 1 cut(s) 1185
AhlI ACTAGT 1 cut(s) 1064
AjnI CCWGG 1 cut(s) 1114
AluBI AGCT 8 cut(s) 274, 340, 358, 643, 936, 1203, 1286, 1463
AluI AGCT 8 cut(s) 274, 340, 358, 643, 936, 1203, 1286, 1463
Alw21I GWGCWC 2 cut(s) 320, 865
Alw26I GTCTC 3 cut(s) 255, 472, 1270
Alw44I GTGCAC 1 cut(s) 861
AlwI GGATC 5 cut(s) 55, 899, 1228, 1274, 1287
Ama87I CYCGRG 2 cut(s) 532, 788
AoxI GGCC 3 cut(s) 288, 436, 1098
ApaLI GTGCAC 1 cut(s) 861
ApeKI GCWGC 8 cut(s) 563, 572, 575, 640, 746, 749, 875, 1253
ApoI RAATTY 3 cut(s) 851, 946, 1239
Asp700I GAANNNNTTC 1 cut(s) 509
AspS9I GGNCC 1 cut(s) 460
AsuC2I CCSGG 3 cut(s) 533, 534, 669
AsuHPI GGTGA 2 cut(s) 1121, 1303
AvaI CYCGRG 2 cut(s) 532, 788
AvaII GGWCC 1 cut(s) 460
AxyI CCTNAGG 1 cut(s) 693
BaeGI GKGCMC 1 cut(s) 865
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 1279
BanII GRGCYC 1 cut(s) 539
BbsI GAAGAC 3 cut(s) 22, 502, 998
Bbv12I GWGCWC 2 cut(s) 320, 865
BbvI GCAGC 8 cut(s) 550, 559, 562, 652, 758, 761, 862, 1265
BccI CCATC 6 cut(s) 206, 280, 471, 768, 1112, 1376
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 1 cut(s) 1116
BcnI CCSGG 3 cut(s) 533, 534, 669
BcoDI GTCTC 3 cut(s) 255, 472, 1270
BcuI ACTAGT 1 cut(s) 1064
BfaI CTAG 1 cut(s) 1065
BfmI CTRYAG 1 cut(s) 588
BfuAI ACCTGC 1 cut(s) 1336
Bme1390I CCNGG 4 cut(s) 533, 534, 669, 1116
Bme18I GGWCC 1 cut(s) 460
BmeT110I CYCGRG 2 cut(s) 532, 788
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 2 cut(s) 758, 1281
BmrFI CCNGG 4 cut(s) 533, 534, 669, 1116
BmsI GCATC 5 cut(s) 211, 244, 696, 726, 1265
BpiI GAAGAC 3 cut(s) 22, 502, 998
BplI GAGNNNNNCTC 2 cut(s) 252, 284
BpmI CTGGAG 1 cut(s) 1370
Bpu10I CCTNAGC 3 cut(s) 145, 341, 1233
BpuEI CTTGAG 2 cut(s) 254, 484
BpuMI CCSGG 3 cut(s) 533, 534, 669
BsaBI GATNNNNATC 1 cut(s) 1219
BsaI GGTCTC 1 cut(s) 472
BsaJI CCNNGG 4 cut(s) 73, 331, 532, 1022
BsaWI WCCGGW 1 cut(s) 1288
BsaXI ACNNNNNCTCC 4 cut(s) 1347, 1377, 1426, 1456
Bsc4I CCNNNNNNNGG 7 cut(s) 181, 445, 501, 532, 698, 720, 1451
Bse1I ACTGG 4 cut(s) 714, 1319, 1411, 1452
Bse21I CCTNAGG 1 cut(s) 693
Bse3DI GCAATG 2 cut(s) 63, 885
Bse8I GATNNNNATC 1 cut(s) 1219
BseBI CCWGG 1 cut(s) 1116
BseDI CCNNGG 4 cut(s) 73, 331, 532, 1022
BseGI GGATG 5 cut(s) 412, 614, 664, 687, 850
BseJI GATNNNNATC 1 cut(s) 1219
BseLI CCNNNNNNNGG 7 cut(s) 181, 445, 501, 532, 698, 720, 1451
BseMI GCAATG 2 cut(s) 63, 885
BseMII CTCAG 3 cut(s) 159, 355, 684
BseNI ACTGG 4 cut(s) 714, 1319, 1411, 1452
BseRI GAGGAG 1 cut(s) 207
BseSI GKGCMC 1 cut(s) 865
BseXI GCAGC 8 cut(s) 550, 559, 562, 652, 758, 761, 862, 1265
BseYI CCCAGC 1 cut(s) 274
BsgI GTGCAG 3 cut(s) 32, 1007, 1314
BshFI GGCC 3 cut(s) 290, 438, 1100
BsiHKAI GWGCWC 2 cut(s) 320, 865
BsiHKCI CYCGRG 2 cut(s) 532, 788
BsiSI CCGG 5 cut(s) 258, 533, 669, 807, 1289
BslI CCNNNNNNNGG 7 cut(s) 181, 445, 501, 532, 698, 720, 1451
BsmAI GTCTC 3 cut(s) 255, 472, 1270
BsmBI CGTCTC 1 cut(s) 1270
BsmI GAATGC 2 cut(s) 963, 1148
BsnI GGCC 3 cut(s) 290, 438, 1100
Bso31I GGTCTC 1 cut(s) 472
BsoBI CYCGRG 2 cut(s) 532, 788
Bsp1286I GDGCHC 3 cut(s) 320, 539, 865
Bsp143I GATC 6 cut(s) 47, 541, 904, 1138, 1220, 1279
Bsp19I CCATGG 1 cut(s) 1022
BspACI CCGC 4 cut(s) 549, 561, 999, 1391
BspANI GGCC 3 cut(s) 290, 438, 1100
BspCNI CTCAG 3 cut(s) 158, 354, 685
BspLI GGNNCC 2 cut(s) 758, 1281
BspMI ACCTGC 1 cut(s) 1336
BspPI GGATC 5 cut(s) 55, 899, 1228, 1274, 1287
BspQI GCTCTTC 1 cut(s) 375
BspTNI GGTCTC 1 cut(s) 472
BsrDI GCAATG 2 cut(s) 63, 885
BsrI ACTGG 4 cut(s) 714, 1319, 1411, 1452
BssECI CCNNGG 4 cut(s) 73, 331, 532, 1022
BssMI GATC 6 cut(s) 47, 541, 904, 1138, 1220, 1279
BssNAI GTATAC 1 cut(s) 930
BssT1I CCWWGG 2 cut(s) 73, 1022
Bst1107I GTATAC 1 cut(s) 930
Bst2UI CCWGG 1 cut(s) 1116
Bst4CI ACNGT 3 cut(s) 298, 1112, 1432
Bst6I CTCTTC 1 cut(s) 375
BstC8I GCNNGC 4 cut(s) 338, 658, 1003, 1007
BstDEI CTNAG 6 cut(s) 145, 341, 693, 797, 1233, 1459
BstDSI CCRYGG 2 cut(s) 331, 1022
BstENI CCTNNNNNAGG 1 cut(s) 696
BstF5I GGATG 5 cut(s) 412, 614, 664, 687, 850
BstKTI GATC 6 cut(s) 50, 544, 907, 1141, 1223, 1282
BstMAI GTCTC 3 cut(s) 255, 472, 1270
BstMBI GATC 6 cut(s) 47, 541, 904, 1138, 1220, 1279
BstMWI GCNNNNNNNGC 9 cut(s) 89, 374, 427, 557, 569, 572, 584, 1097, 1469
BstNI CCWGG 1 cut(s) 1116
BstNSI RCATGY 1 cut(s) 1299
BstSCI CCNGG 4 cut(s) 531, 532, 667, 1114
BstSFI CTRYAG 1 cut(s) 588
BstSLI GKGCMC 1 cut(s) 865
BstV1I GCAGC 8 cut(s) 550, 559, 562, 652, 758, 761, 862, 1265
BstV2I GAAGAC 3 cut(s) 22, 502, 998
BstX2I RGATCY 1 cut(s) 1279
BstYI RGATCY 1 cut(s) 1279
BstZ17I GTATAC 1 cut(s) 930
Bsu36I CCTNAGG 1 cut(s) 693
BsuRI GGCC 3 cut(s) 290, 438, 1100
BtgI CCRYGG 2 cut(s) 331, 1022
BtsCI GGATG 5 cut(s) 412, 614, 664, 687, 850
BtsI GCAGTG 2 cut(s) 223, 1047
BtsIMutI CAGTG 3 cut(s) 223, 1047, 1445
BveI ACCTGC 1 cut(s) 1336
Cac8I GCNNGC 4 cut(s) 338, 658, 1003, 1007
Cfr13I GGNCC 1 cut(s) 460
Cfr9I CCCGGG 1 cut(s) 532
Csp6I GTAC 1 cut(s) 784
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 6 cut(s) 145, 341, 693, 797, 1233, 1459
DpnI GATC 6 cut(s) 49, 543, 906, 1140, 1222, 1281
DpnII GATC 6 cut(s) 47, 541, 904, 1138, 1220, 1279
DrdI GACNNNNNNGTC 1 cut(s) 854
DseDI GACNNNNNNGTC 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 2 cut(s) 73, 1022
Eco147I AGGCCT 1 cut(s) 1100
Eco24I GRGCYC 1 cut(s) 539
Eco31I GGTCTC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 2 cut(s) 444, 496
Eco81I CCTNAGG 1 cut(s) 693
Eco88I CYCGRG 2 cut(s) 532, 788
EcoNI CCTNNNNNAGG 1 cut(s) 696
EcoRII CCWGG 1 cut(s) 1114
EcoT14I CCWWGG 2 cut(s) 73, 1022
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 2 cut(s) 73, 1022
Esp3I CGTCTC 1 cut(s) 1270
FalI AAGNNNNNCTT 4 cut(s) 169, 201, 629, 661
FauI CCCGC 1 cut(s) 1006
FauNDI CATATG 1 cut(s) 953
FblI GTMKAC 2 cut(s) 708, 929
FokI GGATG 5 cut(s) 399, 601, 651, 674, 857
FriOI GRGCYC 1 cut(s) 539
FspBI CTAG 1 cut(s) 1065
GsaI CCCAGC 1 cut(s) 278
GsuI CTGGAG 1 cut(s) 1370
HaeIII GGCC 3 cut(s) 290, 438, 1100
HapII CCGG 5 cut(s) 258, 533, 669, 807, 1289
HincII GTYRAC 2 cut(s) 140, 156
HindII GTYRAC 2 cut(s) 140, 156
HinfI GANTC 4 cut(s) 157, 197, 204, 455
HpaII CCGG 5 cut(s) 258, 533, 669, 807, 1289
HphI GGTGA 2 cut(s) 1121, 1303
Hpy166II GTNNAC 5 cut(s) 140, 156, 709, 863, 930
Hpy188I TCNGA 5 cut(s) 285, 460, 541, 969, 1138
Hpy188III TCNNGA 6 cut(s) 147, 201, 514, 680, 728, 1273
Hpy8I GTNNAC 5 cut(s) 140, 156, 709, 863, 930
HpyAV CCTTC 3 cut(s) 301, 770, 886
HpyCH4III ACNGT 3 cut(s) 298, 1112, 1432
HpyCH4IV ACGT 2 cut(s) 984, 1344
HpyCH4V TGCA 9 cut(s) 13, 92, 635, 863, 878, 941, 963, 988, 1331
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 374, 427, 557, 569, 572, 584, 1097, 1469
HpyF3I CTNAG 6 cut(s) 145, 341, 693, 797, 1233, 1459
HpySE526I ACGT 2 cut(s) 984, 1344
Kzo9I GATC 6 cut(s) 47, 541, 904, 1138, 1220, 1279
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 5 cut(s) 315, 762, 1291, 1355, 1447
Lsp1109I GCAGC 8 cut(s) 550, 559, 562, 652, 758, 761, 862, 1265
LweI GCATC 5 cut(s) 211, 244, 696, 726, 1265
MaeI CTAG 1 cut(s) 1065
MaeII ACGT 2 cut(s) 984, 1344
MaeIII GTNAC 7 cut(s) 298, 608, 625, 1060, 1291, 1363, 1432
MalI GATC 6 cut(s) 49, 543, 906, 1140, 1222, 1281
MboI GATC 6 cut(s) 47, 541, 904, 1138, 1220, 1279
MboII GAAGA 4 cut(s) 22, 362, 502, 1003
MfeI CAATTG 1 cut(s) 1359
MflI RGATCY 1 cut(s) 1279
MhlI GDGCHC 3 cut(s) 320, 539, 865
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 7 cut(s) 78, 228, 851, 946, 1225, 1239, 1359
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 2 cut(s) 151, 198
MmeI TCCRAC 1 cut(s) 1192
Mox20I TGGCCA 1 cut(s) 438
MroXI GAANNNNTTC 1 cut(s) 509
MscI TGGCCA 1 cut(s) 438
MseI TTAA 1 cut(s) 1474
MslI CAYNNNNRTG 2 cut(s) 669, 838
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 2 cut(s) 551, 563
MspI CCGG 5 cut(s) 258, 533, 669, 807, 1289
MspR9I CCNGG 4 cut(s) 533, 534, 669, 1116
MunI CAATTG 1 cut(s) 1359
Mva1269I GAATGC 2 cut(s) 963, 1148
MvaI CCWGG 1 cut(s) 1116
MwoI GCNNNNNNNGC 9 cut(s) 89, 374, 427, 557, 569, 572, 584, 1097, 1469
NciI CCSGG 3 cut(s) 533, 534, 669
NcoI CCATGG 1 cut(s) 1022
NdeI CATATG 1 cut(s) 953
NdeII GATC 6 cut(s) 47, 541, 904, 1138, 1220, 1279
NlaIV GGNNCC 2 cut(s) 758, 1281
NmuCI GTSAC 2 cut(s) 1291, 1363
NspI RCATGY 1 cut(s) 1299
PaeR7I CTCGAG 1 cut(s) 788
PceI AGGCCT 1 cut(s) 1100
PciSI GCTCTTC 1 cut(s) 375
PctI GAATGC 2 cut(s) 963, 1148
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 2 cut(s) 197, 455
PflMI CCANNNNNTGG 2 cut(s) 181, 1451
PleI GAGTC 2 cut(s) 151, 198
PpsI GAGTC 2 cut(s) 151, 198
Psp6I CCWGG 1 cut(s) 1114
PspFI CCCAGC 1 cut(s) 274
PspGI CCWGG 1 cut(s) 1114
PspN4I GGNNCC 2 cut(s) 758, 1281
PspPI GGNCC 1 cut(s) 460
PspXI VCTCGAGB 1 cut(s) 788
PsrI GAACNNNNNNTAC 2 cut(s) 776, 808
PsuI RGATCY 1 cut(s) 1279
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
RseI CAYNNNNRTG 2 cut(s) 669, 838
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 1474
Sau3AI GATC 6 cut(s) 47, 541, 904, 1138, 1220, 1279
Sau96I GGNCC 1 cut(s) 460
SchI GAGTC 2 cut(s) 151, 198
ScrFI CCNGG 4 cut(s) 533, 534, 669, 1116
SduI GDGCHC 3 cut(s) 320, 539, 865
SfaNI GCATC 5 cut(s) 211, 244, 696, 726, 1265
SfcI CTRYAG 1 cut(s) 588
Sfr274I CTCGAG 1 cut(s) 788
SinI GGWCC 1 cut(s) 460
SlaI CTCGAG 1 cut(s) 788
SmaI CCCGGG 1 cut(s) 534
SmiMI CAYNNNNRTG 2 cut(s) 669, 838
SmlI CTYRAG 3 cut(s) 269, 499, 788
SmoI CTYRAG 3 cut(s) 269, 499, 788
SpeI ACTAGT 1 cut(s) 1064
Sse9I AATT 7 cut(s) 78, 228, 851, 946, 1225, 1239, 1359
SseBI AGGCCT 1 cut(s) 1100
SsiI CCGC 4 cut(s) 549, 561, 999, 1391
SspMI CTAG 1 cut(s) 1065
StuI AGGCCT 1 cut(s) 1100
StyD4I CCNGG 4 cut(s) 531, 532, 667, 1114
StyI CCWWGG 2 cut(s) 73, 1022
TaaI ACNGT 3 cut(s) 298, 1112, 1432
TaiI ACGT 2 cut(s) 987, 1347
TaqI TCGA 5 cut(s) 324, 581, 600, 789, 1223
TasI AATT 7 cut(s) 78, 228, 851, 946, 1225, 1239, 1359
TauI GCSGC 2 cut(s) 551, 563
TfiI GAWTC 2 cut(s) 197, 455
Tru1I TTAA 1 cut(s) 1474
Tru9I TTAA 1 cut(s) 1474
TscAI CASTG 3 cut(s) 223, 1054, 1452
TseFI GTSAC 2 cut(s) 1291, 1363
TseI GCWGC 8 cut(s) 563, 572, 575, 640, 746, 749, 875, 1253
Tsp45I GTSAC 2 cut(s) 1291, 1363
TspDTI ATGAA 8 cut(s) 300, 789, 905, 940, 1044, 1232, 1389, 1434
TspGWI ACGGA 2 cut(s) 1066, 1292
TspMI CCCGGG 1 cut(s) 532
TspRI CASTG 3 cut(s) 223, 1054, 1452
Van91I CCANNNNNTGG 2 cut(s) 181, 1451
VneI GTGCAC 1 cut(s) 861
VpaK11BI GGWCC 1 cut(s) 460
XagI CCTNNNNNAGG 1 cut(s) 696
XapI RAATTY 3 cut(s) 851, 946, 1239
XceI RCATGY 1 cut(s) 1299
XcmI CCANNNNNNNNNTGG 1 cut(s) 1162
XhoI CTCGAG 1 cut(s) 788
XmaI CCCGGG 1 cut(s) 532
XmiI GTMKAC 2 cut(s) 708, 929
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 1 cut(s) 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.