Rw4G037180

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003358
Physical Location & Seq
Reverse (-)
63902501 .. 63904609
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037180.1

Sequence Viewer

Length: 1479 bp
ATGATGATGTCTGTGCAGAGCAGCAGACGGGTCTTTGGTTTGGTTATTGGACCATTATCTATGCTTTTATTGTTCCAAGGAATTGCTTGTGAAGCAGACTATACTCAAGCACTTGCCAAGTCATTGTTGTATTATGAGGGTCAACGCTCAGGGAAGTTGCCCCCTATTGGCAAACTCCAGAGAGTGGCGTGGCGAGGAGATTCCGGACTAGAAGACGGCAGTGATGCTGGAATTGATTTGGTGGGAGGCTACTACGATGCCGGAGACAACCTCAAGTTGGGATTTCCAATGGCCTTCTCAGTAACAATGCTGTCATGGAGCACGATCGAATTCCAAAGCAAGCTCAAGGCGAAATATGAGATCGCCAATGCTCTTCGGGCTATCAAGTGGGGAACAGATTACTTGATAAAAGCACATCCACAGCCCGAAGTGCTTTATGGGCATATTGGGGGTGTTGATTCAGACCATCAATGTTGGCAGAGACCAGAGGACATGACCACCCCGAGGACCGTGTTCATGATTGATGACCAACACCCGGGATCTGATCTCGCCGCTGAAACTGCTGCTGCTTTGGCTGCTGCCTCAATTGCTTTTGAACGATTTGATAGAAGTTACTCATCCCAACTCGTAACTCATGCAAAGCAGCTATTTGATTTTGCACGCAATCATCCTGGTACGTATCAAAACAGCATCCCTGAGGCAGGAAAAGTCTACGCCAGTAGTGGGTATCAGGACGAGATGCTGTGGGCAGCGGCATGGCTCCATCGTGCTACTAATGAACAGAAGTACCTCAACTTCCTAAGCGAAGCGGGAGATATAGGTGGAACAAGAACAACATTCTCATGGGATGACAAATTCGTCGGCGCACAGGTTTTGGCAGCAATGCTTGTAATGAAGGGAAAGGTTGATTCTTCAGGAAAATGGGGGGAGTATAAGCGCCATGCAGAGGAATTCATATGTTCATGCGTTCAGAAAGGAAACAACAACGTGAAGAAGACACCCGGAGGCTTGCTTTGGTTTCTACCATGGAATAACAATCAATACGTTGCCACTGCTACATTTGTCACCACTGTTTATTCCGTTTATTTGGACGCGAAAAAAGCCTCCATCAACTGTCCTGGTGGCGCTGCTCACCCCTTTGATCTCATTGCATCTGCCAAGTCACAGATGGATTACATCTTGGGCTCAAACCCGAAAAACATGAGCTATATGGTTGGATATGGATCGAATTATCCGAAGCAAATTCATCACAGAGGAGCATCAATCGTCTCAATCAAGAAGGATCCAGCTCCTGTGACATGCCAAGGTGGGTTTGACTGGTTTCACAGACCCGAGCCCAACCCGAACGTGCTGGAGGGAGCAATTGTGACCCCAGACGAAAATGATGGTTTTGCGGACGACAGGAACAACTTTCAGTTAGCTGAACCAACGACCGTTACTAATGCTCCACTAGTTGGTGTCTTAGCTCACCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

492

Amino Acids

54.06

Weight (kDa)

6.12

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 34 - 488 3.4e-156 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
AccB7I CCANNNNNTGG 2 cut(s) 184, 1454
AccI GTMKAC 1 cut(s) 711
AccII CGCG 1 cut(s) 1094
AccIII TCCGGA 1 cut(s) 203
AciI CCGC 4 cut(s) 552, 752, 809, 1394
AclWI GGATC 4 cut(s) 547, 1231, 1277, 1290
AcsI RAATTY 4 cut(s) 329, 854, 950, 1242
AcuI CTGAAG 1 cut(s) 897
AfaI GTAC 2 cut(s) 676, 788
AfiI CCNNNNNNNGG 9 cut(s) 167, 184, 277, 504, 535, 701, 723, 946, 1454
AgsI TTSAA 1 cut(s) 596
AhlI ACTAGT 1 cut(s) 1450
AjnI CCWGG 2 cut(s) 670, 1117
AluBI AGCT 6 cut(s) 343, 646, 1206, 1289, 1421, 1466
AluI AGCT 6 cut(s) 343, 646, 1206, 1289, 1421, 1466
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 3 cut(s) 258, 475, 1273
AlwI GGATC 4 cut(s) 547, 1231, 1277, 1290
AlwNI CAGNNNCTG 1 cut(s) 1292
Ama87I CYCGRG 3 cut(s) 502, 535, 1331
Aor13HI TCCGGA 1 cut(s) 203
AoxI GGCC 1 cut(s) 291
ApeKI GCWGC 9 cut(s) 21, 563, 566, 575, 578, 643, 749, 878, 1127
ApoI RAATTY 4 cut(s) 329, 854, 950, 1242
AspLEI GCGC 3 cut(s) 866, 939, 1127
AspS9I GGNCC 2 cut(s) 50, 507
AsuC2I CCSGG 3 cut(s) 536, 537, 1002
AsuHPI GGTGA 3 cut(s) 1057, 1124, 1460
AvaI CYCGRG 3 cut(s) 502, 535, 1331
AvaII GGWCC 2 cut(s) 50, 507
AxyI CCTNAGG 1 cut(s) 696
BaeI ACNNNNGTAYC 2 cut(s) 770, 803
BamHI GGATCC 1 cut(s) 1282
BanII GRGCYC 2 cut(s) 1187, 1338
BarI GAAGNNNNNNTAC 2 cut(s) 779, 811
BbsI GAAGAC 2 cut(s) 219, 1001
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 9 cut(s) 33, 550, 553, 562, 565, 655, 761, 890, 1114
BccI CCATC 5 cut(s) 474, 771, 1115, 1162, 1379
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 672, 1119
BcnI CCSGG 3 cut(s) 536, 537, 1002
BcoDI GTCTC 3 cut(s) 258, 475, 1273
BcuI ACTAGT 1 cut(s) 1450
BfaI CTAG 2 cut(s) 209, 1451
BfoI RGCGCY 2 cut(s) 940, 1128
Bme1390I CCNGG 5 cut(s) 536, 537, 672, 1002, 1119
Bme18I GGWCC 2 cut(s) 50, 507
BmeT110I CYCGRG 3 cut(s) 502, 535, 1331
BmgT120I GGNCC 2 cut(s) 50, 507
BmiI GGNNCC 2 cut(s) 761, 1284
BmrFI CCNGG 5 cut(s) 536, 537, 672, 1002, 1119
BmsI GCATC 6 cut(s) 214, 247, 699, 729, 1160, 1268
BpiI GAAGAC 2 cut(s) 219, 1001
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 2 cut(s) 161, 1373
Bpu10I CCTNAGC 2 cut(s) 148, 800
BpuEI CTTGAG 3 cut(s) 90, 257, 329
BpuMI CCSGG 3 cut(s) 536, 537, 1002
BsaAI YACGTR 1 cut(s) 678
BsaBI GATNNNNATC 1 cut(s) 1222
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 5 cut(s) 76, 503, 535, 1025, 1303
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 4 cut(s) 1350, 1380, 1429, 1459
Bsc4I CCNNNNNNNGG 9 cut(s) 167, 184, 277, 504, 535, 701, 723, 946, 1454
Bse1I ACTGG 2 cut(s) 717, 1322
Bse21I CCTNAGG 1 cut(s) 696
Bse3DI GCAATG 2 cut(s) 888, 1146
Bse8I GATNNNNATC 1 cut(s) 1222
BseAI TCCGGA 1 cut(s) 203
BseBI CCWGG 2 cut(s) 672, 1119
BseDI CCNNGG 5 cut(s) 76, 503, 535, 1025, 1303
BseGI GGATG 5 cut(s) 415, 617, 667, 690, 853
BseJI GATNNNNATC 1 cut(s) 1222
BseLI CCNNNNNNNGG 9 cut(s) 167, 184, 277, 504, 535, 701, 723, 946, 1454
BseMI GCAATG 2 cut(s) 888, 1146
BseMII CTCAG 3 cut(s) 162, 312, 687
BseNI ACTGG 2 cut(s) 717, 1322
BseRI GAGGAG 2 cut(s) 210, 1269
BseXI GCAGC 9 cut(s) 33, 550, 553, 562, 565, 655, 761, 890, 1114
BsgI GTGCAG 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 1094
Bsh1285I CGRYCG 2 cut(s) 327, 1434
BshFI GGCC 1 cut(s) 293
BsiEI CGRYCG 2 cut(s) 327, 1434
BsiHKAI GWGCWC 1 cut(s) 323
BsiHKCI CYCGRG 3 cut(s) 502, 535, 1331
BsiSI CCGG 4 cut(s) 204, 261, 536, 1002
BslI CCNNNNNNNGG 9 cut(s) 167, 184, 277, 504, 535, 701, 723, 946, 1454
BsmAI GTCTC 3 cut(s) 258, 475, 1273
BsmBI CGTCTC 1 cut(s) 1273
BsnI GGCC 1 cut(s) 293
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 3 cut(s) 502, 535, 1331
Bsp1286I GDGCHC 3 cut(s) 323, 1187, 1338
Bsp13I TCCGGA 1 cut(s) 203
Bsp143I GATC 7 cut(s) 324, 360, 539, 544, 1141, 1223, 1282
Bsp19I CCATGG 1 cut(s) 1025
BspACI CCGC 4 cut(s) 552, 752, 809, 1394
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 3 cut(s) 161, 311, 688
BspEI TCCGGA 1 cut(s) 203
BspFNI CGCG 1 cut(s) 1094
BspHI TCATGA 1 cut(s) 516
BspLI GGNNCC 2 cut(s) 761, 1284
BspPI GGATC 4 cut(s) 547, 1231, 1277, 1290
BspQI GCTCTTC 1 cut(s) 378
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 2 cut(s) 888, 1146
BsrI ACTGG 2 cut(s) 717, 1322
BssECI CCNNGG 5 cut(s) 76, 503, 535, 1025, 1303
BssMI GATC 7 cut(s) 324, 360, 539, 544, 1141, 1223, 1282
BssT1I CCWWGG 3 cut(s) 76, 1025, 1303
Bst2UI CCWGG 2 cut(s) 672, 1119
Bst4CI ACNGT 4 cut(s) 511, 1072, 1115, 1435
Bst6I CTCTTC 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 678
BstC8I GCNNGC 3 cut(s) 341, 661, 1010
BstDEI CTNAG 5 cut(s) 148, 298, 696, 800, 1462
BstDSI CCRYGG 1 cut(s) 1025
BstENI CCTNNNNNAGG 1 cut(s) 699
BstF5I GGATG 5 cut(s) 415, 617, 667, 690, 853
BstFNI CGCG 1 cut(s) 1094
BstH2I RGCGCY 2 cut(s) 940, 1128
BstHHI GCGC 3 cut(s) 866, 939, 1127
BstKTI GATC 7 cut(s) 327, 363, 542, 547, 1144, 1226, 1285
BstMAI GTCTC 3 cut(s) 258, 475, 1273
BstMBI GATC 7 cut(s) 324, 360, 539, 544, 1141, 1223, 1282
BstMCI CGRYCG 2 cut(s) 327, 1434
BstNI CCWGG 2 cut(s) 672, 1119
BstNSI RCATGY 1 cut(s) 1302
BstSCI CCNGG 5 cut(s) 534, 535, 670, 1000, 1117
BstSNI TACGTA 1 cut(s) 678
BstUI CGCG 1 cut(s) 1094
BstV1I GCAGC 9 cut(s) 33, 550, 553, 562, 565, 655, 761, 890, 1114
BstV2I GAAGAC 2 cut(s) 219, 1001
BstX2I RGATCY 2 cut(s) 539, 1282
BstYI RGATCY 2 cut(s) 539, 1282
Bsu36I CCTNAGG 1 cut(s) 696
BsuRI GGCC 1 cut(s) 293
BtgI CCRYGG 1 cut(s) 1025
BtsCI GGATG 5 cut(s) 415, 617, 667, 690, 853
BtsI GCAGTG 2 cut(s) 226, 1050
BtsIMutI CAGTG 3 cut(s) 226, 1050, 1068
Cac8I GCNNGC 3 cut(s) 341, 661, 1010
CaiI CAGNNNCTG 1 cut(s) 1292
CciI TCATGA 1 cut(s) 516
CfoI GCGC 3 cut(s) 866, 939, 1127
Cfr13I GGNCC 2 cut(s) 50, 507
Cfr9I CCCGGG 1 cut(s) 535
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 2 cut(s) 675, 787
CviQI GTAC 2 cut(s) 675, 787
DdeI CTNAG 5 cut(s) 148, 298, 696, 800, 1462
DpnI GATC 7 cut(s) 326, 362, 541, 546, 1143, 1225, 1284
DpnII GATC 7 cut(s) 324, 360, 539, 544, 1141, 1223, 1282
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco105I TACGTA 1 cut(s) 678
Eco130I CCWWGG 3 cut(s) 76, 1025, 1303
Eco24I GRGCYC 2 cut(s) 1187, 1338
Eco31I GGTCTC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 50, 507
Eco57I CTGAAG 1 cut(s) 897
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 3 cut(s) 502, 535, 1331
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoRI GAATTC 2 cut(s) 329, 950
EcoRII CCWGG 2 cut(s) 670, 1117
EcoT14I CCWWGG 3 cut(s) 76, 1025, 1303
EcoT38I GRGCYC 2 cut(s) 1187, 1338
ErhI CCWWGG 3 cut(s) 76, 1025, 1303
Esp3I CGTCTC 1 cut(s) 1273
FauI CCCGC 1 cut(s) 802
FauNDI CATATG 1 cut(s) 956
FblI GTMKAC 1 cut(s) 711
FokI GGATG 5 cut(s) 402, 604, 654, 677, 860
FriOI GRGCYC 2 cut(s) 1187, 1338
FspBI CTAG 2 cut(s) 209, 1451
GlaI GCGC 3 cut(s) 865, 938, 1126
GsuI CTGGAG 2 cut(s) 161, 1373
HaeII RGCGCY 2 cut(s) 940, 1128
HaeIII GGCC 1 cut(s) 293
HapII CCGG 4 cut(s) 204, 261, 536, 1002
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 3 cut(s) 866, 939, 1127
Hin6I GCGC 3 cut(s) 864, 937, 1125
HinP1I GCGC 3 cut(s) 864, 937, 1125
HincII GTYRAC 1 cut(s) 143
HindII GTYRAC 1 cut(s) 143
HinfI GANTC 3 cut(s) 200, 458, 908
HpaII CCGG 4 cut(s) 204, 261, 536, 1002
HphI GGTGA 3 cut(s) 1057, 1124, 1460
Hpy166II GTNNAC 2 cut(s) 143, 712
Hpy188I TCNGA 4 cut(s) 463, 544, 972, 1236
Hpy188III TCNNGA 6 cut(s) 178, 204, 517, 731, 915, 1276
Hpy8I GTNNAC 2 cut(s) 143, 712
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 3 cut(s) 304, 889, 1273
HpyCH4III ACNGT 4 cut(s) 511, 1072, 1115, 1435
HpyCH4IV ACGT 4 cut(s) 677, 987, 1044, 1347
HpyCH4V TGCA 5 cut(s) 16, 638, 659, 944, 1151
HpyF3I CTNAG 5 cut(s) 148, 298, 696, 800, 1462
HpySE526I ACGT 4 cut(s) 677, 987, 1044, 1347
HspAI GCGC 3 cut(s) 864, 937, 1125
Kpn2I TCCGGA 1 cut(s) 203
Kzo9I GATC 7 cut(s) 324, 360, 539, 544, 1141, 1223, 1282
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 6 cut(s) 318, 765, 1256, 1294, 1358, 1450
Lsp1109I GCAGC 9 cut(s) 33, 550, 553, 562, 565, 655, 761, 890, 1114
LweI GCATC 6 cut(s) 214, 247, 699, 729, 1160, 1268
MaeI CTAG 2 cut(s) 209, 1451
MaeII ACGT 4 cut(s) 677, 987, 1044, 1347
MaeIII GTNAC 8 cut(s) 301, 611, 628, 1063, 1161, 1294, 1366, 1435
MalI GATC 7 cut(s) 326, 362, 541, 546, 1143, 1225, 1284
MboI GATC 7 cut(s) 324, 360, 539, 544, 1141, 1223, 1282
MboII GAAGA 5 cut(s) 224, 365, 903, 1003, 1006
MfeI CAATTG 2 cut(s) 585, 1362
MflI RGATCY 2 cut(s) 539, 1282
MhlI GDGCHC 3 cut(s) 323, 1187, 1338
MluCI AATT 9 cut(s) 81, 231, 329, 585, 854, 950, 1228, 1242, 1362
MmeI TCCRAC 1 cut(s) 1195
MroI TCCGGA 1 cut(s) 203
MseI TTAA 1 cut(s) 1477
MslI CAYNNNNRTG 1 cut(s) 841
MspA1I CMGCKG 2 cut(s) 554, 752
MspI CCGG 4 cut(s) 204, 261, 536, 1002
MspR9I CCNGG 5 cut(s) 536, 537, 672, 1002, 1119
MunI CAATTG 2 cut(s) 585, 1362
MvaI CCWGG 2 cut(s) 672, 1119
MvnI CGCG 1 cut(s) 1094
NciI CCSGG 3 cut(s) 536, 537, 1002
NcoI CCATGG 1 cut(s) 1025
NdeI CATATG 1 cut(s) 956
NdeII GATC 7 cut(s) 324, 360, 539, 544, 1141, 1223, 1282
NlaIV GGNNCC 2 cut(s) 761, 1284
NmuCI GTSAC 4 cut(s) 1063, 1161, 1294, 1366
NspI RCATGY 1 cut(s) 1302
PagI TCATGA 1 cut(s) 516
PciSI GCTCTTC 1 cut(s) 378
PcsI WCGNNNNNNNCGW 1 cut(s) 1232
PfeI GAWTC 3 cut(s) 200, 458, 908
PflMI CCANNNNNTGG 2 cut(s) 184, 1454
Ple19I CGATCG 1 cut(s) 327
Ppu21I YACGTR 1 cut(s) 678
Psp6I CCWGG 2 cut(s) 670, 1117
PspGI CCWGG 2 cut(s) 670, 1117
PspN4I GGNNCC 2 cut(s) 761, 1284
PspPI GGNCC 2 cut(s) 50, 507
PstNI CAGNNNCTG 1 cut(s) 1292
PsuI RGATCY 2 cut(s) 539, 1282
PvuI CGATCG 1 cut(s) 327
RsaI GTAC 2 cut(s) 676, 788
RsaNI GTAC 2 cut(s) 675, 787
RseI CAYNNNNRTG 1 cut(s) 841
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 1 cut(s) 1477
Sau3AI GATC 7 cut(s) 324, 360, 539, 544, 1141, 1223, 1282
Sau96I GGNCC 2 cut(s) 50, 507
ScrFI CCNGG 5 cut(s) 536, 537, 672, 1002, 1119
SduI GDGCHC 3 cut(s) 323, 1187, 1338
SfaNI GCATC 6 cut(s) 214, 247, 699, 729, 1160, 1268
SinI GGWCC 2 cut(s) 50, 507
SmaI CCCGGG 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 841
SmlI CTYRAG 3 cut(s) 105, 272, 344
SmoI CTYRAG 3 cut(s) 105, 272, 344
SnaBI TACGTA 1 cut(s) 678
SpeI ACTAGT 1 cut(s) 1450
Sse9I AATT 9 cut(s) 81, 231, 329, 585, 854, 950, 1228, 1242, 1362
SsiI CCGC 4 cut(s) 552, 752, 809, 1394
SspMI CTAG 2 cut(s) 209, 1451
StyD4I CCNGG 5 cut(s) 534, 535, 670, 1000, 1117
StyI CCWWGG 3 cut(s) 76, 1025, 1303
TaaI ACNGT 4 cut(s) 511, 1072, 1115, 1435
TaiI ACGT 4 cut(s) 680, 990, 1047, 1350
TaqI TCGA 2 cut(s) 327, 1226
TasI AATT 9 cut(s) 81, 231, 329, 585, 854, 950, 1228, 1242, 1362
TauI GCSGC 2 cut(s) 554, 755
TfiI GAWTC 3 cut(s) 200, 458, 908
Tru1I TTAA 1 cut(s) 1477
Tru9I TTAA 1 cut(s) 1477
TscAI CASTG 3 cut(s) 226, 1057, 1075
TseFI GTSAC 4 cut(s) 1063, 1161, 1294, 1366
TseI GCWGC 9 cut(s) 21, 563, 566, 575, 578, 643, 749, 878, 1127
Tsp45I GTSAC 4 cut(s) 1063, 1161, 1294, 1366
TspDTI ATGAA 6 cut(s) 505, 792, 908, 943, 951, 1235
TspGWI ACGGA 1 cut(s) 1069
TspMI CCCGGG 1 cut(s) 535
TspRI CASTG 3 cut(s) 226, 1057, 1075
Van91I CCANNNNNTGG 2 cut(s) 184, 1454
VpaK11BI GGWCC 2 cut(s) 50, 507
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 4 cut(s) 329, 854, 950, 1242
XceI RCATGY 1 cut(s) 1302
XcmI CCANNNNNNNNNTGG 1 cut(s) 1165
XmaI CCCGGG 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 2 cut(s) 209, 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.