Rmu_co8338879.1_g000001

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8338879.1
OG0003358
Physical Location & Seq
Forward (+)
26 .. 1001
976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8338879.1_g000001.1.cds

Sequence Viewer

Length: 686 bp
atgcagattgatttggtgggaggctactacgatgccggagacaacctcaagttgggatttccaatggccttctcagtaacaatgctgtcatggagcacgatcgaattccaaagcaagctcaaggcgaaatatgagatcgccaatgctcttcgggctatcaagtggggaacagattacttgataaaagcacatccacagcccgaagtgctttatgggcatattgggggtgttgattcagaccatcaatgttggcagagaccagaggacatgaccaccccgaggaccgtgttcatgattgatgaccaacacccgggatctgatctcgccgctgaaactgctgctgctttggctgctgcctcaattgcttttgaacgatttgatagaagttactcatcccaactcgtaactcatgcaaagcagctatttgattttgcacgcaatcatcctggtacgtatcaaaacagcatccctgaggcaggaaaagtctacgccagtagtgggtatcaggacgagatgctgtgggcagcggcatggctccatcgtgctactaatgaacagaagtacctcaacttcctaagcgaagcgggagatataggtggaacaagaacaacattctcatgggatgacaaattcgtcggcgcacaggttttggcagcaatggtacgcctatattcttcaaatccatc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

229

Amino Acids

25.51

Weight (kDa)

5.44

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 632
AccI GTMKAC 1 cut(s) 486
AciI CCGC 3 cut(s) 327, 527, 584
AclWI GGATC 1 cut(s) 322
AcsI RAATTY 2 cut(s) 104, 629
AfaI GTAC 3 cut(s) 451, 563, 663
AfiI CCNNNNNNNGG 5 cut(s) 52, 279, 310, 476, 498
AgsI TTSAA 2 cut(s) 371, 678
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 2 cut(s) 118, 421
AluI AGCT 2 cut(s) 118, 421
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 2 cut(s) 33, 250
AlwI GGATC 1 cut(s) 322
Ama87I CYCGRG 2 cut(s) 277, 310
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 7 cut(s) 338, 341, 350, 353, 418, 524, 653
ApoI RAATTY 2 cut(s) 104, 629
AspLEI GCGC 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 282
AsuC2I CCSGG 2 cut(s) 311, 312
AvaI CYCGRG 2 cut(s) 277, 310
AvaII GGWCC 1 cut(s) 282
AxyI CCTNAGG 1 cut(s) 471
BaeI ACNNNNGTAYC 2 cut(s) 545, 578
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 7 cut(s) 325, 328, 337, 340, 430, 536, 665
BccI CCATC 2 cut(s) 249, 546
BciT130I CCWGG 1 cut(s) 447
BcnI CCSGG 2 cut(s) 311, 312
BcoDI GTCTC 2 cut(s) 33, 250
BisI GCNGC 9 cut(s) 327, 339, 342, 351, 354, 419, 525, 528, 654
BlsI GCNGC 9 cut(s) 328, 340, 343, 352, 355, 420, 526, 529, 655
Bme1390I CCNGG 3 cut(s) 311, 312, 447
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 2 cut(s) 277, 310
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 536
BmrFI CCNGG 3 cut(s) 311, 312, 447
BmsI GCATC 3 cut(s) 22, 474, 504
BplI GAGNNNNNCTC 2 cut(s) 30, 62
Bpu10I CCTNAGC 1 cut(s) 575
BpuEI CTTGAG 2 cut(s) 32, 104
BpuMI CCSGG 2 cut(s) 311, 312
BsaAI YACGTR 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 278, 310
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 279, 310, 476, 498
Bse1I ACTGG 1 cut(s) 492
Bse21I CCTNAGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 663
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 278, 310
BseGI GGATG 5 cut(s) 190, 392, 442, 465, 628
BseLI CCNNNNNNNGG 5 cut(s) 52, 279, 310, 476, 498
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 2 cut(s) 87, 462
BseNI ACTGG 1 cut(s) 492
BseXI GCAGC 7 cut(s) 325, 328, 337, 340, 430, 536, 665
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 1 cut(s) 68
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 2 cut(s) 277, 310
BsiSI CCGG 2 cut(s) 36, 311
BslI CCNNNNNNNGG 5 cut(s) 52, 279, 310, 476, 498
BsmAI GTCTC 2 cut(s) 33, 250
BsnI GGCC 1 cut(s) 68
Bso31I GGTCTC 1 cut(s) 250
BsoBI CYCGRG 2 cut(s) 277, 310
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 4 cut(s) 99, 135, 314, 319
BspACI CCGC 3 cut(s) 327, 527, 584
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 2 cut(s) 86, 463
BspHI TCATGA 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 536
BspPI GGATC 1 cut(s) 322
BspQI GCTCTTC 1 cut(s) 153
BspTNI GGTCTC 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 663
BsrI ACTGG 1 cut(s) 492
BssECI CCNNGG 2 cut(s) 278, 310
BssMI GATC 4 cut(s) 99, 135, 314, 319
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 453
BstC8I GCNNGC 2 cut(s) 116, 436
BstDEI CTNAG 3 cut(s) 73, 471, 575
BstENI CCTNNNNNAGG 1 cut(s) 474
BstF5I GGATG 5 cut(s) 190, 392, 442, 465, 628
BstHHI GCGC 1 cut(s) 641
BstKTI GATC 4 cut(s) 102, 138, 317, 322
BstMAI GTCTC 2 cut(s) 33, 250
BstMBI GATC 4 cut(s) 99, 135, 314, 319
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 7 cut(s) 152, 205, 214, 335, 347, 350, 362
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 3 cut(s) 309, 310, 445
BstSNI TACGTA 1 cut(s) 453
BstV1I GCAGC 7 cut(s) 325, 328, 337, 340, 430, 536, 665
BstX2I RGATCY 1 cut(s) 314
BstYI RGATCY 1 cut(s) 314
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 1 cut(s) 68
BtsCI GGATG 5 cut(s) 190, 392, 442, 465, 628
Cac8I GCNNGC 2 cut(s) 116, 436
CciI TCATGA 1 cut(s) 291
CfoI GCGC 1 cut(s) 641
Cfr13I GGNCC 1 cut(s) 282
Cfr9I CCCGGG 1 cut(s) 310
Csp6I GTAC 3 cut(s) 450, 562, 662
CviAII CATG 6 cut(s) 90, 268, 292, 410, 531, 618
CviJI RGCY 8 cut(s) 24, 68, 118, 155, 199, 350, 421, 535
CviKI_1 RGCY 8 cut(s) 24, 68, 118, 155, 199, 350, 421, 535
CviQI GTAC 3 cut(s) 450, 562, 662
DdeI CTNAG 3 cut(s) 73, 471, 575
DpnI GATC 4 cut(s) 101, 137, 316, 321
DpnII GATC 4 cut(s) 99, 135, 314, 319
DrdI GACNNNNNNGTC 1 cut(s) 632
DseDI GACNNNNNNGTC 1 cut(s) 632
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco105I TACGTA 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 282
Eco81I CCTNAGG 1 cut(s) 471
Eco88I CYCGRG 2 cut(s) 277, 310
EcoNI CCTNNNNNAGG 1 cut(s) 474
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 445
FaeI CATG 6 cut(s) 93, 271, 295, 413, 534, 621
FatI CATG 6 cut(s) 89, 267, 291, 409, 530, 617
FauI CCCGC 1 cut(s) 577
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 9 cut(s) 327, 339, 342, 351, 354, 419, 525, 528, 654
FokI GGATG 5 cut(s) 177, 379, 429, 452, 635
Fsp4HI GCNGC 9 cut(s) 327, 339, 342, 351, 354, 419, 525, 528, 654
GlaI GCGC 1 cut(s) 640
GluI GCNGC 9 cut(s) 327, 339, 342, 351, 354, 419, 525, 528, 654
HaeIII GGCC 1 cut(s) 68
HapII CCGG 2 cut(s) 36, 311
HhaI GCGC 1 cut(s) 641
Hin1II CATG 6 cut(s) 93, 271, 295, 413, 534, 621
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HinfI GANTC 1 cut(s) 233
HpaII CCGG 2 cut(s) 36, 311
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 2 cut(s) 238, 319
Hpy188III TCNNGA 2 cut(s) 292, 506
Hpy8I GTNNAC 1 cut(s) 487
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 3 cut(s) 4, 413, 434
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 205, 214, 335, 347, 350, 362
HpyF3I CTNAG 3 cut(s) 73, 471, 575
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 6 cut(s) 93, 271, 295, 413, 534, 621
HspAI GCGC 1 cut(s) 639
Kzo9I GATC 4 cut(s) 99, 135, 314, 319
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 2 cut(s) 93, 540
Lsp1109I GCAGC 7 cut(s) 325, 328, 337, 340, 430, 536, 665
LweI GCATC 3 cut(s) 22, 474, 504
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 3 cut(s) 76, 386, 403
MalI GATC 4 cut(s) 101, 137, 316, 321
MboI GATC 4 cut(s) 99, 135, 314, 319
MboII GAAGA 2 cut(s) 140, 666
MfeI CAATTG 1 cut(s) 360
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 3 cut(s) 104, 360, 629
MnlI CCTC 7 cut(s) 14, 56, 256, 273, 367, 466, 575
MslI CAYNNNNRTG 1 cut(s) 616
MspA1I CMGCKG 2 cut(s) 329, 527
MspI CCGG 2 cut(s) 36, 311
MspR9I CCNGG 3 cut(s) 311, 312, 447
MunI CAATTG 1 cut(s) 360
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 7 cut(s) 152, 205, 214, 335, 347, 350, 362
NciI CCSGG 2 cut(s) 311, 312
NdeII GATC 4 cut(s) 99, 135, 314, 319
NlaIII CATG 6 cut(s) 93, 271, 295, 413, 534, 621
NlaIV GGNNCC 1 cut(s) 536
PagI TCATGA 1 cut(s) 291
PciSI GCTCTTC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 9 cut(s) 328, 340, 343, 352, 355, 420, 526, 529, 655
Ple19I CGATCG 1 cut(s) 102
Ppu21I YACGTR 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 536
PspPI GGNCC 1 cut(s) 282
PsuI RGATCY 1 cut(s) 314
PvuI CGATCG 1 cut(s) 102
RsaI GTAC 3 cut(s) 451, 563, 663
RsaNI GTAC 3 cut(s) 450, 562, 662
RseI CAYNNNNRTG 1 cut(s) 616
SapI GCTCTTC 1 cut(s) 153
SatI GCNGC 9 cut(s) 327, 339, 342, 351, 354, 419, 525, 528, 654
Sau3AI GATC 4 cut(s) 99, 135, 314, 319
Sau96I GGNCC 1 cut(s) 282
ScrFI CCNGG 3 cut(s) 311, 312, 447
SduI GDGCHC 1 cut(s) 98
SetI ASST 7 cut(s) 48, 120, 423, 455, 567, 598, 648
SfaNI GCATC 3 cut(s) 22, 474, 504
SinI GGWCC 1 cut(s) 282
SmaI CCCGGG 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 616
SmlI CTYRAG 2 cut(s) 47, 119
SmoI CTYRAG 2 cut(s) 47, 119
SnaBI TACGTA 1 cut(s) 453
Sse9I AATT 3 cut(s) 104, 360, 629
SsiI CCGC 3 cut(s) 327, 527, 584
StyD4I CCNGG 3 cut(s) 309, 310, 445
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 455
TaqI TCGA 1 cut(s) 102
TasI AATT 3 cut(s) 104, 360, 629
TauI GCSGC 2 cut(s) 329, 530
TfiI GAWTC 1 cut(s) 233
TseI GCWGC 7 cut(s) 338, 341, 350, 353, 418, 524, 653
TspDTI ATGAA 2 cut(s) 280, 567
TspMI CCCGGG 1 cut(s) 310
VpaK11BI GGWCC 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 474
XapI RAATTY 2 cut(s) 104, 629
XmaI CCCGGG 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.