Rh4BG443900

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003358
Physical Location & Seq
Reverse (-)
58383905 .. 58385500
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG443900.1

Sequence Viewer

Length: 1011 bp
ATGCATGAGATCGCTAATGCTCTTCGTGCTATCAAGTGGGGAACAGATTACTTGATGAAAGCACATCCACAGCCCGAAGAGCTTTATGGGCAAATTGGGGGTGTTGATTCAGACCATCAATGTTGGCAGAGACCAGAGGACATGACCACCCCGAGGACCGTGTTCATGATTGATGACCAACACCCGGGATCTGATCTCGCCGCTGAAACTGCTGCTGCTTTGGCTGCTGCCTCAATTTCTTTAGAACGTTTTGATAGAAGTTACTCATCCCAACTCGTAACTCATGCAAAGCAGCTATTTGATTTTGCCCGCAATCATCCTGGTAATTATCAAAACAGCATCCCTGAGGCAGGAAAAGTCTACGCCAGTAGTGGGTATCAGGACGAGATGCTGTGGGCAGCGGCATGGCTCCATCGTGCTACTAATGAACAGAAGTACCTCAACTTCCTAAGCGAAGCCGGAGATATAGGTGGAACAAGAACAACATTCTCATGGGATGACAAATTCGTTGGCGCACAGGTTTTGGCTGCAATGAAAGGAAACGACAACGTGAAGAAGACACCCGGAGGCTTGCTTTGGTTTCTGCCATGGAATAACAATCAATACGTTGCCACTGCTACATTTGTCATCACTGTTTATTCTATTTATTTGGACGCAAAAAAGGCCTCCATCAAGTGTCCTGGTGGCGCTGCTCACCCCTCTGATCTCATTGCATCTGCCAAGTCACAGGTGGATTACATCTTGGGATTAAACCCGAAAAACATGAGCTACATGGTTGGATATGGATCGAATTATCCGAAGCAAATTCATCACAGAGGAGCATCAATCGTCTCAATCAAGAAGGATCCAGCTCCTGTGACATGCCAAGGCGGGTTTGACTGGTTTCACAAACCCGAGCCCAACCCGAACGTGCTGGAGGGAGCAATTGTGACCCCAGATGAAAATGATGGTTTTGCGGACGACAGGAACAACTTTCAGTTAGCTGAACGACCGTTACTAATGCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

336

Amino Acids

37.21

Weight (kDa)

5.82

Isoelectric Point (pI)

28.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 3 - 329 9.2e-100 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 360
AciI CCGC 5 cut(s) 201, 310, 401, 870, 956
AclI AACGTT 1 cut(s) 247
AclWI GGATC 4 cut(s) 196, 793, 839, 852
AcsI RAATTY 2 cut(s) 503, 804
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 4 cut(s) 153, 184, 350, 372
AjnI CCWGG 2 cut(s) 319, 679
AluBI AGCT 5 cut(s) 82, 295, 768, 851, 983
AluI AGCT 5 cut(s) 82, 295, 768, 851, 983
Alw26I GTCTC 2 cut(s) 124, 835
AlwI GGATC 4 cut(s) 196, 793, 839, 852
AlwNI CAGNNNCTG 1 cut(s) 854
Ama87I CYCGRG 3 cut(s) 151, 184, 893
AoxI GGCC 1 cut(s) 663
ApeKI GCWGC 8 cut(s) 212, 215, 224, 227, 292, 398, 527, 689
ApoI RAATTY 2 cut(s) 503, 804
AspLEI GCGC 2 cut(s) 515, 689
AspS9I GGNCC 1 cut(s) 156
AsuC2I CCSGG 3 cut(s) 185, 186, 564
AsuHPI GGTGA 1 cut(s) 686
AvaI CYCGRG 3 cut(s) 151, 184, 893
AvaII GGWCC 1 cut(s) 156
AxyI CCTNAGG 1 cut(s) 345
BaeI ACNNNNGTAYC 2 cut(s) 419, 452
BamHI GGATCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 900
BarI GAAGNNNNNNTAC 2 cut(s) 428, 460
BbsI GAAGAC 1 cut(s) 563
BbvI GCAGC 8 cut(s) 199, 202, 211, 214, 304, 410, 514, 676
BccI CCATC 4 cut(s) 123, 420, 677, 941
BciT130I CCWGG 2 cut(s) 321, 681
BcnI CCSGG 3 cut(s) 185, 186, 564
BcoDI GTCTC 2 cut(s) 124, 835
BfaI CTAG 1 cut(s) 1009
BfoI RGCGCY 1 cut(s) 690
Bme1390I CCNGG 5 cut(s) 185, 186, 321, 564, 681
Bme18I GGWCC 1 cut(s) 156
BmeT110I CYCGRG 3 cut(s) 151, 184, 893
BmgT120I GGNCC 1 cut(s) 156
BmiI GGNNCC 2 cut(s) 410, 846
BmrFI CCNGG 5 cut(s) 185, 186, 321, 564, 681
BmsI GCATC 4 cut(s) 348, 378, 722, 830
BpiI GAAGAC 1 cut(s) 563
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 1 cut(s) 449
BpuMI CCSGG 3 cut(s) 185, 186, 564
BsaBI GATNNNNATC 1 cut(s) 784
BsaI GGTCTC 1 cut(s) 124
BsaJI CCNNGG 4 cut(s) 152, 184, 587, 865
BsaXI ACNNNNNCTCC 3 cut(s) 912, 942, 987
Bsc4I CCNNNNNNNGG 4 cut(s) 153, 184, 350, 372
Bse1I ACTGG 2 cut(s) 366, 884
Bse21I CCTNAGG 1 cut(s) 345
Bse3DI GCAATG 2 cut(s) 537, 708
Bse8I GATNNNNATC 1 cut(s) 784
BseBI CCWGG 2 cut(s) 321, 681
BseDI CCNNGG 4 cut(s) 152, 184, 587, 865
BseGI GGATG 5 cut(s) 64, 266, 316, 339, 502
BseJI GATNNNNATC 1 cut(s) 784
BseLI CCNNNNNNNGG 4 cut(s) 153, 184, 350, 372
BseMI GCAATG 2 cut(s) 537, 708
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 2 cut(s) 366, 884
BseRI GAGGAG 1 cut(s) 831
BseXI GCAGC 8 cut(s) 199, 202, 211, 214, 304, 410, 514, 676
Bsh1285I CGRYCG 1 cut(s) 992
BshFI GGCC 1 cut(s) 665
BsiEI CGRYCG 1 cut(s) 992
BsiHKCI CYCGRG 3 cut(s) 151, 184, 893
BsiSI CCGG 3 cut(s) 185, 459, 564
BslI CCNNNNNNNGG 4 cut(s) 153, 184, 350, 372
BsmAI GTCTC 2 cut(s) 124, 835
BsmBI CGTCTC 1 cut(s) 835
BsnI GGCC 1 cut(s) 665
Bso31I GGTCTC 1 cut(s) 124
BsoBI CYCGRG 3 cut(s) 151, 184, 893
Bsp1286I GDGCHC 1 cut(s) 900
Bsp143I GATC 6 cut(s) 9, 188, 193, 703, 785, 844
Bsp19I CCATGG 1 cut(s) 587
BspACI CCGC 5 cut(s) 201, 310, 401, 870, 956
BspANI GGCC 1 cut(s) 665
BspCNI CTCAG 1 cut(s) 337
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 410, 846
BspPI GGATC 4 cut(s) 196, 793, 839, 852
BspQI GCTCTTC 2 cut(s) 27, 72
BspTNI GGTCTC 1 cut(s) 124
BsrDI GCAATG 2 cut(s) 537, 708
BsrI ACTGG 2 cut(s) 366, 884
BssECI CCNNGG 4 cut(s) 152, 184, 587, 865
BssMI GATC 6 cut(s) 9, 188, 193, 703, 785, 844
BssT1I CCWWGG 2 cut(s) 587, 865
Bst2UI CCWGG 2 cut(s) 321, 681
Bst4CI ACNGT 3 cut(s) 160, 634, 993
Bst6I CTCTTC 2 cut(s) 27, 72
BstC8I GCNNGC 2 cut(s) 310, 572
BstDEI CTNAG 2 cut(s) 345, 449
BstDSI CCRYGG 1 cut(s) 587
BstENI CCTNNNNNAGG 1 cut(s) 348
BstF5I GGATG 5 cut(s) 64, 266, 316, 339, 502
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 2 cut(s) 515, 689
BstKTI GATC 6 cut(s) 12, 191, 196, 706, 788, 847
BstMAI GTCTC 2 cut(s) 124, 835
BstMBI GATC 6 cut(s) 9, 188, 193, 703, 785, 844
BstMCI CGRYCG 1 cut(s) 992
BstMWI GCNNNNNNNGC 7 cut(s) 26, 79, 88, 209, 221, 224, 662
BstNI CCWGG 2 cut(s) 321, 681
BstNSI RCATGY 1 cut(s) 864
BstSCI CCNGG 5 cut(s) 183, 184, 319, 562, 679
BstV1I GCAGC 8 cut(s) 199, 202, 211, 214, 304, 410, 514, 676
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 2 cut(s) 188, 844
BstYI RGATCY 2 cut(s) 188, 844
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 1 cut(s) 665
BtgI CCRYGG 1 cut(s) 587
BtsCI GGATG 5 cut(s) 64, 266, 316, 339, 502
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 2 cut(s) 612, 630
Cac8I GCNNGC 2 cut(s) 310, 572
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 1 cut(s) 165
CfoI GCGC 2 cut(s) 515, 689
Cfr13I GGNCC 1 cut(s) 156
Cfr9I CCCGGG 1 cut(s) 184
CseI GACGC 1 cut(s) 662
Csp6I GTAC 1 cut(s) 436
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 2 cut(s) 345, 449
DpnI GATC 6 cut(s) 11, 190, 195, 705, 787, 846
DpnII GATC 6 cut(s) 9, 188, 193, 703, 785, 844
Eam1104I CTCTTC 2 cut(s) 27, 72
EarI CTCTTC 2 cut(s) 27, 72
Eco130I CCWWGG 2 cut(s) 587, 865
Eco147I AGGCCT 1 cut(s) 665
Eco24I GRGCYC 1 cut(s) 900
Eco31I GGTCTC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 156
Eco81I CCTNAGG 1 cut(s) 345
Eco88I CYCGRG 3 cut(s) 151, 184, 893
EcoNI CCTNNNNNAGG 1 cut(s) 348
EcoRII CCWGG 2 cut(s) 319, 679
EcoT14I CCWWGG 2 cut(s) 587, 865
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 900
ErhI CCWWGG 2 cut(s) 587, 865
Esp3I CGTCTC 1 cut(s) 835
FauI CCCGC 2 cut(s) 317, 863
FblI GTMKAC 1 cut(s) 360
FokI GGATG 5 cut(s) 51, 253, 303, 326, 509
FriOI GRGCYC 1 cut(s) 900
FspBI CTAG 1 cut(s) 1009
GlaI GCGC 2 cut(s) 514, 688
GsuI CTGGAG 1 cut(s) 935
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 1 cut(s) 665
HapII CCGG 3 cut(s) 185, 459, 564
HgaI GACGC 1 cut(s) 662
HhaI GCGC 2 cut(s) 515, 689
Hin6I GCGC 2 cut(s) 513, 687
HinP1I GCGC 2 cut(s) 513, 687
HinfI GANTC 1 cut(s) 107
HpaII CCGG 3 cut(s) 185, 459, 564
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 4 cut(s) 112, 193, 703, 798
Hpy188III TCNNGA 3 cut(s) 166, 380, 838
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 1 cut(s) 835
HpyCH4III ACNGT 3 cut(s) 160, 634, 993
HpyCH4IV ACGT 4 cut(s) 247, 549, 606, 909
HpyCH4V TGCA 4 cut(s) 4, 287, 530, 713
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 79, 88, 209, 221, 224, 662
HpyF3I CTNAG 2 cut(s) 345, 449
HpySE526I ACGT 4 cut(s) 247, 549, 606, 909
HspAI GCGC 2 cut(s) 513, 687
Kzo9I GATC 6 cut(s) 9, 188, 193, 703, 785, 844
LguI GCTCTTC 2 cut(s) 27, 72
LmnI GCTCC 5 cut(s) 414, 818, 856, 920, 1008
Lsp1109I GCAGC 8 cut(s) 199, 202, 211, 214, 304, 410, 514, 676
LweI GCATC 4 cut(s) 348, 378, 722, 830
MaeI CTAG 1 cut(s) 1009
MaeII ACGT 4 cut(s) 247, 549, 606, 909
MaeIII GTNAC 6 cut(s) 260, 277, 723, 856, 928, 993
MalI GATC 6 cut(s) 11, 190, 195, 705, 787, 846
MboI GATC 6 cut(s) 9, 188, 193, 703, 785, 844
MboII GAAGA 4 cut(s) 14, 89, 565, 568
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 2 cut(s) 188, 844
MhlI GDGCHC 1 cut(s) 900
MluCI AATT 7 cut(s) 93, 234, 325, 503, 790, 804, 924
MmeI TCCRAC 1 cut(s) 757
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 490
MspA1I CMGCKG 2 cut(s) 203, 401
MspI CCGG 3 cut(s) 185, 459, 564
MspR9I CCNGG 5 cut(s) 185, 186, 321, 564, 681
MunI CAATTG 1 cut(s) 924
MvaI CCWGG 2 cut(s) 321, 681
MwoI GCNNNNNNNGC 7 cut(s) 26, 79, 88, 209, 221, 224, 662
NciI CCSGG 3 cut(s) 185, 186, 564
NcoI CCATGG 1 cut(s) 587
NdeII GATC 6 cut(s) 9, 188, 193, 703, 785, 844
NlaIV GGNNCC 2 cut(s) 410, 846
NmuCI GTSAC 3 cut(s) 723, 856, 928
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 864
PagI TCATGA 1 cut(s) 165
PceI AGGCCT 1 cut(s) 665
PciSI GCTCTTC 2 cut(s) 27, 72
PcsI WCGNNNNNNNCGW 1 cut(s) 794
PfeI GAWTC 1 cut(s) 107
Psp1406I AACGTT 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 319, 679
PspGI CCWGG 2 cut(s) 319, 679
PspN4I GGNNCC 2 cut(s) 410, 846
PspPI GGNCC 1 cut(s) 156
PsrI GAACNNNNNNTAC 2 cut(s) 978, 1010
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 2 cut(s) 188, 844
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 1 cut(s) 490
SapI GCTCTTC 2 cut(s) 27, 72
SaqAI TTAA 1 cut(s) 749
Sau3AI GATC 6 cut(s) 9, 188, 193, 703, 785, 844
Sau96I GGNCC 1 cut(s) 156
ScrFI CCNGG 5 cut(s) 185, 186, 321, 564, 681
SduI GDGCHC 1 cut(s) 900
SfaNI GCATC 4 cut(s) 348, 378, 722, 830
SinI GGWCC 1 cut(s) 156
SmaI CCCGGG 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 490
Sse9I AATT 7 cut(s) 93, 234, 325, 503, 790, 804, 924
SseBI AGGCCT 1 cut(s) 665
SsiI CCGC 5 cut(s) 201, 310, 401, 870, 956
SspMI CTAG 1 cut(s) 1009
StuI AGGCCT 1 cut(s) 665
StyD4I CCNGG 5 cut(s) 183, 184, 319, 562, 679
StyI CCWWGG 2 cut(s) 587, 865
TaaI ACNGT 3 cut(s) 160, 634, 993
TaiI ACGT 4 cut(s) 250, 552, 609, 912
TaqI TCGA 1 cut(s) 788
TasI AATT 7 cut(s) 93, 234, 325, 503, 790, 804, 924
TauI GCSGC 2 cut(s) 203, 404
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TscAI CASTG 2 cut(s) 619, 637
TseFI GTSAC 3 cut(s) 723, 856, 928
TseI GCWGC 8 cut(s) 212, 215, 224, 227, 292, 398, 527, 689
Tsp45I GTSAC 3 cut(s) 723, 856, 928
TspDTI ATGAA 6 cut(s) 71, 154, 441, 548, 797, 954
TspMI CCCGGG 1 cut(s) 184
TspRI CASTG 2 cut(s) 619, 637
VpaK11BI GGWCC 1 cut(s) 156
XagI CCTNNNNNAGG 1 cut(s) 348
XapI RAATTY 2 cut(s) 503, 804
XceI RCATGY 1 cut(s) 864
XcmI CCANNNNNNNNNTGG 1 cut(s) 727
XmaI CCCGGG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 1 cut(s) 1009
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.