AT3G07680

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004137
Physical Location & Seq
Forward (+)
2455239 .. 2456918
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07680.1

Sequence Viewer

Length: 627 bp
ATGAGCTTGAAGGGTACGATCGTATTACTAGGGCTCTTATGGAGCTTCCAGGCTACGTTAGGGATTAGATTCGTGATAGACCGTGAAGAATGTTTCTCCCACAAGGCTGAATATGAAGGTGATACGCTTCATGTCTCTTTCGTCGTCATCAAGTCTGACTCTCAATGGCATTTTAACGAGGATGGTGTAGATCTTGTGATACATGGCCCAACAGGCGAACAAATTCATGACTTCAGGGAGCAGATAAGCGCAAAGCATGATTTTGTTGTCCAAAAAAAAGGGGTTTACAGATTCTGTTTCACAAACAAGTCTCCTTATCATGAAACCATTGACTTCGATGTACAGCTTGGTCACTTTGCATACTACGATCAACACGCAAAGGACGAGCATTTCACTCCCTTGATGGAGCAAATATCAAAGTTAGAGGAGGCTCTTTACAACATCCAATTTGAACAACATTGGTTAGAAGCTCAGACCGATCGTCAAGCTATCGTGAACGAGAACATGAGCAAAAGAGCAGTACACAAAGCTTTGTTCGAGTCGTTTGCACTGATTGGGGCAAGTTTTCTCCAAGTTTATCTTCTGCGTCGCTTGTTTGAACGCAAACTCGGCATGTCTCGTGTCTAA

Protein Analysis

208

Amino Acids

24.33

Weight (kDa)

5.93

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 28 - 200 4.8e-34 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 222
AcuI CTGAAG 1 cut(s) 217
AfaI GTAC 3 cut(s) 16, 342, 522
AgsI TTSAA 3 cut(s) 10, 452, 599
AhdI GACNNNNNGTC 1 cut(s) 480
AjnI CCWGG 1 cut(s) 48
AjuI GAANNNNNNNTTGG 2 cut(s) 564, 596
AluBI AGCT 6 cut(s) 6, 45, 346, 470, 488, 530
AluI AGCT 6 cut(s) 6, 45, 346, 470, 488, 530
Alw26I GTCTC 3 cut(s) 139, 315, 621
AlwNI CAGNNNCTG 1 cut(s) 294
AoxI GGCC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 222
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 1 cut(s) 131
BaeI ACNNNNGTAYC 1 cut(s) 39
BanII GRGCYC 1 cut(s) 36
BauI CACGAG 1 cut(s) 618
BccI CCATC 2 cut(s) 176, 397
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BciT130I CCWGG 1 cut(s) 50
BcoDI GTCTC 3 cut(s) 139, 315, 621
BfaI CTAG 1 cut(s) 29
BglI GCCNNNNNGGC 1 cut(s) 213
BglII AGATCT 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 50
BmeRI GACNNNNNGTC 1 cut(s) 480
BmgT120I GGNCC 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 50
BseGI GGATG 2 cut(s) 187, 441
BseMII CTCAG 1 cut(s) 485
BseRI GAGGAG 1 cut(s) 440
Bsh1285I CGRYCG 2 cut(s) 21, 481
BshFI GGCC 1 cut(s) 207
BsiEI CGRYCG 2 cut(s) 21, 481
BsmAI GTCTC 3 cut(s) 139, 315, 621
BsnI GGCC 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 36
Bsp1407I TGTACA 1 cut(s) 340
Bsp143I GATC 4 cut(s) 18, 190, 367, 478
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 484
BspHI TCATGA 2 cut(s) 226, 319
BsrGI TGTACA 1 cut(s) 340
BssMI GATC 4 cut(s) 18, 190, 367, 478
BssSI CACGAG 1 cut(s) 618
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 83
BstAUI TGTACA 1 cut(s) 340
BstDEI CTNAG 1 cut(s) 471
BstF5I GGATG 2 cut(s) 187, 441
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 4 cut(s) 21, 193, 370, 481
BstMAI GTCTC 3 cut(s) 139, 315, 621
BstMBI GATC 4 cut(s) 18, 190, 367, 478
BstMCI CGRYCG 2 cut(s) 21, 481
BstMWI GCNNNNNNNGC 2 cut(s) 213, 609
BstNI CCWGG 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 48
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 187, 441
BtsIMutI CAGTG 1 cut(s) 548
CaiI CAGNNNCTG 1 cut(s) 294
CciI TCATGA 2 cut(s) 226, 319
CfoI GCGC 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 206
CseI GACGC 1 cut(s) 575
Csp6I GTAC 3 cut(s) 15, 341, 521
CviAII CATG 7 cut(s) 131, 203, 227, 257, 320, 505, 613
CviQI GTAC 3 cut(s) 15, 341, 521
DdeI CTNAG 1 cut(s) 471
DpnI GATC 4 cut(s) 20, 192, 369, 480
DpnII GATC 4 cut(s) 18, 190, 367, 478
DriI GACNNNNNGTC 1 cut(s) 480
Eam1105I GACNNNNNGTC 1 cut(s) 480
Eco24I GRGCYC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 36
FaeI CATG 7 cut(s) 134, 206, 230, 260, 323, 508, 616
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FatI CATG 7 cut(s) 130, 202, 226, 256, 319, 504, 612
FokI GGATG 2 cut(s) 194, 428
FriOI GRGCYC 1 cut(s) 36
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 250
HaeIII GGCC 1 cut(s) 207
HgaI GACGC 1 cut(s) 575
HhaI GCGC 1 cut(s) 251
Hin1II CATG 7 cut(s) 134, 206, 230, 260, 323, 508, 616
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 4 cut(s) 69, 158, 291, 539
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 286, 496, 523
Hpy188I TCNGA 2 cut(s) 157, 474
Hpy188III TCNNGA 4 cut(s) 73, 227, 320, 493
Hpy8I GTNNAC 3 cut(s) 286, 496, 523
Hpy99I CGWCG 2 cut(s) 146, 591
HpyAV CCTTC 2 cut(s) 4, 110
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 2 cut(s) 359, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 213, 609
HpyF3I CTNAG 1 cut(s) 471
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 7 cut(s) 134, 206, 230, 260, 323, 508, 616
HspAI GCGC 1 cut(s) 249
Kzo9I GATC 4 cut(s) 18, 190, 367, 478
LmnI GCTCC 3 cut(s) 42, 238, 406
LpnPI CCDG 4 cut(s) 35, 62, 198, 220
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 1 cut(s) 350
MalI GATC 4 cut(s) 20, 192, 369, 480
MboI GATC 4 cut(s) 18, 190, 367, 478
MboII GAAGA 2 cut(s) 98, 572
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 2 cut(s) 222, 446
MlyI GAGTC 2 cut(s) 152, 548
MnlI CCTC 3 cut(s) 172, 418, 421
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 50
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 213, 609
NdeII GATC 4 cut(s) 18, 190, 367, 478
NlaIII CATG 7 cut(s) 134, 206, 230, 260, 323, 508, 616
NmeAIII GCCGAG 1 cut(s) 588
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 1 cut(s) 616
PagI TCATGA 2 cut(s) 226, 319
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 2 cut(s) 69, 291
Ple19I CGATCG 2 cut(s) 21, 481
PleI GAGTC 2 cut(s) 152, 547
PpsI GAGTC 2 cut(s) 152, 547
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspPI GGNCC 1 cut(s) 206
PstNI CAGNNNCTG 1 cut(s) 294
PsuI RGATCY 1 cut(s) 190
PvuI CGATCG 2 cut(s) 21, 481
RsaI GTAC 3 cut(s) 16, 342, 522
RsaNI GTAC 3 cut(s) 15, 341, 521
SaqAI TTAA 1 cut(s) 174
Sau3AI GATC 4 cut(s) 18, 190, 367, 478
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 2 cut(s) 152, 548
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 1 cut(s) 36
SetI ASST 8 cut(s) 8, 47, 59, 121, 348, 472, 490, 532
Sse9I AATT 2 cut(s) 222, 446
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 1 cut(s) 59
TaqI TCGA 2 cut(s) 336, 537
TaqII GACCGA 1 cut(s) 491
TasI AATT 2 cut(s) 222, 446
TatI WGTACW 2 cut(s) 340, 520
TfiI GAWTC 2 cut(s) 69, 291
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 555
TseFI GTSAC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 4 cut(s) 119, 129, 215, 336
TspRI CASTG 1 cut(s) 555
XapI RAATTY 1 cut(s) 222
XceI RCATGY 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.