MD13G1006100.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003358
Physical Location & Seq
Forward (+)
383394 .. 385223
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1006100.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGAATCGTACTGGTCGTGTTTTTGGTTTGGTTGGGTGCTTATTGCTGGCAATGCTTCATGGAATTGCGTGTCAGGTAGATTACCATGCAGCACTTACCAAGTCTCTGTTGTATTTTGAGGGTCAACGCTCTGGAAAGTTGCCGGATAATAATAAGCTGCAGAGAGTGCAATGGCGTGGAGATTCTGCACTCAAAGACGGCTCTGATGCTGGAATTGATCTGGTGGGAGGATACTACGATGCCGGAGACAATCTCAAGCTAGGGTTTCCAATGGCATTCACAATAACAATGTTGTCGTGGAGCCAAATAGAGATGGGCAGCCAACTCAAGGCAAAGCAAGAGCTCACAAATGTGTTGCATGCCATCAAGTGGGGAACGGATTACTTGATCAAAGCGCATCCCAAGCCTGAGGTGCTCTACGCGCAGATCGGTGAGCCTGATTCCGACCATGGATGTTGGCAAAGGCCGGAAGACATGGCCACCCCAAGAACCGCCTTCAAGATTGATGACCAACATCCTGGCTCCGATCTTGCTGCCGAAACTGCTGCTGCTTTTGCCGCAGCTTCAATCGCTTTCAAGAATTCGGACCATCAATACTCATTCAAGCTTCTAACGCACGCGAAACAGCTTTTTGATTTTGCATGCAATCACCCTGGTCTTTATCAGGACAGCATCCCTTCTGCCGGAAAAGTCTACGCCAGTAGTGGCTATCATGATGAACTGATGTGGGCAGCAGCATGGCTACATCGTGCTACAAATGAAAAAAGGTACCTGGATCACGTTGAGCAAGCAGAAGACGGAGGGGGAACAAGATCCAAATTCTCATGGGATGACAAGTACGTTGGCGCACAGATCTTGATTGCAAGACTTGTATTGGAGGGAAAGGTTGAGAATGGAGGAAAGTGGGCCGAATACAAGAGCCACGCTGAGGAGTTCATATGCTCGTGCATTCAGAAAGGGAACACCAAAGTGCGGCAGACACCTGGTGGCTTGTTATGGTTTTCACCCTGGGATAACATTCAATACGTAGCCACCGCTACCTTCACCACCATTGTCTATTCCAATTATTTGGCCACCAAATATGCCTCCCTCAACTGTGCTGCTGGCAACGCTAATCCTTCTGATCTTATTGCATTCGTCAAATCACAGGTGGATTATATATTGGGGTCAAACCCCAAGAACATGAGTTACATGATTGGATATGGAGGAAACTATCCACAGCGGATTCATCACAGAGGAGCATCAATAATATCGATCAAGAAGGACTCGACACCGGTGACATGCAAAGGAGTGTATGCCGATTGGTTCAACAGAAAAGCAGTGAATCCAAATGTCCTGGATGGAGCAGTCGTGAGTCCAAATGAAAGTGATGGTTTCTCCGATGATAGAAGCAATTTTCAATTTGGTGAACCCACAACATATAACAATGGACCACTAGTTGGTGTCTTGGCTCACTTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

488

Amino Acids

53.7

Weight (kDa)

6.46

Isoelectric Point (pI)

26.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 483 3.5e-156 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 768
AccB1I GGYRCC 1 cut(s) 768
AccB7I CCANNNNNTGG 2 cut(s) 369, 1439
AccI GTMKAC 1 cut(s) 693
AccII CGCG 2 cut(s) 422, 620
AciI CCGC 5 cut(s) 492, 558, 973, 1035, 1222
AclWI GGATC 2 cut(s) 783, 807
AcoI YGGCCR 2 cut(s) 477, 1071
AcsI RAATTY 2 cut(s) 580, 818
AdeI CACNNNGTG 1 cut(s) 986
AfaI GTAC 3 cut(s) 10, 770, 839
AfiI CCNNNNNNNGG 6 cut(s) 328, 369, 683, 928, 972, 1439
AgeI ACCGGT 1 cut(s) 1273
AgsI TTSAA 7 cut(s) 499, 567, 577, 604, 1022, 1309, 1400
AhlI ACTAGT 1 cut(s) 1435
AjnI CCWGG 6 cut(s) 517, 652, 771, 982, 1007, 1335
AleI CACNNNNGTG 2 cut(s) 350, 968
AluBI AGCT 6 cut(s) 157, 259, 343, 563, 607, 628
AluI AGCT 6 cut(s) 157, 259, 343, 563, 607, 628
Alw21I GWGCWC 2 cut(s) 345, 417
Alw26I GTCTC 2 cut(s) 108, 240
AlwI GGATC 2 cut(s) 783, 807
AoxI GGCC 4 cut(s) 464, 477, 906, 1071
ApoI RAATTY 2 cut(s) 580, 818
AsiGI ACCGGT 1 cut(s) 1273
Asp718I GGTACC 1 cut(s) 768
AspLEI GCGC 3 cut(s) 397, 424, 848
AspS9I GGNCC 3 cut(s) 586, 906, 1430
AsuHPI GGTGA 6 cut(s) 443, 641, 996, 1036, 1288, 1418
AvaII GGWCC 2 cut(s) 586, 1430
AxyI CCTNAGG 1 cut(s) 408
BalI TGGCCA 2 cut(s) 479, 1073
BanI GGYRCC 1 cut(s) 768
BanII GRGCYC 1 cut(s) 345
BauI CACGAG 1 cut(s) 943
BbsI GAAGAC 2 cut(s) 477, 801
Bbv12I GWGCWC 2 cut(s) 345, 417
BbvCI CCTCAGC 1 cut(s) 927
BccI CCATC 5 cut(s) 307, 371, 597, 1334, 1364
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 4 cut(s) 515, 527, 549, 561
BciT130I CCWGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
BciVI GTATCC 1 cut(s) 224
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 2 cut(s) 108, 240
BcuI ACTAGT 1 cut(s) 1435
BfaI CTAG 2 cut(s) 260, 1436
BfmI CTRYAG 1 cut(s) 158
BfuI GTATCC 1 cut(s) 224
BglII AGATCT 1 cut(s) 852
Bme1390I CCNGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
Bme18I GGWCC 2 cut(s) 586, 1430
BmgT120I GGNCC 3 cut(s) 586, 906, 1430
BmiI GGNNCC 3 cut(s) 302, 523, 770
BmrFI CCNGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
BmsI GCATC 5 cut(s) 196, 229, 406, 681, 1250
BpiI GAAGAC 2 cut(s) 477, 801
BplI GAGNNNNNCTC 2 cut(s) 237, 269
Bpu10I CCTNAGC 1 cut(s) 927
BpuEI CTTGAG 2 cut(s) 239, 311
Bsa29I ATCGAT 1 cut(s) 1253
BsaAI YACGTR 1 cut(s) 1027
BsaJI CCNNGG 4 cut(s) 448, 652, 1007, 1008
BsaWI WCCGGW 1 cut(s) 1273
BsaXI ACNNNNNCTCC 2 cut(s) 1335, 1365
Bsc4I CCNNNNNNNGG 6 cut(s) 328, 369, 683, 928, 972, 1439
Bse118I RCCGGY 1 cut(s) 1273
Bse1I ACTGG 2 cut(s) 16, 699
Bse21I CCTNAGG 1 cut(s) 408
Bse3DI GCAATG 2 cut(s) 57, 176
BseBI CCWGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
BseCI ATCGAT 1 cut(s) 1253
BseDI CCNNGG 4 cut(s) 448, 652, 1007, 1008
BseGI GGATG 6 cut(s) 397, 458, 514, 672, 835, 1345
BseLI CCNNNNNNNGG 6 cut(s) 328, 369, 683, 928, 972, 1439
BseMI GCAATG 2 cut(s) 57, 176
BseMII CTCAG 2 cut(s) 399, 918
BseNI ACTGG 2 cut(s) 16, 699
BseRI GAGGAG 2 cut(s) 944, 1251
BsgI GTGCAG 1 cut(s) 171
Bsh1236I CGCG 2 cut(s) 422, 620
BshFI GGCC 4 cut(s) 466, 479, 908, 1073
BshNI GGYRCC 1 cut(s) 768
BshTI ACCGGT 1 cut(s) 1273
BshVI ATCGAT 1 cut(s) 1253
BsiHKAI GWGCWC 2 cut(s) 345, 417
BsiSI CCGG 5 cut(s) 143, 243, 467, 684, 1274
BslI CCNNNNNNNGG 6 cut(s) 328, 369, 683, 928, 972, 1439
BsmAI GTCTC 2 cut(s) 108, 240
BsmI GAATGC 3 cut(s) 275, 948, 1133
BsnI GGCC 4 cut(s) 466, 479, 908, 1073
Bsp1286I GDGCHC 2 cut(s) 345, 417
Bsp143I GATC 9 cut(s) 217, 387, 426, 526, 775, 812, 852, 1123, 1254
Bsp19I CCATGG 1 cut(s) 448
BspACI CCGC 5 cut(s) 492, 558, 973, 1035, 1222
BspANI GGCC 4 cut(s) 466, 479, 908, 1073
BspCNI CTCAG 2 cut(s) 400, 919
BspDI ATCGAT 1 cut(s) 1253
BspFNI CGCG 2 cut(s) 422, 620
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 3 cut(s) 302, 523, 770
BspMAI CTGCAG 1 cut(s) 162
BspPI GGATC 2 cut(s) 783, 807
BspT107I GGYRCC 1 cut(s) 768
BsrDI GCAATG 2 cut(s) 57, 176
BsrFI RCCGGY 1 cut(s) 1273
BsrI ACTGG 2 cut(s) 16, 699
BssAI RCCGGY 1 cut(s) 1273
BssECI CCNNGG 4 cut(s) 448, 652, 1007, 1008
BssMI GATC 9 cut(s) 217, 387, 426, 526, 775, 812, 852, 1123, 1254
BssSI CACGAG 1 cut(s) 943
BssT1I CCWWGG 1 cut(s) 448
Bst2BI CACGAG 1 cut(s) 943
Bst2UI CCWGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
Bst4CI ACNGT 1 cut(s) 1097
BstAPI GCANNNNNTGC 1 cut(s) 166
BstBAI YACGTR 1 cut(s) 1027
BstC8I GCNNGC 6 cut(s) 48, 360, 618, 643, 789, 1105
BstDEI CTNAG 2 cut(s) 408, 927
BstDSI CCRYGG 1 cut(s) 448
BstF5I GGATG 6 cut(s) 397, 458, 514, 672, 835, 1345
BstFNI CGCG 2 cut(s) 422, 620
BstHHI GCGC 3 cut(s) 397, 424, 848
BstKTI GATC 9 cut(s) 220, 390, 429, 529, 778, 815, 855, 1126, 1257
BstMAI GTCTC 2 cut(s) 108, 240
BstMBI GATC 9 cut(s) 217, 387, 426, 526, 775, 812, 852, 1123, 1254
BstNI CCWGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
BstNSI RCATGY 3 cut(s) 362, 645, 1284
BstSCI CCNGG 6 cut(s) 517, 652, 771, 982, 1007, 1335
BstSFI CTRYAG 1 cut(s) 158
BstSNI TACGTA 1 cut(s) 1027
BstUI CGCG 2 cut(s) 422, 620
BstV2I GAAGAC 2 cut(s) 477, 801
BstX2I RGATCY 2 cut(s) 812, 852
BstXI CCANNNNNNTGG 2 cut(s) 518, 1069
BstYI RGATCY 2 cut(s) 812, 852
Bsu15I ATCGAT 1 cut(s) 1253
Bsu36I CCTNAGG 1 cut(s) 408
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 4 cut(s) 466, 479, 908, 1073
BsuTUI ATCGAT 1 cut(s) 1253
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 6 cut(s) 397, 458, 514, 672, 835, 1345
BtsI GCAGTG 1 cut(s) 1326
BtsIMutI CAGTG 1 cut(s) 1326
Cac8I GCNNGC 6 cut(s) 48, 360, 618, 643, 789, 1105
CciI TCATGA 1 cut(s) 712
CfoI GCGC 3 cut(s) 397, 424, 848
Cfr10I RCCGGY 1 cut(s) 1273
Cfr13I GGNCC 3 cut(s) 586, 906, 1430
ClaI ATCGAT 1 cut(s) 1253
CsiI ACCWGGT 1 cut(s) 982
Csp6I GTAC 3 cut(s) 9, 769, 838
CspAI ACCGGT 1 cut(s) 1273
CviQI GTAC 3 cut(s) 9, 769, 838
DdeI CTNAG 2 cut(s) 408, 927
DpnI GATC 9 cut(s) 219, 389, 428, 528, 777, 814, 854, 1125, 1256
DpnII GATC 9 cut(s) 217, 387, 426, 526, 775, 812, 852, 1123, 1254
DraIII CACNNNGTG 1 cut(s) 986
EaeI YGGCCR 2 cut(s) 477, 1071
Ecl136II GAGCTC 1 cut(s) 343
Eco105I TACGTA 1 cut(s) 1027
Eco130I CCWWGG 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 586, 1430
Eco53kI GAGCTC 1 cut(s) 343
Eco81I CCTNAGG 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 343
EcoRI GAATTC 1 cut(s) 580
EcoRII CCWGG 6 cut(s) 517, 652, 771, 982, 1007, 1335
EcoT14I CCWWGG 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 448
FauNDI CATATG 1 cut(s) 938
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 1 cut(s) 693
FokI GGATG 6 cut(s) 384, 465, 501, 659, 842, 1352
FriOI GRGCYC 1 cut(s) 345
FspBI CTAG 2 cut(s) 260, 1436
GlaI GCGC 3 cut(s) 396, 423, 847
HaeIII GGCC 4 cut(s) 466, 479, 908, 1073
HapII CCGG 5 cut(s) 143, 243, 467, 684, 1274
HhaI GCGC 3 cut(s) 397, 424, 848
Hin6I GCGC 3 cut(s) 395, 422, 846
HinP1I GCGC 3 cut(s) 395, 422, 846
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 7 cut(s) 4, 182, 440, 1225, 1265, 1324, 1354
HpaII CCGG 5 cut(s) 143, 243, 467, 684, 1274
HphI GGTGA 6 cut(s) 443, 641, 996, 1036, 1288, 1418
Hpy166II GTNNAC 3 cut(s) 125, 694, 1409
Hpy188I TCNGA 7 cut(s) 205, 445, 526, 586, 954, 1123, 1381
Hpy188III TCNNGA 8 cut(s) 132, 499, 577, 665, 713, 856, 1258, 1351
Hpy8I GTNNAC 3 cut(s) 125, 694, 1409
HpyAV CCTTC 5 cut(s) 505, 687, 1051, 1128, 1255
HpyCH4III ACNGT 1 cut(s) 1097
HpyCH4IV ACGT 3 cut(s) 780, 840, 1026
HpyF3I CTNAG 2 cut(s) 408, 927
HpySE526I ACGT 3 cut(s) 780, 840, 1026
HspAI GCGC 3 cut(s) 395, 422, 846
KpnI GGTACC 1 cut(s) 772
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 9 cut(s) 217, 387, 426, 526, 775, 812, 852, 1123, 1254
LmnI GCTCC 4 cut(s) 300, 527, 1238, 1343
LweI GCATC 5 cut(s) 196, 229, 406, 681, 1250
MabI ACCWGGT 1 cut(s) 982
MaeI CTAG 2 cut(s) 260, 1436
MaeII ACGT 3 cut(s) 780, 840, 1026
MaeIII GTNAC 2 cut(s) 1187, 1276
MalI GATC 9 cut(s) 219, 389, 428, 528, 777, 814, 854, 1125, 1256
MboI GATC 9 cut(s) 217, 387, 426, 526, 775, 812, 852, 1123, 1254
MboII GAAGA 2 cut(s) 482, 806
MflI RGATCY 2 cut(s) 812, 852
MhlI GDGCHC 2 cut(s) 345, 417
MlsI TGGCCA 2 cut(s) 479, 1073
MluCI AATT 7 cut(s) 63, 213, 580, 818, 1063, 1393, 1400
MluNI TGGCCA 2 cut(s) 479, 1073
MlyI GAGTC 2 cut(s) 1259, 1363
MmeI TCCRAC 1 cut(s) 468
Mox20I TGGCCA 2 cut(s) 479, 1073
MscI TGGCCA 2 cut(s) 479, 1073
MseI TTAA 1 cut(s) 1462
MslI CAYNNNNRTG 2 cut(s) 350, 968
Msp20I TGGCCA 2 cut(s) 479, 1073
MspA1I CMGCKG 1 cut(s) 1222
MspI CCGG 5 cut(s) 143, 243, 467, 684, 1274
MspR9I CCNGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
Mva1269I GAATGC 3 cut(s) 275, 948, 1133
MvaI CCWGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
MvnI CGCG 2 cut(s) 422, 620
NcoI CCATGG 1 cut(s) 448
NdeI CATATG 1 cut(s) 938
NdeII GATC 9 cut(s) 217, 387, 426, 526, 775, 812, 852, 1123, 1254
NlaIV GGNNCC 3 cut(s) 302, 523, 770
NmuCI GTSAC 1 cut(s) 1276
NspI RCATGY 3 cut(s) 362, 645, 1284
OliI CACNNNNGTG 2 cut(s) 350, 968
PaeI GCATGC 2 cut(s) 362, 645
PagI TCATGA 1 cut(s) 712
PasI CCCWGGG 1 cut(s) 1008
PcsI WCGNNNNNNNCGW 1 cut(s) 13
PctI GAATGC 3 cut(s) 275, 948, 1133
PfeI GAWTC 5 cut(s) 4, 182, 440, 1225, 1324
PflMI CCANNNNNTGG 2 cut(s) 369, 1439
PfoI TCCNGGA 1 cut(s) 1335
PinAI ACCGGT 1 cut(s) 1273
PleI GAGTC 2 cut(s) 1259, 1362
PpsI GAGTC 2 cut(s) 1259, 1362
Ppu21I YACGTR 1 cut(s) 1027
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 6 cut(s) 517, 652, 771, 982, 1007, 1335
PspGI CCWGG 6 cut(s) 517, 652, 771, 982, 1007, 1335
PspN4I GGNNCC 3 cut(s) 302, 523, 770
PspPI GGNCC 3 cut(s) 586, 906, 1430
PsrI GAACNNNNNNTAC 2 cut(s) 1172, 1204
PstI CTGCAG 1 cut(s) 162
PsuI RGATCY 2 cut(s) 812, 852
RsaI GTAC 3 cut(s) 10, 770, 839
RsaNI GTAC 3 cut(s) 9, 769, 838
RseI CAYNNNNRTG 2 cut(s) 350, 968
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 1 cut(s) 1462
Sau3AI GATC 9 cut(s) 217, 387, 426, 526, 775, 812, 852, 1123, 1254
Sau96I GGNCC 3 cut(s) 586, 906, 1430
SchI GAGTC 2 cut(s) 1259, 1363
ScrFI CCNGG 6 cut(s) 519, 654, 773, 984, 1009, 1337
SduI GDGCHC 2 cut(s) 345, 417
SexAI ACCWGGT 1 cut(s) 982
SfaNI GCATC 5 cut(s) 196, 229, 406, 681, 1250
SfcI CTRYAG 1 cut(s) 158
SgrAI CRCCGGYG 1 cut(s) 1273
SinI GGWCC 2 cut(s) 586, 1430
SmiMI CAYNNNNRTG 2 cut(s) 350, 968
SmlI CTYRAG 2 cut(s) 254, 326
SmoI CTYRAG 2 cut(s) 254, 326
SnaBI TACGTA 1 cut(s) 1027
SpeI ACTAGT 1 cut(s) 1435
SphI GCATGC 2 cut(s) 362, 645
Sse9I AATT 7 cut(s) 63, 213, 580, 818, 1063, 1393, 1400
SsiI CCGC 5 cut(s) 492, 558, 973, 1035, 1222
SspMI CTAG 2 cut(s) 260, 1436
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 6 cut(s) 517, 652, 771, 982, 1007, 1335
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 1 cut(s) 1097
TaiI ACGT 3 cut(s) 783, 843, 1029
TaqI TCGA 2 cut(s) 1253, 1268
TasI AATT 7 cut(s) 63, 213, 580, 818, 1063, 1393, 1400
TauI GCSGC 2 cut(s) 560, 976
TfiI GAWTC 5 cut(s) 4, 182, 440, 1225, 1324
Tru1I TTAA 1 cut(s) 1462
Tru9I TTAA 1 cut(s) 1462
TscAI CASTG 1 cut(s) 1326
TseFI GTSAC 1 cut(s) 1276
Tsp45I GTSAC 1 cut(s) 1276
TspDTI ATGAA 7 cut(s) 17, 47, 732, 774, 925, 1217, 1377
TspGWI ACGGA 2 cut(s) 392, 813
TspRI CASTG 1 cut(s) 1326
Van91I CCANNNNNTGG 2 cut(s) 369, 1439
VpaK11BI GGWCC 2 cut(s) 586, 1430
XapI RAATTY 2 cut(s) 580, 818
XceI RCATGY 3 cut(s) 362, 645, 1284
XmiI GTMKAC 1 cut(s) 693
XspI CTAG 2 cut(s) 260, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.