MD13G1006300.v1.1

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003358
Physical Location & Seq
Forward (+)
394217 .. 396350
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1006300.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGATCATAGTACTAATCGTGCTTTTGGTTTGTCTGTCTTATTAGTGTGCGGAATGCTTCATGGCATTGCGTGTCAGCCGGTAGATTACCATGCTGCAGTTACCAAGTCTTTGTTGTATTTTGAGGGTCAACGCTCTGGAAAGTTGCCGGAAGATAATAAGCTGCAGAGAGTGCAATGGCGTGGAGATTCTGCACTCCAAGACGGCGCTGATGCTGGAATTGATCTGGTGGGAGGATACTATGATGCCGGTGACAATCTGAAGCTAGGGTTTCCAATGGCGTTCACAATAACAATGCTATCATGGAGCACAATAGAGATGGTCAGCCAGCTCCAGGCAAAGCAAGAGCTCACAAATGCCTTGCTCGCCATCAAGTGGGGAACCGATTACTTGATCAAAGCACATCCACAGCCGGACGTGCTCTACGCGCAAGTTGGACAGTCCAATTCTGACCATGGATGTTGGCAAAGACCGGAAGACATGACCACCCCGAGAACCTCCTTCAAAATTGATGCTCAACACCCTGGCTCCGATCTTGCTGCTGAAACTGCTGCTGCTTTTGCCGCTTCTTCAATGGCTTTCAAGAATTCGGACTCTCAATACTCGTCCACGCTTCTAATGCATGCGAAACAACTTTTTGATTTTGCCCGAGATCACCCCGGTCTTTATCAGGACAGCATCCCTTATGCCGGAAAAAGCTACAGCAGTTCCGGTTATCATGATGAAATGTTGTGGGCAGCAGCATGGCTGCATCGTGCTACAAATGAAAAACCGTACCTGGATTACGTTGAGCAAGCACAAGATATAGGGGGCACAAGATATGCATTCTCATGGGATGACAAATACCTCGGCGCACAGCTCTTGGTTGCAATGCTTGTACTGGAGGGGAAGGTTGACAATGCAGGAAAATGGGCAGAATACAGGAGCCACGCAGAGGAGTTCCTTTGTTCGTGCATCCAGAAAGGAAGCACGAACAGGAAGCGTACACCCGGAGGCTTGCTATCGTTTGCGCAGTGGGACAACATTCAATGCGTCGCCACTGCTACCTTCGCCACCATTGTTTATTCCAATTACTTGGCCGCCAAAAATGCCTCGCTCAACTGTGCTGTTGGCAACACTAACCCTTTTGATCTTGTTGCATTCGTCAAAGCACAGGTGGATTATATATTGGGGTCAAACCCCAAGAACATGAGTTACATGGTTGGATATGGAGCAAATTATCCGAAGCGGATTCATCACAGAGGAGCATCAATTATATCAATCAAGCAGGATCCGACACCGGTGACATGCGGCGGAGGGTACACTTGGTGCCACAGCAACGCACCGAACCCAAATGTGCTAGATGGAGCTGTCGTGGGCCCAGATAAAAATGATGGTTTCTCCGACGATAGAGGCAATTTTCAACTTGGTGAACCCACAACTTATAACATCGGTCCATTAGTTGGTGTCTTCGCTTACTTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

489

Amino Acids

53.46

Weight (kDa)

5.76

Isoelectric Point (pI)

28.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 30 - 483 3.5e-152 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1425
Acc16I TGCGCA 1 cut(s) 1011
AccB1I GGYRCC 1 cut(s) 1308
AccB7I CCANNNNNTGG 2 cut(s) 375, 1442
AccII CGCG 1 cut(s) 428
AciI CCGC 6 cut(s) 51, 564, 1080, 1228, 1290, 1293
AclWI GGATC 3 cut(s) 12, 1265, 1278
AcoI YGGCCR 1 cut(s) 1077
AcsI RAATTY 1 cut(s) 586
AcuI CTGAAG 1 cut(s) 281
AdeI CACNNNGTG 1 cut(s) 1308
AfaI GTAC 5 cut(s) 13, 776, 879, 985, 1301
AfiI CCNNNNNNNGG 5 cut(s) 334, 375, 689, 934, 1442
AgeI ACCGGT 1 cut(s) 1279
AgsI TTSAA 5 cut(s) 505, 573, 583, 1028, 1403
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 3 cut(s) 333, 523, 777
AluBI AGCT 7 cut(s) 163, 265, 331, 349, 699, 859, 1349
AluI AGCT 7 cut(s) 163, 265, 331, 349, 699, 859, 1349
Alw21I GWGCWC 3 cut(s) 311, 351, 423
AlwI GGATC 3 cut(s) 12, 1265, 1278
Ama87I CYCGRG 2 cut(s) 490, 648
AoxI GGCC 2 cut(s) 1077, 1357
ApaI GGGCCC 1 cut(s) 1361
ApeKI GCWGC 8 cut(s) 95, 163, 539, 551, 554, 737, 740, 748
ApoI RAATTY 1 cut(s) 586
AsiGI ACCGGT 1 cut(s) 1279
AspLEI GCGC 4 cut(s) 209, 430, 854, 1012
AspS9I GGNCC 3 cut(s) 1357, 1358, 1433
AsuC2I CCSGG 2 cut(s) 660, 990
AsuHPI GGTGA 4 cut(s) 263, 647, 1294, 1421
AvaI CYCGRG 2 cut(s) 490, 648
AvaII GGWCC 1 cut(s) 1433
BaeGI GKGCMC 2 cut(s) 815, 1361
BamHI GGATCC 1 cut(s) 1270
BanI GGYRCC 1 cut(s) 1308
BanII GRGCYC 2 cut(s) 351, 1361
BbsI GAAGAC 2 cut(s) 483, 1441
Bbv12I GWGCWC 3 cut(s) 311, 351, 423
BbvI GCAGC 8 cut(s) 82, 150, 526, 538, 541, 735, 749, 752
BccI CCATC 4 cut(s) 313, 377, 1337, 1367
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 3 cut(s) 335, 525, 779
BciVI GTATCC 1 cut(s) 230
BclI TGATCA 1 cut(s) 393
BcnI CCSGG 2 cut(s) 660, 990
BfaI CTAG 2 cut(s) 266, 1340
BfmI CTRYAG 3 cut(s) 96, 164, 700
BfoI RGCGCY 1 cut(s) 210
BfuI GTATCC 1 cut(s) 230
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 5 cut(s) 335, 525, 660, 779, 990
Bme18I GGWCC 1 cut(s) 1433
BmeT110I CYCGRG 2 cut(s) 490, 648
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 3 cut(s) 1357, 1358, 1433
BmiI GGNNCC 6 cut(s) 382, 529, 926, 1272, 1310, 1359
BmrFI CCNGG 5 cut(s) 335, 525, 660, 779, 990
BmsI GCATC 7 cut(s) 202, 235, 502, 687, 760, 963, 1256
BpiI GAAGAC 2 cut(s) 483, 1441
BpmI CTGGAG 2 cut(s) 317, 902
BpuMI CCSGG 2 cut(s) 660, 990
BsaJI CCNNGG 4 cut(s) 454, 523, 658, 847
BsaWI WCCGGW 3 cut(s) 472, 710, 1279
BsaXI ACNNNNNCTCC 4 cut(s) 512, 542, 1338, 1368
Bsc4I CCNNNNNNNGG 5 cut(s) 334, 375, 689, 934, 1442
Bse118I RCCGGY 3 cut(s) 79, 248, 1279
Bse1I ACTGG 1 cut(s) 885
Bse3DI GCAATG 3 cut(s) 66, 182, 876
BseBI CCWGG 3 cut(s) 335, 525, 779
BseDI CCNNGG 4 cut(s) 454, 523, 658, 847
BseGI GGATG 5 cut(s) 403, 464, 678, 841, 954
BseLI CCNNNNNNNGG 5 cut(s) 334, 375, 689, 934, 1442
BseMI GCAATG 3 cut(s) 66, 182, 876
BseNI ACTGG 1 cut(s) 885
BseRI GAGGAG 2 cut(s) 950, 1257
BseSI GKGCMC 2 cut(s) 815, 1361
BseXI GCAGC 8 cut(s) 82, 150, 526, 538, 541, 735, 749, 752
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 2 cut(s) 1079, 1359
BshNI GGYRCC 1 cut(s) 1308
BshTI ACCGGT 1 cut(s) 1279
BsiHKAI GWGCWC 3 cut(s) 311, 351, 423
BsiHKCI CYCGRG 2 cut(s) 490, 648
BslFI GGGAC 1 cut(s) 1031
BslI CCNNNNNNNGG 5 cut(s) 334, 375, 689, 934, 1442
BsmFI GGGAC 1 cut(s) 1031
BsmI GAATGC 3 cut(s) 60, 824, 1139
BsnI GGCC 2 cut(s) 1079, 1359
BsoBI CYCGRG 2 cut(s) 490, 648
Bsp120I GGGCCC 1 cut(s) 1357
Bsp1286I GDGCHC 5 cut(s) 311, 351, 423, 815, 1361
Bsp143I GATC 7 cut(s) 4, 223, 393, 532, 652, 1129, 1270
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 6 cut(s) 51, 564, 1080, 1228, 1290, 1293
BspANI GGCC 2 cut(s) 1079, 1359
BspFNI CGCG 1 cut(s) 428
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 6 cut(s) 382, 529, 926, 1272, 1310, 1359
BspMAI CTGCAG 2 cut(s) 100, 168
BspPI GGATC 3 cut(s) 12, 1265, 1278
BspT107I GGYRCC 1 cut(s) 1308
BsrDI GCAATG 3 cut(s) 66, 182, 876
BsrFI RCCGGY 3 cut(s) 79, 248, 1279
BsrI ACTGG 1 cut(s) 885
BssAI RCCGGY 3 cut(s) 79, 248, 1279
BssECI CCNNGG 4 cut(s) 454, 523, 658, 847
BssMI GATC 7 cut(s) 4, 223, 393, 532, 652, 1129, 1270
BssT1I CCWWGG 1 cut(s) 454
Bst2UI CCWGG 3 cut(s) 335, 525, 779
Bst4CI ACNGT 3 cut(s) 441, 774, 1103
BstAPI GCANNNNNTGC 1 cut(s) 172
BstC8I GCNNGC 5 cut(s) 329, 366, 624, 795, 998
BstDEI CTNAG 1 cut(s) 1464
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 5 cut(s) 403, 464, 678, 841, 954
BstFNI CGCG 1 cut(s) 428
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 4 cut(s) 209, 430, 854, 1012
BstKTI GATC 7 cut(s) 7, 226, 396, 535, 655, 1132, 1273
BstMBI GATC 7 cut(s) 4, 223, 393, 532, 652, 1129, 1270
BstNI CCWGG 3 cut(s) 335, 525, 779
BstNSI RCATGY 2 cut(s) 626, 1290
BstSCI CCNGG 5 cut(s) 333, 523, 658, 777, 988
BstSFI CTRYAG 3 cut(s) 96, 164, 700
BstSLI GKGCMC 2 cut(s) 815, 1361
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 8 cut(s) 82, 150, 526, 538, 541, 735, 749, 752
BstV2I GAAGAC 2 cut(s) 483, 1441
BstX2I RGATCY 1 cut(s) 1270
BstXI CCANNNNNNTGG 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1270
BsuI GTATCC 1 cut(s) 230
BsuRI GGCC 2 cut(s) 1079, 1359
BtgI CCRYGG 1 cut(s) 454
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 5 cut(s) 403, 464, 678, 841, 954
BtsI GCAGTG 2 cut(s) 1019, 1038
BtsIMutI CAGTG 2 cut(s) 1019, 1038
Cac8I GCNNGC 5 cut(s) 329, 366, 624, 795, 998
CciI TCATGA 1 cut(s) 718
CfoI GCGC 4 cut(s) 209, 430, 854, 1012
Cfr10I RCCGGY 3 cut(s) 79, 248, 1279
Cfr13I GGNCC 3 cut(s) 1357, 1358, 1433
CseI GACGC 1 cut(s) 1021
Csp6I GTAC 5 cut(s) 12, 775, 878, 984, 1300
CspAI ACCGGT 1 cut(s) 1279
CviQI GTAC 5 cut(s) 12, 775, 878, 984, 1300
DdeI CTNAG 1 cut(s) 1464
DpnI GATC 7 cut(s) 6, 225, 395, 534, 654, 1131, 1272
DpnII GATC 7 cut(s) 4, 223, 393, 532, 652, 1129, 1270
DraIII CACNNNGTG 1 cut(s) 1308
EaeI YGGCCR 1 cut(s) 1077
EciI GGCGGA 1 cut(s) 1308
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 2 cut(s) 351, 1361
Eco47I GGWCC 1 cut(s) 1433
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 2 cut(s) 490, 648
EcoICRI GAGCTC 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 586
EcoRII CCWGG 3 cut(s) 333, 523, 777
EcoT14I CCWWGG 1 cut(s) 454
EcoT22I ATGCAT 2 cut(s) 624, 826
EcoT38I GRGCYC 2 cut(s) 351, 1361
ErhI CCWWGG 1 cut(s) 454
FaqI GGGAC 1 cut(s) 1031
FbaI TGATCA 1 cut(s) 393
FokI GGATG 5 cut(s) 390, 471, 665, 848, 941
FriOI GRGCYC 2 cut(s) 351, 1361
FspBI CTAG 2 cut(s) 266, 1340
FspI TGCGCA 1 cut(s) 1011
GlaI GCGC 4 cut(s) 208, 429, 853, 1011
GsuI CTGGAG 2 cut(s) 317, 902
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 2 cut(s) 1079, 1359
HgaI GACGC 1 cut(s) 1021
HhaI GCGC 4 cut(s) 209, 430, 854, 1012
Hin6I GCGC 4 cut(s) 207, 428, 852, 1010
HinP1I GCGC 4 cut(s) 207, 428, 852, 1010
HincII GTYRAC 2 cut(s) 131, 895
HindII GTYRAC 2 cut(s) 131, 895
HinfI GANTC 3 cut(s) 188, 593, 1231
HphI GGTGA 4 cut(s) 263, 647, 1294, 1421
Hpy166II GTNNAC 7 cut(s) 131, 285, 609, 895, 986, 1302, 1412
Hpy188I TCNGA 7 cut(s) 261, 451, 532, 592, 1224, 1275, 1384
Hpy188III TCNNGA 5 cut(s) 138, 583, 671, 719, 958
Hpy8I GTNNAC 7 cut(s) 131, 285, 609, 895, 986, 1302, 1412
Hpy99I CGWCG 2 cut(s) 1037, 1388
HpyAV CCTTC 3 cut(s) 511, 883, 1057
HpyCH4III ACNGT 3 cut(s) 441, 774, 1103
HpyCH4IV ACGT 2 cut(s) 417, 786
HpyF3I CTNAG 1 cut(s) 1464
HpySE526I ACGT 2 cut(s) 417, 786
HspAI GCGC 4 cut(s) 207, 428, 852, 1010
Ksp22I TGATCA 1 cut(s) 393
Kzo9I GATC 7 cut(s) 4, 223, 393, 532, 652, 1129, 1270
LmnI GCTCC 7 cut(s) 306, 336, 533, 924, 1211, 1244, 1346
Lsp1109I GCAGC 8 cut(s) 82, 150, 526, 538, 541, 735, 749, 752
LweI GCATC 7 cut(s) 202, 235, 502, 687, 760, 963, 1256
MaeI CTAG 2 cut(s) 266, 1340
MaeII ACGT 2 cut(s) 417, 786
MaeIII GTNAC 4 cut(s) 100, 251, 1193, 1282
MalI GATC 7 cut(s) 6, 225, 395, 534, 654, 1131, 1272
MboI GATC 7 cut(s) 4, 223, 393, 532, 652, 1129, 1270
MboII GAAGA 4 cut(s) 164, 488, 561, 1441
MflI RGATCY 1 cut(s) 1270
MhlI GDGCHC 5 cut(s) 311, 351, 423, 815, 1361
MluCI AATT 8 cut(s) 219, 445, 507, 586, 1069, 1216, 1251, 1396
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 4 cut(s) 415, 1183, 1298, 1407
Mph1103I ATGCAT 2 cut(s) 624, 826
MslI CAYNNNNRTG 1 cut(s) 829
MspR9I CCNGG 5 cut(s) 335, 525, 660, 779, 990
Mva1269I GAATGC 3 cut(s) 60, 824, 1139
MvaI CCWGG 3 cut(s) 335, 525, 779
MvnI CGCG 1 cut(s) 428
NciI CCSGG 2 cut(s) 660, 990
NcoI CCATGG 1 cut(s) 454
NdeII GATC 7 cut(s) 4, 223, 393, 532, 652, 1129, 1270
NlaIV GGNNCC 6 cut(s) 382, 529, 926, 1272, 1310, 1359
NmeAIII GCCGAG 1 cut(s) 828
NmuCI GTSAC 2 cut(s) 251, 1282
NsbI TGCGCA 1 cut(s) 1011
NsiI ATGCAT 2 cut(s) 624, 826
NspI RCATGY 2 cut(s) 626, 1290
PaeI GCATGC 1 cut(s) 626
PagI TCATGA 1 cut(s) 718
PctI GAATGC 3 cut(s) 60, 824, 1139
PfeI GAWTC 2 cut(s) 188, 1231
PflMI CCANNNNNTGG 2 cut(s) 375, 1442
PinAI ACCGGT 1 cut(s) 1279
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
PsiI TTATAA 1 cut(s) 1425
Psp124BI GAGCTC 1 cut(s) 351
Psp6I CCWGG 3 cut(s) 333, 523, 777
PspGI CCWGG 3 cut(s) 333, 523, 777
PspN4I GGNNCC 6 cut(s) 382, 529, 926, 1272, 1310, 1359
PspOMI GGGCCC 1 cut(s) 1357
PspPI GGNCC 3 cut(s) 1357, 1358, 1433
PsrI GAACNNNNNNTAC 2 cut(s) 1178, 1210
PstI CTGCAG 2 cut(s) 100, 168
PsuI RGATCY 1 cut(s) 1270
RsaI GTAC 5 cut(s) 13, 776, 879, 985, 1301
RsaNI GTAC 5 cut(s) 12, 775, 878, 984, 1300
RseI CAYNNNNRTG 1 cut(s) 829
SacI GAGCTC 1 cut(s) 351
Sau3AI GATC 7 cut(s) 4, 223, 393, 532, 652, 1129, 1270
Sau96I GGNCC 3 cut(s) 1357, 1358, 1433
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 5 cut(s) 335, 525, 660, 779, 990
SduI GDGCHC 5 cut(s) 311, 351, 423, 815, 1361
SfaNI GCATC 7 cut(s) 202, 235, 502, 687, 760, 963, 1256
SfcI CTRYAG 3 cut(s) 96, 164, 700
SgrAI CRCCGGYG 1 cut(s) 1279
SinI GGWCC 1 cut(s) 1433
SmiMI CAYNNNNRTG 1 cut(s) 829
SphI GCATGC 1 cut(s) 626
Sse9I AATT 8 cut(s) 219, 445, 507, 586, 1069, 1216, 1251, 1396
SsiI CCGC 6 cut(s) 51, 564, 1080, 1228, 1290, 1293
SspMI CTAG 2 cut(s) 266, 1340
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 5 cut(s) 333, 523, 658, 777, 988
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 3 cut(s) 441, 774, 1103
TaiI ACGT 2 cut(s) 420, 789
TaqII GACCGA 1 cut(s) 1421
TasI AATT 8 cut(s) 219, 445, 507, 586, 1069, 1216, 1251, 1396
TatI WGTACW 2 cut(s) 11, 877
TauI GCSGC 3 cut(s) 566, 1082, 1293
TfiI GAWTC 2 cut(s) 188, 1231
TscAI CASTG 2 cut(s) 1019, 1045
TseFI GTSAC 2 cut(s) 251, 1282
TseI GCWGC 8 cut(s) 95, 163, 539, 551, 554, 737, 740, 748
Tsp45I GTSAC 2 cut(s) 251, 1282
TspDTI ATGAA 4 cut(s) 50, 738, 780, 1223
TspRI CASTG 2 cut(s) 1019, 1045
Van91I CCANNNNNTGG 2 cut(s) 375, 1442
VpaK11BI GGWCC 1 cut(s) 1433
XapI RAATTY 1 cut(s) 586
XceI RCATGY 2 cut(s) 626, 1290
XspI CTAG 2 cut(s) 266, 1340
ZrmI AGTACT 1 cut(s) 13
Zsp2I ATGCAT 2 cut(s) 624, 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.