FvH4_6g40540

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004137
Physical Location & Seq
Forward (+)
32025487 .. 32027477
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g40540.t1

Sequence Viewer

Length: 630 bp
ATGGGGCTGCTGGGATTGTTAATGATGGGTTTTGTGGTCTTGTGCCAAATGAAAGGCTCCGCTGGGTTGAGGATCGTGATAGATAGAGAAGAGTGCTTCTCTCATGATGCAAAGTATGAAGGTGACACTGTTCATGTCTCTTTTGTTGTTATCAAAATCGATTCGAGTTGGCATTACACTCAAGATGGAGTTGATCTTGTGGTAAAGGGGCCTTCTGGCGAACAACTTCATGATATACGTGATAAAACGAGTGAGAAGTTTGAATTTGTGGTTCAGAAGGCTGGTGTTCATCACTTCTGCTTCACAAACAAGTCTCCTTACCATGAAACCATTGATTTTGATGTACATTCTGCACACTTTACATACTATGATGAGCATGCTAAAGATGAGCATTTCAAACCTTTGTTCGAGCAGATTCAAAAGTTGGAGGAAGCCATTTTCAACATCCAATTTGAACAACACTGGCTAGAGGCTGAGACTGACCGCCAAGCAATATTGAATGAAGGAATGAGTCGGAGAGCAACTCACAAGGCAATGTTTGAGTCGGCCGCACTTATTGGAACCAGTTTCCTCCAAGTATTTCTTTTGAAACGTTTGTTTGAGCGCAAGCTTGGATCATCTAGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

210

Amino Acids

24.11

Weight (kDa)

5.88

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 29 - 201 1.8e-33 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 60, 484, 549
AclI AACGTT 1 cut(s) 592
AclWI GGATC 2 cut(s) 80, 622
AcoI YGGCCR 1 cut(s) 546
AcsI RAATTY 1 cut(s) 263
AfaI GTAC 1 cut(s) 345
AgsI TTSAA 7 cut(s) 263, 397, 419, 442, 455, 499, 589
AluBI AGCT 1 cut(s) 610
AluI AGCT 1 cut(s) 610
Alw26I GTCTC 3 cut(s) 142, 318, 470
AlwI GGATC 2 cut(s) 80, 622
AoxI GGCC 2 cut(s) 209, 546
ApeKI GCWGC 1 cut(s) 7
ApoI RAATTY 1 cut(s) 263
Asp700I GAANNNNTTC 1 cut(s) 225
AspLEI GCGC 1 cut(s) 606
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 134
BccI CCATC 2 cut(s) 19, 179
BcoDI GTCTC 3 cut(s) 142, 318, 470
BfaI CTAG 3 cut(s) 467, 621, 628
BisI GCNGC 2 cut(s) 8, 549
BlsI GCNGC 2 cut(s) 9, 550
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 3 cut(s) 58, 210, 562
BmsI GCATC 1 cut(s) 97
BplI GAGNNNNNCTC 4 cut(s) 83, 115, 508, 540
BpuEI CTTGAG 1 cut(s) 165
Bsa29I ATCGAT 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 239
Bse1I ACTGG 2 cut(s) 467, 564
Bse3DI GCAATG 1 cut(s) 540
BseCI ATCGAT 1 cut(s) 159
BseGI GGATG 1 cut(s) 444
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 2 cut(s) 467, 564
BseX3I CGGCCG 1 cut(s) 546
BseYI CCCAGC 2 cut(s) 10, 62
BsgI GTGCAG 1 cut(s) 336
Bsh1285I CGRYCG 1 cut(s) 549
BshFI GGCC 2 cut(s) 211, 548
BshVI ATCGAT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 549
BsmAI GTCTC 3 cut(s) 142, 318, 470
BsnI GGCC 2 cut(s) 211, 548
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 3 cut(s) 72, 193, 614
BspACI CCGC 3 cut(s) 60, 484, 549
BspANI GGCC 2 cut(s) 211, 548
BspCNI CTCAG 1 cut(s) 466
BspDI ATCGAT 1 cut(s) 159
BspHI TCATGA 2 cut(s) 103, 229
BspLI GGNNCC 3 cut(s) 58, 210, 562
BspPI GGATC 2 cut(s) 80, 622
BsrDI GCAATG 1 cut(s) 540
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 2 cut(s) 467, 564
BssMI GATC 3 cut(s) 72, 193, 614
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 1 cut(s) 84
BstAUI TGTACA 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 378, 608
BstDEI CTNAG 1 cut(s) 474
BstF5I GGATG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 606
BstKTI GATC 3 cut(s) 75, 196, 617
BstMAI GTCTC 3 cut(s) 142, 318, 470
BstMBI GATC 3 cut(s) 72, 193, 614
BstMCI CGRYCG 1 cut(s) 549
BstNSI RCATGY 1 cut(s) 380
BstZI CGGCCG 1 cut(s) 546
Bsu15I ATCGAT 1 cut(s) 159
BsuRI GGCC 2 cut(s) 211, 548
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 1 cut(s) 444
BtsIMutI CAGTG 2 cut(s) 126, 460
Cac8I GCNNGC 2 cut(s) 378, 608
CciI TCATGA 2 cut(s) 103, 229
CfoI GCGC 1 cut(s) 606
Cfr13I GGNCC 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 1 cut(s) 344
CviAII CATG 5 cut(s) 104, 134, 230, 323, 377
CviJI RGCY 9 cut(s) 7, 57, 211, 281, 434, 466, 473, 548, 610
CviKI_1 RGCY 9 cut(s) 7, 57, 211, 281, 434, 466, 473, 548, 610
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 1 cut(s) 474
DpnI GATC 3 cut(s) 74, 195, 616
DpnII GATC 3 cut(s) 72, 193, 614
EaeI YGGCCR 1 cut(s) 546
EagI CGGCCG 1 cut(s) 546
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
EclXI CGGCCG 1 cut(s) 546
Eco52I CGGCCG 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 209
FaeI CATG 5 cut(s) 107, 137, 233, 326, 380
FaiI YATR 9 cut(s) 105, 117, 135, 231, 236, 324, 364, 369, 378
FalI AAGNNNNNCTT 2 cut(s) 567, 599
FatI CATG 5 cut(s) 103, 133, 229, 322, 376
Fnu4HI GCNGC 2 cut(s) 8, 549
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 2 cut(s) 8, 549
FspBI CTAG 3 cut(s) 467, 621, 628
GlaI GCGC 1 cut(s) 605
GluI GCNGC 2 cut(s) 8, 549
GsaI CCCAGC 2 cut(s) 14, 66
HaeIII GGCC 2 cut(s) 211, 548
HhaI GCGC 1 cut(s) 606
Hin1II CATG 5 cut(s) 107, 137, 233, 326, 380
Hin6I GCGC 1 cut(s) 604
HinP1I GCGC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 5 cut(s) 161, 415, 511, 542, 624
HphI GGTGA 1 cut(s) 134
Hpy188I TCNGA 2 cut(s) 276, 516
Hpy188III TCNNGA 5 cut(s) 76, 104, 182, 230, 621
HpyAV CCTTC 4 cut(s) 113, 222, 271, 497
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 238, 592
HpyCH4V TGCA 2 cut(s) 110, 353
HpyF3I CTNAG 1 cut(s) 474
HpySE526I ACGT 2 cut(s) 238, 592
Hsp92II CATG 5 cut(s) 107, 137, 233, 326, 380
HspAI GCGC 1 cut(s) 604
Kzo9I GATC 3 cut(s) 72, 193, 614
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 5 cut(s) 48, 201, 267, 448, 577
LweI GCATC 1 cut(s) 97
MaeI CTAG 3 cut(s) 467, 621, 628
MaeII ACGT 2 cut(s) 238, 592
MaeIII GTNAC 1 cut(s) 122
MalI GATC 3 cut(s) 74, 195, 616
MboI GATC 3 cut(s) 72, 193, 614
MboII GAAGA 1 cut(s) 101
MluCI AATT 2 cut(s) 263, 449
MlyI GAGTC 2 cut(s) 520, 551
MmeI TCCRAC 2 cut(s) 405, 494
MnlI CCTC 4 cut(s) 63, 421, 463, 581
MroXI GAANNNNTTC 1 cut(s) 225
MseI TTAA 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 62
NdeII GATC 3 cut(s) 72, 193, 614
NlaIII CATG 5 cut(s) 107, 137, 233, 326, 380
NlaIV GGNNCC 3 cut(s) 58, 210, 562
NmuCI GTSAC 1 cut(s) 122
NspI RCATGY 1 cut(s) 380
PaeI GCATGC 1 cut(s) 380
PagI TCATGA 2 cut(s) 103, 229
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 2 cut(s) 161, 415
PkrI GCNGC 2 cut(s) 9, 550
PleI GAGTC 2 cut(s) 519, 550
PpsI GAGTC 2 cut(s) 519, 550
Ppu21I YACGTR 1 cut(s) 239
Psp1406I AACGTT 1 cut(s) 592
PspFI CCCAGC 2 cut(s) 10, 62
PspN4I GGNNCC 3 cut(s) 58, 210, 562
PspPI GGNCC 1 cut(s) 209
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 2 cut(s) 8, 549
Sau3AI GATC 3 cut(s) 72, 193, 614
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 2 cut(s) 520, 551
SetI ASST 5 cut(s) 124, 241, 403, 595, 612
SfaNI GCATC 1 cut(s) 97
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
SphI GCATGC 1 cut(s) 380
Sse9I AATT 2 cut(s) 263, 449
SsiI CCGC 3 cut(s) 60, 484, 549
SspI AATATT 1 cut(s) 495
SspMI CTAG 3 cut(s) 467, 621, 628
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 2 cut(s) 241, 595
TaqI TCGA 3 cut(s) 159, 164, 408
TasI AATT 2 cut(s) 263, 449
TatI WGTACW 1 cut(s) 343
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 2 cut(s) 161, 415
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 2 cut(s) 133, 467
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 7 cut(s) 65, 122, 132, 218, 278, 339, 516
TspRI CASTG 2 cut(s) 133, 467
XapI RAATTY 1 cut(s) 263
XbaI TCTAGA 1 cut(s) 620
XceI RCATGY 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 3 cut(s) 467, 621, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.