Rh1AG179800

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003358
Physical Location & Seq
Reverse (-)
34110180 .. 34125893
15714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG179800.1

Sequence Viewer

Length: 1380 bp
ATGAACAGTGCGAGGAACAGCGATTCGGCTTTAGATGCTCAACGATCTGGTAAGTTGCCTCCTGAACAGAGAGTGCAATGGCGTGGCGATTCAGCGCTTAAAGATGGAAATGATACTGGTGTTAATCTTGATGGAGGGTATTATGATTCTGGGGACAATGTCAAGTTTGGATTCCCCATGGCATTCACAATAACAATGCTTTCATGGAGCACAATAGAGTTCGCAAGCAAACTTGAAACCAAGAATGAGCTCTCAAACGCATTGGACGCCATTAAATGGGGCACAGACTATTTGATCAAAGCACACGCTCAGCCAAATGTTCTCTATGGTGAAGTTGGCGATGGCGGTTCTGATCATGCTTGTTGGCAGAGACCGGAAGACATGACTACCCCACGGACATCCTTCAAGATCGATGAGCAGCATCCAGGTTCTGATCTTGCTGGTGAAACCGCAGCTGCTTTTGCCGCAGCTTCCATTGCTTTCAAGGATAAAGACCCTACATATTCTGGCCAGCTCTTGATGCATGCGAAACAGCTGTTTGAATTTGCCTGCAGTCATGCTGGTGTTTATCAGAATAGTATTAATGTAGCAGGCCAGTTCTACTCCAGCAGCGGATATGAGGATGAATTATTGTGGGCTTCAGCATGGCTTCATCGTGCTACTGATGATAAATCGTACCTAGATTACCTTGGTCATCAAGCAGGCAATACTGGTGGTGCAAGAACCGAGTTCTCTTGGGATGACAAGTTTGTTGGCGCCCAGGTCTTGGTTGCAAAGCTTGTCTTGGAGGGCAAGGTACCGGCTGATTCAGGACCGTGGTCACAATACAAAAACCAGGCAGAGCAGTTCATTTGCTCTTGCATTCAGAAGGGTAACAGCAACGTGAAGAAGACGCCCGGTGGGTTGTTATGGTTTTTGCCATTTAACAATCTTCAGTATACTACAACTGCTACACTCATTGCAACTGTTTACTCTGAATACCTATCCCCAAAACGTGCCTCCATTCAATGTAGTGGTGGCATTGTTCAGGCTTCTGCTCTACTTGACTTGGCCCGATCACAGGTGGACTATATCCTGGGTTCAAATCCTAATGGTATGAGCTACATGGTTGGATTTGGGTCAAAGTATCCAACTCAAGTTCATCATAGAGGAGCATCCATTGTTTCAATCAAGAAAGATCCATCTCCAGTTGCATGCCAAGCTGGCTTCGACGCTTGGTTTAACATGAACGCCCCTAATCCGAATGTATTGGATGGAGCAATAGTTGGAGGACCCAATGATAGTGATGGTTATACGGATTCAAGATCCAACTTCCAAATGGCTGAAGCAGCAACTGTTAATACTGCACCACTAGTTGGTGTCCTAGCCAAGCTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

459

Amino Acids

49.58

Weight (kDa)

5.22

Isoelectric Point (pI)

30.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 11 - 455 7.9e-157 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 796
AccB1I GGYRCC 2 cut(s) 755, 796
AccB7I CCANNNNNTGG 3 cut(s) 276, 766, 1355
AccI GTMKAC 1 cut(s) 938
AciI CCGC 4 cut(s) 345, 450, 465, 612
AclWI GGATC 2 cut(s) 1172, 1299
AcoI YGGCCR 1 cut(s) 508
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 3 cut(s) 624, 917, 1344
AcyI GRCGYC 3 cut(s) 267, 756, 893
AfaI GTAC 2 cut(s) 677, 798
AfeI AGCGCT 1 cut(s) 96
AfiI CCNNNNNNNGG 4 cut(s) 276, 766, 1060, 1355
AgsI TTSAA 8 cut(s) 236, 406, 484, 542, 1007, 1083, 1167, 1302
AhlI ACTAGT 1 cut(s) 1351
AjnI CCWGG 4 cut(s) 424, 759, 834, 1074
AluBI AGCT 9 cut(s) 250, 455, 470, 514, 535, 778, 1101, 1202, 1372
AluI AGCT 9 cut(s) 250, 455, 470, 514, 535, 778, 1101, 1202, 1372
Alw21I GWGCWC 2 cut(s) 212, 252
Alw26I GTCTC 1 cut(s) 364
AlwI GGATC 2 cut(s) 1172, 1299
AlwNI CAGNNNCTG 2 cut(s) 431, 1334
Aor51HI AGCGCT 1 cut(s) 96
AoxI GGCC 3 cut(s) 508, 592, 1050
ApeKI GCWGC 6 cut(s) 418, 452, 455, 467, 609, 1328
ApoI RAATTY 1 cut(s) 542
AseI ATTAAT 1 cut(s) 582
Asp718I GGTACC 1 cut(s) 796
AspLEI GCGC 2 cut(s) 97, 758
AspS9I GGNCC 3 cut(s) 812, 1051, 1271
AsuC2I CCSGG 1 cut(s) 897
AsuHPI GGTGA 2 cut(s) 341, 455
AvaII GGWCC 2 cut(s) 812, 1271
BaeGI GKGCMC 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 105, 138
BalI TGGCCA 1 cut(s) 510
BanI GGYRCC 2 cut(s) 755, 796
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 2 cut(s) 384, 896
Bbv12I GWGCWC 2 cut(s) 212, 252
BbvI GCAGC 6 cut(s) 430, 442, 464, 479, 621, 1340
BccI CCATC 6 cut(s) 98, 125, 335, 1189, 1247, 1280
BciT130I CCWGG 4 cut(s) 426, 761, 836, 1076
BciVI GTATCC 1 cut(s) 1137
BclI TGATCA 2 cut(s) 294, 352
BcnI CCSGG 1 cut(s) 897
BcoDI GTCTC 1 cut(s) 364
BcuI ACTAGT 1 cut(s) 1351
BfaI CTAG 3 cut(s) 680, 1352, 1364
BfmI CTRYAG 1 cut(s) 550
BfoI RGCGCY 2 cut(s) 98, 759
BfuI GTATCC 1 cut(s) 1137
BglI GCCNNNNNGGC 1 cut(s) 1203
BisI GCNGC 7 cut(s) 419, 453, 456, 465, 468, 610, 1329
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 7 cut(s) 420, 454, 457, 466, 469, 611, 1330
Bme1390I CCNGG 5 cut(s) 426, 761, 836, 897, 1076
Bme18I GGWCC 2 cut(s) 812, 1271
BmgT120I GGNCC 3 cut(s) 812, 1051, 1271
BmiI GGNNCC 3 cut(s) 757, 798, 1273
BmrFI CCNGG 5 cut(s) 426, 761, 836, 897, 1076
BmsI GCATC 4 cut(s) 25, 430, 510, 1163
BoxI GACNNNNGTC 1 cut(s) 817
BpiI GAAGAC 2 cut(s) 384, 896
BpmI CTGGAG 2 cut(s) 589, 1170
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 1119
BpuMI CCSGG 1 cut(s) 897
Bsa29I ATCGAT 1 cut(s) 411
BsaHI GRCGYC 3 cut(s) 267, 756, 893
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 6 cut(s) 177, 392, 688, 759, 815, 1075
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 2 cut(s) 1248, 1278
Bsc4I CCNNNNNNNGG 4 cut(s) 276, 766, 1060, 1355
Bse118I RCCGGY 1 cut(s) 799
Bse1I ACTGG 4 cut(s) 121, 595, 715, 1187
Bse3DI GCAATG 3 cut(s) 83, 474, 957
BseBI CCWGG 4 cut(s) 426, 761, 836, 1076
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 6 cut(s) 177, 392, 688, 759, 815, 1075
BseGI GGATG 6 cut(s) 398, 421, 628, 745, 1154, 1258
BseLI CCNNNNNNNGG 4 cut(s) 276, 766, 1060, 1355
BseMI GCAATG 3 cut(s) 83, 474, 957
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 4 cut(s) 121, 595, 715, 1187
BseRI GAGGAG 1 cut(s) 1164
BseSI GKGCMC 1 cut(s) 284
BseXI GCAGC 6 cut(s) 430, 442, 464, 479, 621, 1340
BsgI GTGCAG 1 cut(s) 1329
BshFI GGCC 3 cut(s) 510, 594, 1052
BshNI GGYRCC 2 cut(s) 755, 796
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 2 cut(s) 212, 252
BsiSI CCGG 3 cut(s) 374, 800, 897
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 4 cut(s) 276, 766, 1060, 1355
BsmAI GTCTC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 2 cut(s) 182, 861
BsnI GGCC 3 cut(s) 510, 594, 1052
Bso31I GGTCTC 1 cut(s) 364
Bsp1286I GDGCHC 3 cut(s) 212, 252, 284
Bsp143I GATC 8 cut(s) 44, 294, 352, 408, 433, 1055, 1177, 1304
Bsp1720I GCTNAGC 1 cut(s) 309
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 4 cut(s) 345, 450, 465, 612
BspANI GGCC 3 cut(s) 510, 594, 1052
BspCNI CTCAG 1 cut(s) 322
BspDI ATCGAT 1 cut(s) 411
BspLI GGNNCC 3 cut(s) 757, 798, 1273
BspMAI CTGCAG 1 cut(s) 554
BspPI GGATC 2 cut(s) 1172, 1299
BspT107I GGYRCC 2 cut(s) 755, 796
BspTNI GGTCTC 1 cut(s) 364
BsrDI GCAATG 3 cut(s) 83, 474, 957
BsrFI RCCGGY 1 cut(s) 799
BsrI ACTGG 4 cut(s) 121, 595, 715, 1187
BssAI RCCGGY 1 cut(s) 799
BssECI CCNNGG 6 cut(s) 177, 392, 688, 759, 815, 1075
BssMI GATC 8 cut(s) 44, 294, 352, 408, 433, 1055, 1177, 1304
BssNAI GTATAC 1 cut(s) 939
BssNI GRCGYC 3 cut(s) 267, 756, 893
BssT1I CCWWGG 2 cut(s) 177, 688
Bst1107I GTATAC 1 cut(s) 939
Bst2UI CCWGG 4 cut(s) 426, 761, 836, 1076
Bst4CI ACNGT 4 cut(s) 8, 816, 967, 1336
BstACI GRCGYC 3 cut(s) 267, 756, 893
BstC8I GCNNGC 9 cut(s) 226, 512, 525, 550, 592, 703, 1195, 1204, 1374
BstDEI CTNAG 1 cut(s) 309
BstDSI CCRYGG 3 cut(s) 177, 392, 815
BstF5I GGATG 6 cut(s) 398, 421, 628, 745, 1154, 1258
BstH2I RGCGCY 2 cut(s) 98, 759
BstHHI GCGC 2 cut(s) 97, 758
BstKTI GATC 8 cut(s) 47, 297, 355, 411, 436, 1058, 1180, 1307
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 8 cut(s) 44, 294, 352, 408, 433, 1055, 1177, 1304
BstNI CCWGG 4 cut(s) 426, 761, 836, 1076
BstNSI RCATGY 2 cut(s) 527, 1197
BstPAI GACNNNNGTC 1 cut(s) 817
BstSCI CCNGG 5 cut(s) 424, 759, 834, 895, 1074
BstSFI CTRYAG 1 cut(s) 550
BstSLI GKGCMC 1 cut(s) 284
BstV1I GCAGC 6 cut(s) 430, 442, 464, 479, 621, 1340
BstV2I GAAGAC 2 cut(s) 384, 896
BstX2I RGATCY 2 cut(s) 1177, 1304
BstYI RGATCY 2 cut(s) 1177, 1304
BstZ17I GTATAC 1 cut(s) 939
Bsu15I ATCGAT 1 cut(s) 411
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 3 cut(s) 510, 594, 1052
BsuTUI ATCGAT 1 cut(s) 411
BtgI CCRYGG 3 cut(s) 177, 392, 815
BtgZI GCGATG 1 cut(s) 354
BtsCI GGATG 6 cut(s) 398, 421, 628, 745, 1154, 1258
BtsIMutI CAGTG 1 cut(s) 13
Cac8I GCNNGC 9 cut(s) 226, 512, 525, 550, 592, 703, 1195, 1204, 1374
CaiI CAGNNNCTG 2 cut(s) 431, 1334
CfoI GCGC 2 cut(s) 97, 758
Cfr10I RCCGGY 1 cut(s) 799
Cfr13I GGNCC 3 cut(s) 812, 1051, 1271
ClaI ATCGAT 1 cut(s) 411
CseI GACGC 3 cut(s) 275, 901, 1220
Csp6I GTAC 2 cut(s) 676, 797
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviQI GTAC 2 cut(s) 676, 797
DdeI CTNAG 1 cut(s) 309
DinI GGCGCC 1 cut(s) 757
DpnI GATC 8 cut(s) 46, 296, 354, 410, 435, 1057, 1179, 1306
DpnII GATC 8 cut(s) 44, 294, 352, 408, 433, 1055, 1177, 1304
EaeI YGGCCR 1 cut(s) 508
Ecl136II GAGCTC 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 177, 688
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 812, 1271
Eco47III AGCGCT 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 3 cut(s) 624, 917, 1344
EcoICRI GAGCTC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 1271
EcoRII CCWGG 4 cut(s) 424, 759, 834, 1074
EcoT14I CCWWGG 2 cut(s) 177, 688
EcoT22I ATGCAT 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 252
EgeI GGCGCC 1 cut(s) 757
EheI GGCGCC 1 cut(s) 757
ErhI CCWWGG 2 cut(s) 177, 688
FalI AAGNNNNNCTT 2 cut(s) 767, 799
FaqI GGGAC 1 cut(s) 167
FbaI TGATCA 2 cut(s) 294, 352
FblI GTMKAC 1 cut(s) 938
Fnu4HI GCNGC 7 cut(s) 419, 453, 456, 465, 468, 610, 1329
FokI GGATG 6 cut(s) 385, 408, 635, 752, 1141, 1265
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 7 cut(s) 419, 453, 456, 465, 468, 610, 1329
FspBI CTAG 3 cut(s) 680, 1352, 1364
GlaI GCGC 2 cut(s) 96, 757
GluI GCNGC 7 cut(s) 419, 453, 456, 465, 468, 610, 1329
GsuI CTGGAG 2 cut(s) 589, 1170
HaeII RGCGCY 2 cut(s) 98, 759
HaeIII GGCC 3 cut(s) 510, 594, 1052
HapII CCGG 3 cut(s) 374, 800, 897
HgaI GACGC 3 cut(s) 275, 901, 1220
HhaI GCGC 2 cut(s) 97, 758
Hin1I GRCGYC 3 cut(s) 267, 756, 893
Hin6I GCGC 2 cut(s) 95, 756
HinP1I GCGC 2 cut(s) 95, 756
HindIII AAGCTT 2 cut(s) 776, 1370
HinfI GANTC 6 cut(s) 23, 89, 146, 171, 806, 1298
HpaII CCGG 3 cut(s) 374, 800, 897
HphI GGTGA 2 cut(s) 341, 455
Hpy166II GTNNAC 3 cut(s) 939, 970, 1066
Hpy188I TCNGA 6 cut(s) 352, 433, 573, 867, 976, 1242
Hpy188III TCNNGA 7 cut(s) 62, 128, 406, 517, 810, 1171, 1302
Hpy8I GTNNAC 3 cut(s) 939, 970, 1066
Hpy99I CGWCG 1 cut(s) 1214
HpyAV CCTTC 2 cut(s) 412, 862
HpyCH4III ACNGT 4 cut(s) 8, 816, 967, 1336
HpyCH4IV ACGT 2 cut(s) 882, 994
HpyCH4V TGCA 9 cut(s) 76, 523, 552, 719, 773, 861, 962, 1193, 1346
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 2 cut(s) 882, 994
Hsp92I GRCGYC 3 cut(s) 267, 756, 893
HspAI GCGC 2 cut(s) 95, 756
KasI GGCGCC 1 cut(s) 755
KpnI GGTACC 1 cut(s) 800
Ksp22I TGATCA 2 cut(s) 294, 352
Kzo9I GATC 8 cut(s) 44, 294, 352, 408, 433, 1055, 1177, 1304
LmnI GCTCC 3 cut(s) 207, 1151, 1256
Lsp1109I GCAGC 6 cut(s) 430, 442, 464, 479, 621, 1340
LweI GCATC 4 cut(s) 25, 430, 510, 1163
MaeI CTAG 3 cut(s) 680, 1352, 1364
MaeII ACGT 2 cut(s) 882, 994
MaeIII GTNAC 2 cut(s) 819, 872
MalI GATC 8 cut(s) 46, 296, 354, 410, 435, 1057, 1179, 1306
MboI GATC 8 cut(s) 44, 294, 352, 408, 433, 1055, 1177, 1304
MboII GAAGA 4 cut(s) 389, 898, 901, 923
MflI RGATCY 2 cut(s) 1177, 1304
MhlI GDGCHC 3 cut(s) 212, 252, 284
MlsI TGGCCA 1 cut(s) 510
MluCI AATT 2 cut(s) 542, 626
MluNI TGGCCA 1 cut(s) 510
Mly113I GGCGCC 1 cut(s) 756
MmeI TCCRAC 4 cut(s) 1090, 1154, 1246, 1332
MnlI CCTC 8 cut(s) 6, 69, 128, 613, 781, 1009, 1142, 1262
Mox20I TGGCCA 1 cut(s) 510
Mph1103I ATGCAT 1 cut(s) 525
MscI TGGCCA 1 cut(s) 510
MseI TTAA 7 cut(s) 99, 123, 273, 582, 924, 1221, 1338
MslI CAYNNNNRTG 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 510
MspA1I CMGCKG 3 cut(s) 455, 535, 612
MspI CCGG 3 cut(s) 374, 800, 897
MspR9I CCNGG 5 cut(s) 426, 761, 836, 897, 1076
Mva1269I GAATGC 2 cut(s) 182, 861
MvaI CCWGG 4 cut(s) 426, 761, 836, 1076
NarI GGCGCC 1 cut(s) 756
NciI CCSGG 1 cut(s) 897
NcoI CCATGG 1 cut(s) 177
NdeII GATC 8 cut(s) 44, 294, 352, 408, 433, 1055, 1177, 1304
NlaIV GGNNCC 3 cut(s) 757, 798, 1273
NmuCI GTSAC 1 cut(s) 819
NsiI ATGCAT 1 cut(s) 525
NspI RCATGY 2 cut(s) 527, 1197
PaeI GCATGC 2 cut(s) 527, 1197
PctI GAATGC 2 cut(s) 182, 861
PfeI GAWTC 6 cut(s) 23, 89, 146, 171, 806, 1298
PflFI GACNNNGTC 1 cut(s) 158
PflMI CCANNNNNTGG 3 cut(s) 276, 766, 1355
PkrI GCNGC 7 cut(s) 420, 454, 457, 466, 469, 611, 1330
PluTI GGCGCC 1 cut(s) 759
PpuMI RGGWCCY 1 cut(s) 1271
PshAI GACNNNNGTC 1 cut(s) 817
PshBI ATTAAT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 4 cut(s) 424, 759, 834, 1074
PspGI CCWGG 4 cut(s) 424, 759, 834, 1074
PspN4I GGNNCC 3 cut(s) 757, 798, 1273
PspPI GGNCC 3 cut(s) 812, 1051, 1271
PspPPI RGGWCCY 1 cut(s) 1271
PstI CTGCAG 1 cut(s) 554
PstNI CAGNNNCTG 2 cut(s) 431, 1334
PsuI RGATCY 2 cut(s) 1177, 1304
PsyI GACNNNGTC 1 cut(s) 158
PvuII CAGCTG 2 cut(s) 455, 535
RsaI GTAC 2 cut(s) 677, 798
RsaNI GTAC 2 cut(s) 676, 797
RseI CAYNNNNRTG 1 cut(s) 561
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 7 cut(s) 99, 123, 273, 582, 924, 1221, 1338
SatI GCNGC 7 cut(s) 419, 453, 456, 465, 468, 610, 1329
Sau3AI GATC 8 cut(s) 44, 294, 352, 408, 433, 1055, 1177, 1304
Sau96I GGNCC 3 cut(s) 812, 1051, 1271
ScrFI CCNGG 5 cut(s) 426, 761, 836, 897, 1076
SduI GDGCHC 3 cut(s) 212, 252, 284
SfaNI GCATC 4 cut(s) 25, 430, 510, 1163
SfcI CTRYAG 1 cut(s) 550
SfoI GGCGCC 1 cut(s) 757
SinI GGWCC 2 cut(s) 812, 1271
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 1134
SmoI CTYRAG 1 cut(s) 1134
SpeI ACTAGT 1 cut(s) 1351
SphI GCATGC 2 cut(s) 527, 1197
Sse9I AATT 2 cut(s) 542, 626
SsiI CCGC 4 cut(s) 345, 450, 465, 612
SspDI GGCGCC 1 cut(s) 755
SspMI CTAG 3 cut(s) 680, 1352, 1364
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 5 cut(s) 424, 759, 834, 895, 1074
StyI CCWWGG 2 cut(s) 177, 688
TaaI ACNGT 4 cut(s) 8, 816, 967, 1336
TaiI ACGT 2 cut(s) 885, 997
TaqI TCGA 2 cut(s) 411, 1209
TasI AATT 2 cut(s) 542, 626
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 6 cut(s) 23, 89, 146, 171, 806, 1298
Tru1I TTAA 7 cut(s) 99, 123, 273, 582, 924, 1221, 1338
Tru9I TTAA 7 cut(s) 99, 123, 273, 582, 924, 1221, 1338
TscAI CASTG 1 cut(s) 13
TseFI GTSAC 1 cut(s) 819
TseI GCWGC 6 cut(s) 418, 452, 455, 467, 609, 1328
Tsp45I GTSAC 1 cut(s) 819
TspDTI ATGAA 7 cut(s) 17, 192, 639, 641, 838, 1130, 1241
TspGWI ACGGA 2 cut(s) 409, 1310
TspRI CASTG 1 cut(s) 13
Tth111I GACNNNGTC 1 cut(s) 158
Van91I CCANNNNNTGG 3 cut(s) 276, 766, 1355
VpaK11BI GGWCC 2 cut(s) 812, 1271
VspI ATTAAT 1 cut(s) 582
XapI RAATTY 1 cut(s) 542
XceI RCATGY 2 cut(s) 527, 1197
XcmI CCANNNNNNNNNTGG 1 cut(s) 1315
XmiI GTMKAC 1 cut(s) 938
XspI CTAG 3 cut(s) 680, 1352, 1364
Zsp2I ATGCAT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.