Rmu_sc0002276.1_g000004

Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002276.1
OG0002739
Physical Location & Seq
Forward (+)
18594 .. 19148
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002276.1_g000004.1.cds

Sequence Viewer

Length: 555 bp
atgtggactactccgaacatatggctaattgtgacaaatttcatgtctggaagctttatattcttcatttatcttgctgttctaatattggggtatgtgctactgaacgatttttctcaacttctaagatgtttacagctaccttatcatgacatcacattagaggctgcatggcctgagctgttcattgaccataaaggacaatattgggatgtacccttagatctgtcatcacttgtttctgaatctggattgcggtaccgggttggtatacataaaaatagtggccatccccaggcagttaatgccgtaaatgatgaagcacctacttctctgatgcctggattgtgtgcaaaggctgccttttcttatgaaaatagcagagacctttggaggcagaaggagacacagaatgatctcatggtaaagaaagaccagggttggttttggcggccatcatatgatgtgcatctcaaagaacctcatgctgcagtatctggagtttttggtaaatttactgtcttagaagttgctcaatttatgcataatcattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3769

Protein Analysis

184

Amino Acids

21.16

Weight (kDa)

6.03

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 258
AccB1I GGYRCC 1 cut(s) 258
AccI GTMKAC 1 cut(s) 271
AciI CCGC 2 cut(s) 256, 451
AcoI YGGCCR 2 cut(s) 286, 452
AcsI RAATTY 2 cut(s) 37, 512
AfaI GTAC 2 cut(s) 216, 260
AfiI CCNNNNNNNGG 1 cut(s) 295
AjnI CCWGG 3 cut(s) 294, 340, 435
AluBI AGCT 3 cut(s) 54, 139, 181
AluI AGCT 3 cut(s) 54, 139, 181
Alw26I GTCTC 2 cut(s) 378, 398
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 3 cut(s) 173, 286, 452
ApeKI GCWGC 3 cut(s) 167, 359, 488
ApoI RAATTY 2 cut(s) 37, 512
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
Asp718I GGTACC 1 cut(s) 258
AsuC2I CCSGG 1 cut(s) 263
BalI TGGCCA 1 cut(s) 288
BanI GGYRCC 1 cut(s) 258
BbvI GCAGC 3 cut(s) 154, 346, 475
BccI CCATC 2 cut(s) 297, 463
BceAI ACGGC 1 cut(s) 293
BciT130I CCWGG 3 cut(s) 296, 342, 437
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 2 cut(s) 378, 398
BfmI CTRYAG 1 cut(s) 489
BglII AGATCT 1 cut(s) 223
BisI GCNGC 4 cut(s) 168, 360, 452, 489
BlsI GCNGC 4 cut(s) 169, 361, 453, 490
Bme1390I CCNGG 4 cut(s) 263, 296, 342, 437
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 4 cut(s) 263, 296, 342, 437
BmsI GCATC 2 cut(s) 327, 478
BpmI CTGGAG 1 cut(s) 519
Bpu10I CCTNAGC 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 263
BsaBI GATNNNNATC 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 294, 436
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse8I GATNNNNATC 1 cut(s) 468
BseBI CCWGG 3 cut(s) 296, 342, 437
BseDI CCNNGG 2 cut(s) 294, 436
BseGI GGATG 2 cut(s) 217, 289
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 168
BseXI GCAGC 3 cut(s) 154, 346, 475
BshFI GGCC 3 cut(s) 175, 288, 454
BshNI GGYRCC 1 cut(s) 258
BsiSI CCGG 1 cut(s) 262
BslI CCNNNNNNNGG 1 cut(s) 295
BsmAI GTCTC 2 cut(s) 378, 398
BsnI GGCC 3 cut(s) 175, 288, 454
Bso31I GGTCTC 1 cut(s) 378
Bsp143I GATC 2 cut(s) 223, 415
BspACI CCGC 2 cut(s) 256, 451
BspANI GGCC 3 cut(s) 175, 288, 454
BspCNI CTCAG 1 cut(s) 169
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 260
BspMAI CTGCAG 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 258
BspTNI GGTCTC 1 cut(s) 378
BssECI CCNNGG 2 cut(s) 294, 436
BssMI GATC 2 cut(s) 223, 415
BssNAI GTATAC 1 cut(s) 272
Bst1107I GTATAC 1 cut(s) 272
Bst2UI CCWGG 3 cut(s) 296, 342, 437
Bst4CI ACNGT 1 cut(s) 520
BstAPI GCANNNNNTGC 2 cut(s) 305, 359
BstDEI CTNAG 4 cut(s) 125, 177, 220, 523
BstF5I GGATG 2 cut(s) 217, 289
BstKTI GATC 2 cut(s) 226, 418
BstMAI GTCTC 2 cut(s) 378, 398
BstMBI GATC 2 cut(s) 223, 415
BstMWI GCNNNNNNNGC 2 cut(s) 305, 359
BstNI CCWGG 3 cut(s) 296, 342, 437
BstSCI CCNGG 4 cut(s) 261, 294, 340, 435
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 3 cut(s) 154, 346, 475
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BstZ17I GTATAC 1 cut(s) 272
BsuRI GGCC 3 cut(s) 175, 288, 454
BtsCI GGATG 2 cut(s) 217, 289
CaiI CAGNNNCTG 1 cut(s) 497
CciI TCATGA 1 cut(s) 148
Csp6I GTAC 2 cut(s) 215, 259
CviAII CATG 5 cut(s) 43, 149, 171, 421, 485
CviJI RGCY 9 cut(s) 25, 54, 139, 167, 175, 181, 288, 359, 454
CviKI_1 RGCY 9 cut(s) 25, 54, 139, 167, 175, 181, 288, 359, 454
CviQI GTAC 2 cut(s) 215, 259
DdeI CTNAG 4 cut(s) 125, 177, 220, 523
DpnI GATC 2 cut(s) 225, 417
DpnII GATC 2 cut(s) 223, 415
EaeI YGGCCR 2 cut(s) 286, 452
Eco31I GGTCTC 1 cut(s) 378
EcoRII CCWGG 3 cut(s) 294, 340, 435
EcoT22I ATGCAT 1 cut(s) 546
FaeI CATG 5 cut(s) 46, 152, 174, 424, 488
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FatI CATG 5 cut(s) 42, 148, 170, 420, 484
FauNDI CATATG 2 cut(s) 20, 460
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 4 cut(s) 168, 360, 452, 489
FokI GGATG 2 cut(s) 224, 276
Fsp4HI GCNGC 4 cut(s) 168, 360, 452, 489
GluI GCNGC 4 cut(s) 168, 360, 452, 489
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 3 cut(s) 175, 288, 454
HapII CCGG 1 cut(s) 262
Hin1II CATG 5 cut(s) 46, 152, 174, 424, 488
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 1 cut(s) 245
HpaII CCGG 1 cut(s) 262
Hpy166II GTNNAC 3 cut(s) 6, 134, 272
Hpy188I TCNGA 3 cut(s) 15, 244, 336
Hpy188III TCNNGA 4 cut(s) 48, 149, 249, 498
Hpy8I GTNNAC 3 cut(s) 6, 134, 272
HpyAV CCTTC 1 cut(s) 394
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4V TGCA 5 cut(s) 170, 353, 469, 491, 544
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 359
HpyF3I CTNAG 4 cut(s) 125, 177, 220, 523
Hsp92II CATG 5 cut(s) 46, 152, 174, 424, 488
KpnI GGTACC 1 cut(s) 262
Kzo9I GATC 2 cut(s) 223, 415
Lsp1109I GCAGC 3 cut(s) 154, 346, 475
LweI GCATC 2 cut(s) 327, 478
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 225, 417
MboI GATC 2 cut(s) 223, 415
MboII GAAGA 1 cut(s) 55
MflI RGATCY 1 cut(s) 223
MlsI TGGCCA 1 cut(s) 288
MluCI AATT 4 cut(s) 27, 37, 512, 536
MluNI TGGCCA 1 cut(s) 288
MnlI CCTC 3 cut(s) 157, 387, 492
Mox20I TGGCCA 1 cut(s) 288
Mph1103I ATGCAT 1 cut(s) 546
MscI TGGCCA 1 cut(s) 288
MseI TTAA 2 cut(s) 303, 553
Msp20I TGGCCA 1 cut(s) 288
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 4 cut(s) 263, 296, 342, 437
MvaI CCWGG 3 cut(s) 296, 342, 437
MwoI GCNNNNNNNGC 2 cut(s) 305, 359
NciI CCSGG 1 cut(s) 263
NdeI CATATG 2 cut(s) 20, 460
NdeII GATC 2 cut(s) 223, 415
NlaIII CATG 5 cut(s) 46, 152, 174, 424, 488
NlaIV GGNNCC 1 cut(s) 260
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 546
PagI TCATGA 1 cut(s) 148
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 4 cut(s) 169, 361, 453, 490
Psp6I CCWGG 3 cut(s) 294, 340, 435
PspGI CCWGG 3 cut(s) 294, 340, 435
PspN4I GGNNCC 1 cut(s) 260
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 497
PsuI RGATCY 1 cut(s) 223
RsaI GTAC 2 cut(s) 216, 260
RsaNI GTAC 2 cut(s) 215, 259
SaqAI TTAA 2 cut(s) 303, 553
SatI GCNGC 4 cut(s) 168, 360, 452, 489
Sau3AI GATC 2 cut(s) 223, 415
ScrFI CCNGG 4 cut(s) 263, 296, 342, 437
SetI ASST 7 cut(s) 56, 141, 145, 183, 328, 390, 484
SfaNI GCATC 2 cut(s) 327, 478
SfcI CTRYAG 1 cut(s) 489
Sse9I AATT 4 cut(s) 27, 37, 512, 536
SsiI CCGC 2 cut(s) 256, 451
SspI AATATT 2 cut(s) 87, 206
StyD4I CCNGG 4 cut(s) 261, 294, 340, 435
TaaI ACNGT 1 cut(s) 520
TasI AATT 4 cut(s) 27, 37, 512, 536
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 303, 553
Tru9I TTAA 2 cut(s) 303, 553
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 167, 359, 488
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 5 cut(s) 31, 55, 175, 333, 387
XapI RAATTY 2 cut(s) 37, 512
XmiI GTMKAC 1 cut(s) 271
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.