Rh1BG154200

Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002739
Physical Location & Seq
Forward (+)
25273279 .. 25278121
4843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG154200.1

Sequence Viewer

Length: 1056 bp
ATGCTAGTCAATAACCTTGAACATGTGGATTACTCTGAACATATGGCTAATTGTGACAAATTTCATGTCTGGAAGCTTTATATTCTTCATTTATCTTGCTGTTTTAATATTGGGTATGTGCTACTGAATGATTTTTCTCAACTTCTAAGATGTTTACAGCTACCTTATCATGACATCACACTAGAGGCTGCATGGCCTGAGCTGTTCATTGACCATAAAGGACAATATTGGGATGTACCGGAGTCAATTTCCTTAGATCTGTCATCACTTGTTTCTGAATCTGGATTGCGGTACCGGGTTGGTATAAATAAAAATAGTGGCCATCCCCAGGCAGTTAATGCCGTAAATGATGAGGCACCTACTTCTCTGATGCCTGGATTGTGTGCAAAGGCTGCCTTTTCTTATGAAAAGAGCAGAGACCTTTGGAGGCAGAAGGAGACACAGAATGATCTCATGGTAAAGAAAGACAGGGGTTGGTTTTGGCGGCCATCATATGATGTGCGTCTCAAAGAACCTCATGCTGCAGTATCTGGAATTTTTGGTGGGAGCTTTGCAGCCTGGTTTCAGGATGGGCACAGCCCAGTGGCTGTTGAACTTAGAGGAGATGGGAACACTTCTTCAAGTACTAAGAAGAGAAGTCCTGTCAGTGCTGATTTTTTTGGTTCAATTTGCTATCGCTTCCAGCATGGGAAATTTCGAGAGCTTTATGGGGATCTTACCAGGATAGATGCCCACCTGGATATTGGTTCAGCTTCAGCTCTTGCTAAAAGGGTTGTGAATGGCTTCAAGAGTTCCTCTGCTAATAGTGCTATAGATCCTATGTCCTCCCCTAGGATCAATCTAATCTTTCAACAGCAGGTTGTGGGGCCAATTGTCTTTCGAGTGGATTCAAGGGTTTCACTAGCCTCTTTGCCTGGGAAACGTGGTCCACATATTGAGGATTTCATTTACAGCTTGAGCTACTCCTTGAGGCTTTTACAGTCAGGCAAGGTTGTTGCTTGGTATTCCCCGAAAAGAAAAGAAGGAATGATTGAGTTGCGGGTGTTTGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3769

Protein Analysis

351

Amino Acids

39.68

Weight (kDa)

8.42

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 847
Acc65I GGTACC 1 cut(s) 291
AccB1I GGYRCC 2 cut(s) 291, 355
AciI CCGC 3 cut(s) 289, 484, 1039
AclWI GGATC 3 cut(s) 720, 809, 842
AcoI YGGCCR 2 cut(s) 319, 485
AcsI RAATTY 3 cut(s) 59, 534, 692
AcuI CTGAAG 1 cut(s) 738
AfaI GTAC 3 cut(s) 237, 293, 625
AfiI CCNNNNNNNGG 2 cut(s) 328, 831
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 7 cut(s) 20, 593, 621, 666, 787, 851, 891
AjnI CCWGG 6 cut(s) 327, 373, 557, 719, 735, 913
AluBI AGCT 9 cut(s) 76, 160, 202, 549, 703, 752, 758, 954, 960
AluI AGCT 9 cut(s) 76, 160, 202, 549, 703, 752, 758, 954, 960
Alw26I GTCTC 3 cut(s) 411, 431, 509
AlwI GGATC 3 cut(s) 720, 809, 842
AlwNI CAGNNNCTG 2 cut(s) 530, 587
AoxI GGCC 4 cut(s) 194, 319, 485, 866
ApeKI GCWGC 4 cut(s) 188, 392, 521, 554
ApoI RAATTY 3 cut(s) 59, 534, 692
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
Asp700I GAANNNNTTC 1 cut(s) 782
Asp718I GGTACC 1 cut(s) 291
AspA2I CCTAGG 1 cut(s) 830
AspS9I GGNCC 2 cut(s) 866, 926
AsuC2I CCSGG 1 cut(s) 296
AvaII GGWCC 1 cut(s) 926
AvrII CCTAGG 1 cut(s) 830
BaeGI GKGCMC 1 cut(s) 576
BalI TGGCCA 1 cut(s) 321
BanI GGYRCC 2 cut(s) 291, 355
BbvI GCAGC 4 cut(s) 175, 379, 508, 566
BccI CCATC 4 cut(s) 330, 496, 563, 599
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 6 cut(s) 329, 375, 559, 721, 737, 915
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 3 cut(s) 411, 431, 509
BfaI CTAG 4 cut(s) 5, 182, 831, 902
BfmI CTRYAG 2 cut(s) 522, 810
BfuAI ACCTGC 1 cut(s) 847
BglII AGATCT 1 cut(s) 256
BisI GCNGC 5 cut(s) 189, 393, 485, 522, 555
BlnI CCTAGG 1 cut(s) 830
BlsI GCNGC 5 cut(s) 190, 394, 486, 523, 556
BmcAI AGTACT 1 cut(s) 625
Bme1390I CCNGG 7 cut(s) 296, 329, 375, 559, 721, 737, 915
Bme18I GGWCC 1 cut(s) 926
BmgT120I GGNCC 2 cut(s) 866, 926
BmiI GGNNCC 3 cut(s) 293, 357, 867
BmrFI CCNGG 7 cut(s) 296, 329, 375, 559, 721, 737, 915
BmrI ACTGGG 1 cut(s) 575
BmsI GCATC 2 cut(s) 360, 718
BmuI ACTGGG 1 cut(s) 575
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 976, 988
BpuMI CCSGG 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 327, 830, 914
BsaWI WCCGGW 1 cut(s) 238
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 831
Bse1I ACTGG 1 cut(s) 581
BseBI CCWGG 6 cut(s) 329, 375, 559, 721, 737, 915
BseDI CCNNGG 3 cut(s) 327, 830, 914
BseGI GGATG 3 cut(s) 238, 322, 574
BseLI CCNNNNNNNGG 2 cut(s) 328, 831
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 581
BseRI GAGGAG 1 cut(s) 615
BseSI GKGCMC 1 cut(s) 576
BseXI GCAGC 4 cut(s) 175, 379, 508, 566
BshFI GGCC 4 cut(s) 196, 321, 487, 868
BshNI GGYRCC 2 cut(s) 291, 355
BsiSI CCGG 2 cut(s) 239, 295
BslI CCNNNNNNNGG 2 cut(s) 328, 831
BsmAI GTCTC 3 cut(s) 411, 431, 509
BsmBI CGTCTC 1 cut(s) 509
BsnI GGCC 4 cut(s) 196, 321, 487, 868
Bso31I GGTCTC 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 5 cut(s) 256, 448, 712, 814, 834
BspACI CCGC 3 cut(s) 289, 484, 1039
BspANI GGCC 4 cut(s) 196, 321, 487, 868
BspCNI CTCAG 1 cut(s) 190
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 3 cut(s) 293, 357, 867
BspMAI CTGCAG 1 cut(s) 526
BspMI ACCTGC 1 cut(s) 847
BspPI GGATC 3 cut(s) 720, 809, 842
BspT107I GGYRCC 2 cut(s) 291, 355
BspTNI GGTCTC 1 cut(s) 411
BsrI ACTGG 1 cut(s) 581
BssECI CCNNGG 3 cut(s) 327, 830, 914
BssMI GATC 5 cut(s) 256, 448, 712, 814, 834
BssT1I CCWWGG 1 cut(s) 830
Bst2UI CCWGG 6 cut(s) 329, 375, 559, 721, 737, 915
Bst4CI ACNGT 1 cut(s) 981
Bst6I CTCTTC 1 cut(s) 626
BstAPI GCANNNNNTGC 2 cut(s) 338, 392
BstDEI CTNAG 5 cut(s) 146, 198, 253, 596, 627
BstENI CCTNNNNNAGG 1 cut(s) 829
BstF5I GGATG 3 cut(s) 238, 322, 574
BstKTI GATC 5 cut(s) 259, 451, 715, 817, 837
BstMAI GTCTC 3 cut(s) 411, 431, 509
BstMBI GATC 5 cut(s) 256, 448, 712, 814, 834
BstMWI GCNNNNNNNGC 3 cut(s) 338, 392, 806
BstNI CCWGG 6 cut(s) 329, 375, 559, 721, 737, 915
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 7 cut(s) 294, 327, 373, 557, 719, 735, 913
BstSFI CTRYAG 2 cut(s) 522, 810
BstSLI GKGCMC 1 cut(s) 576
BstV1I GCAGC 4 cut(s) 175, 379, 508, 566
BstX2I RGATCY 3 cut(s) 256, 712, 814
BstYI RGATCY 3 cut(s) 256, 712, 814
BsuRI GGCC 4 cut(s) 196, 321, 487, 868
BtsCI GGATG 3 cut(s) 238, 322, 574
BtsIMutI CAGTG 2 cut(s) 588, 652
BveI ACCTGC 1 cut(s) 847
CaiI CAGNNNCTG 2 cut(s) 530, 587
CciI TCATGA 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 866, 926
CseI GACGC 1 cut(s) 491
Csp6I GTAC 3 cut(s) 236, 292, 624
CviAII CATG 7 cut(s) 23, 65, 170, 192, 454, 518, 686
CviQI GTAC 3 cut(s) 236, 292, 624
DdeI CTNAG 5 cut(s) 146, 198, 253, 596, 627
DpnI GATC 5 cut(s) 258, 450, 714, 816, 836
DpnII GATC 5 cut(s) 256, 448, 712, 814, 834
EaeI YGGCCR 2 cut(s) 319, 485
Eam1104I CTCTTC 1 cut(s) 626
EarI CTCTTC 1 cut(s) 626
Eco130I CCWWGG 1 cut(s) 830
Eco31I GGTCTC 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 926
Eco57I CTGAAG 1 cut(s) 738
EcoNI CCTNNNNNAGG 1 cut(s) 829
EcoRII CCWGG 6 cut(s) 327, 373, 557, 719, 735, 913
EcoT14I CCWWGG 1 cut(s) 830
ErhI CCWWGG 1 cut(s) 830
Esp3I CGTCTC 1 cut(s) 509
FaeI CATG 7 cut(s) 26, 68, 173, 195, 457, 521, 689
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FatI CATG 7 cut(s) 22, 64, 169, 191, 453, 517, 685
FauI CCCGC 1 cut(s) 1032
FauNDI CATATG 2 cut(s) 42, 493
Fnu4HI GCNGC 5 cut(s) 189, 393, 485, 522, 555
FokI GGATG 3 cut(s) 245, 309, 581
Fsp4HI GCNGC 5 cut(s) 189, 393, 485, 522, 555
FspBI CTAG 4 cut(s) 5, 182, 831, 902
GluI GCNGC 5 cut(s) 189, 393, 485, 522, 555
HaeIII GGCC 4 cut(s) 196, 321, 487, 868
HapII CCGG 2 cut(s) 239, 295
HgaI GACGC 1 cut(s) 491
Hin1II CATG 7 cut(s) 26, 68, 173, 195, 457, 521, 689
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 3 cut(s) 242, 278, 887
HpaII CCGG 2 cut(s) 239, 295
Hpy166II GTNNAC 2 cut(s) 155, 929
Hpy188I TCNGA 3 cut(s) 37, 277, 369
Hpy188III TCNNGA 7 cut(s) 70, 170, 282, 531, 566, 698, 787
Hpy8I GTNNAC 2 cut(s) 155, 929
HpyAV CCTTC 2 cut(s) 427, 1016
HpyCH4III ACNGT 1 cut(s) 981
HpyCH4IV ACGT 1 cut(s) 922
HpyCH4V TGCA 4 cut(s) 191, 386, 524, 554
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 392, 806
HpyF3I CTNAG 5 cut(s) 146, 198, 253, 596, 627
HpySE526I ACGT 1 cut(s) 922
Hsp92II CATG 7 cut(s) 26, 68, 173, 195, 457, 521, 689
KpnI GGTACC 1 cut(s) 295
Kzo9I GATC 5 cut(s) 256, 448, 712, 814, 834
LmnI GCTCC 1 cut(s) 546
Lsp1109I GCAGC 4 cut(s) 175, 379, 508, 566
LweI GCATC 2 cut(s) 360, 718
MaeI CTAG 4 cut(s) 5, 182, 831, 902
MaeII ACGT 1 cut(s) 922
MaeIII GTNAC 1 cut(s) 53
MalI GATC 5 cut(s) 258, 450, 714, 816, 836
MboI GATC 5 cut(s) 256, 448, 712, 814, 834
MboII GAAGA 3 cut(s) 77, 609, 643
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 3 cut(s) 256, 712, 814
MhlI GDGCHC 1 cut(s) 576
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 7 cut(s) 49, 59, 246, 534, 666, 692, 870
MluNI TGGCCA 1 cut(s) 321
MlyI GAGTC 1 cut(s) 251
Mox20I TGGCCA 1 cut(s) 321
MroXI GAANNNNTTC 1 cut(s) 782
MscI TGGCCA 1 cut(s) 321
MseI TTAA 2 cut(s) 105, 336
Msp20I TGGCCA 1 cut(s) 321
MspI CCGG 2 cut(s) 239, 295
MspR9I CCNGG 7 cut(s) 296, 329, 375, 559, 721, 737, 915
MunI CAATTG 1 cut(s) 870
MvaI CCWGG 6 cut(s) 329, 375, 559, 721, 737, 915
MwoI GCNNNNNNNGC 3 cut(s) 338, 392, 806
NciI CCSGG 1 cut(s) 296
NdeI CATATG 2 cut(s) 42, 493
NdeII GATC 5 cut(s) 256, 448, 712, 814, 834
NlaIII CATG 7 cut(s) 26, 68, 173, 195, 457, 521, 689
NlaIV GGNNCC 3 cut(s) 293, 357, 867
NmuCI GTSAC 1 cut(s) 53
NspI RCATGY 1 cut(s) 26
PagI TCATGA 1 cut(s) 169
PciI ACATGT 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 782
PfeI GAWTC 2 cut(s) 278, 887
PkrI GCNGC 5 cut(s) 190, 394, 486, 523, 556
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PscI ACATGT 1 cut(s) 22
Psp6I CCWGG 6 cut(s) 327, 373, 557, 719, 735, 913
PspGI CCWGG 6 cut(s) 327, 373, 557, 719, 735, 913
PspN4I GGNNCC 3 cut(s) 293, 357, 867
PspPI GGNCC 2 cut(s) 866, 926
PstI CTGCAG 1 cut(s) 526
PstNI CAGNNNCTG 2 cut(s) 530, 587
PsuI RGATCY 3 cut(s) 256, 712, 814
RsaI GTAC 3 cut(s) 237, 293, 625
RsaNI GTAC 3 cut(s) 236, 292, 624
SaqAI TTAA 2 cut(s) 105, 336
SatI GCNGC 5 cut(s) 189, 393, 485, 522, 555
Sau3AI GATC 5 cut(s) 256, 448, 712, 814, 834
Sau96I GGNCC 2 cut(s) 866, 926
ScaI AGTACT 1 cut(s) 625
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 7 cut(s) 296, 329, 375, 559, 721, 737, 915
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 2 cut(s) 360, 718
SfcI CTRYAG 2 cut(s) 522, 810
SinI GGWCC 1 cut(s) 926
SmlI CTYRAG 2 cut(s) 955, 967
SmoI CTYRAG 2 cut(s) 955, 967
Sse9I AATT 7 cut(s) 49, 59, 246, 534, 666, 692, 870
SsiI CCGC 3 cut(s) 289, 484, 1039
SspI AATATT 2 cut(s) 109, 227
SspMI CTAG 4 cut(s) 5, 182, 831, 902
StyD4I CCNGG 7 cut(s) 294, 327, 373, 557, 719, 735, 913
StyI CCWWGG 1 cut(s) 830
TaaI ACNGT 1 cut(s) 981
TaiI ACGT 1 cut(s) 925
TaqI TCGA 2 cut(s) 697, 880
TasI AATT 7 cut(s) 49, 59, 246, 534, 666, 692, 870
TatI WGTACW 1 cut(s) 623
TauI GCSGC 1 cut(s) 487
TfiI GAWTC 2 cut(s) 278, 887
Tru1I TTAA 2 cut(s) 105, 336
Tru9I TTAA 2 cut(s) 105, 336
TscAI CASTG 2 cut(s) 588, 652
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 4 cut(s) 188, 392, 521, 554
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 5 cut(s) 53, 77, 196, 420, 934
TspRI CASTG 2 cut(s) 588, 652
VpaK11BI GGWCC 1 cut(s) 926
XagI CCTNNNNNAGG 1 cut(s) 829
XapI RAATTY 3 cut(s) 59, 534, 692
XceI RCATGY 1 cut(s) 26
XcmI CCANNNNNNNNNTGG 1 cut(s) 740
XmaJI CCTAGG 1 cut(s) 830
XmnI GAANNNNTTC 1 cut(s) 782
XspI CTAG 4 cut(s) 5, 182, 831, 902
ZrmI AGTACT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.