AT2G44640

LOCATED IN mitochondrion, chloroplast, plasma membrane, plastid, chloroplast envelope

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002739
Physical Location & Seq
Forward (+)
18417243 .. 18419396
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44640.1

Sequence Viewer

Length: 1356 bp
ATGGCGAATCTTAACTCAGCTATTGATTCAGTGTTTTGGGATCAGAACGTTTCTTCACCTCAAACCCTTGAAGGCACTGCTCGCTCTGTTCCCGGTGAGCCATTTCCCTTAGACGGAGCACGAGCAAGCCGCTCTCACCGGATTCAACAGCTCTCTCTGCTTCGCGAAGGCTTTCCTCTTGGAATCATTCCTTCTTTGGCTCCTGCTTCTGACAAGAGACTCGGCTCTTTCTCTCTCAATTCTCTCTTGCTCAGTCCTTCCTCTAACAACTGGTGGTTAGGTTTAGTTGGGCAGTTCAAACCAAAGAAGCTCTTTGCTGATATCAAAGCAGATATTAGCAATGCAGAAGAGTGGGATTTGCAAGTTGTAAAGGATACAGCAAAACACATTGTTGACAAATCCCTTTACTCTATTGGTTTATGGACTCAGATTGCGTTGGGTACTTCTTCTTCTCTCTTGCTTAGCACTGAACGTCTTGGAGATAAAAACGGACTCCGAAACAAGTTGATGCTTGTTCATCCGCTTGAGAAACATGATCTCACTGTGGAAGCAGCTTGGCCAGATTTGTTTTTGGACAATAAAGGACGTTTCTGGGATGTTCCTGAATCTTTGAATGTTGATGTCTCATCCCTTGTTCCAGAGTCAGGGGTTAGATACCGGTTTGGTCTTCATAAAAGCAGGGGTAATCCTCAGCCTGTGAATGCTGCTGGTGTTGAGAGTGGTAGTGATGCTCCTACTTCTTTGATGCCTGGTTTATGCGCCAAGGCTGCAGTTTCGTACAAGGTTAACAGAGACTTGTGGAGGCCACAAGAGAAAGAAGGCAACACAGAAGAAGAGGATAAACCTGTGTTTCTGCCTTATGATTTACGTCTCAAGGAGCCTCATGCAGCAATTTCAGGAATAGTAGGAAGCAGCTTGGCAGCTTGGATCACAGGCCGAGGCATGTTGGTTAATGGCAAGAAGCGGAGTCCGATAAGCGCAGATGTATTTGGTTCTGCTTGTTATACGTTCCAAAAAGGACGGTTTAGTAAGTTGTATGGCGATCTTACCCGAGTAGATGCTCGCGTGGACCTACCCTCAGCTTTTGCCCTTGCTAAAAAACTCTTCCATGCAAGTAGTAACAACTCAGACGACACATTGTGGTCTCCCAGACTCAACCTGATATTCCAGCAACAGGTTGCGGGTCCGATTGTTTTCAAAGTAGACTCGCAGTTTCAGGTAGGAGCTGCACGCATGGAAGATGTGATATACAGTCTGAATTACTCGTTGAGGCTTCTTGAGTCTGGCAAAATCGTGGCTTGGTATTCTCCAAAGAGAAAAGAAGGCATGATCGAGCTCCGAGTTTTTGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3769

Protein Analysis

451

Amino Acids

49.83

Weight (kDa)

8.81

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3769 PF12600 384 - 451 9.7e-06 Protein of unknown function (DUF3769)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 1203
AccII CGCG 2 cut(s) 165, 1065
AciI CCGC 4 cut(s) 130, 521, 964, 1181
AclI AACGTT 1 cut(s) 48
AclWI GGATC 2 cut(s) 48, 935
AcoI YGGCCR 1 cut(s) 557
AdeI CACNNNGTG 1 cut(s) 1140
AfaI GTAC 2 cut(s) 442, 779
AfiI CCNNNNNNNGG 3 cut(s) 113, 644, 1174
AgeI ACCGGT 1 cut(s) 657
AgsI TTSAA 5 cut(s) 71, 146, 298, 613, 1198
AjnI CCWGG 1 cut(s) 748
AluBI AGCT 9 cut(s) 20, 151, 310, 554, 915, 923, 1082, 1226, 1336
AluI AGCT 9 cut(s) 20, 151, 310, 554, 915, 923, 1082, 1226, 1336
Alw21I GWGCWC 2 cut(s) 121, 1338
Alw26I GTCTC 5 cut(s) 211, 628, 786, 875, 1149
AlwI GGATC 2 cut(s) 48, 935
Ama87I CYCGRG 1 cut(s) 1050
AoxI GGCC 3 cut(s) 557, 803, 934
ApeKI GCWGC 7 cut(s) 551, 704, 767, 887, 912, 920, 1226
AsiGI ACCGGT 1 cut(s) 657
Asp700I GAANNNNTTC 1 cut(s) 171
AspLEI GCGC 2 cut(s) 761, 980
AspS9I GGNCC 2 cut(s) 1069, 1184
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 3 cut(s) 48, 107, 128
AvaI CYCGRG 1 cut(s) 1050
AvaII GGWCC 2 cut(s) 1069, 1184
BalI TGGCCA 1 cut(s) 559
BanII GRGCYC 1 cut(s) 1338
BarI GAAGNNNNNNTAC 2 cut(s) 433, 465
BauI CACGAG 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 659
Bbv12I GWGCWC 2 cut(s) 121, 1338
BbvCI CCTCAGC 2 cut(s) 690, 1078
BbvI GCAGC 7 cut(s) 563, 691, 754, 899, 924, 932, 1213
BciT130I CCWGG 1 cut(s) 750
BciVI GTATCC 1 cut(s) 367
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 5 cut(s) 211, 628, 786, 875, 1149
BfmI CTRYAG 1 cut(s) 768
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 8 cut(s) 130, 552, 705, 768, 888, 913, 921, 1227
BlpI GCTNAGC 1 cut(s) 461
BlsI GCNGC 8 cut(s) 131, 553, 706, 769, 889, 914, 922, 1228
Bme1390I CCNGG 2 cut(s) 93, 750
Bme18I GGWCC 2 cut(s) 1069, 1184
BmeT110I CYCGRG 1 cut(s) 1050
BmgT120I GGNCC 2 cut(s) 1069, 1184
BmiI GGNNCC 3 cut(s) 201, 879, 1185
BmrFI CCNGG 2 cut(s) 93, 750
BmsI GCATC 4 cut(s) 498, 718, 735, 1048
BpiI GAAGAC 1 cut(s) 659
Bpu10I CCTNAGC 2 cut(s) 690, 1078
Bpu1102I GCTNAGC 1 cut(s) 461
BpuEI CTTGAG 3 cut(s) 545, 857, 1298
BpuMI CCSGG 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 1149
BsaJI CCNNGG 2 cut(s) 762, 937
BsaWI WCCGGW 2 cut(s) 138, 657
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 644, 1174
Bse118I RCCGGY 1 cut(s) 657
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 750
BseDI CCNNGG 2 cut(s) 762, 937
BseGI GGATG 3 cut(s) 517, 601, 626
BseLI CCNNNNNNNGG 3 cut(s) 113, 644, 1174
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 6 cut(s) 30, 265, 440, 704, 1092, 1140
BseNI ACTGG 1 cut(s) 275
BseXI GCAGC 7 cut(s) 563, 691, 754, 899, 924, 932, 1213
BsgI GTGCAG 1 cut(s) 1212
Bsh1236I CGCG 2 cut(s) 165, 1065
BshFI GGCC 3 cut(s) 559, 805, 936
BshTI ACCGGT 1 cut(s) 657
BsiHKAI GWGCWC 2 cut(s) 121, 1338
BsiHKCI CYCGRG 1 cut(s) 1050
BsiSI CCGG 3 cut(s) 93, 139, 658
BslI CCNNNNNNNGG 3 cut(s) 113, 644, 1174
BsmAI GTCTC 5 cut(s) 211, 628, 786, 875, 1149
BsmBI CGTCTC 1 cut(s) 875
BsmI GAATGC 1 cut(s) 706
BsnI GGCC 3 cut(s) 559, 805, 936
Bso31I GGTCTC 1 cut(s) 1149
BsoBI CYCGRG 1 cut(s) 1050
Bsp1286I GDGCHC 2 cut(s) 121, 1338
Bsp143I GATC 5 cut(s) 40, 535, 927, 1042, 1329
Bsp1720I GCTNAGC 1 cut(s) 461
Bsp68I TCGCGA 1 cut(s) 165
BspACI CCGC 4 cut(s) 130, 521, 964, 1181
BspANI GGCC 3 cut(s) 559, 805, 936
BspCNI CTCAG 6 cut(s) 29, 264, 439, 703, 1091, 1139
BspFNI CGCG 2 cut(s) 165, 1065
BspLI GGNNCC 3 cut(s) 201, 879, 1185
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 2 cut(s) 48, 935
BspTNI GGTCTC 1 cut(s) 1149
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 346
BsrFI RCCGGY 1 cut(s) 657
BsrI ACTGG 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 657
BssECI CCNNGG 2 cut(s) 762, 937
BssMI GATC 5 cut(s) 40, 535, 927, 1042, 1329
BssSI CACGAG 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 762
Bst2BI CACGAG 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 3 cut(s) 544, 1023, 1253
Bst6I CTCTTC 3 cut(s) 342, 828, 1109
BstC8I GCNNGC 4 cut(s) 82, 127, 1063, 1231
BstDEI CTNAG 8 cut(s) 16, 109, 251, 426, 461, 690, 1078, 1126
BstF5I GGATG 3 cut(s) 517, 601, 626
BstFNI CGCG 2 cut(s) 165, 1065
BstHHI GCGC 2 cut(s) 761, 980
BstKTI GATC 5 cut(s) 43, 538, 930, 1045, 1332
BstMAI GTCTC 5 cut(s) 211, 628, 786, 875, 1149
BstMBI GATC 5 cut(s) 40, 535, 927, 1042, 1329
BstMWI GCNNNNNNNGC 3 cut(s) 81, 157, 767
BstNI CCWGG 1 cut(s) 750
BstNSI RCATGY 1 cut(s) 946
BstSCI CCNGG 2 cut(s) 91, 748
BstSFI CTRYAG 1 cut(s) 768
BstUI CGCG 2 cut(s) 165, 1065
BstV1I GCAGC 7 cut(s) 563, 691, 754, 899, 924, 932, 1213
BstV2I GAAGAC 1 cut(s) 659
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 3 cut(s) 559, 805, 936
BtsCI GGATG 3 cut(s) 517, 601, 626
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 4 cut(s) 36, 75, 465, 540
BtuMI TCGCGA 1 cut(s) 165
Cac8I GCNNGC 4 cut(s) 82, 127, 1063, 1231
CfoI GCGC 2 cut(s) 761, 980
Cfr10I RCCGGY 1 cut(s) 657
Cfr13I GGNCC 2 cut(s) 1069, 1184
Csp6I GTAC 2 cut(s) 441, 778
CspAI ACCGGT 1 cut(s) 657
CviAII CATG 6 cut(s) 533, 884, 943, 1109, 1234, 1327
CviQI GTAC 2 cut(s) 441, 778
DdeI CTNAG 8 cut(s) 16, 109, 251, 426, 461, 690, 1078, 1126
DpnI GATC 5 cut(s) 42, 537, 929, 1044, 1331
DpnII GATC 5 cut(s) 40, 535, 927, 1042, 1329
DraIII CACNNNGTG 1 cut(s) 1140
EaeI YGGCCR 1 cut(s) 557
Eam1104I CTCTTC 3 cut(s) 342, 828, 1109
EarI CTCTTC 3 cut(s) 342, 828, 1109
Ecl136II GAGCTC 1 cut(s) 1336
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 1 cut(s) 1338
Eco31I GGTCTC 1 cut(s) 1149
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 1069, 1184
Eco53kI GAGCTC 1 cut(s) 1336
Eco88I CYCGRG 1 cut(s) 1050
EcoICRI GAGCTC 1 cut(s) 1336
EcoRII CCWGG 1 cut(s) 748
EcoRV GATATC 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 1 cut(s) 1338
ErhI CCWWGG 1 cut(s) 762
Esp3I CGTCTC 1 cut(s) 875
FaeI CATG 6 cut(s) 536, 887, 946, 1112, 1237, 1330
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FatI CATG 6 cut(s) 532, 883, 942, 1108, 1233, 1326
FauI CCCGC 1 cut(s) 1174
FblI GTMKAC 1 cut(s) 1203
Fnu4HI GCNGC 8 cut(s) 130, 552, 705, 768, 888, 913, 921, 1227
FokI GGATG 3 cut(s) 504, 608, 613
FriOI GRGCYC 1 cut(s) 1338
Fsp4HI GCNGC 8 cut(s) 130, 552, 705, 768, 888, 913, 921, 1227
GlaI GCGC 2 cut(s) 760, 979
GluI GCNGC 8 cut(s) 130, 552, 705, 768, 888, 913, 921, 1227
HaeIII GGCC 3 cut(s) 559, 805, 936
HapII CCGG 3 cut(s) 93, 139, 658
HhaI GCGC 2 cut(s) 761, 980
Hin1II CATG 6 cut(s) 536, 887, 946, 1112, 1237, 1330
Hin6I GCGC 2 cut(s) 759, 978
HinP1I GCGC 2 cut(s) 759, 978
HincII GTYRAC 2 cut(s) 394, 787
HindII GTYRAC 2 cut(s) 394, 787
HpaI GTTAAC 1 cut(s) 787
HpaII CCGG 3 cut(s) 93, 139, 658
HphI GGTGA 3 cut(s) 48, 107, 128
Hpy166II GTNNAC 4 cut(s) 394, 787, 1069, 1204
Hpy188I TCNGA 9 cut(s) 45, 211, 429, 497, 972, 1129, 1188, 1257, 1340
Hpy188III TCNNGA 5 cut(s) 164, 602, 638, 897, 1277
Hpy8I GTNNAC 4 cut(s) 394, 787, 1069, 1204
HpyAV CCTTC 6 cut(s) 65, 161, 201, 267, 812, 1316
HpyCH4III ACNGT 3 cut(s) 544, 1023, 1253
HpyCH4IV ACGT 5 cut(s) 48, 472, 586, 868, 1007
HpyCH4V TGCA 6 cut(s) 344, 361, 770, 887, 1112, 1229
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 157, 767
HpyF3I CTNAG 8 cut(s) 16, 109, 251, 426, 461, 690, 1078, 1126
HpySE526I ACGT 5 cut(s) 48, 472, 586, 868, 1007
Hsp92II CATG 6 cut(s) 536, 887, 946, 1112, 1237, 1330
HspAI GCGC 2 cut(s) 759, 978
KspAI GTTAAC 1 cut(s) 787
Kzo9I GATC 5 cut(s) 40, 535, 927, 1042, 1329
LmnI GCTCC 6 cut(s) 116, 205, 736, 877, 1223, 1341
Lsp1109I GCAGC 7 cut(s) 563, 691, 754, 899, 924, 932, 1213
LweI GCATC 4 cut(s) 498, 718, 735, 1048
MaeII ACGT 5 cut(s) 48, 472, 586, 868, 1007
MaeIII GTNAC 1 cut(s) 1118
MalI GATC 5 cut(s) 42, 537, 929, 1044, 1331
MbiI CCGCTC 1 cut(s) 132
MboI GATC 5 cut(s) 40, 535, 927, 1042, 1329
MboII GAAGA 9 cut(s) 45, 359, 438, 441, 659, 842, 845, 1096, 1250
MhlI GDGCHC 2 cut(s) 121, 1338
MlsI TGGCCA 1 cut(s) 559
MluCI AATT 3 cut(s) 238, 891, 1258
MluNI TGGCCA 1 cut(s) 559
MlyI GAGTC 8 cut(s) 213, 418, 486, 650, 976, 1146, 1199, 1289
Mox20I TGGCCA 1 cut(s) 559
MroXI GAANNNNTTC 1 cut(s) 171
MscI TGGCCA 1 cut(s) 559
MseI TTAA 3 cut(s) 12, 786, 951
Msp20I TGGCCA 1 cut(s) 559
MspI CCGG 3 cut(s) 93, 139, 658
MspR9I CCNGG 2 cut(s) 93, 750
Mva1269I GAATGC 1 cut(s) 706
MvaI CCWGG 1 cut(s) 750
MvnI CGCG 2 cut(s) 165, 1065
MwoI GCNNNNNNNGC 3 cut(s) 81, 157, 767
NciI CCSGG 1 cut(s) 93
NdeII GATC 5 cut(s) 40, 535, 927, 1042, 1329
NlaIII CATG 6 cut(s) 536, 887, 946, 1112, 1237, 1330
NlaIV GGNNCC 3 cut(s) 201, 879, 1185
NmeAIII GCCGAG 2 cut(s) 201, 962
NruI TCGCGA 1 cut(s) 165
NspI RCATGY 1 cut(s) 946
PctI GAATGC 1 cut(s) 706
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 5 cut(s) 7, 26, 142, 183, 605
PinAI ACCGGT 1 cut(s) 657
PkrI GCNGC 8 cut(s) 131, 553, 706, 769, 889, 914, 922, 1228
PleI GAGTC 8 cut(s) 213, 418, 486, 649, 975, 1146, 1199, 1288
PpsI GAGTC 8 cut(s) 213, 418, 486, 649, 975, 1146, 1199, 1288
Psp124BI GAGCTC 1 cut(s) 1338
Psp1406I AACGTT 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 748
PspGI CCWGG 1 cut(s) 748
PspN4I GGNNCC 3 cut(s) 201, 879, 1185
PspPI GGNCC 2 cut(s) 1069, 1184
PstI CTGCAG 1 cut(s) 772
RruI TCGCGA 1 cut(s) 165
RsaI GTAC 2 cut(s) 442, 779
RsaNI GTAC 2 cut(s) 441, 778
SacI GAGCTC 1 cut(s) 1338
SaqAI TTAA 3 cut(s) 12, 786, 951
SatI GCNGC 8 cut(s) 130, 552, 705, 768, 888, 913, 921, 1227
Sau3AI GATC 5 cut(s) 40, 535, 927, 1042, 1329
Sau96I GGNCC 2 cut(s) 1069, 1184
SchI GAGTC 8 cut(s) 213, 418, 486, 650, 976, 1146, 1199, 1289
ScrFI CCNGG 2 cut(s) 93, 750
SduI GDGCHC 2 cut(s) 121, 1338
SfaNI GCATC 4 cut(s) 498, 718, 735, 1048
SfcI CTRYAG 1 cut(s) 768
SinI GGWCC 2 cut(s) 1069, 1184
SmlI CTYRAG 3 cut(s) 524, 872, 1277
SmoI CTYRAG 3 cut(s) 524, 872, 1277
Sse9I AATT 3 cut(s) 238, 891, 1258
SsiI CCGC 4 cut(s) 130, 521, 964, 1181
SstI GAGCTC 1 cut(s) 1338
StyD4I CCNGG 2 cut(s) 91, 748
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 3 cut(s) 544, 1023, 1253
TaiI ACGT 5 cut(s) 51, 475, 589, 871, 1010
TaqI TCGA 1 cut(s) 1332
TasI AATT 3 cut(s) 238, 891, 1258
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 5 cut(s) 7, 26, 142, 183, 605
Tru1I TTAA 3 cut(s) 12, 786, 951
Tru9I TTAA 3 cut(s) 12, 786, 951
TscAI CASTG 4 cut(s) 36, 82, 472, 547
TseI GCWGC 7 cut(s) 551, 704, 767, 887, 912, 920, 1226
TspDTI ATGAA 2 cut(s) 506, 659
TspGWI ACGGA 2 cut(s) 129, 504
TspRI CASTG 4 cut(s) 36, 82, 472, 547
VpaK11BI GGWCC 2 cut(s) 1069, 1184
XceI RCATGY 1 cut(s) 946
XmiI GTMKAC 1 cut(s) 1203
XmnI GAANNNNTTC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.