Rh2AG516200

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004137
Physical Location & Seq
Forward (+)
74339522 .. 74342057
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG516200.1

Sequence Viewer

Length: 471 bp
ATGATAATGGCAGAGAAGATGGAGCTGCTGGGATTGTTAATGATGGGTTTTGTGGTCTTGTGCCAAATGAAAGGCTCCGTTGGGTTGAGGTTCGTGATAGATAGAGAAGAGTGCTTCTCTCATGAGGCAAAGTATGAGGGTGACACTGTTCATGTCTCTTTTGTCGTGATCAAAATCGATTCGACTTGGCATTACACTCAAGAAGGAGTTGATCTTGTGGTAAAGGGGCCTTCTGGTGAACAACTTCATGACATACGTGACAAGACGAGTGAGAAGTTTGAATTTGTGGTTCAGAAGGCCGGTGTTCATCACTTCTGCTTCACTAACAAGTCTCCTTACCATGAAACCATTGATTTTGATGTACATTCTGCACACTTCACATACTATGACGAGCATGCTAAAGATGGTGAGAGCCCATGTCTTATCATTACACATTTTGAAGTAATCTTTATGACAAAGAGTGATCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

156

Amino Acids

17.98

Weight (kDa)

5.47

Isoelectric Point (pI)

27.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 35 - 135 3.2e-15 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 281
AfaI GTAC 1 cut(s) 363
AgsI TTSAA 2 cut(s) 281, 440
AluBI AGCT 1 cut(s) 25
AluI AGCT 1 cut(s) 25
Alw26I GTCTC 2 cut(s) 160, 336
AoxI GGCC 2 cut(s) 227, 297
ApeKI GCWGC 1 cut(s) 25
ApoI RAATTY 1 cut(s) 281
Asp700I GAANNNNTTC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 3 cut(s) 152, 248, 419
BanII GRGCYC 1 cut(s) 416
BbvI GCAGC 1 cut(s) 12
BccI CCATC 3 cut(s) 13, 37, 398
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 160, 336
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 2 cut(s) 76, 228
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpuEI CTTGAG 1 cut(s) 183
Bsa29I ATCGAT 1 cut(s) 177
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 173
Bse118I RCCGGY 1 cut(s) 299
Bse8I GATNNNNATC 1 cut(s) 173
BseCI ATCGAT 1 cut(s) 177
BseJI GATNNNNATC 1 cut(s) 173
BseXI GCAGC 1 cut(s) 12
BseYI CCCAGC 1 cut(s) 28
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 2 cut(s) 229, 299
BshVI ATCGAT 1 cut(s) 177
BsiSI CCGG 1 cut(s) 300
BsmAI GTCTC 2 cut(s) 160, 336
BsnI GGCC 2 cut(s) 229, 299
Bsp1286I GDGCHC 1 cut(s) 416
Bsp1407I TGTACA 1 cut(s) 361
Bsp143I GATC 3 cut(s) 168, 211, 463
BspANI GGCC 2 cut(s) 229, 299
BspDI ATCGAT 1 cut(s) 177
BspHI TCATGA 2 cut(s) 121, 247
BspLI GGNNCC 2 cut(s) 76, 228
BsrFI RCCGGY 1 cut(s) 299
BsrGI TGTACA 1 cut(s) 361
BssAI RCCGGY 1 cut(s) 299
BssMI GATC 3 cut(s) 168, 211, 463
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 102
BstAUI TGTACA 1 cut(s) 361
BstBAI YACGTR 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 396
BstKTI GATC 3 cut(s) 171, 214, 466
BstMAI GTCTC 2 cut(s) 160, 336
BstMBI GATC 3 cut(s) 168, 211, 463
BstNSI RCATGY 1 cut(s) 398
BstV1I GCAGC 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 2 cut(s) 229, 299
BsuTUI ATCGAT 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 396
CciI TCATGA 2 cut(s) 121, 247
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 227
ClaI ATCGAT 1 cut(s) 177
Csp6I GTAC 1 cut(s) 362
CviAII CATG 6 cut(s) 122, 152, 248, 341, 395, 417
CviJI RGCY 5 cut(s) 25, 75, 229, 299, 414
CviKI_1 RGCY 5 cut(s) 25, 75, 229, 299, 414
CviQI GTAC 1 cut(s) 362
DpnI GATC 3 cut(s) 170, 213, 465
DpnII GATC 3 cut(s) 168, 211, 463
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 416
EcoO109I RGGNCCY 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 416
FaeI CATG 6 cut(s) 125, 155, 251, 344, 398, 420
FatI CATG 6 cut(s) 121, 151, 247, 340, 394, 416
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 1 cut(s) 26
FriOI GRGCYC 1 cut(s) 416
Fsp4HI GCNGC 1 cut(s) 26
GluI GCNGC 1 cut(s) 26
GsaI CCCAGC 1 cut(s) 32
HaeIII GGCC 2 cut(s) 229, 299
HapII CCGG 1 cut(s) 300
Hin1II CATG 6 cut(s) 125, 155, 251, 344, 398, 420
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 300
HphI GGTGA 3 cut(s) 152, 248, 419
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 1 cut(s) 294
Hpy188III TCNNGA 5 cut(s) 94, 122, 166, 200, 248
Hpy8I GTNNAC 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 197, 240, 289
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 1 cut(s) 371
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 6 cut(s) 125, 155, 251, 344, 398, 420
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 3 cut(s) 168, 211, 463
LmnI GCTCC 2 cut(s) 22, 80
LpnPI CCDG 3 cut(s) 14, 219, 313
Lsp1109I GCAGC 1 cut(s) 12
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 2 cut(s) 140, 257
MalI GATC 3 cut(s) 170, 213, 465
MboI GATC 3 cut(s) 168, 211, 463
MboII GAAGA 2 cut(s) 28, 119
MhlI GDGCHC 1 cut(s) 416
MluCI AATT 1 cut(s) 281
MnlI CCTC 3 cut(s) 81, 118, 130
MroXI GAANNNNTTC 1 cut(s) 243
MseI TTAA 1 cut(s) 38
MspI CCGG 1 cut(s) 300
NdeII GATC 3 cut(s) 168, 211, 463
NlaIII CATG 6 cut(s) 125, 155, 251, 344, 398, 420
NlaIV GGNNCC 2 cut(s) 76, 228
NmuCI GTSAC 2 cut(s) 140, 257
NspI RCATGY 1 cut(s) 398
PaeI GCATGC 1 cut(s) 398
PagI TCATGA 2 cut(s) 121, 247
PdmI GAANNNNTTC 1 cut(s) 243
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 1 cut(s) 27
Ppu21I YACGTR 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 76, 228
PspPI GGNCC 1 cut(s) 227
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 1 cut(s) 26
Sau3AI GATC 3 cut(s) 168, 211, 463
Sau96I GGNCC 1 cut(s) 227
SduI GDGCHC 1 cut(s) 416
SetI ASST 3 cut(s) 27, 92, 259
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SphI GCATGC 1 cut(s) 398
Sse9I AATT 1 cut(s) 281
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 259
TaqI TCGA 2 cut(s) 177, 182
TasI AATT 1 cut(s) 281
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 2 cut(s) 140, 257
TseI GCWGC 1 cut(s) 25
Tsp45I GTSAC 2 cut(s) 140, 257
TspDTI ATGAA 5 cut(s) 83, 140, 236, 296, 357
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 151
XapI RAATTY 1 cut(s) 281
XceI RCATGY 1 cut(s) 398
XmnI GAANNNNTTC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.