Rh4AG434800

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003358
Physical Location & Seq
Reverse (-)
72884364 .. 72886109
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG434800.1

Sequence Viewer

Length: 1263 bp
ATGCGGATTGATTTGGTGGGAGGCTACTACGATGCCGGAGACAATCTCAAGTTGGGATTTCCAATGGCCTTCTCAGTAACAATGCTGTCATGGAGCACGATCGAATTCCAAAGCAAGCTCAAGGCCAAATATGAGATCGCTAATGCTCTTCGGGCTATCAAGTGGGGAACAGATTACTTGATAAAAGCACATCCACAGCCCGAAGTGCTTTATGGGCATATTGGGGGTGTTGATTCAGACCATCAATGTTGGCAGAGACCAGAGGACATGACCACCCCGAGGACCGTGTTCATGATTGATGACCAACACCCGGGATCTGATCTCGCCGCTGAAACTGCTGCTGCTTTGGCTGCTGCCTCAATTGCTTTTGAACGTTTTGATAGAAGTTACTCATACCAACTCGTAATTCATGCAAAGCAGCTATTTGATTTTGCACGCAATCATCCTGGTAAGTATCAAAACAGCATCCCTGAGGCAGGAAAAGTCTACGCCAGTAGTGGGTATCAGGACGAGATGCTGTGGGCAGCGGCATGGCTCCATCGTGCTACTAATGAACAGAAGTACCTCAACTTCCTAAGCGAAGCGGGAGATATAGGTGGAACAAGAACAACATTCTCATGGGATGACAAATTCGTCGGCGCACAGGTTTTGGCAGCACTGCTTGTAATGAAGGGAAAGGTTGATTCTTCAGGAAAATGGGGGGAGTATAAGCGCCATGCAGAGGAATTCATATGTTCATGCGTTCAGAAAGGAAACAACAACGTGAAGAAGACACCCGGAGGCTTGCTTTGGTTTCTGCCATGGAATAACAATCAATACGTTGCCACTGCTACATTTGTCACCACTGTTTATTCCGTTTATTTGGACGCAAAAAAGGCCTCCATCAACTGTCCTGGTGGCGCTGCTCACCCCTCTGATCTCATTGCATCTGCCAAGTCACAGGTTGATTACATCTTGGGCTCAAACCCGAAAAACATGAGCTATATGGTTGGATATGGATCGAATTATCCGAAGCAAATTCATCACAGAGGAGCATCAATCGTCTCAATCAAGAAGGATCCAGCTCCTGTAACATGCCAAGGTGGGTTTGACTGGTTTCACAGACCCGAGCCCAACCCGAACGTGCTGGAGGGAGCAATTGTGACCCCAGATGAAAATGATGGTTTTGCGGACGACAGGAACAACTTTCAGTTAGCTGAACCAACGACCGTTACTAATGCTCCACTAGTTGGTGTCTTAGCTCACCTTGCTCAACTCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

420

Amino Acids

46.39

Weight (kDa)

6.23

Isoelectric Point (pI)

26.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 3 - 413 4.5e-138 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 632
AccB7I CCANNNNNTGG 1 cut(s) 1229
AccI GTMKAC 1 cut(s) 486
AciI CCGC 5 cut(s) 4, 327, 527, 584, 1169
AclI AACGTT 1 cut(s) 373
AclWI GGATC 4 cut(s) 322, 1006, 1052, 1065
AcsI RAATTY 4 cut(s) 104, 629, 725, 1017
AcuI CTGAAG 1 cut(s) 672
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 6 cut(s) 279, 310, 476, 498, 721, 1229
AgsI TTSAA 1 cut(s) 371
AhlI ACTAGT 1 cut(s) 1225
AjnI CCWGG 2 cut(s) 445, 892
AjuI GAANNNNNNNTTGG 2 cut(s) 390, 422
AluBI AGCT 6 cut(s) 118, 421, 981, 1064, 1196, 1241
AluI AGCT 6 cut(s) 118, 421, 981, 1064, 1196, 1241
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 3 cut(s) 33, 250, 1048
AlwI GGATC 4 cut(s) 322, 1006, 1052, 1065
AlwNI CAGNNNCTG 1 cut(s) 1067
Ama87I CYCGRG 3 cut(s) 277, 310, 1106
AoxI GGCC 3 cut(s) 66, 123, 876
ApeKI GCWGC 8 cut(s) 338, 341, 350, 353, 418, 524, 653, 902
ApoI RAATTY 4 cut(s) 104, 629, 725, 1017
AspLEI GCGC 3 cut(s) 641, 714, 902
AspS9I GGNCC 1 cut(s) 282
AsuC2I CCSGG 3 cut(s) 311, 312, 777
AsuHPI GGTGA 3 cut(s) 832, 899, 1235
AvaI CYCGRG 3 cut(s) 277, 310, 1106
AvaII GGWCC 1 cut(s) 282
AxyI CCTNAGG 1 cut(s) 471
BaeI ACNNNNGTAYC 2 cut(s) 545, 578
BamHI GGATCC 1 cut(s) 1057
BanII GRGCYC 2 cut(s) 962, 1113
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 8 cut(s) 325, 328, 337, 340, 430, 536, 665, 889
BccI CCATC 4 cut(s) 249, 546, 890, 1154
BciT130I CCWGG 2 cut(s) 447, 894
BcnI CCSGG 3 cut(s) 311, 312, 777
BcoDI GTCTC 3 cut(s) 33, 250, 1048
BcuI ACTAGT 1 cut(s) 1225
BfaI CTAG 1 cut(s) 1226
BfoI RGCGCY 2 cut(s) 715, 903
Bme1390I CCNGG 5 cut(s) 311, 312, 447, 777, 894
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 3 cut(s) 277, 310, 1106
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 536, 1059
BmrFI CCNGG 5 cut(s) 311, 312, 447, 777, 894
BmsI GCATC 5 cut(s) 22, 474, 504, 935, 1043
BpiI GAAGAC 1 cut(s) 776
BplI GAGNNNNNCTC 2 cut(s) 30, 62
BpmI CTGGAG 1 cut(s) 1148
Bpu10I CCTNAGC 1 cut(s) 575
BpuEI CTTGAG 2 cut(s) 32, 104
BpuMI CCSGG 3 cut(s) 311, 312, 777
BsaBI GATNNNNATC 1 cut(s) 997
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 4 cut(s) 278, 310, 800, 1078
BsaXI ACNNNNNCTCC 4 cut(s) 1125, 1155, 1204, 1234
Bsc4I CCNNNNNNNGG 6 cut(s) 279, 310, 476, 498, 721, 1229
Bse1I ACTGG 2 cut(s) 492, 1097
Bse21I CCTNAGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 921
Bse8I GATNNNNATC 1 cut(s) 997
BseBI CCWGG 2 cut(s) 447, 894
BseDI CCNNGG 4 cut(s) 278, 310, 800, 1078
BseGI GGATG 4 cut(s) 190, 442, 465, 628
BseJI GATNNNNATC 1 cut(s) 997
BseLI CCNNNNNNNGG 6 cut(s) 279, 310, 476, 498, 721, 1229
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 2 cut(s) 87, 462
BseNI ACTGG 2 cut(s) 492, 1097
BseRI GAGGAG 1 cut(s) 1044
BseXI GCAGC 8 cut(s) 325, 328, 337, 340, 430, 536, 665, 889
Bsh1285I CGRYCG 2 cut(s) 102, 1209
BshFI GGCC 3 cut(s) 68, 125, 878
BsiEI CGRYCG 2 cut(s) 102, 1209
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 3 cut(s) 277, 310, 1106
BsiSI CCGG 3 cut(s) 36, 311, 777
BslI CCNNNNNNNGG 6 cut(s) 279, 310, 476, 498, 721, 1229
BsmAI GTCTC 3 cut(s) 33, 250, 1048
BsmBI CGTCTC 1 cut(s) 1048
BsnI GGCC 3 cut(s) 68, 125, 878
Bso31I GGTCTC 1 cut(s) 250
BsoBI CYCGRG 3 cut(s) 277, 310, 1106
Bsp1286I GDGCHC 3 cut(s) 98, 962, 1113
Bsp143I GATC 7 cut(s) 99, 135, 314, 319, 916, 998, 1057
Bsp19I CCATGG 1 cut(s) 800
BspACI CCGC 5 cut(s) 4, 327, 527, 584, 1169
BspANI GGCC 3 cut(s) 68, 125, 878
BspCNI CTCAG 2 cut(s) 86, 463
BspHI TCATGA 1 cut(s) 291
BspLI GGNNCC 2 cut(s) 536, 1059
BspPI GGATC 4 cut(s) 322, 1006, 1052, 1065
BspQI GCTCTTC 1 cut(s) 153
BspTNI GGTCTC 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 921
BsrI ACTGG 2 cut(s) 492, 1097
BssECI CCNNGG 4 cut(s) 278, 310, 800, 1078
BssMI GATC 7 cut(s) 99, 135, 314, 319, 916, 998, 1057
BssT1I CCWWGG 2 cut(s) 800, 1078
Bst2UI CCWGG 2 cut(s) 447, 894
Bst4CI ACNGT 4 cut(s) 286, 847, 890, 1210
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 3 cut(s) 116, 436, 785
BstDEI CTNAG 4 cut(s) 73, 471, 575, 1237
BstDSI CCRYGG 1 cut(s) 800
BstENI CCTNNNNNAGG 1 cut(s) 474
BstF5I GGATG 4 cut(s) 190, 442, 465, 628
BstH2I RGCGCY 2 cut(s) 715, 903
BstHHI GCGC 3 cut(s) 641, 714, 902
BstKTI GATC 7 cut(s) 102, 138, 317, 322, 919, 1001, 1060
BstMAI GTCTC 3 cut(s) 33, 250, 1048
BstMBI GATC 7 cut(s) 99, 135, 314, 319, 916, 998, 1057
BstMCI CGRYCG 2 cut(s) 102, 1209
BstMWI GCNNNNNNNGC 9 cut(s) 152, 205, 214, 335, 347, 350, 362, 875, 1247
BstNI CCWGG 2 cut(s) 447, 894
BstNSI RCATGY 1 cut(s) 1077
BstSCI CCNGG 5 cut(s) 309, 310, 445, 775, 892
BstV1I GCAGC 8 cut(s) 325, 328, 337, 340, 430, 536, 665, 889
BstV2I GAAGAC 1 cut(s) 776
BstX2I RGATCY 2 cut(s) 314, 1057
BstYI RGATCY 2 cut(s) 314, 1057
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 3 cut(s) 68, 125, 878
BtgI CCRYGG 1 cut(s) 800
BtsCI GGATG 4 cut(s) 190, 442, 465, 628
BtsI GCAGTG 2 cut(s) 656, 825
BtsIMutI CAGTG 3 cut(s) 656, 825, 843
Cac8I GCNNGC 3 cut(s) 116, 436, 785
CaiI CAGNNNCTG 1 cut(s) 1067
CciI TCATGA 1 cut(s) 291
CfoI GCGC 3 cut(s) 641, 714, 902
Cfr13I GGNCC 1 cut(s) 282
Cfr9I CCCGGG 1 cut(s) 310
CseI GACGC 1 cut(s) 875
Csp6I GTAC 1 cut(s) 562
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 4 cut(s) 73, 471, 575, 1237
DpnI GATC 7 cut(s) 101, 137, 316, 321, 918, 1000, 1059
DpnII GATC 7 cut(s) 99, 135, 314, 319, 916, 998, 1057
DrdI GACNNNNNNGTC 1 cut(s) 632
DseDI GACNNNNNNGTC 1 cut(s) 632
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco130I CCWWGG 2 cut(s) 800, 1078
Eco147I AGGCCT 1 cut(s) 878
Eco24I GRGCYC 2 cut(s) 962, 1113
Eco31I GGTCTC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 672
Eco81I CCTNAGG 1 cut(s) 471
Eco88I CYCGRG 3 cut(s) 277, 310, 1106
EcoNI CCTNNNNNAGG 1 cut(s) 474
EcoRI GAATTC 2 cut(s) 104, 725
EcoRII CCWGG 2 cut(s) 445, 892
EcoT14I CCWWGG 2 cut(s) 800, 1078
EcoT38I GRGCYC 2 cut(s) 962, 1113
ErhI CCWWGG 2 cut(s) 800, 1078
Esp3I CGTCTC 1 cut(s) 1048
FauI CCCGC 1 cut(s) 577
FauNDI CATATG 1 cut(s) 731
FblI GTMKAC 1 cut(s) 486
FokI GGATG 4 cut(s) 177, 429, 452, 635
FriOI GRGCYC 2 cut(s) 962, 1113
FspBI CTAG 1 cut(s) 1226
GlaI GCGC 3 cut(s) 640, 713, 901
GsuI CTGGAG 1 cut(s) 1148
HaeII RGCGCY 2 cut(s) 715, 903
HaeIII GGCC 3 cut(s) 68, 125, 878
HapII CCGG 3 cut(s) 36, 311, 777
HgaI GACGC 1 cut(s) 875
HhaI GCGC 3 cut(s) 641, 714, 902
Hin6I GCGC 3 cut(s) 639, 712, 900
HinP1I GCGC 3 cut(s) 639, 712, 900
HinfI GANTC 2 cut(s) 233, 683
HpaII CCGG 3 cut(s) 36, 311, 777
HphI GGTGA 3 cut(s) 832, 899, 1235
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 5 cut(s) 238, 319, 747, 916, 1011
Hpy188III TCNNGA 4 cut(s) 292, 506, 690, 1051
Hpy8I GTNNAC 1 cut(s) 487
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 3 cut(s) 79, 664, 1048
HpyCH4III ACNGT 4 cut(s) 286, 847, 890, 1210
HpyCH4IV ACGT 4 cut(s) 373, 762, 819, 1122
HpyCH4V TGCA 4 cut(s) 413, 434, 719, 926
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 205, 214, 335, 347, 350, 362, 875, 1247
HpyF3I CTNAG 4 cut(s) 73, 471, 575, 1237
HpySE526I ACGT 4 cut(s) 373, 762, 819, 1122
HspAI GCGC 3 cut(s) 639, 712, 900
Kzo9I GATC 7 cut(s) 99, 135, 314, 319, 916, 998, 1057
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 6 cut(s) 93, 540, 1031, 1069, 1133, 1225
Lsp1109I GCAGC 8 cut(s) 325, 328, 337, 340, 430, 536, 665, 889
LweI GCATC 5 cut(s) 22, 474, 504, 935, 1043
MaeI CTAG 1 cut(s) 1226
MaeII ACGT 4 cut(s) 373, 762, 819, 1122
MaeIII GTNAC 7 cut(s) 76, 386, 838, 936, 1069, 1141, 1210
MalI GATC 7 cut(s) 101, 137, 316, 321, 918, 1000, 1059
MboI GATC 7 cut(s) 99, 135, 314, 319, 916, 998, 1057
MboII GAAGA 4 cut(s) 140, 678, 778, 781
MfeI CAATTG 2 cut(s) 360, 1137
MflI RGATCY 2 cut(s) 314, 1057
MhlI GDGCHC 3 cut(s) 98, 962, 1113
MluCI AATT 8 cut(s) 104, 360, 405, 629, 725, 1003, 1017, 1137
MmeI TCCRAC 1 cut(s) 970
MslI CAYNNNNRTG 1 cut(s) 616
MspA1I CMGCKG 2 cut(s) 329, 527
MspI CCGG 3 cut(s) 36, 311, 777
MspR9I CCNGG 5 cut(s) 311, 312, 447, 777, 894
MunI CAATTG 2 cut(s) 360, 1137
MvaI CCWGG 2 cut(s) 447, 894
MwoI GCNNNNNNNGC 9 cut(s) 152, 205, 214, 335, 347, 350, 362, 875, 1247
NciI CCSGG 3 cut(s) 311, 312, 777
NcoI CCATGG 1 cut(s) 800
NdeI CATATG 1 cut(s) 731
NdeII GATC 7 cut(s) 99, 135, 314, 319, 916, 998, 1057
NlaIV GGNNCC 2 cut(s) 536, 1059
NmuCI GTSAC 3 cut(s) 838, 936, 1141
NspI RCATGY 1 cut(s) 1077
PagI TCATGA 1 cut(s) 291
PceI AGGCCT 1 cut(s) 878
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 1007
PfeI GAWTC 2 cut(s) 233, 683
PflMI CCANNNNNTGG 1 cut(s) 1229
Ple19I CGATCG 1 cut(s) 102
Psp1406I AACGTT 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 445, 892
PspGI CCWGG 2 cut(s) 445, 892
PspN4I GGNNCC 2 cut(s) 536, 1059
PspPI GGNCC 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 1067
PsuI RGATCY 2 cut(s) 314, 1057
PvuI CGATCG 1 cut(s) 102
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 1 cut(s) 616
SapI GCTCTTC 1 cut(s) 153
Sau3AI GATC 7 cut(s) 99, 135, 314, 319, 916, 998, 1057
Sau96I GGNCC 1 cut(s) 282
ScrFI CCNGG 5 cut(s) 311, 312, 447, 777, 894
SduI GDGCHC 3 cut(s) 98, 962, 1113
SfaNI GCATC 5 cut(s) 22, 474, 504, 935, 1043
SinI GGWCC 1 cut(s) 282
SmaI CCCGGG 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 616
SmlI CTYRAG 2 cut(s) 47, 119
SmoI CTYRAG 2 cut(s) 47, 119
SpeI ACTAGT 1 cut(s) 1225
Sse9I AATT 8 cut(s) 104, 360, 405, 629, 725, 1003, 1017, 1137
SseBI AGGCCT 1 cut(s) 878
SsiI CCGC 5 cut(s) 4, 327, 527, 584, 1169
SspMI CTAG 1 cut(s) 1226
StuI AGGCCT 1 cut(s) 878
StyD4I CCNGG 5 cut(s) 309, 310, 445, 775, 892
StyI CCWWGG 2 cut(s) 800, 1078
TaaI ACNGT 4 cut(s) 286, 847, 890, 1210
TaiI ACGT 4 cut(s) 376, 765, 822, 1125
TaqI TCGA 2 cut(s) 102, 1001
TasI AATT 8 cut(s) 104, 360, 405, 629, 725, 1003, 1017, 1137
TauI GCSGC 2 cut(s) 329, 530
TfiI GAWTC 2 cut(s) 233, 683
TscAI CASTG 3 cut(s) 663, 832, 850
TseFI GTSAC 3 cut(s) 838, 936, 1141
TseI GCWGC 8 cut(s) 338, 341, 350, 353, 418, 524, 653, 902
Tsp45I GTSAC 3 cut(s) 838, 936, 1141
TspDTI ATGAA 8 cut(s) 280, 398, 567, 683, 718, 726, 1010, 1167
TspGWI ACGGA 1 cut(s) 844
TspMI CCCGGG 1 cut(s) 310
TspRI CASTG 3 cut(s) 663, 832, 850
Van91I CCANNNNNTGG 1 cut(s) 1229
VpaK11BI GGWCC 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 474
XapI RAATTY 4 cut(s) 104, 629, 725, 1017
XceI RCATGY 1 cut(s) 1077
XmaI CCCGGG 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 486
XspI CTAG 1 cut(s) 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.