MD17G1118000.v1.1

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004137
Physical Location & Seq
Reverse (-)
10243610 .. 10246059
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1118000.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGGAGACACGATTCACGTCTCTTTCGTGGTAATCAAAGTCGATTCGACCTGGCATTACACCGACGACGGCGTTGATCTCGTGATAAAGGGACCTGGTGGTGAACAAATTCATGACATACGCGACAAGACAAGCGTGAAGTATGACTTTGTGGTTGAGAAGCAAGGTGTTCACCAGTTCTGCTTCACTAACAAGTCTCCTTACCACGAAACTATTGACTTTGATGTACATGCTGCTCACTTTACATACTATGACCAGCATGCTAAAGACGAGCATTTCACACCCTTGCTGGATCAGATACAAAAGTTGGAGGAAGCCCTCTACAACATCCAATTCGAACAACATTGGTTGGAAGCTGAGACTGACCGCCAGGCAATAGTGAATGAAGGAATGAGCCGTAGAGCAACGCACAAGGCATTGATAGAATCAGCAGCACTTATTGGAGCTAGCTGCCTCCAGGTTTTTCTTTTGAAACGTTTGTTTGAACGCAAGCTTGGATCCTCTAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

171

Amino Acids

19.59

Weight (kDa)

5.71

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 2 - 162 5.2e-29 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 123
AciI CCGC 1 cut(s) 367
AclI AACGTT 1 cut(s) 475
AclWI GGATC 3 cut(s) 300, 492, 505
AcsI RAATTY 1 cut(s) 108
AfaI GTAC 1 cut(s) 228
AgsI TTSAA 2 cut(s) 472, 485
AjiI CACGTC 1 cut(s) 19
AjnI CCWGG 4 cut(s) 50, 94, 369, 456
AjuI GAANNNNNNNTTGG 2 cut(s) 477, 509
AluBI AGCT 4 cut(s) 356, 446, 450, 493
AluI AGCT 4 cut(s) 356, 446, 450, 493
Alw26I GTCTC 3 cut(s) 25, 201, 353
AlwI GGATC 3 cut(s) 300, 492, 505
ApeKI GCWGC 3 cut(s) 233, 431, 450
ApoI RAATTY 1 cut(s) 108
Asp700I GAANNNNTTC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 113, 164
AsuII TTCGAA 1 cut(s) 336
AsuNHI GCTAGC 1 cut(s) 446
AvaII GGWCC 1 cut(s) 92
BaeI ACNNNNGTAYC 2 cut(s) 290, 323
BamHI GGATCC 1 cut(s) 497
BauI CACGAG 1 cut(s) 80
BbvI GCAGC 3 cut(s) 220, 437, 443
BceAI ACGGC 2 cut(s) 85, 381
BciT130I CCWGG 4 cut(s) 52, 96, 371, 458
BcoDI GTCTC 3 cut(s) 25, 201, 353
BfaI CTAG 2 cut(s) 447, 504
BisI GCNGC 3 cut(s) 234, 432, 451
BlsI GCNGC 3 cut(s) 235, 433, 452
Bme1390I CCNGG 4 cut(s) 52, 96, 371, 458
Bme18I GGWCC 1 cut(s) 92
BmgBI CACGTC 1 cut(s) 19
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 2 cut(s) 93, 499
BmrFI CCNGG 4 cut(s) 52, 96, 371, 458
BmtI GCTAGC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 440
Bpu14I TTCGAA 1 cut(s) 336
Bse1I ACTGG 1 cut(s) 175
BseBI CCWGG 4 cut(s) 52, 96, 371, 458
BseGI GGATG 1 cut(s) 327
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 3 cut(s) 220, 437, 443
Bsh1236I CGCG 1 cut(s) 123
BslFI GGGAC 1 cut(s) 105
BsmAI GTCTC 3 cut(s) 25, 201, 353
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 105
Bsp119I TTCGAA 1 cut(s) 336
Bsp1407I TGTACA 1 cut(s) 226
Bsp143I GATC 3 cut(s) 76, 292, 497
BspACI CCGC 1 cut(s) 367
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 1 cut(s) 123
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 93, 499
BspOI GCTAGC 1 cut(s) 450
BspPI GGATC 3 cut(s) 300, 492, 505
BspT104I TTCGAA 1 cut(s) 336
BsrGI TGTACA 1 cut(s) 226
BsrI ACTGG 1 cut(s) 175
BssMI GATC 3 cut(s) 76, 292, 497
BssSI CACGAG 1 cut(s) 80
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 4 cut(s) 52, 96, 371, 458
BstAUI TGTACA 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 261, 448, 491
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 327
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 3 cut(s) 79, 295, 500
BstMAI GTCTC 3 cut(s) 25, 201, 353
BstMBI GATC 3 cut(s) 76, 292, 497
BstNI CCWGG 4 cut(s) 52, 96, 371, 458
BstNSI RCATGY 2 cut(s) 233, 263
BstSCI CCNGG 4 cut(s) 50, 94, 369, 456
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 3 cut(s) 220, 437, 443
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
BtrI CACGTC 1 cut(s) 19
BtsCI GGATG 1 cut(s) 327
Cac8I GCNNGC 3 cut(s) 261, 448, 491
CciI TCATGA 1 cut(s) 112
Cfr13I GGNCC 1 cut(s) 92
CsiI ACCWGGT 1 cut(s) 94
Csp6I GTAC 1 cut(s) 227
CviAII CATG 3 cut(s) 113, 230, 260
CviJI RGCY 6 cut(s) 317, 356, 396, 446, 450, 493
CviKI_1 RGCY 6 cut(s) 317, 356, 396, 446, 450, 493
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 1 cut(s) 357
DpnI GATC 3 cut(s) 78, 294, 499
DpnII GATC 3 cut(s) 76, 292, 497
Eco47I GGWCC 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 4 cut(s) 50, 94, 369, 456
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 3 cut(s) 116, 233, 263
FaiI YATR 7 cut(s) 114, 119, 144, 231, 247, 252, 261
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 1 cut(s) 105
FatI CATG 3 cut(s) 112, 229, 259
Fnu4HI GCNGC 3 cut(s) 234, 432, 451
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 3 cut(s) 234, 432, 451
FspBI CTAG 2 cut(s) 447, 504
GluI GCNGC 3 cut(s) 234, 432, 451
GsuI CTGGAG 1 cut(s) 440
Hin1II CATG 3 cut(s) 116, 233, 263
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 3 cut(s) 13, 44, 425
HphI GGTGA 2 cut(s) 113, 164
Hpy166II GTNNAC 2 cut(s) 104, 172
Hpy188I TCNGA 1 cut(s) 297
Hpy188III TCNNGA 3 cut(s) 82, 113, 504
Hpy8I GTNNAC 2 cut(s) 104, 172
Hpy99I CGWCG 2 cut(s) 68, 71
HpyAV CCTTC 1 cut(s) 380
HpyCH4IV ACGT 2 cut(s) 18, 475
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 2 cut(s) 18, 475
Hsp92II CATG 3 cut(s) 116, 233, 263
Kzo9I GATC 3 cut(s) 76, 292, 497
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 3 cut(s) 220, 437, 443
MabI ACCWGGT 1 cut(s) 94
MaeI CTAG 2 cut(s) 447, 504
MaeII ACGT 2 cut(s) 18, 475
MalI GATC 3 cut(s) 78, 294, 499
MboI GATC 3 cut(s) 76, 292, 497
MflI RGATCY 1 cut(s) 497
MluCI AATT 2 cut(s) 108, 332
MmeI TCCRAC 2 cut(s) 288, 330
MnlI CCTC 4 cut(s) 304, 329, 464, 511
MroXI GAANNNNTTC 1 cut(s) 108
MspR9I CCNGG 4 cut(s) 52, 96, 371, 458
MvaI CCWGG 4 cut(s) 52, 96, 371, 458
MvnI CGCG 1 cut(s) 123
NdeII GATC 3 cut(s) 76, 292, 497
NheI GCTAGC 1 cut(s) 446
NlaIII CATG 3 cut(s) 116, 233, 263
NlaIV GGNNCC 2 cut(s) 93, 499
NspI RCATGY 2 cut(s) 233, 263
NspV TTCGAA 1 cut(s) 336
PaeI GCATGC 1 cut(s) 263
PagI TCATGA 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 3 cut(s) 13, 44, 425
PkrI GCNGC 3 cut(s) 235, 433, 452
PpuMI RGGWCCY 1 cut(s) 92
Psp1406I AACGTT 1 cut(s) 475
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 4 cut(s) 50, 94, 369, 456
PspGI CCWGG 4 cut(s) 50, 94, 369, 456
PspN4I GGNNCC 2 cut(s) 93, 499
PspPI GGNCC 1 cut(s) 92
PspPPI RGGWCCY 1 cut(s) 92
PsuI RGATCY 1 cut(s) 497
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SatI GCNGC 3 cut(s) 234, 432, 451
Sau3AI GATC 3 cut(s) 76, 292, 497
Sau96I GGNCC 1 cut(s) 92
ScrFI CCNGG 4 cut(s) 52, 96, 371, 458
SexAI ACCWGGT 1 cut(s) 94
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 1 cut(s) 92
SphI GCATGC 1 cut(s) 263
Sse9I AATT 2 cut(s) 108, 332
SsiI CCGC 1 cut(s) 367
SspMI CTAG 2 cut(s) 447, 504
StyD4I CCNGG 4 cut(s) 50, 94, 369, 456
TaiI ACGT 2 cut(s) 21, 478
TaqI TCGA 3 cut(s) 42, 47, 336
TasI AATT 2 cut(s) 108, 332
TatI WGTACW 1 cut(s) 226
TfiI GAWTC 3 cut(s) 13, 44, 425
TseI GCWGC 3 cut(s) 233, 431, 450
TspDTI ATGAA 2 cut(s) 101, 399
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 1 cut(s) 108
XbaI TCTAGA 1 cut(s) 503
XceI RCATGY 2 cut(s) 233, 263
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 2 cut(s) 447, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.