Rh4CG461200

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003358
Physical Location & Seq
Reverse (-)
69250958 .. 69252691
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG461200.1

Sequence Viewer

Length: 1149 bp
ATGCAGATTAATTTGGTGGGAGGCTACTACGATGCCGGAGACAATCTCAAGTTGGGATTTCCAATGGCCTTCACAGTAACAATGCTGTCATGGAGCACGATCGAATTCCAAAGCAAACTCAAGGAGTCAAGGCCAAATATGCATGAGATCGCTAATGCTCTTCGTGCTATCAAGTGGGGAACAGATTACTTGATGAAAGCACATCCACAGCCCGAAGAGCTTTATGGGCAAATTGGGGGTGTTGATTCAGACCATCAATGTTGGCAGAGACCAGAGGACATGACCACCCCGAGGACCGTGTTCATGATTGATGACCAACACCCGGGATCTGATCTCGCCGCTGAAACTGCTGCTGCTTTGGCTGCTGCCTCAATTTCTTTAGAACGTTTTGATAGAAGTTACTCATCCCAACTCGTAACTCATGCAAAGCAGCTATTTGATTTTGCCCGCAATCATCCTGGTAATTATCAAAACAGCATCCCTGAGGCAGGAAAAGTCTACGCCAGTAGTGGGTATCAGGACGAGATGCTGTGGGCAGCGGCATGGCTCCATCGTGCTACTAATGAACAGAAGTACCTCAACTTCCTAAGCGAAGCCGGAGATATAGGTGGAACAAGAACAACATTCTCATGGGATGACAAATTCGTTGGCGCACAGGTTTTGGCTGCAATGAAAGGAAACGACAACGTGAAGAAGACACCCGGAGGCTTGCTTTGGTTTCTGCCATGGAATAACAATCAATACGTTGCCACTGCTACATTTGTCATCACTGTTTATTCTATTTATTTGGACGCAAAAAAGGCCTCCATCAAGTGTCCTGGTGGCGCTGCTCACCCCTCTGATCTCATTGCATCTGCCAAGTCACAGGTGGATTACATCTTGGGATTAAACCCGAAAAACATGAGCTACATGGTTGGATATGGATCGAATTATCCGAAGCAAATTCATCACAGAGGAGCATCAATCGTCTCAATCAAGAAGGATCCAGCTCCTGTGACATGCCAAGGCGGGTTTGACTGGTTTCACAAACCCGAGCCCAACCCGAACGTGCTGGAGGGAGCAATTGTGACCCCAGATGAAAATGATGGTTTTGCGGACGACAGGAACAACTTTCAGTTAGCTGAACGACCGTTACTAATGCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

382

Amino Acids

42.39

Weight (kDa)

5.83

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 3 - 375 3.2e-120 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 498
AciI CCGC 5 cut(s) 339, 448, 539, 1008, 1094
AclI AACGTT 1 cut(s) 385
AclWI GGATC 4 cut(s) 334, 931, 977, 990
AcsI RAATTY 3 cut(s) 104, 641, 942
AfaI GTAC 1 cut(s) 575
AfiI CCNNNNNNNGG 4 cut(s) 291, 322, 488, 510
AjnI CCWGG 2 cut(s) 457, 817
AluBI AGCT 5 cut(s) 220, 433, 906, 989, 1121
AluI AGCT 5 cut(s) 220, 433, 906, 989, 1121
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 3 cut(s) 33, 262, 973
AlwI GGATC 4 cut(s) 334, 931, 977, 990
AlwNI CAGNNNCTG 1 cut(s) 992
Ama87I CYCGRG 3 cut(s) 289, 322, 1031
AoxI GGCC 3 cut(s) 66, 131, 801
ApeKI GCWGC 8 cut(s) 350, 353, 362, 365, 430, 536, 665, 827
ApoI RAATTY 3 cut(s) 104, 641, 942
AseI ATTAAT 1 cut(s) 9
AspLEI GCGC 2 cut(s) 653, 827
AspS9I GGNCC 1 cut(s) 294
AsuC2I CCSGG 3 cut(s) 323, 324, 702
AsuHPI GGTGA 1 cut(s) 824
AvaI CYCGRG 3 cut(s) 289, 322, 1031
AvaII GGWCC 1 cut(s) 294
AxyI CCTNAGG 1 cut(s) 483
BaeI ACNNNNGTAYC 2 cut(s) 557, 590
BamHI GGATCC 1 cut(s) 982
BanII GRGCYC 1 cut(s) 1038
BarI GAAGNNNNNNTAC 2 cut(s) 566, 598
BbsI GAAGAC 1 cut(s) 701
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 8 cut(s) 337, 340, 349, 352, 442, 548, 652, 814
BccI CCATC 4 cut(s) 261, 558, 815, 1079
BciT130I CCWGG 2 cut(s) 459, 819
BcnI CCSGG 3 cut(s) 323, 324, 702
BcoDI GTCTC 3 cut(s) 33, 262, 973
BfaI CTAG 1 cut(s) 1147
BfoI RGCGCY 1 cut(s) 828
Bme1390I CCNGG 5 cut(s) 323, 324, 459, 702, 819
Bme18I GGWCC 1 cut(s) 294
BmeT110I CYCGRG 3 cut(s) 289, 322, 1031
BmgT120I GGNCC 1 cut(s) 294
BmiI GGNNCC 2 cut(s) 548, 984
BmrFI CCNGG 5 cut(s) 323, 324, 459, 702, 819
BmsI GCATC 5 cut(s) 22, 486, 516, 860, 968
BpiI GAAGAC 1 cut(s) 701
BplI GAGNNNNNCTC 2 cut(s) 30, 62
BpmI CTGGAG 1 cut(s) 1073
Bpu10I CCTNAGC 1 cut(s) 587
BpuEI CTTGAG 2 cut(s) 32, 104
BpuMI CCSGG 3 cut(s) 323, 324, 702
BsaBI GATNNNNATC 1 cut(s) 922
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 4 cut(s) 290, 322, 725, 1003
BsaXI ACNNNNNCTCC 3 cut(s) 1050, 1080, 1125
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 322, 488, 510
Bse1I ACTGG 2 cut(s) 504, 1022
Bse21I CCTNAGG 1 cut(s) 483
Bse3DI GCAATG 2 cut(s) 675, 846
Bse8I GATNNNNATC 1 cut(s) 922
BseBI CCWGG 2 cut(s) 459, 819
BseDI CCNNGG 4 cut(s) 290, 322, 725, 1003
BseGI GGATG 5 cut(s) 202, 404, 454, 477, 640
BseJI GATNNNNATC 1 cut(s) 922
BseLI CCNNNNNNNGG 4 cut(s) 291, 322, 488, 510
BseMI GCAATG 2 cut(s) 675, 846
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 2 cut(s) 504, 1022
BseRI GAGGAG 1 cut(s) 969
BseXI GCAGC 8 cut(s) 337, 340, 349, 352, 442, 548, 652, 814
Bsh1285I CGRYCG 2 cut(s) 102, 1130
BshFI GGCC 3 cut(s) 68, 133, 803
BsiEI CGRYCG 2 cut(s) 102, 1130
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 3 cut(s) 289, 322, 1031
BsiSI CCGG 4 cut(s) 36, 323, 597, 702
BslI CCNNNNNNNGG 4 cut(s) 291, 322, 488, 510
BsmAI GTCTC 3 cut(s) 33, 262, 973
BsmBI CGTCTC 1 cut(s) 973
BsnI GGCC 3 cut(s) 68, 133, 803
Bso31I GGTCTC 1 cut(s) 262
BsoBI CYCGRG 3 cut(s) 289, 322, 1031
Bsp1286I GDGCHC 2 cut(s) 98, 1038
Bsp143I GATC 7 cut(s) 99, 147, 326, 331, 841, 923, 982
Bsp19I CCATGG 1 cut(s) 725
BspACI CCGC 5 cut(s) 339, 448, 539, 1008, 1094
BspANI GGCC 3 cut(s) 68, 133, 803
BspCNI CTCAG 1 cut(s) 475
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 2 cut(s) 548, 984
BspPI GGATC 4 cut(s) 334, 931, 977, 990
BspQI GCTCTTC 2 cut(s) 165, 210
BspTNI GGTCTC 1 cut(s) 262
BsrDI GCAATG 2 cut(s) 675, 846
BsrI ACTGG 2 cut(s) 504, 1022
BssECI CCNNGG 4 cut(s) 290, 322, 725, 1003
BssMI GATC 7 cut(s) 99, 147, 326, 331, 841, 923, 982
BssT1I CCWWGG 2 cut(s) 725, 1003
Bst2UI CCWGG 2 cut(s) 459, 819
Bst4CI ACNGT 4 cut(s) 76, 298, 772, 1131
Bst6I CTCTTC 2 cut(s) 165, 210
BstC8I GCNNGC 2 cut(s) 448, 710
BstDEI CTNAG 2 cut(s) 483, 587
BstDSI CCRYGG 1 cut(s) 725
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 5 cut(s) 202, 404, 454, 477, 640
BstH2I RGCGCY 1 cut(s) 828
BstHHI GCGC 2 cut(s) 653, 827
BstKTI GATC 7 cut(s) 102, 150, 329, 334, 844, 926, 985
BstMAI GTCTC 3 cut(s) 33, 262, 973
BstMBI GATC 7 cut(s) 99, 147, 326, 331, 841, 923, 982
BstMCI CGRYCG 2 cut(s) 102, 1130
BstMWI GCNNNNNNNGC 8 cut(s) 139, 164, 217, 226, 347, 359, 362, 800
BstNI CCWGG 2 cut(s) 459, 819
BstNSI RCATGY 1 cut(s) 1002
BstSCI CCNGG 5 cut(s) 321, 322, 457, 700, 817
BstV1I GCAGC 8 cut(s) 337, 340, 349, 352, 442, 548, 652, 814
BstV2I GAAGAC 1 cut(s) 701
BstX2I RGATCY 2 cut(s) 326, 982
BstYI RGATCY 2 cut(s) 326, 982
Bsu36I CCTNAGG 1 cut(s) 483
BsuRI GGCC 3 cut(s) 68, 133, 803
BtgI CCRYGG 1 cut(s) 725
BtsCI GGATG 5 cut(s) 202, 404, 454, 477, 640
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 2 cut(s) 750, 768
Cac8I GCNNGC 2 cut(s) 448, 710
CaiI CAGNNNCTG 1 cut(s) 992
CciI TCATGA 1 cut(s) 303
CfoI GCGC 2 cut(s) 653, 827
Cfr13I GGNCC 1 cut(s) 294
Cfr9I CCCGGG 1 cut(s) 322
CseI GACGC 1 cut(s) 800
Csp6I GTAC 1 cut(s) 574
CviQI GTAC 1 cut(s) 574
DdeI CTNAG 2 cut(s) 483, 587
DpnI GATC 7 cut(s) 101, 149, 328, 333, 843, 925, 984
DpnII GATC 7 cut(s) 99, 147, 326, 331, 841, 923, 982
Eam1104I CTCTTC 2 cut(s) 165, 210
EarI CTCTTC 2 cut(s) 165, 210
Eco130I CCWWGG 2 cut(s) 725, 1003
Eco147I AGGCCT 1 cut(s) 803
Eco24I GRGCYC 1 cut(s) 1038
Eco31I GGTCTC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 294
Eco81I CCTNAGG 1 cut(s) 483
Eco88I CYCGRG 3 cut(s) 289, 322, 1031
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 457, 817
EcoT14I CCWWGG 2 cut(s) 725, 1003
EcoT22I ATGCAT 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 1038
ErhI CCWWGG 2 cut(s) 725, 1003
Esp3I CGTCTC 1 cut(s) 973
FauI CCCGC 2 cut(s) 455, 1001
FblI GTMKAC 1 cut(s) 498
FokI GGATG 5 cut(s) 189, 391, 441, 464, 647
FriOI GRGCYC 1 cut(s) 1038
FspBI CTAG 1 cut(s) 1147
GlaI GCGC 2 cut(s) 652, 826
GsuI CTGGAG 1 cut(s) 1073
HaeII RGCGCY 1 cut(s) 828
HaeIII GGCC 3 cut(s) 68, 133, 803
HapII CCGG 4 cut(s) 36, 323, 597, 702
HgaI GACGC 1 cut(s) 800
HhaI GCGC 2 cut(s) 653, 827
Hin6I GCGC 2 cut(s) 651, 825
HinP1I GCGC 2 cut(s) 651, 825
HinfI GANTC 2 cut(s) 125, 245
HpaII CCGG 4 cut(s) 36, 323, 597, 702
HphI GGTGA 1 cut(s) 824
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 4 cut(s) 250, 331, 841, 936
Hpy188III TCNNGA 3 cut(s) 304, 518, 976
Hpy8I GTNNAC 1 cut(s) 499
HpyAV CCTTC 2 cut(s) 79, 973
HpyCH4III ACNGT 4 cut(s) 76, 298, 772, 1131
HpyCH4IV ACGT 4 cut(s) 385, 687, 744, 1047
HpyCH4V TGCA 5 cut(s) 4, 142, 425, 668, 851
HpyF10VI GCNNNNNNNGC 8 cut(s) 139, 164, 217, 226, 347, 359, 362, 800
HpyF3I CTNAG 2 cut(s) 483, 587
HpySE526I ACGT 4 cut(s) 385, 687, 744, 1047
HspAI GCGC 2 cut(s) 651, 825
Kzo9I GATC 7 cut(s) 99, 147, 326, 331, 841, 923, 982
LguI GCTCTTC 2 cut(s) 165, 210
LmnI GCTCC 6 cut(s) 93, 552, 956, 994, 1058, 1146
Lsp1109I GCAGC 8 cut(s) 337, 340, 349, 352, 442, 548, 652, 814
LweI GCATC 5 cut(s) 22, 486, 516, 860, 968
MaeI CTAG 1 cut(s) 1147
MaeII ACGT 4 cut(s) 385, 687, 744, 1047
MaeIII GTNAC 7 cut(s) 76, 398, 415, 861, 994, 1066, 1131
MalI GATC 7 cut(s) 101, 149, 328, 333, 843, 925, 984
MboI GATC 7 cut(s) 99, 147, 326, 331, 841, 923, 982
MboII GAAGA 4 cut(s) 152, 227, 703, 706
MfeI CAATTG 1 cut(s) 1062
MflI RGATCY 2 cut(s) 326, 982
MhlI GDGCHC 2 cut(s) 98, 1038
MluCI AATT 9 cut(s) 10, 104, 231, 372, 463, 641, 928, 942, 1062
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 895
Mph1103I ATGCAT 1 cut(s) 144
MseI TTAA 2 cut(s) 9, 887
MslI CAYNNNNRTG 1 cut(s) 628
MspA1I CMGCKG 2 cut(s) 341, 539
MspI CCGG 4 cut(s) 36, 323, 597, 702
MspR9I CCNGG 5 cut(s) 323, 324, 459, 702, 819
MunI CAATTG 1 cut(s) 1062
MvaI CCWGG 2 cut(s) 459, 819
MwoI GCNNNNNNNGC 8 cut(s) 139, 164, 217, 226, 347, 359, 362, 800
NciI CCSGG 3 cut(s) 323, 324, 702
NcoI CCATGG 1 cut(s) 725
NdeII GATC 7 cut(s) 99, 147, 326, 331, 841, 923, 982
NlaIV GGNNCC 2 cut(s) 548, 984
NmuCI GTSAC 3 cut(s) 861, 994, 1066
NsiI ATGCAT 1 cut(s) 144
NspI RCATGY 1 cut(s) 1002
PagI TCATGA 1 cut(s) 303
PceI AGGCCT 1 cut(s) 803
PciSI GCTCTTC 2 cut(s) 165, 210
PcsI WCGNNNNNNNCGW 1 cut(s) 932
PfeI GAWTC 1 cut(s) 245
Ple19I CGATCG 1 cut(s) 102
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PshBI ATTAAT 1 cut(s) 9
Psp1406I AACGTT 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 457, 817
PspGI CCWGG 2 cut(s) 457, 817
PspN4I GGNNCC 2 cut(s) 548, 984
PspPI GGNCC 1 cut(s) 294
PsrI GAACNNNNNNTAC 2 cut(s) 1116, 1148
PstNI CAGNNNCTG 1 cut(s) 992
PsuI RGATCY 2 cut(s) 326, 982
PvuI CGATCG 1 cut(s) 102
RsaI GTAC 1 cut(s) 575
RsaNI GTAC 1 cut(s) 574
RseI CAYNNNNRTG 1 cut(s) 628
SapI GCTCTTC 2 cut(s) 165, 210
SaqAI TTAA 2 cut(s) 9, 887
Sau3AI GATC 7 cut(s) 99, 147, 326, 331, 841, 923, 982
Sau96I GGNCC 1 cut(s) 294
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 5 cut(s) 323, 324, 459, 702, 819
SduI GDGCHC 2 cut(s) 98, 1038
SfaNI GCATC 5 cut(s) 22, 486, 516, 860, 968
SinI GGWCC 1 cut(s) 294
SmaI CCCGGG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 628
SmlI CTYRAG 2 cut(s) 47, 119
SmoI CTYRAG 2 cut(s) 47, 119
Sse9I AATT 9 cut(s) 10, 104, 231, 372, 463, 641, 928, 942, 1062
SseBI AGGCCT 1 cut(s) 803
SsiI CCGC 5 cut(s) 339, 448, 539, 1008, 1094
SspMI CTAG 1 cut(s) 1147
StuI AGGCCT 1 cut(s) 803
StyD4I CCNGG 5 cut(s) 321, 322, 457, 700, 817
StyI CCWWGG 2 cut(s) 725, 1003
TaaI ACNGT 4 cut(s) 76, 298, 772, 1131
TaiI ACGT 4 cut(s) 388, 690, 747, 1050
TaqI TCGA 2 cut(s) 102, 926
TasI AATT 9 cut(s) 10, 104, 231, 372, 463, 641, 928, 942, 1062
TauI GCSGC 2 cut(s) 341, 542
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 9, 887
Tru9I TTAA 2 cut(s) 9, 887
TscAI CASTG 2 cut(s) 757, 775
TseFI GTSAC 3 cut(s) 861, 994, 1066
TseI GCWGC 8 cut(s) 350, 353, 362, 365, 430, 536, 665, 827
Tsp45I GTSAC 3 cut(s) 861, 994, 1066
TspDTI ATGAA 6 cut(s) 209, 292, 579, 686, 935, 1092
TspMI CCCGGG 1 cut(s) 322
TspRI CASTG 2 cut(s) 757, 775
VpaK11BI GGWCC 1 cut(s) 294
VspI ATTAAT 1 cut(s) 9
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 3 cut(s) 104, 641, 942
XceI RCATGY 1 cut(s) 1002
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XmaI CCCGGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 498
XspI CTAG 1 cut(s) 1147
Zsp2I ATGCAT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.