Rh5DG538100

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004137
Physical Location & Seq
Forward (+)
82999402 .. 83000728
1327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG538100.1

Sequence Viewer

Length: 477 bp
ATGCAAATTTCAAATTGTGCTGCTGTTACATTGTTGGCTTTTTTATTGAGCCTACAAGGAGCTTTTGGGATCAGATTTGTGATCGACAGAGATGAATGCTTCTCTCATAATGTTTTGTATGAAGGAGATACTGTCCACCTCTCTTTTGTTGTAATTAAGGTTGATGCATCTTGGCATTATACTCAAGACGGTGTTGATCTCCTGGTGAAGGGGCCTTCTGGTGAGCAGATACATGAGTTTCGTGATAAGACGAGCGAGAAATTCGAGTTTGTCTCTCGTCACAAAGGAATCCATCAGTTTTGTTTCACCAACAAGTCACCTTATCATGAAACCATAGACTTTGACTTACATGTCAGCCATTTTACATACTATGATCAGCATGCAAAAGATGGTGAGGCGCGGTACTTTTTGGACATCTTTATTGAAGTCTCTGATAACTGTTACACTGGTTGGAAAATAAGGCTGGACTTACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

158

Amino Acids

18.35

Weight (kDa)

5.46

Isoelectric Point (pI)

26.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 31 - 131 2.1e-14 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AccII CGCG 1 cut(s) 400
AciI CCGC 1 cut(s) 400
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 2 cut(s) 6, 260
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 208
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 2 cut(s) 12, 425
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 2 cut(s) 277, 433
AlwI GGATC 1 cut(s) 77
AoxI GGCC 1 cut(s) 212
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 2 cut(s) 6, 260
AspLEI GCGC 1 cut(s) 400
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 5 cut(s) 217, 233, 298, 309, 404
BaeI ACNNNNGTAYC 2 cut(s) 221, 254
BbvI GCAGC 1 cut(s) 7
BccI CCATC 2 cut(s) 300, 383
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 277, 433
BfmI CTRYAG 1 cut(s) 473
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 2 cut(s) 154, 176
BplI GAGNNNNNCTC 2 cut(s) 257, 289
BpuEI CTTGAG 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 451
BseBI CCWGG 1 cut(s) 203
BseLI CCNNNNNNNGG 1 cut(s) 208
BseNI ACTGG 1 cut(s) 451
BseXI GCAGC 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 400
BshFI GGCC 1 cut(s) 214
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 2 cut(s) 277, 433
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 1 cut(s) 214
Bsp143I GATC 4 cut(s) 69, 81, 196, 373
BspACI CCGC 1 cut(s) 400
BspANI GGCC 1 cut(s) 214
BspFNI CGCG 1 cut(s) 400
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 213
BspPI GGATC 1 cut(s) 77
BsrI ACTGG 1 cut(s) 451
BssMI GATC 4 cut(s) 69, 81, 196, 373
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 3 cut(s) 133, 191, 440
BstC8I GCNNGC 1 cut(s) 381
BstENI CCTNNNNNAGG 1 cut(s) 206
BstFNI CGCG 1 cut(s) 400
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 4 cut(s) 72, 84, 199, 376
BstMAI GTCTC 2 cut(s) 277, 433
BstMBI GATC 4 cut(s) 69, 81, 196, 373
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 2 cut(s) 353, 383
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 473
BstUI CGCG 1 cut(s) 400
BstV1I GCAGC 1 cut(s) 7
BsuRI GGCC 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 381
CciI TCATGA 1 cut(s) 325
CfoI GCGC 1 cut(s) 400
Cfr13I GGNCC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 403
CviAII CATG 4 cut(s) 233, 326, 350, 380
CviJI RGCY 6 cut(s) 38, 51, 62, 214, 357, 463
CviKI_1 RGCY 6 cut(s) 38, 51, 62, 214, 357, 463
CviQI GTAC 1 cut(s) 403
DpnI GATC 4 cut(s) 71, 83, 198, 375
DpnII GATC 4 cut(s) 69, 81, 196, 373
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 201
EcoT22I ATGCAT 1 cut(s) 169
FaeI CATG 4 cut(s) 236, 329, 353, 383
FatI CATG 4 cut(s) 232, 325, 349, 379
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 1 cut(s) 21
Fsp4HI GCNGC 1 cut(s) 21
GlaI GCGC 1 cut(s) 399
GluI GCNGC 1 cut(s) 21
HaeIII GGCC 1 cut(s) 214
HhaI GCGC 1 cut(s) 400
Hin1II CATG 4 cut(s) 236, 329, 353, 383
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HinfI GANTC 1 cut(s) 288
HphI GGTGA 5 cut(s) 217, 233, 298, 309, 404
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 74, 433
Hpy188III TCNNGA 3 cut(s) 185, 242, 326
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 3 cut(s) 116, 202, 225
HpyCH4III ACNGT 3 cut(s) 133, 191, 440
HpyCH4V TGCA 3 cut(s) 4, 167, 383
Hsp92II CATG 4 cut(s) 236, 329, 353, 383
HspAI GCGC 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 69, 81, 196, 373
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 5 cut(s) 188, 204, 215, 432, 449
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 2 cut(s) 154, 176
MaeIII GTNAC 4 cut(s) 25, 278, 315, 440
MalI GATC 4 cut(s) 71, 83, 198, 375
MboI GATC 4 cut(s) 69, 81, 196, 373
MluCI AATT 4 cut(s) 6, 13, 153, 260
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 2 cut(s) 149, 388
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 1 cut(s) 156
MspR9I CCNGG 1 cut(s) 203
Mva1269I GAATGC 1 cut(s) 101
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 400
NdeII GATC 4 cut(s) 69, 81, 196, 373
NlaIII CATG 4 cut(s) 236, 329, 353, 383
NlaIV GGNNCC 1 cut(s) 213
NmuCI GTSAC 2 cut(s) 278, 315
NsiI ATGCAT 1 cut(s) 169
NspI RCATGY 2 cut(s) 353, 383
PaeI GCATGC 1 cut(s) 383
PagI TCATGA 1 cut(s) 325
PciI ACATGT 1 cut(s) 349
PctI GAATGC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 288
PkrI GCNGC 1 cut(s) 22
PscI ACATGT 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 1 cut(s) 212
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 4 cut(s) 69, 81, 196, 373
Sau96I GGNCC 1 cut(s) 212
ScrFI CCNGG 1 cut(s) 203
SetI ASST 4 cut(s) 64, 141, 162, 322
SfaNI GCATC 2 cut(s) 154, 176
SfcI CTRYAG 1 cut(s) 473
SmlI CTYRAG 1 cut(s) 183
SmoI CTYRAG 1 cut(s) 183
SphI GCATGC 1 cut(s) 383
Sse9I AATT 4 cut(s) 6, 13, 153, 260
SsiI CCGC 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 133, 191, 440
TaqI TCGA 2 cut(s) 84, 264
TasI AATT 4 cut(s) 6, 13, 153, 260
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 2 cut(s) 278, 315
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 2 cut(s) 278, 315
TspDTI ATGAA 3 cut(s) 108, 135, 342
TspRI CASTG 1 cut(s) 451
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 2 cut(s) 6, 260
XceI RCATGY 2 cut(s) 353, 383
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.