Rh1DG181500

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003358
Physical Location & Seq
Reverse (-)
36559510 .. 36561512
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG181500.1

Sequence Viewer

Length: 1284 bp
ATGAAGTTTACTGCCTACATTATACAGGTTGATCTTGATGGAGGGTATTATGATGCTGGGGACAATGTTAAGTTTGGATTCCCCATGGCATTCACAATAACAATGCTTTCATGGAGCACAATAGAGTTCGCAAGCAAACTTGAGACCAAGAATGAGCTCTCCAACGCATTGGACGCCATTAAATGGGGAACAGACTATTTGATCAAAGCACACCCTCAGCCAAACGTGCTCTATGGCCAAGTTGGCGATGGCGGTTCTGATCATACTTGTTGGCAGAGACCGGAAGACATGACTACCCCACGGACATCCTTCAAGATCGATGAGCAGCATCCAGGTTCTGATCTTGCTGGTGAAACCGCAGCTGCTTTTGCCGCAGCTTCCATTGCTTTCAAGGATAAAGACCCTACATATTCTGGCCAGCTCTTGACGCATGCGAAACAGCTGTTTGAATTTGCCCGCAGTCATGCTGGTGTTTATCAGAATAGCATTAATGTAGCGGGCCAGTTCTACTCCAGCAGCGGATATGAGGATGAATTATTGTGGGCTGCAGCATGGCTTCATCGTGCTACTGATGATAAATCGTACCTAGATTACCTTGGTCAAGCAGGCAATACTGGTGGTGCAAGAACCGTGTTCTCTTGGGATGACAAGTTTGTTGGCGCCCAGGTCTTGGTTGCAAAGCTTGTTTTGGAGGGCAAGGTACCGGCTGATTCAGGACCGTGGTCACAATACAAGAACCAGGCAGAGCAGTTCATTTGCTCTTGCATTCAGAAGGGTAACAACAACGTGAAGAAGACGCCCGGTGGCTTGTTATGGTTTTTGCCATTTAACAATCTTCAGTATACTGCAGCTGCTACACTCATTGCAACTGTTTACTCTGAATACCTTTCCCCAAAACGTGCCTCCATTCAATGTAGTGGTGGCATTGTTCAGGCTCCTGCTCTACTTGACTTGGCCCGATCACAGGTGGACTATATCCTGGGTTCAAATCCTAATGGTATGAGCTACATGGTTGGATTTGGTTCAAAGTATCCAACTCAAGTTCATCATAGAGGAGCATCCATTGTTTCAATCAAGAAAGATCCATCTCCAGTTGCATGCCAAGCTGGCTTCGACGCTTGGTTTAACAAGAACACCCCTAATCCGAATGTATTGGATGGAGCAATAGTTGGAGGACCCAATGATAGTGATGGTTATACGGATTCGAGAACCAACTTCCAAATGGCTGAAGCAGCAACTGTTAATACTGCACCACTAGTTGGTGTCCTAGCCAAGCTTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

427

Amino Acids

46.26

Weight (kDa)

5.5

Isoelectric Point (pI)

25.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 9 - 423 3.6e-148 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 700
AccB1I GGYRCC 2 cut(s) 659, 700
AccB7I CCANNNNNTGG 3 cut(s) 183, 670, 1259
AccI GTMKAC 1 cut(s) 842
AciI CCGC 6 cut(s) 252, 357, 372, 457, 497, 519
AclWI GGATC 1 cut(s) 1076
AcoI YGGCCR 2 cut(s) 235, 415
AcsI RAATTY 1 cut(s) 449
AcuI CTGAAG 2 cut(s) 821, 1248
AcyI GRCGYC 3 cut(s) 174, 660, 797
AfaI GTAC 2 cut(s) 584, 702
AfiI CCNNNNNNNGG 4 cut(s) 183, 670, 964, 1259
AgsI TTSAA 7 cut(s) 313, 391, 449, 911, 987, 1026, 1071
AhlI ACTAGT 1 cut(s) 1255
AjnI CCWGG 4 cut(s) 331, 663, 738, 978
Alw21I GWGCWC 3 cut(s) 119, 159, 231
Alw26I GTCTC 2 cut(s) 137, 271
AlwI GGATC 1 cut(s) 1076
AlwNI CAGNNNCTG 2 cut(s) 338, 1238
AoxI GGCC 4 cut(s) 235, 415, 499, 954
ApoI RAATTY 1 cut(s) 449
AseI ATTAAT 1 cut(s) 489
Asp718I GGTACC 1 cut(s) 700
AspLEI GCGC 1 cut(s) 662
AspS9I GGNCC 4 cut(s) 499, 716, 955, 1175
AsuC2I CCSGG 1 cut(s) 801
AsuHPI GGTGA 1 cut(s) 362
AvaII GGWCC 2 cut(s) 716, 1175
BalI TGGCCA 2 cut(s) 237, 417
BanI GGYRCC 2 cut(s) 659, 700
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 2 cut(s) 291, 800
Bbv12I GWGCWC 3 cut(s) 119, 159, 231
BbvCI CCTCAGC 1 cut(s) 216
BccI CCATC 5 cut(s) 32, 242, 1093, 1151, 1184
BciT130I CCWGG 4 cut(s) 333, 665, 740, 980
BciVI GTATCC 1 cut(s) 1041
BclI TGATCA 2 cut(s) 201, 259
BcnI CCSGG 1 cut(s) 801
BcoDI GTCTC 2 cut(s) 137, 271
BcuI ACTAGT 1 cut(s) 1255
BfaI CTAG 3 cut(s) 587, 1256, 1268
BfmI CTRYAG 2 cut(s) 546, 846
BfoI RGCGCY 1 cut(s) 663
BfuI GTATCC 1 cut(s) 1041
BglI GCCNNNNNGGC 2 cut(s) 243, 1107
Bme1390I CCNGG 5 cut(s) 333, 665, 740, 801, 980
Bme18I GGWCC 2 cut(s) 716, 1175
BmgT120I GGNCC 4 cut(s) 499, 716, 955, 1175
BmiI GGNNCC 4 cut(s) 661, 702, 936, 1177
BmrFI CCNGG 5 cut(s) 333, 665, 740, 801, 980
BmsI GCATC 3 cut(s) 43, 337, 1067
BoxI GACNNNNGTC 1 cut(s) 721
BpiI GAAGAC 2 cut(s) 291, 800
BpmI CTGGAG 2 cut(s) 496, 1074
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 2 cut(s) 161, 1023
BpuMI CCSGG 1 cut(s) 801
Bsa29I ATCGAT 1 cut(s) 318
BsaHI GRCGYC 3 cut(s) 174, 660, 797
BsaI GGTCTC 2 cut(s) 137, 271
BsaJI CCNNGG 6 cut(s) 84, 299, 595, 663, 719, 979
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 2 cut(s) 1152, 1182
Bsc4I CCNNNNNNNGG 4 cut(s) 183, 670, 964, 1259
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 3 cut(s) 502, 619, 1091
Bse3DI GCAATG 2 cut(s) 381, 861
BseBI CCWGG 4 cut(s) 333, 665, 740, 980
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 6 cut(s) 84, 299, 595, 663, 719, 979
BseGI GGATG 6 cut(s) 305, 328, 535, 649, 1058, 1162
BseLI CCNNNNNNNGG 4 cut(s) 183, 670, 964, 1259
BseMI GCAATG 2 cut(s) 381, 861
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 3 cut(s) 502, 619, 1091
BseRI GAGGAG 1 cut(s) 1068
BseYI CCCAGC 1 cut(s) 56
BsgI GTGCAG 1 cut(s) 1233
BshFI GGCC 4 cut(s) 237, 417, 501, 956
BshNI GGYRCC 2 cut(s) 659, 700
BshVI ATCGAT 1 cut(s) 318
BsiHKAI GWGCWC 3 cut(s) 119, 159, 231
BsiSI CCGG 3 cut(s) 281, 704, 801
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 4 cut(s) 183, 670, 964, 1259
BsmAI GTCTC 2 cut(s) 137, 271
BsmFI GGGAC 1 cut(s) 74
BsmI GAATGC 2 cut(s) 89, 765
BsnI GGCC 4 cut(s) 237, 417, 501, 956
Bso31I GGTCTC 2 cut(s) 137, 271
Bsp1286I GDGCHC 3 cut(s) 119, 159, 231
Bsp143I GATC 7 cut(s) 31, 201, 259, 315, 340, 959, 1081
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 6 cut(s) 252, 357, 372, 457, 497, 519
BspANI GGCC 4 cut(s) 237, 417, 501, 956
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 318
BspLI GGNNCC 4 cut(s) 661, 702, 936, 1177
BspMAI CTGCAG 2 cut(s) 550, 850
BspPI GGATC 1 cut(s) 1076
BspT107I GGYRCC 2 cut(s) 659, 700
BspTNI GGTCTC 2 cut(s) 137, 271
BsrDI GCAATG 2 cut(s) 381, 861
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 3 cut(s) 502, 619, 1091
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 6 cut(s) 84, 299, 595, 663, 719, 979
BssMI GATC 7 cut(s) 31, 201, 259, 315, 340, 959, 1081
BssNAI GTATAC 1 cut(s) 843
BssNI GRCGYC 3 cut(s) 174, 660, 797
BssT1I CCWWGG 2 cut(s) 84, 595
Bst1107I GTATAC 1 cut(s) 843
Bst2UI CCWGG 4 cut(s) 333, 665, 740, 980
Bst4CI ACNGT 4 cut(s) 631, 720, 871, 1240
BstACI GRCGYC 3 cut(s) 174, 660, 797
BstC8I GCNNGC 9 cut(s) 133, 419, 432, 457, 499, 607, 1099, 1108, 1278
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 3 cut(s) 84, 299, 719
BstF5I GGATG 6 cut(s) 305, 328, 535, 649, 1058, 1162
BstH2I RGCGCY 1 cut(s) 663
BstHHI GCGC 1 cut(s) 662
BstKTI GATC 7 cut(s) 34, 204, 262, 318, 343, 962, 1084
BstMAI GTCTC 2 cut(s) 137, 271
BstMBI GATC 7 cut(s) 31, 201, 259, 315, 340, 959, 1081
BstNI CCWGG 4 cut(s) 333, 665, 740, 980
BstNSI RCATGY 2 cut(s) 434, 1101
BstPAI GACNNNNGTC 1 cut(s) 721
BstSCI CCNGG 5 cut(s) 331, 663, 738, 799, 978
BstSFI CTRYAG 2 cut(s) 546, 846
BstV2I GAAGAC 2 cut(s) 291, 800
BstX2I RGATCY 1 cut(s) 1081
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 1 cut(s) 1081
BstZ17I GTATAC 1 cut(s) 843
Bsu15I ATCGAT 1 cut(s) 318
BsuI GTATCC 1 cut(s) 1041
BsuRI GGCC 4 cut(s) 237, 417, 501, 956
BsuTUI ATCGAT 1 cut(s) 318
BtgI CCRYGG 3 cut(s) 84, 299, 719
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 6 cut(s) 305, 328, 535, 649, 1058, 1162
Cac8I GCNNGC 9 cut(s) 133, 419, 432, 457, 499, 607, 1099, 1108, 1278
CaiI CAGNNNCTG 2 cut(s) 338, 1238
CfoI GCGC 1 cut(s) 662
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 4 cut(s) 499, 716, 955, 1175
ClaI ATCGAT 1 cut(s) 318
CseI GACGC 4 cut(s) 182, 436, 805, 1124
Csp6I GTAC 2 cut(s) 583, 701
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviAII CATG 8 cut(s) 85, 111, 289, 431, 464, 552, 1009, 1098
CviQI GTAC 2 cut(s) 583, 701
DdeI CTNAG 1 cut(s) 216
DinI GGCGCC 1 cut(s) 661
DpnI GATC 7 cut(s) 33, 203, 261, 317, 342, 961, 1083
DpnII GATC 7 cut(s) 31, 201, 259, 315, 340, 959, 1081
EaeI YGGCCR 2 cut(s) 235, 415
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 84, 595
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 2 cut(s) 137, 271
Eco47I GGWCC 2 cut(s) 716, 1175
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 821, 1248
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 1175
EcoRII CCWGG 4 cut(s) 331, 663, 738, 978
EcoT14I CCWWGG 2 cut(s) 84, 595
EcoT38I GRGCYC 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 661
EheI GGCGCC 1 cut(s) 661
ErhI CCWWGG 2 cut(s) 84, 595
FaeI CATG 8 cut(s) 88, 114, 292, 434, 467, 555, 1012, 1101
FaqI GGGAC 1 cut(s) 74
FatI CATG 8 cut(s) 84, 110, 288, 430, 463, 551, 1008, 1097
FauI CCCGC 2 cut(s) 464, 490
FbaI TGATCA 2 cut(s) 201, 259
FblI GTMKAC 1 cut(s) 842
FokI GGATG 6 cut(s) 292, 315, 542, 656, 1045, 1169
FriOI GRGCYC 1 cut(s) 159
FspBI CTAG 3 cut(s) 587, 1256, 1268
GlaI GCGC 1 cut(s) 661
GsaI CCCAGC 1 cut(s) 60
GsuI CTGGAG 2 cut(s) 496, 1074
HaeII RGCGCY 1 cut(s) 663
HaeIII GGCC 4 cut(s) 237, 417, 501, 956
HapII CCGG 3 cut(s) 281, 704, 801
HgaI GACGC 4 cut(s) 182, 436, 805, 1124
HhaI GCGC 1 cut(s) 662
Hin1I GRCGYC 3 cut(s) 174, 660, 797
Hin1II CATG 8 cut(s) 88, 114, 292, 434, 467, 555, 1012, 1101
Hin6I GCGC 1 cut(s) 660
HinP1I GCGC 1 cut(s) 660
HindIII AAGCTT 2 cut(s) 680, 1274
HinfI GANTC 3 cut(s) 78, 710, 1202
HpaII CCGG 3 cut(s) 281, 704, 801
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 4 cut(s) 9, 843, 874, 970
Hpy188I TCNGA 6 cut(s) 259, 340, 480, 771, 880, 1146
Hpy188III TCNNGA 6 cut(s) 35, 313, 424, 714, 1075, 1206
Hpy8I GTNNAC 4 cut(s) 9, 843, 874, 970
Hpy99I CGWCG 1 cut(s) 1118
HpyAV CCTTC 2 cut(s) 319, 766
HpyCH4III ACNGT 4 cut(s) 631, 720, 871, 1240
HpyCH4IV ACGT 3 cut(s) 225, 786, 898
HpyCH4V TGCA 8 cut(s) 548, 623, 677, 765, 848, 866, 1097, 1250
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 3 cut(s) 225, 786, 898
Hsp92I GRCGYC 3 cut(s) 174, 660, 797
Hsp92II CATG 8 cut(s) 88, 114, 292, 434, 467, 555, 1012, 1101
HspAI GCGC 1 cut(s) 660
KasI GGCGCC 1 cut(s) 659
KpnI GGTACC 1 cut(s) 704
Ksp22I TGATCA 2 cut(s) 201, 259
Kzo9I GATC 7 cut(s) 31, 201, 259, 315, 340, 959, 1081
LmnI GCTCC 4 cut(s) 114, 940, 1055, 1160
LweI GCATC 3 cut(s) 43, 337, 1067
MaeI CTAG 3 cut(s) 587, 1256, 1268
MaeII ACGT 3 cut(s) 225, 786, 898
MaeIII GTNAC 2 cut(s) 723, 776
MalI GATC 7 cut(s) 33, 203, 261, 317, 342, 961, 1083
MboI GATC 7 cut(s) 31, 201, 259, 315, 340, 959, 1081
MboII GAAGA 4 cut(s) 296, 802, 805, 827
MflI RGATCY 1 cut(s) 1081
MhlI GDGCHC 3 cut(s) 119, 159, 231
MlsI TGGCCA 2 cut(s) 237, 417
MluCI AATT 2 cut(s) 449, 533
MluNI TGGCCA 2 cut(s) 237, 417
Mly113I GGCGCC 1 cut(s) 660
MmeI TCCRAC 4 cut(s) 186, 994, 1058, 1150
MnlI CCTC 7 cut(s) 35, 225, 520, 685, 913, 1046, 1166
Mox20I TGGCCA 2 cut(s) 237, 417
MscI TGGCCA 2 cut(s) 237, 417
MseI TTAA 6 cut(s) 69, 180, 489, 828, 1125, 1242
MslI CAYNNNNRTG 1 cut(s) 468
Msp20I TGGCCA 2 cut(s) 237, 417
MspA1I CMGCKG 4 cut(s) 362, 442, 519, 851
MspI CCGG 3 cut(s) 281, 704, 801
MspR9I CCNGG 5 cut(s) 333, 665, 740, 801, 980
Mva1269I GAATGC 2 cut(s) 89, 765
MvaI CCWGG 4 cut(s) 333, 665, 740, 980
NarI GGCGCC 1 cut(s) 660
NciI CCSGG 1 cut(s) 801
NcoI CCATGG 1 cut(s) 84
NdeII GATC 7 cut(s) 31, 201, 259, 315, 340, 959, 1081
NlaIII CATG 8 cut(s) 88, 114, 292, 434, 467, 555, 1012, 1101
NlaIV GGNNCC 4 cut(s) 661, 702, 936, 1177
NmuCI GTSAC 1 cut(s) 723
NspI RCATGY 2 cut(s) 434, 1101
PaeI GCATGC 2 cut(s) 434, 1101
PctI GAATGC 2 cut(s) 89, 765
PfeI GAWTC 3 cut(s) 78, 710, 1202
PflMI CCANNNNNTGG 3 cut(s) 183, 670, 1259
PluTI GGCGCC 1 cut(s) 663
PpuMI RGGWCCY 1 cut(s) 1175
PshAI GACNNNNGTC 1 cut(s) 721
PshBI ATTAAT 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 1175
Psp6I CCWGG 4 cut(s) 331, 663, 738, 978
PspFI CCCAGC 1 cut(s) 56
PspGI CCWGG 4 cut(s) 331, 663, 738, 978
PspN4I GGNNCC 4 cut(s) 661, 702, 936, 1177
PspPI GGNCC 4 cut(s) 499, 716, 955, 1175
PspPPI RGGWCCY 1 cut(s) 1175
PstI CTGCAG 2 cut(s) 550, 850
PstNI CAGNNNCTG 2 cut(s) 338, 1238
PsuI RGATCY 1 cut(s) 1081
PvuII CAGCTG 3 cut(s) 362, 442, 851
RsaI GTAC 2 cut(s) 584, 702
RsaNI GTAC 2 cut(s) 583, 701
RseI CAYNNNNRTG 1 cut(s) 468
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 6 cut(s) 69, 180, 489, 828, 1125, 1242
Sau3AI GATC 7 cut(s) 31, 201, 259, 315, 340, 959, 1081
Sau96I GGNCC 4 cut(s) 499, 716, 955, 1175
ScrFI CCNGG 5 cut(s) 333, 665, 740, 801, 980
SduI GDGCHC 3 cut(s) 119, 159, 231
SfaNI GCATC 3 cut(s) 43, 337, 1067
SfcI CTRYAG 2 cut(s) 546, 846
SfoI GGCGCC 1 cut(s) 661
SinI GGWCC 2 cut(s) 716, 1175
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 2 cut(s) 140, 1038
SmoI CTYRAG 2 cut(s) 140, 1038
SpeI ACTAGT 1 cut(s) 1255
SphI GCATGC 2 cut(s) 434, 1101
Sse9I AATT 2 cut(s) 449, 533
SsiI CCGC 6 cut(s) 252, 357, 372, 457, 497, 519
SspDI GGCGCC 1 cut(s) 659
SspMI CTAG 3 cut(s) 587, 1256, 1268
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 5 cut(s) 331, 663, 738, 799, 978
StyI CCWWGG 2 cut(s) 84, 595
TaaI ACNGT 4 cut(s) 631, 720, 871, 1240
TaiI ACGT 3 cut(s) 228, 789, 901
TaqI TCGA 3 cut(s) 318, 1113, 1205
TasI AATT 2 cut(s) 449, 533
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 3 cut(s) 78, 710, 1202
Tru1I TTAA 6 cut(s) 69, 180, 489, 828, 1125, 1242
Tru9I TTAA 6 cut(s) 69, 180, 489, 828, 1125, 1242
TseFI GTSAC 1 cut(s) 723
Tsp45I GTSAC 1 cut(s) 723
TspDTI ATGAA 6 cut(s) 17, 99, 546, 548, 742, 1034
TspGWI ACGGA 2 cut(s) 316, 1214
Van91I CCANNNNNTGG 3 cut(s) 183, 670, 1259
VpaK11BI GGWCC 2 cut(s) 716, 1175
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 1 cut(s) 449
XceI RCATGY 2 cut(s) 434, 1101
XcmI CCANNNNNNNNNTGG 2 cut(s) 245, 1219
XmiI GTMKAC 1 cut(s) 842
XspI CTAG 3 cut(s) 587, 1256, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.