RLG00000020865

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004137
Physical Location & Seq
Forward (+)
69662044 .. 69665131
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020865

Sequence Viewer

Length: 720 bp
ATGGATCAACATATCCAATGTCAAACCCATTGTCAATTACACCGTACCCTTTTTGTTTCTCTCTCCAATTTTGATTGTTTCACAGAGAAGATGGAGCTGCTGGGATTGTTAATGATGGGTTTTGTGGTCTTGTGCCAAATGAAAGGCTCCGTTGGGTTGAGGTTCGTGATAGATAGAGAAGAGTGCTTCTCTCATGAGGCAAAGTATGAGGGTGACACTGTTCATGTCTCTTTTGTTGTGATCAAAATCGATTCGACTTGGCATTACACTCAAGAAGGAGTTGATCTTGTGGTAAAGGGGCCTTCTGGTGAACAACTTCATGACATACGTGACAAGACGAGTGAGAAGTTTGAATTTGTGGTTCAGAAGGCCGGTGTTCATCACTTCTGCTTCACTAACAAGTCTCCTTACCATGAAACCATTGATTTTGATGTACATTCTGCGCACTTCACATACTATGACGAGCATGCTAAAGATGAGCATTTCAAACCCTTGTTTGAACAGATTCAAAAGTTGGAGGAAGCCATTTACAATATCCAATTTGAACAACACTGGCTAGAGGCTGAGACCGACCGCCAGGCAATATTGAATGAAGGAATGAGTCGGAGAGCAACTCACAAGGCAATGTTTGAATCAGCTGCACTTATTGGAACCAGCCTCCTCCAAGTTTTTCTTTTGAAACGTTTGTTTGAGCGCAAGCTTGGGTCATCTAGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

240

Amino Acids

27.87

Weight (kDa)

5.86

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 59 - 231 1.7e-33 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 444
AciI CCGC 1 cut(s) 574
AclI AACGTT 1 cut(s) 682
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 353
AfaI GTAC 2 cut(s) 46, 435
AgsI TTSAA 8 cut(s) 353, 487, 500, 509, 545, 589, 632, 679
AjnI CCWGG 1 cut(s) 576
AluBI AGCT 3 cut(s) 97, 638, 700
AluI AGCT 3 cut(s) 97, 638, 700
Alw26I GTCTC 3 cut(s) 232, 408, 560
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 299, 369
ApeKI GCWGC 2 cut(s) 97, 638
ApoI RAATTY 1 cut(s) 353
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
Asp700I GAANNNNTTC 2 cut(s) 315, 504
AspLEI GCGC 2 cut(s) 445, 696
AspS9I GGNCC 1 cut(s) 299
AsuHPI GGTGA 2 cut(s) 224, 320
BbvI GCAGC 2 cut(s) 84, 625
BccI CCATC 2 cut(s) 85, 109
BciT130I CCWGG 1 cut(s) 578
BclI TGATCA 1 cut(s) 240
BcoDI GTCTC 3 cut(s) 232, 408, 560
BfaI CTAG 2 cut(s) 557, 711
BisI GCNGC 2 cut(s) 98, 639
BlsI GCNGC 2 cut(s) 99, 640
Bme1390I CCNGG 1 cut(s) 578
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 3 cut(s) 148, 300, 652
BmrFI CCNGG 1 cut(s) 578
BplI GAGNNNNNCTC 4 cut(s) 173, 205, 598, 630
BpuEI CTTGAG 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 249
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 245
BsaI GGTCTC 1 cut(s) 560
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 557
Bse3DI GCAATG 1 cut(s) 630
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 1 cut(s) 578
BseCI ATCGAT 1 cut(s) 249
BseJI GATNNNNATC 1 cut(s) 245
BseMI GCAATG 1 cut(s) 630
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 1 cut(s) 650
BseXI GCAGC 2 cut(s) 84, 625
BseYI CCCAGC 1 cut(s) 100
BsgI GTGCAG 1 cut(s) 624
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 2 cut(s) 301, 371
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 574
BsiSI CCGG 1 cut(s) 372
BsmAI GTCTC 3 cut(s) 232, 408, 560
BsnI GGCC 2 cut(s) 301, 371
Bso31I GGTCTC 1 cut(s) 560
Bsp1407I TGTACA 1 cut(s) 433
Bsp143I GATC 3 cut(s) 4, 240, 283
BspACI CCGC 1 cut(s) 574
BspANI GGCC 2 cut(s) 301, 371
BspCNI CTCAG 1 cut(s) 556
BspDI ATCGAT 1 cut(s) 249
BspHI TCATGA 2 cut(s) 193, 319
BspLI GGNNCC 3 cut(s) 148, 300, 652
BspPI GGATC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 371
BssMI GATC 3 cut(s) 4, 240, 283
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 44, 220
Bst6I CTCTTC 1 cut(s) 174
BstAUI TGTACA 1 cut(s) 433
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 468, 698
BstDEI CTNAG 1 cut(s) 564
BstHHI GCGC 2 cut(s) 445, 696
BstKTI GATC 3 cut(s) 7, 243, 286
BstMAI GTCTC 3 cut(s) 232, 408, 560
BstMBI GATC 3 cut(s) 4, 240, 283
BstMCI CGRYCG 1 cut(s) 574
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 576
BstV1I GCAGC 2 cut(s) 84, 625
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 2 cut(s) 301, 371
BsuTUI ATCGAT 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 216, 550
Cac8I GCNNGC 2 cut(s) 468, 698
CciI TCATGA 2 cut(s) 193, 319
CfoI GCGC 2 cut(s) 445, 696
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 1 cut(s) 299
ClaI ATCGAT 1 cut(s) 249
Csp6I GTAC 2 cut(s) 45, 434
CviAII CATG 5 cut(s) 194, 224, 320, 413, 467
CviQI GTAC 2 cut(s) 45, 434
DdeI CTNAG 1 cut(s) 564
DpnI GATC 3 cut(s) 6, 242, 285
DpnII GATC 3 cut(s) 4, 240, 283
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 576
FaeI CATG 5 cut(s) 197, 227, 323, 416, 470
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FatI CATG 5 cut(s) 193, 223, 319, 412, 466
FbaI TGATCA 1 cut(s) 240
Fnu4HI GCNGC 2 cut(s) 98, 639
Fsp4HI GCNGC 2 cut(s) 98, 639
FspBI CTAG 2 cut(s) 557, 711
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 2 cut(s) 444, 695
GluI GCNGC 2 cut(s) 98, 639
GsaI CCCAGC 1 cut(s) 104
HaeIII GGCC 2 cut(s) 301, 371
HapII CCGG 1 cut(s) 372
HhaI GCGC 2 cut(s) 445, 696
Hin1II CATG 5 cut(s) 197, 227, 323, 416, 470
Hin6I GCGC 2 cut(s) 443, 694
HinP1I GCGC 2 cut(s) 443, 694
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 5 cut(s) 251, 505, 601, 632, 714
HpaII CCGG 1 cut(s) 372
HphI GGTGA 2 cut(s) 224, 320
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 2 cut(s) 366, 606
Hpy188III TCNNGA 5 cut(s) 166, 194, 272, 320, 711
Hpy8I GTNNAC 1 cut(s) 311
HpyAV CCTTC 4 cut(s) 269, 312, 361, 587
HpyCH4III ACNGT 2 cut(s) 44, 220
HpyCH4IV ACGT 2 cut(s) 328, 682
HpyCH4V TGCA 1 cut(s) 641
HpyF3I CTNAG 1 cut(s) 564
HpySE526I ACGT 2 cut(s) 328, 682
Hsp92II CATG 5 cut(s) 197, 227, 323, 416, 470
HspAI GCGC 2 cut(s) 443, 694
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 3 cut(s) 4, 240, 283
LmnI GCTCC 2 cut(s) 94, 152
LpnPI CCDG 7 cut(s) 86, 291, 385, 538, 563, 590, 667
Lsp1109I GCAGC 2 cut(s) 84, 625
MaeI CTAG 2 cut(s) 557, 711
MaeII ACGT 2 cut(s) 328, 682
MaeIII GTNAC 2 cut(s) 212, 329
MalI GATC 3 cut(s) 6, 242, 285
MboI GATC 3 cut(s) 4, 240, 283
MboII GAAGA 2 cut(s) 100, 191
MluCI AATT 4 cut(s) 35, 67, 353, 539
MlyI GAGTC 1 cut(s) 610
MmeI TCCRAC 2 cut(s) 495, 584
MnlI CCTC 7 cut(s) 153, 190, 202, 511, 553, 668, 671
MroXI GAANNNNTTC 2 cut(s) 315, 504
MseI TTAA 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 1 cut(s) 578
MvaI CCWGG 1 cut(s) 578
NdeII GATC 3 cut(s) 4, 240, 283
NlaIII CATG 5 cut(s) 197, 227, 323, 416, 470
NlaIV GGNNCC 3 cut(s) 148, 300, 652
NmuCI GTSAC 2 cut(s) 212, 329
NsbI TGCGCA 1 cut(s) 444
NspI RCATGY 1 cut(s) 470
PaeI GCATGC 1 cut(s) 470
PagI TCATGA 2 cut(s) 193, 319
PdmI GAANNNNTTC 2 cut(s) 315, 504
PfeI GAWTC 3 cut(s) 251, 505, 632
PkrI GCNGC 2 cut(s) 99, 640
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
Ppu21I YACGTR 1 cut(s) 329
Psp1406I AACGTT 1 cut(s) 682
Psp6I CCWGG 1 cut(s) 576
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 3 cut(s) 148, 300, 652
PspPI GGNCC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 2 cut(s) 46, 435
RsaNI GTAC 2 cut(s) 45, 434
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 2 cut(s) 98, 639
Sau3AI GATC 3 cut(s) 4, 240, 283
Sau96I GGNCC 1 cut(s) 299
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 1 cut(s) 578
SetI ASST 6 cut(s) 99, 164, 331, 640, 685, 702
SmlI CTYRAG 1 cut(s) 270
SmoI CTYRAG 1 cut(s) 270
SphI GCATGC 1 cut(s) 470
Sse9I AATT 4 cut(s) 35, 67, 353, 539
SsiI CCGC 1 cut(s) 574
SspI AATATT 1 cut(s) 585
SspMI CTAG 2 cut(s) 557, 711
StyD4I CCNGG 1 cut(s) 576
TaaI ACNGT 2 cut(s) 44, 220
TaiI ACGT 2 cut(s) 331, 685
TaqI TCGA 2 cut(s) 249, 254
TaqII GACCGA 1 cut(s) 584
TasI AATT 4 cut(s) 35, 67, 353, 539
TatI WGTACW 1 cut(s) 433
TfiI GAWTC 3 cut(s) 251, 505, 632
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 2 cut(s) 223, 557
TseFI GTSAC 2 cut(s) 212, 329
TseI GCWGC 2 cut(s) 97, 638
Tsp45I GTSAC 2 cut(s) 212, 329
TspDTI ATGAA 6 cut(s) 155, 212, 308, 368, 429, 606
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 2 cut(s) 223, 557
XapI RAATTY 1 cut(s) 353
XbaI TCTAGA 1 cut(s) 710
XceI RCATGY 1 cut(s) 470
XmnI GAANNNNTTC 2 cut(s) 315, 504
XspI CTAG 2 cut(s) 557, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.