FvH4_3g42910

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004137
Physical Location & Seq
Forward (+)
35847617 .. 35849888
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42910.t3

Sequence Viewer

Length: 639 bp
ATGCAAATTGGGTTTTCAAGCTGCGCTGCTGTTATATTGTTGGCATTCTTGTTGAGCCTGCAAGGAGCTTTCAGCATCAGATTTGTGATTGACAGAGACGAATGCTTCTCTCATAATGTTTTGTATGAAGGAGATACTGTCCATCTCTCTTTTGTTGTAATTAAGGTTGATGCATCTTGGCATTATACTCAAGACGGTGTTGATCTCATGGTGAAGGGACCTTCTGGTGAGCAGATACATGAGTTTCGTGATAAGACAAGCGAGAAATATGAGTTTGTCACTCGTCACAAAGGAATCCATCAGTTCTGCTTCACCAACAAGTCACCTTATCATGAAACAATAGACTTTGACATACATGTCAGCCATTTTACATACTATGATCAGCATGCAAAAGATGAGCATTTTAACCCTTTGTTAGAGCAGATATCAAAGTTGGAGGAAGCTCTTTACAACATTCAGTTTGAGCAGCATTGGCTTGAGGCTCAGACTGAACGTCAGGCAATAGTGAACGAAGCAATGAGCCGGAGGGCGATTCACAAGGCCTTTTTTGAATCAGCAGGACTTGTTGGTGCTAGTGTTCTCCAAGTTTACCTTCTTCGCCGCCTCTTTGAACGGAAGCTTGGGATGTCCAGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

213

Amino Acids

24.65

Weight (kDa)

5.98

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 32 - 133 5.9e-15 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 356
AciI CCGC 1 cut(s) 601
AflIII ACRYGT 1 cut(s) 355
AgsI TTSAA 3 cut(s) 18, 551, 611
AhdI GACNNNNNGTC 1 cut(s) 492
AjuI GAANNNNNNNTTGG 4 cut(s) 576, 603, 608, 635
AluBI AGCT 4 cut(s) 21, 68, 443, 619
AluI AGCT 4 cut(s) 21, 68, 443, 619
Alw26I GTCTC 1 cut(s) 90
AoxI GGCC 1 cut(s) 540
ApeKI GCWGC 3 cut(s) 21, 26, 466
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 4 cut(s) 223, 239, 304, 315
AvaII GGWCC 1 cut(s) 218
BaeI ACNNNNGTAYC 2 cut(s) 227, 260
BbvI GCAGC 3 cut(s) 8, 13, 478
BccI CCATC 2 cut(s) 150, 306
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 573
BisI GCNGC 4 cut(s) 22, 27, 467, 601
BlsI GCNGC 4 cut(s) 23, 28, 468, 602
Bme18I GGWCC 1 cut(s) 218
BmeRI GACNNNNNGTC 1 cut(s) 492
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 219
BmsI GCATC 3 cut(s) 84, 160, 182
BpuEI CTTGAG 2 cut(s) 174, 497
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bse3DI GCAATG 1 cut(s) 522
BseGI GGATG 1 cut(s) 630
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 1 cut(s) 497
BseXI GCAGC 3 cut(s) 8, 13, 478
BshFI GGCC 1 cut(s) 542
BsiSI CCGG 1 cut(s) 523
BslFI GGGAC 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 90
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 231
BsmI GAATGC 2 cut(s) 44, 107
BsnI GGCC 1 cut(s) 542
Bsp143I GATC 2 cut(s) 202, 379
BspACI CCGC 1 cut(s) 601
BspANI GGCC 1 cut(s) 542
BspCNI CTCAG 1 cut(s) 496
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 522
BssMI GATC 2 cut(s) 202, 379
Bst4CI ACNGT 2 cut(s) 139, 197
BstC8I GCNNGC 2 cut(s) 59, 387
BstDEI CTNAG 1 cut(s) 483
BstF5I GGATG 1 cut(s) 630
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 2 cut(s) 205, 382
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 2 cut(s) 202, 379
BstMWI GCNNNNNNNGC 1 cut(s) 472
BstNSI RCATGY 2 cut(s) 359, 389
BstV1I GCAGC 3 cut(s) 8, 13, 478
BsuRI GGCC 1 cut(s) 542
BtsCI GGATG 1 cut(s) 630
Cac8I GCNNGC 2 cut(s) 59, 387
CciI TCATGA 1 cut(s) 331
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 218
CviAII CATG 5 cut(s) 208, 239, 332, 356, 386
DdeI CTNAG 1 cut(s) 483
DpnI GATC 2 cut(s) 204, 381
DpnII GATC 2 cut(s) 202, 379
DrdI GACNNNNNNGTC 1 cut(s) 356
DriI GACNNNNNGTC 1 cut(s) 492
DseDI GACNNNNNNGTC 1 cut(s) 356
Eam1105I GACNNNNNGTC 1 cut(s) 492
Eco147I AGGCCT 1 cut(s) 542
Eco32I GATATC 1 cut(s) 426
Eco47I GGWCC 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 218
EcoRV GATATC 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 5 cut(s) 211, 242, 335, 359, 389
FalI AAGNNNNNCTT 2 cut(s) 576, 608
FaqI GGGAC 1 cut(s) 231
FatI CATG 5 cut(s) 207, 238, 331, 355, 385
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 4 cut(s) 22, 27, 467, 601
Fsp4HI GCNGC 4 cut(s) 22, 27, 467, 601
FspBI CTAG 1 cut(s) 573
GlaI GCGC 1 cut(s) 25
GluI GCNGC 4 cut(s) 22, 27, 467, 601
HaeIII GGCC 1 cut(s) 542
HapII CCGG 1 cut(s) 523
HhaI GCGC 1 cut(s) 26
Hin1II CATG 5 cut(s) 211, 242, 335, 359, 389
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 3 cut(s) 294, 532, 551
HpaII CCGG 1 cut(s) 523
HphI GGTGA 4 cut(s) 223, 239, 304, 315
Hpy166II GTNNAC 2 cut(s) 508, 589
Hpy188I TCNGA 2 cut(s) 80, 486
Hpy188III TCNNGA 4 cut(s) 191, 248, 332, 630
Hpy8I GTNNAC 2 cut(s) 508, 589
HpyAV CCTTC 4 cut(s) 122, 208, 231, 602
HpyCH4III ACNGT 2 cut(s) 139, 197
HpyCH4IV ACGT 1 cut(s) 493
HpyCH4V TGCA 4 cut(s) 4, 61, 173, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 472
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 1 cut(s) 493
Hsp92II CATG 5 cut(s) 211, 242, 335, 359, 389
HspAI GCGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 2 cut(s) 202, 379
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 5 cut(s) 71, 210, 482, 536, 543
Lsp1109I GCAGC 3 cut(s) 8, 13, 478
LweI GCATC 3 cut(s) 84, 160, 182
MaeI CTAG 1 cut(s) 573
MaeII ACGT 1 cut(s) 493
MaeIII GTNAC 3 cut(s) 277, 284, 321
MalI GATC 2 cut(s) 204, 381
MboI GATC 2 cut(s) 202, 379
MboII GAAGA 1 cut(s) 587
MluCI AATT 2 cut(s) 6, 159
MmeI TCCRAC 1 cut(s) 414
MnlI CCTC 4 cut(s) 430, 472, 519, 614
Mph1103I ATGCAT 1 cut(s) 175
MseI TTAA 2 cut(s) 162, 405
MspI CCGG 1 cut(s) 523
Mva1269I GAATGC 2 cut(s) 44, 107
MwoI GCNNNNNNNGC 1 cut(s) 472
NdeII GATC 2 cut(s) 202, 379
NlaIII CATG 5 cut(s) 211, 242, 335, 359, 389
NlaIV GGNNCC 1 cut(s) 219
NmuCI GTSAC 3 cut(s) 277, 284, 321
NsiI ATGCAT 1 cut(s) 175
NspI RCATGY 2 cut(s) 359, 389
PaeI GCATGC 1 cut(s) 389
PagI TCATGA 1 cut(s) 331
PceI AGGCCT 1 cut(s) 542
PciI ACATGT 1 cut(s) 355
PctI GAATGC 2 cut(s) 44, 107
PfeI GAWTC 3 cut(s) 294, 532, 551
PkrI GCNGC 4 cut(s) 23, 28, 468, 602
PpuMI RGGWCCY 1 cut(s) 218
PscI ACATGT 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 218
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 218
PspPPI RGGWCCY 1 cut(s) 218
SaqAI TTAA 2 cut(s) 162, 405
SatI GCNGC 4 cut(s) 22, 27, 467, 601
Sau3AI GATC 2 cut(s) 202, 379
Sau96I GGNCC 1 cut(s) 218
SetI ASST 9 cut(s) 23, 70, 168, 223, 328, 445, 496, 594, 621
SfaNI GCATC 3 cut(s) 84, 160, 182
SinI GGWCC 1 cut(s) 218
SmlI CTYRAG 2 cut(s) 189, 476
SmoI CTYRAG 2 cut(s) 189, 476
SphI GCATGC 1 cut(s) 389
Sse9I AATT 2 cut(s) 6, 159
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 1 cut(s) 601
SspMI CTAG 1 cut(s) 573
StuI AGGCCT 1 cut(s) 542
TaaI ACNGT 2 cut(s) 139, 197
TaiI ACGT 1 cut(s) 496
TasI AATT 2 cut(s) 6, 159
TauI GCSGC 1 cut(s) 603
TfiI GAWTC 3 cut(s) 294, 532, 551
Tru1I TTAA 2 cut(s) 162, 405
Tru9I TTAA 2 cut(s) 162, 405
TseFI GTSAC 3 cut(s) 277, 284, 321
TseI GCWGC 3 cut(s) 21, 26, 466
Tsp45I GTSAC 3 cut(s) 277, 284, 321
TspDTI ATGAA 2 cut(s) 141, 348
TspGWI ACGGA 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 218
XceI RCATGY 2 cut(s) 359, 389
XspI CTAG 1 cut(s) 573
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.