Prupe.3G196600_v2.0.a1

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0004137
Physical Location & Seq
Forward (+)
20729138 .. 20731079
1942 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G196600.2

Sequence Viewer

Length: 651 bp
ATGGAGTTGTGGGTCAGGGGACACGTGGCACTGGGTTTGGCGCTGATGGGTTTGCTGGTTTTGTGCCAAATGGAAGGGTCAGATGGGTTGAGGTTCGTGATAGATAGAGAGGAGTGCTTCTCTCATGATGCAAAGTACCAGGGCGACACCATTCACGTCTCTTTTGTTGTCATCAAAGTTGATTCGTCTTGGCATAACACTGAAGATGGTGTTGATCTTGTGATAAAGGGGCCTTCTGGTGAACAAATTAATGACATACGTGACAAGACAAGCGAGAAGTTTGAGTTTGTGGTTCAGAAGCAAGGTGTTCACCAGTTCTGCTTCACTAATAAGTCTCCTTACCATGAAACCATTGACTTTGATGTACATGCTGCTCACTTTACTTACTATGACCAGCATGCTAAAGATGAGCATTTTGCACCCTTGCTGGAGCAGATAGCGAAATTGGAGGAAGCCCTTTTCAACATCCAATTTGAACAACATTGGTTAGAGGCCGAGACTGACCGCCAGGCAATAGTGAATGAAGGAATGAGCCGCAGAGCAACCCACAAGGCGTTAGTTGAATCAGGAGCACTCATTGGGGTTAGCATCCTCCAAGTTGTTCTCTTGAAACGTTTGTTTGAACGCAAGCTTGGATCATCTAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

217

Amino Acids

24.64

Weight (kDa)

5.5

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 505, 535
AclI AACGTT 1 cut(s) 613
AclWI GGATC 1 cut(s) 643
AcuI CTGAAG 1 cut(s) 222
AcvI CACGTG 1 cut(s) 25
AfaI GTAC 2 cut(s) 137, 366
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 5 cut(s) 463, 476, 563, 610, 623
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 2 cut(s) 138, 507
AjuI GAANNNNNNNTTGG 2 cut(s) 615, 647
AluBI AGCT 1 cut(s) 631
AluI AGCT 1 cut(s) 631
Alw21I GWGCWC 1 cut(s) 574
Alw26I GTCTC 3 cut(s) 163, 339, 491
AlwI GGATC 1 cut(s) 643
AoxI GGCC 2 cut(s) 230, 492
ApeKI GCWGC 1 cut(s) 371
AseI ATTAAT 1 cut(s) 249
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 251, 302
BbrPI CACGTG 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 574
BbvI GCAGC 1 cut(s) 358
BccI CCATC 3 cut(s) 40, 77, 200
BciT130I CCWGG 2 cut(s) 140, 509
BcoDI GTCTC 3 cut(s) 163, 339, 491
BfaI CTAG 1 cut(s) 642
BfoI RGCGCY 1 cut(s) 44
BisI GCNGC 2 cut(s) 372, 535
BlsI GCNGC 2 cut(s) 373, 536
Bme1390I CCNGG 2 cut(s) 140, 509
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 2 cut(s) 140, 509
BmrI ACTGGG 1 cut(s) 41
BmsI GCATC 2 cut(s) 118, 597
BmuI ACTGGG 1 cut(s) 41
BplI GAGNNNNNCTC 2 cut(s) 104, 136
BpmI CTGGAG 1 cut(s) 449
BsaAI YACGTR 2 cut(s) 25, 260
BsaJI CCNNGG 1 cut(s) 139
Bse1I ACTGG 2 cut(s) 36, 313
BseBI CCWGG 2 cut(s) 140, 509
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 2 cut(s) 465, 588
BseNI ACTGG 2 cut(s) 36, 313
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 358
BshFI GGCC 2 cut(s) 232, 494
BsiHKAI GWGCWC 1 cut(s) 574
BslFI GGGAC 1 cut(s) 33
BsmAI GTCTC 3 cut(s) 163, 339, 491
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 2 cut(s) 232, 494
Bsp1286I GDGCHC 1 cut(s) 574
Bsp1407I TGTACA 1 cut(s) 364
Bsp143I GATC 2 cut(s) 214, 635
BspACI CCGC 2 cut(s) 505, 535
BspANI GGCC 2 cut(s) 232, 494
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 1 cut(s) 643
BsrGI TGTACA 1 cut(s) 364
BsrI ACTGG 2 cut(s) 36, 313
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 2 cut(s) 214, 635
Bst2UI CCWGG 2 cut(s) 140, 509
BstAUI TGTACA 1 cut(s) 364
BstBAI YACGTR 2 cut(s) 25, 260
BstC8I GCNNGC 2 cut(s) 399, 629
BstF5I GGATG 2 cut(s) 465, 588
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 2 cut(s) 217, 638
BstMAI GTCTC 3 cut(s) 163, 339, 491
BstMBI GATC 2 cut(s) 214, 635
BstNI CCWGG 2 cut(s) 140, 509
BstNSI RCATGY 2 cut(s) 371, 401
BstSCI CCNGG 2 cut(s) 138, 507
BstV1I GCAGC 1 cut(s) 358
BsuRI GGCC 2 cut(s) 232, 494
BtrI CACGTC 1 cut(s) 157
BtsCI GGATG 2 cut(s) 465, 588
BtsIMutI CAGTG 2 cut(s) 29, 198
Cac8I GCNNGC 2 cut(s) 399, 629
CciI TCATGA 1 cut(s) 124
CfoI GCGC 1 cut(s) 43
Cfr13I GGNCC 1 cut(s) 230
Csp6I GTAC 2 cut(s) 136, 365
CviAII CATG 4 cut(s) 125, 344, 368, 398
CviJI RGCY 5 cut(s) 232, 455, 494, 534, 631
CviKI_1 RGCY 5 cut(s) 232, 455, 494, 534, 631
CviQI GTAC 2 cut(s) 136, 365
DpnI GATC 2 cut(s) 216, 637
DpnII GATC 2 cut(s) 214, 635
Eco57I CTGAAG 1 cut(s) 222
Eco72I CACGTG 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 2 cut(s) 138, 507
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 4 cut(s) 128, 347, 371, 401
FaiI YATR 7 cut(s) 126, 195, 257, 345, 369, 390, 399
FaqI GGGAC 1 cut(s) 33
FatI CATG 4 cut(s) 124, 343, 367, 397
Fnu4HI GCNGC 2 cut(s) 372, 535
FokI GGATG 2 cut(s) 452, 575
Fsp4HI GCNGC 2 cut(s) 372, 535
FspBI CTAG 1 cut(s) 642
GlaI GCGC 1 cut(s) 42
GluI GCNGC 2 cut(s) 372, 535
GsuI CTGGAG 1 cut(s) 449
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 2 cut(s) 232, 494
HhaI GCGC 1 cut(s) 43
Hin1II CATG 4 cut(s) 128, 347, 371, 401
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 2 cut(s) 182, 563
HphI GGTGA 2 cut(s) 251, 302
Hpy166II GTNNAC 2 cut(s) 242, 310
Hpy188I TCNGA 2 cut(s) 82, 297
Hpy188III TCNNGA 4 cut(s) 97, 125, 567, 607
Hpy8I GTNNAC 2 cut(s) 242, 310
HpyAV CCTTC 3 cut(s) 68, 243, 518
HpyCH4IV ACGT 4 cut(s) 24, 156, 259, 613
HpyCH4V TGCA 2 cut(s) 131, 419
HpySE526I ACGT 4 cut(s) 24, 156, 259, 613
Hsp92II CATG 4 cut(s) 128, 347, 371, 401
HspAI GCGC 1 cut(s) 41
Kzo9I GATC 2 cut(s) 214, 635
LmnI GCTCC 2 cut(s) 430, 569
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 2 cut(s) 118, 597
MaeI CTAG 1 cut(s) 642
MaeII ACGT 4 cut(s) 24, 156, 259, 613
MaeIII GTNAC 1 cut(s) 260
MalI GATC 2 cut(s) 216, 637
MboI GATC 2 cut(s) 214, 635
MboII GAAGA 1 cut(s) 215
MhlI GDGCHC 1 cut(s) 574
MluCI AATT 3 cut(s) 246, 443, 470
MnlI CCTC 5 cut(s) 84, 103, 442, 484, 602
MseI TTAA 1 cut(s) 249
MspR9I CCNGG 2 cut(s) 140, 509
MvaI CCWGG 2 cut(s) 140, 509
NdeII GATC 2 cut(s) 214, 635
NlaIII CATG 4 cut(s) 128, 347, 371, 401
NlaIV GGNNCC 1 cut(s) 231
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 1 cut(s) 260
NspI RCATGY 2 cut(s) 371, 401
PaeI GCATGC 1 cut(s) 401
PagI TCATGA 1 cut(s) 124
PfeI GAWTC 2 cut(s) 182, 563
PkrI GCNGC 2 cut(s) 373, 536
PmaCI CACGTG 1 cut(s) 25
PmlI CACGTG 1 cut(s) 25
Ppu21I YACGTR 2 cut(s) 25, 260
PshBI ATTAAT 1 cut(s) 249
Psp1406I AACGTT 1 cut(s) 613
Psp6I CCWGG 2 cut(s) 138, 507
PspCI CACGTG 1 cut(s) 25
PspGI CCWGG 2 cut(s) 138, 507
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 230
RsaI GTAC 2 cut(s) 137, 366
RsaNI GTAC 2 cut(s) 136, 365
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 2 cut(s) 372, 535
Sau3AI GATC 2 cut(s) 214, 635
Sau96I GGNCC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 140, 509
SduI GDGCHC 1 cut(s) 574
SetI ASST 7 cut(s) 27, 95, 159, 262, 307, 616, 633
SfaNI GCATC 2 cut(s) 118, 597
SphI GCATGC 1 cut(s) 401
Sse9I AATT 3 cut(s) 246, 443, 470
SsiI CCGC 2 cut(s) 505, 535
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 138, 507
TaiI ACGT 4 cut(s) 27, 159, 262, 616
TasI AATT 3 cut(s) 246, 443, 470
TatI WGTACW 1 cut(s) 364
TauI GCSGC 1 cut(s) 537
TfiI GAWTC 2 cut(s) 182, 563
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 2 cut(s) 36, 205
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 2 cut(s) 360, 537
TspRI CASTG 2 cut(s) 36, 205
VspI ATTAAT 1 cut(s) 249
XceI RCATGY 2 cut(s) 371, 401
XspI CTAG 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.