Rh5AG504300

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004137
Physical Location & Seq
Forward (+)
85404156 .. 85406566
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG504300.1

Sequence Viewer

Length: 633 bp
ATGCAAATTTCAAATTGTGCTGCTGTTACATTGTTGGCTTTTTTATTGAGCCTACAAGGAGCTTTTGGGATCAGATTTGTGATCGACAGAGATGAATGCTTCTCTCATAATGTTTTGTATGAAGGAGATACTGTCCACCTCTCTTTCGTTGTAATTAAGGTTGATGCATCTTGGCATTATACTCAAGACGGTGTTGATCTCCTGGTGAAGGGGCCTTCTGGTGAGCAGATACATGAGTTTCGTGATAAGACAAGCGAGAAATTCGAGTTTGTCACTCATCACAAAGGAATCCATCAGTTTTGTTTCACCAACAAGTCACCTTATCATGAAACCATAGACTTTGACTTACATGTCAGCCATTTTACATACTATGATCAGCATGCAAAAGATGAGCATTTTAACCCTTTGTTAGATCAGATAACAAAATTAGAGGAAGCTCTTTACAACATTCAGTTTGAGCAGCATTGGCTTGAGGCTCAGACCGAACGCCAGGCAATAGTGAACGACGCAATGAGCCGGAGGGCGATTCACAAGGCCTTTTTTGAATCAGCAGGACTTGTTGGTGCCAGTGTTCTCCAAGTTTACCTTCTTCGCCGCCTCTTTGAACGGAAGCTCGGGATGTCCAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

210

Amino Acids

24.36

Weight (kDa)

5.93

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 31 - 202 1.5e-32 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AccB1I GGYRCC 1 cut(s) 563
AciI CCGC 1 cut(s) 595
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 2 cut(s) 6, 260
AfiI CCNNNNNNNGG 1 cut(s) 208
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 3 cut(s) 12, 545, 605
AjnI CCWGG 2 cut(s) 201, 489
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
AluBI AGCT 3 cut(s) 62, 437, 613
AluI AGCT 3 cut(s) 62, 437, 613
AlwI GGATC 1 cut(s) 77
AlwNI CAGNNNCTG 1 cut(s) 630
Ama87I CYCGRG 1 cut(s) 614
AoxI GGCC 2 cut(s) 212, 534
ApeKI GCWGC 2 cut(s) 20, 460
ApoI RAATTY 2 cut(s) 6, 260
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 4 cut(s) 217, 233, 298, 309
AvaI CYCGRG 1 cut(s) 614
BaeI ACNNNNGTAYC 2 cut(s) 221, 254
BanI GGYRCC 1 cut(s) 563
BbvI GCAGC 2 cut(s) 7, 472
BccI CCATC 1 cut(s) 300
BciT130I CCWGG 2 cut(s) 203, 491
BclI TGATCA 1 cut(s) 373
BisI GCNGC 3 cut(s) 21, 461, 595
BlsI GCNGC 3 cut(s) 22, 462, 596
Bme1390I CCNGG 2 cut(s) 203, 491
BmeT110I CYCGRG 1 cut(s) 614
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 2 cut(s) 213, 565
BmrFI CCNGG 2 cut(s) 203, 491
BmsI GCATC 2 cut(s) 154, 176
BpuEI CTTGAG 2 cut(s) 168, 491
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 567
Bse3DI GCAATG 1 cut(s) 516
BseBI CCWGG 2 cut(s) 203, 491
BseGI GGATG 1 cut(s) 624
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 1 cut(s) 491
BseNI ACTGG 1 cut(s) 567
BseXI GCAGC 2 cut(s) 7, 472
BshFI GGCC 2 cut(s) 214, 536
BshNI GGYRCC 1 cut(s) 563
BsiHKCI CYCGRG 1 cut(s) 614
BsiSI CCGG 1 cut(s) 517
BslI CCNNNNNNNGG 1 cut(s) 208
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 2 cut(s) 214, 536
BsoBI CYCGRG 1 cut(s) 614
Bsp143I GATC 5 cut(s) 69, 81, 196, 373, 412
BspACI CCGC 1 cut(s) 595
BspANI GGCC 2 cut(s) 214, 536
BspCNI CTCAG 1 cut(s) 490
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 2 cut(s) 213, 565
BspPI GGATC 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 516
BsrI ACTGG 1 cut(s) 567
BssMI GATC 5 cut(s) 69, 81, 196, 373, 412
Bst2UI CCWGG 2 cut(s) 203, 491
Bst4CI ACNGT 2 cut(s) 133, 191
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 1 cut(s) 477
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 1 cut(s) 624
BstKTI GATC 5 cut(s) 72, 84, 199, 376, 415
BstMBI GATC 5 cut(s) 69, 81, 196, 373, 412
BstMWI GCNNNNNNNGC 1 cut(s) 466
BstNI CCWGG 2 cut(s) 203, 491
BstNSI RCATGY 2 cut(s) 353, 383
BstSCI CCNGG 2 cut(s) 201, 489
BstV1I GCAGC 2 cut(s) 7, 472
BsuRI GGCC 2 cut(s) 214, 536
BtsCI GGATG 1 cut(s) 624
BtsIMutI CAGTG 1 cut(s) 574
Cac8I GCNNGC 1 cut(s) 381
CaiI CAGNNNCTG 1 cut(s) 630
CciI TCATGA 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 1 cut(s) 515
CviAII CATG 4 cut(s) 233, 326, 350, 380
DdeI CTNAG 1 cut(s) 477
DpnI GATC 5 cut(s) 71, 83, 198, 375, 414
DpnII GATC 5 cut(s) 69, 81, 196, 373, 412
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
Eco147I AGGCCT 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 614
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 201, 489
EcoT22I ATGCAT 1 cut(s) 169
FaeI CATG 4 cut(s) 236, 329, 353, 383
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FatI CATG 4 cut(s) 232, 325, 349, 379
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 3 cut(s) 21, 461, 595
Fsp4HI GCNGC 3 cut(s) 21, 461, 595
GluI GCNGC 3 cut(s) 21, 461, 595
HaeIII GGCC 2 cut(s) 214, 536
HapII CCGG 1 cut(s) 517
HgaI GACGC 1 cut(s) 515
Hin1II CATG 4 cut(s) 236, 329, 353, 383
HinfI GANTC 4 cut(s) 288, 526, 545, 627
HpaII CCGG 1 cut(s) 517
HphI GGTGA 4 cut(s) 217, 233, 298, 309
Hpy166II GTNNAC 3 cut(s) 136, 502, 583
Hpy188I TCNGA 4 cut(s) 74, 417, 480, 632
Hpy188III TCNNGA 5 cut(s) 185, 242, 326, 616, 624
Hpy8I GTNNAC 3 cut(s) 136, 502, 583
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 4 cut(s) 116, 202, 225, 596
HpyCH4III ACNGT 2 cut(s) 133, 191
HpyCH4V TGCA 3 cut(s) 4, 167, 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 466
HpyF3I CTNAG 1 cut(s) 477
Hsp92II CATG 4 cut(s) 236, 329, 353, 383
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 5 cut(s) 69, 81, 196, 373, 412
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 8 cut(s) 188, 204, 215, 476, 503, 530, 537, 580
Lsp1109I GCAGC 2 cut(s) 7, 472
LweI GCATC 2 cut(s) 154, 176
MaeIII GTNAC 3 cut(s) 25, 271, 315
MalI GATC 5 cut(s) 71, 83, 198, 375, 414
MboI GATC 5 cut(s) 69, 81, 196, 373, 412
MboII GAAGA 1 cut(s) 581
MluCI AATT 5 cut(s) 6, 13, 153, 260, 425
MnlI CCTC 5 cut(s) 149, 424, 466, 513, 608
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 2 cut(s) 156, 399
MspI CCGG 1 cut(s) 517
MspR9I CCNGG 2 cut(s) 203, 491
Mva1269I GAATGC 1 cut(s) 101
MvaI CCWGG 2 cut(s) 203, 491
MwoI GCNNNNNNNGC 1 cut(s) 466
NdeII GATC 5 cut(s) 69, 81, 196, 373, 412
NlaIII CATG 4 cut(s) 236, 329, 353, 383
NlaIV GGNNCC 2 cut(s) 213, 565
NmuCI GTSAC 2 cut(s) 271, 315
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 353, 383
PaeI GCATGC 1 cut(s) 383
PagI TCATGA 1 cut(s) 325
PceI AGGCCT 1 cut(s) 536
PciI ACATGT 1 cut(s) 349
PctI GAATGC 1 cut(s) 101
PfeI GAWTC 3 cut(s) 288, 526, 545
PkrI GCNGC 3 cut(s) 22, 462, 596
PscI ACATGT 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 201, 489
PspGI CCWGG 2 cut(s) 201, 489
PspN4I GGNNCC 2 cut(s) 213, 565
PspPI GGNCC 1 cut(s) 212
PstNI CAGNNNCTG 1 cut(s) 630
SaqAI TTAA 2 cut(s) 156, 399
SatI GCNGC 3 cut(s) 21, 461, 595
Sau3AI GATC 5 cut(s) 69, 81, 196, 373, 412
Sau96I GGNCC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 203, 491
SetI ASST 7 cut(s) 64, 141, 162, 322, 439, 588, 615
SfaNI GCATC 2 cut(s) 154, 176
SmlI CTYRAG 2 cut(s) 183, 470
SmoI CTYRAG 2 cut(s) 183, 470
SphI GCATGC 1 cut(s) 383
Sse9I AATT 5 cut(s) 6, 13, 153, 260, 425
SseBI AGGCCT 1 cut(s) 536
SsiI CCGC 1 cut(s) 595
StuI AGGCCT 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 201, 489
TaaI ACNGT 2 cut(s) 133, 191
TaqI TCGA 2 cut(s) 84, 264
TaqII GACCGA 1 cut(s) 497
TasI AATT 5 cut(s) 6, 13, 153, 260, 425
TauI GCSGC 1 cut(s) 597
TfiI GAWTC 3 cut(s) 288, 526, 545
Tru1I TTAA 2 cut(s) 156, 399
Tru9I TTAA 2 cut(s) 156, 399
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 2 cut(s) 271, 315
TseI GCWGC 2 cut(s) 20, 460
Tsp45I GTSAC 2 cut(s) 271, 315
TspDTI ATGAA 3 cut(s) 108, 135, 342
TspGWI ACGGA 1 cut(s) 622
TspRI CASTG 1 cut(s) 574
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 2 cut(s) 6, 260
XceI RCATGY 2 cut(s) 353, 383
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.