MD02G1228000.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003358
Physical Location & Seq
Forward (+)
26979311 .. 26981661
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1228000.v1.1.491

Sequence Viewer

Length: 1473 bp
ATGGCTTCCCCCAATTTATTTGGTTTGATTTGCCTTCTTATGTTTTTATTGACTACTCAAAATGCAGTTCTTGGTGATATAGACTATGGCGATGCTCTCACCAAGTCTTTGTTGTTTTACGAGGCTCAACGTTCCGGTAAATTGCCTTCCACGCAAAGAGTGCAATGGCGTGGAGATTCTGGGCTTAAAGATGGAAGTGATGCTGGTGTTGATCTTGTGGGAGGATATTATGATGCTGGTGACAATGTTAAGTTTGGATTTCCCATGGCATTCACAATAACAATGCTTTCATGGAGTACATTGGAGTTCACCAAACAACTCGAGGCCAAGGATGAGCTGTCAAATGCATTGGATGCCATCAAATGGGGAACGGATTATCTGATCAAAGCACACACTACGCCGAATGTCCTCTATGGTGAAGTTGGTGATGGTGATTCAGATCATGAGTGCTGGCAGAGACCCGAAGACATGACCACTCCGCGAACAACCTTTAAGATTGATGAGCAACACCCAGGATCTGACCTTGCCGGTGAAACTGCTGCTGCTTTGGCTGCAGCTTCCATTGCTTTCAAGGATAAAAACCCTAAATATGCATCCGACCTTTTGACGCATGCAAAACAATTATTCGAGTTTGCTCGCAATCACCCTGGCATTTATTCGAATAGCATTTCAGCAGCAGGAAAATTCTATGCCAGCAGTGGATATGAGGATGAGCTATTATGGGCTGCTTCATGGCTTCATCGTGCTACTGATGATAAAACCTACCTGGATAATCTTGGCCAAGCAGGCAATACTGGTGGTGCTAGAACTGTGTTTGCTTGGGATGACAAGTTTATTGGCGCACAAGTCCTTGTTGCAAAGCTTGTTTTGGAGGGCAAGGTAGACTCTGCAGGTGCATGGGGACAATACAAGAGCCAAGCTGAGCAATTCATTTGCTCTTGCATTCAAAAAGGTAACAACAATGTGAAGAAGACGCCTGGTGGGTTGTTATGGTTTTTGCCATGGAACAATCTTCAGTACACTTCAACTGCTGTATTTGTCGCAACCGCATATTCAGAGTACTTGAACGCCAAGCATGCCTCCATTCAATGCCCTGGGGGCATTGTCAAGCCGTCTGATCTTATTGCCTTGGCCCAGTCACAGGTGGACTATATCTTGGGATCGAATCCAGAGGGTATGAGTTTCATGGTTGGATTTGGGACAAAATATCCGACCCAAGTTCACCACAGAGGAGCATCCATTGTTTCAATCAAGGAGAATTCAGCTCCTGTTTCATGCAAAGCTGGGTTCGACTTGTGGTTCAACAAGACCTCGCCAAACCCGAATGTATTGGATGGAGCAATCGTTGGTGGCCCGGATGAGAATGATGGCTACTCAGATTCAAGGTCGAACTTCCAAATGGCTGAACCAGCAACTGTTTCTACAGCACCACTAGTAGGTGTCCTCGCTAGGCTTGCCTTAGTTAATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

491

Amino Acids

53.09

Weight (kDa)

5.12

Isoelectric Point (pI)

25.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 29 - 482 1.2e-166 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 881
Acc36I ACCTGC 1 cut(s) 881
AccB7I CCANNNNNTGG 1 cut(s) 363
AccI GTMKAC 1 cut(s) 882
AccII CGCG 1 cut(s) 481
AciI CCGC 2 cut(s) 479, 1047
AclI AACGTT 1 cut(s) 130
AclWI GGATC 2 cut(s) 523, 1168
AcoI YGGCCR 1 cut(s) 778
AcsI RAATTY 2 cut(s) 683, 1258
AcuI CTGAAG 1 cut(s) 998
AcyI GRCGYC 1 cut(s) 974
AfaI GTAC 3 cut(s) 298, 1019, 1061
AfiI CCNNNNNNNGG 3 cut(s) 363, 1141, 1436
AgsI TTSAA 8 cut(s) 571, 947, 1026, 1066, 1088, 1248, 1303, 1383
AhlI ACTAGT 1 cut(s) 1432
AjnI CCWGG 5 cut(s) 511, 646, 765, 976, 1093
AluBI AGCT 7 cut(s) 337, 557, 715, 862, 920, 1265, 1283
AluI AGCT 7 cut(s) 337, 557, 715, 862, 920, 1265, 1283
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 2 cut(s) 523, 1168
AlwNI CAGNNNCTG 3 cut(s) 518, 1268, 1415
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 4 cut(s) 324, 778, 1131, 1351
ApeKI GCWGC 6 cut(s) 539, 542, 551, 554, 674, 725
ApoI RAATTY 2 cut(s) 683, 1258
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 2 cut(s) 1132, 1352
AsuC2I CCSGG 1 cut(s) 1355
AsuII TTCGAA 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 320
BalI TGGCCA 1 cut(s) 780
BarI GAAGNNNNNNTAC 2 cut(s) 130, 162
BbsI GAAGAC 2 cut(s) 471, 977
BbvI GCAGC 6 cut(s) 526, 529, 538, 566, 686, 712
BccI CCATC 5 cut(s) 185, 365, 422, 1328, 1361
BceAI ACGGC 1 cut(s) 1096
BciT130I CCWGG 5 cut(s) 513, 648, 767, 978, 1095
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 1 cut(s) 1355
BcoDI GTCTC 1 cut(s) 451
BcuI ACTAGT 1 cut(s) 1432
BfaI CTAG 3 cut(s) 804, 1433, 1449
BfmI CTRYAG 3 cut(s) 552, 888, 1422
BfuAI ACCTGC 1 cut(s) 881
BglI GCCNNNNNGGC 2 cut(s) 786, 1098
BisI GCNGC 6 cut(s) 540, 543, 552, 555, 675, 726
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 6 cut(s) 541, 544, 553, 556, 676, 727
BmcAI AGTACT 1 cut(s) 1061
Bme1390I CCNGG 6 cut(s) 513, 648, 767, 978, 1095, 1355
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 2 cut(s) 1132, 1352
BmrFI CCNGG 6 cut(s) 513, 648, 767, 978, 1095, 1355
BmrI ACTGGG 1 cut(s) 1129
BmsI GCATC 6 cut(s) 82, 190, 223, 343, 602, 1244
BmuI ACTGGG 1 cut(s) 1129
BpiI GAAGAC 2 cut(s) 471, 977
Bpu1102I GCTNAGC 1 cut(s) 921
Bpu14I TTCGAA 1 cut(s) 659
BpuMI CCSGG 1 cut(s) 1355
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 974
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 8 cut(s) 264, 327, 511, 646, 1001, 1093, 1094, 1128
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 1329, 1359
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 1141, 1436
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 2 cut(s) 799, 1135
Bse3DI GCAATG 2 cut(s) 170, 561
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 5 cut(s) 513, 648, 767, 978, 1095
BseDI CCNNGG 8 cut(s) 264, 327, 511, 646, 1001, 1093, 1094, 1128
BseGI GGATG 8 cut(s) 337, 358, 593, 715, 829, 1235, 1339, 1363
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 3 cut(s) 363, 1141, 1436
BseMI GCAATG 2 cut(s) 170, 561
BseMII CTCAG 2 cut(s) 912, 1389
BseNI ACTGG 2 cut(s) 799, 1135
BseRI GAGGAG 1 cut(s) 1245
BseXI GCAGC 6 cut(s) 526, 529, 538, 566, 686, 712
BseYI CCCAGC 1 cut(s) 1283
Bsh1236I CGCG 1 cut(s) 481
BshFI GGCC 4 cut(s) 326, 780, 1133, 1353
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 3 cut(s) 135, 528, 1355
BslFI GGGAC 2 cut(s) 915, 1213
BslI CCNNNNNNNGG 3 cut(s) 363, 1141, 1436
BsmAI GTCTC 1 cut(s) 451
BsmFI GGGAC 2 cut(s) 915, 1213
BsmI GAATGC 2 cut(s) 269, 942
BsnI GGCC 4 cut(s) 326, 780, 1133, 1353
Bso31I GGTCTC 1 cut(s) 451
BsoBI CYCGRG 1 cut(s) 320
Bsp119I TTCGAA 1 cut(s) 659
Bsp143I GATC 6 cut(s) 211, 381, 439, 515, 1117, 1160
Bsp1720I GCTNAGC 1 cut(s) 921
Bsp19I CCATGG 2 cut(s) 264, 1001
BspACI CCGC 2 cut(s) 479, 1047
BspANI GGCC 4 cut(s) 326, 780, 1133, 1353
BspCNI CTCAG 2 cut(s) 913, 1388
BspFNI CGCG 1 cut(s) 481
BspHI TCATGA 1 cut(s) 442
BspMAI CTGCAG 2 cut(s) 556, 892
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 2 cut(s) 523, 1168
BspT104I TTCGAA 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 451
BsrDI GCAATG 2 cut(s) 170, 561
BsrFI RCCGGY 1 cut(s) 527
BsrI ACTGG 2 cut(s) 799, 1135
BssAI RCCGGY 1 cut(s) 527
BssECI CCNNGG 8 cut(s) 264, 327, 511, 646, 1001, 1093, 1094, 1128
BssMI GATC 6 cut(s) 211, 381, 439, 515, 1117, 1160
BssNI GRCGYC 1 cut(s) 974
BssT1I CCWWGG 4 cut(s) 264, 327, 1001, 1128
Bst2UI CCWGG 5 cut(s) 513, 648, 767, 978, 1095
Bst4CI ACNGT 2 cut(s) 811, 1417
BstACI GRCGYC 1 cut(s) 974
BstAPI GCANNNNNTGC 2 cut(s) 160, 353
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 7 cut(s) 452, 612, 637, 694, 787, 1077, 1455
BstDEI CTNAG 3 cut(s) 921, 1375, 1459
BstDSI CCRYGG 2 cut(s) 264, 1001
BstF5I GGATG 8 cut(s) 337, 358, 593, 715, 829, 1235, 1339, 1363
BstFNI CGCG 1 cut(s) 481
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 6 cut(s) 214, 384, 442, 518, 1120, 1163
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 6 cut(s) 211, 381, 439, 515, 1117, 1160
BstNI CCWGG 5 cut(s) 513, 648, 767, 978, 1095
BstNSI RCATGY 2 cut(s) 614, 1079
BstSCI CCNGG 6 cut(s) 511, 646, 765, 976, 1093, 1353
BstSFI CTRYAG 3 cut(s) 552, 888, 1422
BstUI CGCG 1 cut(s) 481
BstV1I GCAGC 6 cut(s) 526, 529, 538, 566, 686, 712
BstV2I GAAGAC 2 cut(s) 471, 977
BstX2I RGATCY 1 cut(s) 515
BstYI RGATCY 1 cut(s) 515
BsuRI GGCC 4 cut(s) 326, 780, 1133, 1353
BtgI CCRYGG 2 cut(s) 264, 1001
BtgZI GCGATG 1 cut(s) 105
BtsCI GGATG 8 cut(s) 337, 358, 593, 715, 829, 1235, 1339, 1363
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 1 cut(s) 703
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 7 cut(s) 452, 612, 637, 694, 787, 1077, 1455
CaiI CAGNNNCTG 3 cut(s) 518, 1268, 1415
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 842
Cfr10I RCCGGY 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 1132, 1352
CseI GACGC 2 cut(s) 616, 982
Csp6I GTAC 3 cut(s) 297, 1018, 1060
CspCI CAANNNNNGTGG 2 cut(s) 778, 813
CviQI GTAC 3 cut(s) 297, 1018, 1060
DdeI CTNAG 3 cut(s) 921, 1375, 1459
DpnI GATC 6 cut(s) 213, 383, 441, 517, 1119, 1162
DpnII GATC 6 cut(s) 211, 381, 439, 515, 1117, 1160
EaeI YGGCCR 1 cut(s) 778
Eco130I CCWWGG 4 cut(s) 264, 327, 1001, 1128
Eco31I GGTCTC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 998
Eco88I CYCGRG 1 cut(s) 320
EcoRI GAATTC 1 cut(s) 1258
EcoRII CCWGG 5 cut(s) 511, 646, 765, 976, 1093
EcoT14I CCWWGG 4 cut(s) 264, 327, 1001, 1128
EcoT22I ATGCAT 2 cut(s) 349, 595
ErhI CCWWGG 4 cut(s) 264, 327, 1001, 1128
FaqI GGGAC 2 cut(s) 915, 1213
FbaI TGATCA 1 cut(s) 381
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 6 cut(s) 540, 543, 552, 555, 675, 726
FokI GGATG 8 cut(s) 344, 365, 580, 722, 836, 1222, 1346, 1370
Fsp4HI GCNGC 6 cut(s) 540, 543, 552, 555, 675, 726
FspBI CTAG 3 cut(s) 804, 1433, 1449
GlaI GCGC 1 cut(s) 841
GluI GCNGC 6 cut(s) 540, 543, 552, 555, 675, 726
GsaI CCCAGC 1 cut(s) 1287
HaeIII GGCC 4 cut(s) 326, 780, 1133, 1353
HapII CCGG 3 cut(s) 135, 528, 1355
HgaI GACGC 2 cut(s) 616, 982
HhaI GCGC 1 cut(s) 842
Hin1I GRCGYC 1 cut(s) 974
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 5 cut(s) 176, 434, 884, 1165, 1379
HpaII CCGG 3 cut(s) 135, 528, 1355
Hpy166II GTNNAC 5 cut(s) 309, 883, 1020, 1147, 1222
Hpy188I TCNGA 8 cut(s) 381, 439, 520, 598, 1057, 1117, 1212, 1378
Hpy188III TCNNGA 2 cut(s) 443, 1169
Hpy8I GTNNAC 5 cut(s) 309, 883, 1020, 1147, 1222
HpyAV CCTTC 2 cut(s) 44, 156
HpyCH4III ACNGT 2 cut(s) 811, 1417
HpyCH4IV ACGT 1 cut(s) 130
HpyF3I CTNAG 3 cut(s) 921, 1375, 1459
HpySE526I ACGT 1 cut(s) 130
Hsp92I GRCGYC 1 cut(s) 974
HspAI GCGC 1 cut(s) 840
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 6 cut(s) 211, 381, 439, 515, 1117, 1160
LmnI GCTCC 3 cut(s) 1232, 1270, 1337
Lsp1109I GCAGC 6 cut(s) 526, 529, 538, 566, 686, 712
LweI GCATC 6 cut(s) 82, 190, 223, 343, 602, 1244
MaeI CTAG 3 cut(s) 804, 1433, 1449
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 3 cut(s) 239, 953, 1137
MalI GATC 6 cut(s) 213, 383, 441, 517, 1119, 1162
MboI GATC 6 cut(s) 211, 381, 439, 515, 1117, 1160
MboII GAAGA 4 cut(s) 476, 979, 982, 1004
MflI RGATCY 1 cut(s) 515
MlsI TGGCCA 1 cut(s) 780
MluCI AATT 7 cut(s) 13, 140, 620, 683, 926, 1258, 1465
MluNI TGGCCA 1 cut(s) 780
MlyI GAGTC 1 cut(s) 878
MmeI TCCRAC 3 cut(s) 621, 1171, 1235
Mox20I TGGCCA 1 cut(s) 780
Mph1103I ATGCAT 2 cut(s) 349, 595
MscI TGGCCA 1 cut(s) 780
MseI TTAA 4 cut(s) 186, 249, 492, 1464
Msp20I TGGCCA 1 cut(s) 780
MspI CCGG 3 cut(s) 135, 528, 1355
MspR9I CCNGG 6 cut(s) 513, 648, 767, 978, 1095, 1355
Mva1269I GAATGC 2 cut(s) 269, 942
MvaI CCWGG 5 cut(s) 513, 648, 767, 978, 1095
MvnI CGCG 1 cut(s) 481
NciI CCSGG 1 cut(s) 1355
NcoI CCATGG 2 cut(s) 264, 1001
NdeII GATC 6 cut(s) 211, 381, 439, 515, 1117, 1160
NmuCI GTSAC 2 cut(s) 239, 1137
NsiI ATGCAT 2 cut(s) 349, 595
NspI RCATGY 2 cut(s) 614, 1079
NspV TTCGAA 1 cut(s) 659
PaeI GCATGC 2 cut(s) 614, 1079
PaeR7I CTCGAG 1 cut(s) 320
PagI TCATGA 1 cut(s) 442
PaqCI CACCTGC 1 cut(s) 881
PasI CCCWGGG 1 cut(s) 1094
PcsI WCGNNNNNNNCGW 1 cut(s) 1319
PctI GAATGC 2 cut(s) 269, 942
PfeI GAWTC 4 cut(s) 176, 434, 1165, 1379
PflMI CCANNNNNTGG 1 cut(s) 363
PkrI GCNGC 6 cut(s) 541, 544, 553, 556, 676, 727
PleI GAGTC 1 cut(s) 878
PpsI GAGTC 1 cut(s) 878
Psp1406I AACGTT 1 cut(s) 130
Psp6I CCWGG 5 cut(s) 511, 646, 765, 976, 1093
PspFI CCCAGC 1 cut(s) 1283
PspGI CCWGG 5 cut(s) 511, 646, 765, 976, 1093
PspPI GGNCC 2 cut(s) 1132, 1352
PspXI VCTCGAGB 1 cut(s) 320
PstI CTGCAG 2 cut(s) 556, 892
PstNI CAGNNNCTG 3 cut(s) 518, 1268, 1415
PsuI RGATCY 1 cut(s) 515
RsaI GTAC 3 cut(s) 298, 1019, 1061
RsaNI GTAC 3 cut(s) 297, 1018, 1060
SaqAI TTAA 4 cut(s) 186, 249, 492, 1464
SatI GCNGC 6 cut(s) 540, 543, 552, 555, 675, 726
Sau3AI GATC 6 cut(s) 211, 381, 439, 515, 1117, 1160
Sau96I GGNCC 2 cut(s) 1132, 1352
ScaI AGTACT 1 cut(s) 1061
SchI GAGTC 1 cut(s) 878
ScrFI CCNGG 6 cut(s) 513, 648, 767, 978, 1095, 1355
SfaNI GCATC 6 cut(s) 82, 190, 223, 343, 602, 1244
SfcI CTRYAG 3 cut(s) 552, 888, 1422
Sfr274I CTCGAG 1 cut(s) 320
SfuI TTCGAA 1 cut(s) 659
SlaI CTCGAG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SpeI ACTAGT 1 cut(s) 1432
SphI GCATGC 2 cut(s) 614, 1079
Sse9I AATT 7 cut(s) 13, 140, 620, 683, 926, 1258, 1465
SsiI CCGC 2 cut(s) 479, 1047
SspMI CTAG 3 cut(s) 804, 1433, 1449
StyD4I CCNGG 6 cut(s) 511, 646, 765, 976, 1093, 1353
StyI CCWWGG 4 cut(s) 264, 327, 1001, 1128
TaaI ACNGT 2 cut(s) 811, 1417
TaiI ACGT 1 cut(s) 133
TaqI TCGA 6 cut(s) 321, 627, 659, 1163, 1290, 1388
TasI AATT 7 cut(s) 13, 140, 620, 683, 926, 1258, 1465
TatI WGTACW 3 cut(s) 296, 1017, 1059
TfiI GAWTC 4 cut(s) 176, 434, 1165, 1379
Tru1I TTAA 4 cut(s) 186, 249, 492, 1464
Tru9I TTAA 4 cut(s) 186, 249, 492, 1464
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 2 cut(s) 239, 1137
TseI GCWGC 6 cut(s) 539, 542, 551, 554, 674, 725
Tsp45I GTSAC 2 cut(s) 239, 1137
TspDTI ATGAA 6 cut(s) 279, 720, 728, 919, 1174, 1263
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 1 cut(s) 703
Van91I CCANNNNNTGG 1 cut(s) 363
XapI RAATTY 2 cut(s) 683, 1258
XceI RCATGY 2 cut(s) 614, 1079
XhoI CTCGAG 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 882
XspI CTAG 3 cut(s) 804, 1433, 1449
ZrmI AGTACT 1 cut(s) 1061
Zsp2I ATGCAT 2 cut(s) 349, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.