RLG00000036636

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004137
Physical Location & Seq
Forward (+)
82793997 .. 82796655
2659 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036636

Sequence Viewer

Length: 711 bp
ATGCAGTCGACGGCGACGGTAGTCAGGGTTGACGGCGGCGACAACCGACCCCTGTTAGTCGGGGTTTTGGCCACGGCGATGCAAATTTCAAATTGTGCTGCTGTTACATTGTTGGCTTTTTTATTGAGCCTACAAGGAGCTTTTGGGATCAGATTTGTGATCGACAGAGATGAATGCTTCTCTCATAATGTTTTGTATGAAGGAGATACTGTCCACCTCTCTTTTGTTGTAATTAAGGTTGATGCATCTTGGCATTATACTCAAGACGGTGTTGATCTCCTGGTGAAGGGGCCTTCTGGTGAGCAGATACATGAGTTTCGTGATAAGACAAGCGAGAAATTCGAGTTTGTCACTCGTCACAAAGGAATCCATCAGTTCTGTTTCACCAACAAGTCACCTTATCATGAAACCATAGACTTTGACTTACATGTCAGCCATTTTACATACTATGATCAGCATGCAAAAGATGAGCATTTTAACCCTTTGTTAGATCAGATAACAAAGTTAGAGGAAGCTCTTTACAACATTCAGTTTGAGCAGCATTGGCTTGAGGCTCAGACCGAACGCCAGGCAATAGTGAACGACGCAATGAGCCGGAGGGCGATTCACAAGGCCTTTTTTGAATCAGCAGGACTTGTTGGTGCCAGTGTTCTCCAAGTTTACCTTCTTCGCCGCCTCTTTGAACGGAAGCTTGGGATGTCCAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

237

Amino Acids

27.01

Weight (kDa)

5.98

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 57 - 228 6.1e-32 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 641
AccI GTMKAC 1 cut(s) 8
AciI CCGC 2 cut(s) 36, 673
AclWI GGATC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 84, 338
AfiI CCNNNNNNNGG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 427
AgsI TTSAA 3 cut(s) 90, 623, 683
AjnI CCWGG 2 cut(s) 279, 567
AjuI GAANNNNNNNTTGG 4 cut(s) 648, 675, 680, 707
AluBI AGCT 3 cut(s) 140, 515, 691
AluI AGCT 3 cut(s) 140, 515, 691
AlwI GGATC 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 708
AoxI GGCC 3 cut(s) 69, 290, 612
ApeKI GCWGC 2 cut(s) 98, 538
ApoI RAATTY 2 cut(s) 84, 338
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 4 cut(s) 295, 311, 376, 387
BaeI ACNNNNGTAYC 2 cut(s) 299, 332
BalI TGGCCA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 641
BbvI GCAGC 2 cut(s) 85, 550
BccI CCATC 1 cut(s) 378
BceAI ACGGC 3 cut(s) 27, 49, 90
BciT130I CCWGG 2 cut(s) 281, 569
BclI TGATCA 1 cut(s) 451
BisI GCNGC 4 cut(s) 37, 99, 539, 673
BlsI GCNGC 4 cut(s) 38, 100, 540, 674
Bme1390I CCNGG 2 cut(s) 281, 569
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 2 cut(s) 291, 643
BmrFI CCNGG 2 cut(s) 281, 569
BmsI GCATC 3 cut(s) 69, 232, 254
BoxI GACNNNNGTC 1 cut(s) 20
BpuEI CTTGAG 2 cut(s) 246, 569
BsaJI CCNNGG 1 cut(s) 72
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse1I ACTGG 1 cut(s) 645
Bse3DI GCAATG 1 cut(s) 594
BseBI CCWGG 2 cut(s) 281, 569
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 1 cut(s) 702
BseLI CCNNNNNNNGG 1 cut(s) 286
BseMI GCAATG 1 cut(s) 594
BseMII CTCAG 1 cut(s) 569
BseNI ACTGG 1 cut(s) 645
BseXI GCAGC 2 cut(s) 85, 550
BshFI GGCC 3 cut(s) 71, 292, 614
BshNI GGYRCC 1 cut(s) 641
BsiSI CCGG 1 cut(s) 595
BslI CCNNNNNNNGG 1 cut(s) 286
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 3 cut(s) 71, 292, 614
Bsp143I GATC 5 cut(s) 147, 159, 274, 451, 490
BspACI CCGC 2 cut(s) 36, 673
BspANI GGCC 3 cut(s) 71, 292, 614
BspCNI CTCAG 1 cut(s) 568
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 291, 643
BspPI GGATC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 1 cut(s) 645
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 5 cut(s) 147, 159, 274, 451, 490
Bst2UI CCWGG 2 cut(s) 281, 569
Bst4CI ACNGT 3 cut(s) 19, 211, 269
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 1 cut(s) 555
BstDSI CCRYGG 1 cut(s) 72
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 1 cut(s) 702
BstKTI GATC 5 cut(s) 150, 162, 277, 454, 493
BstMBI GATC 5 cut(s) 147, 159, 274, 451, 490
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 2 cut(s) 281, 569
BstNSI RCATGY 2 cut(s) 431, 461
BstPAI GACNNNNGTC 1 cut(s) 20
BstSCI CCNGG 2 cut(s) 279, 567
BstV1I GCAGC 2 cut(s) 85, 550
BsuRI GGCC 3 cut(s) 71, 292, 614
BtgI CCRYGG 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 92
BtsCI GGATG 1 cut(s) 702
BtsIMutI CAGTG 1 cut(s) 652
Cac8I GCNNGC 1 cut(s) 459
CaiI CAGNNNCTG 1 cut(s) 708
CciI TCATGA 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 290
CseI GACGC 1 cut(s) 593
CviAII CATG 4 cut(s) 311, 404, 428, 458
DdeI CTNAG 1 cut(s) 555
DpnI GATC 5 cut(s) 149, 161, 276, 453, 492
DpnII GATC 5 cut(s) 147, 159, 274, 451, 490
DrdI GACNNNNNNGTC 1 cut(s) 428
DseDI GACNNNNNNGTC 1 cut(s) 428
EaeI YGGCCR 1 cut(s) 69
Eco147I AGGCCT 1 cut(s) 614
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 290
EcoRII CCWGG 2 cut(s) 279, 567
EcoT22I ATGCAT 1 cut(s) 247
FaeI CATG 4 cut(s) 314, 407, 431, 461
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FatI CATG 4 cut(s) 310, 403, 427, 457
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 4 cut(s) 37, 99, 539, 673
Fsp4HI GCNGC 4 cut(s) 37, 99, 539, 673
GluI GCNGC 4 cut(s) 37, 99, 539, 673
HaeIII GGCC 3 cut(s) 71, 292, 614
HapII CCGG 1 cut(s) 595
HgaI GACGC 1 cut(s) 593
Hin1II CATG 4 cut(s) 314, 407, 431, 461
HincII GTYRAC 2 cut(s) 9, 31
HindII GTYRAC 2 cut(s) 9, 31
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 4 cut(s) 366, 604, 623, 705
HpaII CCGG 1 cut(s) 595
HphI GGTGA 4 cut(s) 295, 311, 376, 387
Hpy166II GTNNAC 5 cut(s) 9, 31, 214, 580, 661
Hpy188I TCNGA 4 cut(s) 152, 495, 558, 710
Hpy188III TCNNGA 4 cut(s) 263, 320, 404, 702
Hpy8I GTNNAC 5 cut(s) 9, 31, 214, 580, 661
Hpy99I CGWCG 3 cut(s) 13, 19, 587
HpyAV CCTTC 4 cut(s) 194, 280, 303, 674
HpyCH4III ACNGT 3 cut(s) 19, 211, 269
HpyCH4V TGCA 4 cut(s) 4, 82, 245, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 1 cut(s) 555
Hsp92II CATG 4 cut(s) 314, 407, 431, 461
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 5 cut(s) 147, 159, 274, 451, 490
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 2 cut(s) 85, 550
LweI GCATC 3 cut(s) 69, 232, 254
MaeIII GTNAC 4 cut(s) 103, 349, 356, 393
MalI GATC 5 cut(s) 149, 161, 276, 453, 492
MboI GATC 5 cut(s) 147, 159, 274, 451, 490
MboII GAAGA 1 cut(s) 659
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 4 cut(s) 84, 91, 231, 338
MluNI TGGCCA 1 cut(s) 71
MnlI CCTC 5 cut(s) 227, 502, 544, 591, 686
Mox20I TGGCCA 1 cut(s) 71
Mph1103I ATGCAT 1 cut(s) 247
MscI TGGCCA 1 cut(s) 71
MseI TTAA 2 cut(s) 234, 477
MslI CAYNNNNRTG 1 cut(s) 77
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 1 cut(s) 595
MspR9I CCNGG 2 cut(s) 281, 569
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 2 cut(s) 281, 569
MwoI GCNNNNNNNGC 1 cut(s) 544
NdeII GATC 5 cut(s) 147, 159, 274, 451, 490
NlaIII CATG 4 cut(s) 314, 407, 431, 461
NlaIV GGNNCC 2 cut(s) 291, 643
NmuCI GTSAC 3 cut(s) 349, 356, 393
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 2 cut(s) 431, 461
PaeI GCATGC 1 cut(s) 461
PagI TCATGA 1 cut(s) 403
PceI AGGCCT 1 cut(s) 614
PciI ACATGT 1 cut(s) 427
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 3 cut(s) 366, 604, 623
PkrI GCNGC 4 cut(s) 38, 100, 540, 674
PscI ACATGT 1 cut(s) 427
PshAI GACNNNNGTC 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 279, 567
PspGI CCWGG 2 cut(s) 279, 567
PspN4I GGNNCC 2 cut(s) 291, 643
PspPI GGNCC 1 cut(s) 290
PstNI CAGNNNCTG 1 cut(s) 708
RseI CAYNNNNRTG 1 cut(s) 77
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 2 cut(s) 234, 477
SatI GCNGC 4 cut(s) 37, 99, 539, 673
Sau3AI GATC 5 cut(s) 147, 159, 274, 451, 490
Sau96I GGNCC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 281, 569
SetI ASST 7 cut(s) 142, 219, 240, 400, 517, 666, 693
SfaNI GCATC 3 cut(s) 69, 232, 254
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 261, 548
SmoI CTYRAG 2 cut(s) 261, 548
SphI GCATGC 1 cut(s) 461
Sse9I AATT 4 cut(s) 84, 91, 231, 338
SseBI AGGCCT 1 cut(s) 614
SsiI CCGC 2 cut(s) 36, 673
StuI AGGCCT 1 cut(s) 614
StyD4I CCNGG 2 cut(s) 279, 567
TaaI ACNGT 3 cut(s) 19, 211, 269
TaqI TCGA 3 cut(s) 8, 162, 342
TaqII GACCGA 1 cut(s) 575
TasI AATT 4 cut(s) 84, 91, 231, 338
TauI GCSGC 2 cut(s) 39, 675
TfiI GAWTC 3 cut(s) 366, 604, 623
Tru1I TTAA 2 cut(s) 234, 477
Tru9I TTAA 2 cut(s) 234, 477
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 3 cut(s) 349, 356, 393
TseI GCWGC 2 cut(s) 98, 538
Tsp45I GTSAC 3 cut(s) 349, 356, 393
TspDTI ATGAA 3 cut(s) 186, 213, 420
TspGWI ACGGA 1 cut(s) 700
TspRI CASTG 1 cut(s) 652
XagI CCTNNNNNAGG 1 cut(s) 284
XapI RAATTY 2 cut(s) 84, 338
XceI RCATGY 2 cut(s) 431, 461
XmiI GTMKAC 1 cut(s) 8
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.