Rh2CG501100

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004137
Physical Location & Seq
Forward (+)
67308665 .. 67310138
1474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG501100.1

Sequence Viewer

Length: 642 bp
ATGGCAGAGAAGATGGAGCTGCTGGGATTGTTAATGATGGGTTTTGTGGTCTTGTGCCAAATGAAAGGCTCCGTTGGGTTGAGGTTCGTGATAGATAGAGAAGAGTGCTTCTCTCATGAGGCAAAGTATGAGGGTGACACTGTTCATGTCTCTTTTGTCGTGATCAAAATCGATTCGACTTGGCATTACACTCAAGAAGGAGTTGATCTTGTGGTAAAGGGGCCTTCTGGTGAACAACTTCATGACATACGTGACAAGACGAGTGAGAAGTTTGAATTTGTGGTTCAGAAGGCCGGTGTTCATCACTTCTGCTTCACTAACAAGTCTCCTTACCATGAAACCATTGATTTTGATGTACATTCTGCACACTTCACATACTATGACGAGCATGCTAAAGATGAGCATTTCAAACCCTTGTTTGAGCAGATTCAAAAGTTGGAGGAAGCCATTTTCAATATCCAATTTGAACAACACTGGCTAGAGGCTGAGACTGACCGCCAGGCAATATTGAATGAAGGAATGAGTCAGAGGGCAACTCACAAGGCAATGTTTGAATCAGCTGCACTTATTGGAACCAGCCTCCTCCAAGTTTTTCTTTTGAAACGTTTGTTTGAGCGCAAGCTTGGGTCATCTAGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

213

Amino Acids

24.69

Weight (kDa)

5.69

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 33 - 205 1.1e-33 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 496
AclI AACGTT 1 cut(s) 604
AcsI RAATTY 1 cut(s) 275
AfaI GTAC 1 cut(s) 357
AgsI TTSAA 8 cut(s) 275, 409, 431, 454, 467, 511, 554, 601
AjnI CCWGG 1 cut(s) 498
AluBI AGCT 3 cut(s) 19, 560, 622
AluI AGCT 3 cut(s) 19, 560, 622
Alw26I GTCTC 3 cut(s) 154, 330, 482
AoxI GGCC 2 cut(s) 221, 291
ApeKI GCWGC 2 cut(s) 19, 560
ApoI RAATTY 1 cut(s) 275
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 618
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 2 cut(s) 146, 242
BbvI GCAGC 2 cut(s) 6, 547
BccI CCATC 2 cut(s) 7, 31
BciT130I CCWGG 1 cut(s) 500
BclI TGATCA 1 cut(s) 162
BcoDI GTCTC 3 cut(s) 154, 330, 482
BfaI CTAG 3 cut(s) 479, 633, 640
BisI GCNGC 2 cut(s) 20, 561
BlsI GCNGC 2 cut(s) 21, 562
Bme1390I CCNGG 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 3 cut(s) 70, 222, 574
BmrFI CCNGG 1 cut(s) 500
BplI GAGNNNNNCTC 4 cut(s) 95, 127, 520, 552
BpuEI CTTGAG 1 cut(s) 177
Bsa29I ATCGAT 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 251
BsaBI GATNNNNATC 1 cut(s) 167
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 479
Bse3DI GCAATG 1 cut(s) 552
Bse8I GATNNNNATC 1 cut(s) 167
BseBI CCWGG 1 cut(s) 500
BseCI ATCGAT 1 cut(s) 171
BseJI GATNNNNATC 1 cut(s) 167
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 479
BseRI GAGGAG 1 cut(s) 572
BseXI GCAGC 2 cut(s) 6, 547
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 2 cut(s) 348, 546
BshFI GGCC 2 cut(s) 223, 293
BshVI ATCGAT 1 cut(s) 171
BsiSI CCGG 1 cut(s) 294
BsmAI GTCTC 3 cut(s) 154, 330, 482
BsnI GGCC 2 cut(s) 223, 293
Bsp1407I TGTACA 1 cut(s) 355
Bsp143I GATC 2 cut(s) 162, 205
BspACI CCGC 1 cut(s) 496
BspANI GGCC 2 cut(s) 223, 293
BspCNI CTCAG 1 cut(s) 478
BspDI ATCGAT 1 cut(s) 171
BspHI TCATGA 2 cut(s) 115, 241
BspLI GGNNCC 3 cut(s) 70, 222, 574
BsrDI GCAATG 1 cut(s) 552
BsrFI RCCGGY 1 cut(s) 293
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 1 cut(s) 479
BssAI RCCGGY 1 cut(s) 293
BssMI GATC 2 cut(s) 162, 205
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 96
BstAUI TGTACA 1 cut(s) 355
BstBAI YACGTR 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 390, 620
BstDEI CTNAG 1 cut(s) 486
BstHHI GCGC 1 cut(s) 618
BstKTI GATC 2 cut(s) 165, 208
BstMAI GTCTC 3 cut(s) 154, 330, 482
BstMBI GATC 2 cut(s) 162, 205
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 498
BstV1I GCAGC 2 cut(s) 6, 547
Bsu15I ATCGAT 1 cut(s) 171
BsuRI GGCC 2 cut(s) 223, 293
BsuTUI ATCGAT 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 138, 472
Cac8I GCNNGC 2 cut(s) 390, 620
CciI TCATGA 2 cut(s) 115, 241
CfoI GCGC 1 cut(s) 618
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 221
ClaI ATCGAT 1 cut(s) 171
Csp6I GTAC 1 cut(s) 356
CviAII CATG 5 cut(s) 116, 146, 242, 335, 389
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 1 cut(s) 486
DpnI GATC 2 cut(s) 164, 207
DpnII GATC 2 cut(s) 162, 205
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 498
FaeI CATG 5 cut(s) 119, 149, 245, 338, 392
FaiI YATR 9 cut(s) 117, 129, 147, 243, 248, 336, 376, 381, 390
FalI AAGNNNNNCTT 2 cut(s) 579, 611
FatI CATG 5 cut(s) 115, 145, 241, 334, 388
FbaI TGATCA 1 cut(s) 162
Fnu4HI GCNGC 2 cut(s) 20, 561
Fsp4HI GCNGC 2 cut(s) 20, 561
FspBI CTAG 3 cut(s) 479, 633, 640
GlaI GCGC 1 cut(s) 617
GluI GCNGC 2 cut(s) 20, 561
GsaI CCCAGC 1 cut(s) 26
HaeIII GGCC 2 cut(s) 223, 293
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 618
Hin1II CATG 5 cut(s) 119, 149, 245, 338, 392
Hin6I GCGC 1 cut(s) 616
HinP1I GCGC 1 cut(s) 616
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 5 cut(s) 173, 427, 523, 554, 636
HpaII CCGG 1 cut(s) 294
HphI GGTGA 2 cut(s) 146, 242
Hpy166II GTNNAC 1 cut(s) 233
Hpy188I TCNGA 2 cut(s) 288, 528
Hpy188III TCNNGA 6 cut(s) 88, 116, 160, 194, 242, 633
Hpy8I GTNNAC 1 cut(s) 233
HpyAV CCTTC 4 cut(s) 191, 234, 283, 509
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 2 cut(s) 250, 604
HpyCH4V TGCA 2 cut(s) 365, 563
HpyF3I CTNAG 1 cut(s) 486
HpySE526I ACGT 2 cut(s) 250, 604
Hsp92II CATG 5 cut(s) 119, 149, 245, 338, 392
HspAI GCGC 1 cut(s) 616
Ksp22I TGATCA 1 cut(s) 162
Kzo9I GATC 2 cut(s) 162, 205
LmnI GCTCC 2 cut(s) 16, 74
LpnPI CCDG 7 cut(s) 8, 213, 307, 460, 485, 512, 589
Lsp1109I GCAGC 2 cut(s) 6, 547
MaeI CTAG 3 cut(s) 479, 633, 640
MaeII ACGT 2 cut(s) 250, 604
MaeIII GTNAC 2 cut(s) 134, 251
MalI GATC 2 cut(s) 164, 207
MboI GATC 2 cut(s) 162, 205
MboII GAAGA 2 cut(s) 22, 113
MluCI AATT 2 cut(s) 275, 461
MlyI GAGTC 1 cut(s) 532
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 8 cut(s) 75, 112, 124, 433, 475, 522, 590, 593
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 560
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 500
MvaI CCWGG 1 cut(s) 500
NdeII GATC 2 cut(s) 162, 205
NlaIII CATG 5 cut(s) 119, 149, 245, 338, 392
NlaIV GGNNCC 3 cut(s) 70, 222, 574
NmuCI GTSAC 2 cut(s) 134, 251
NspI RCATGY 1 cut(s) 392
PaeI GCATGC 1 cut(s) 392
PagI TCATGA 2 cut(s) 115, 241
PdmI GAANNNNTTC 1 cut(s) 237
PfeI GAWTC 3 cut(s) 173, 427, 554
PkrI GCNGC 2 cut(s) 21, 562
PleI GAGTC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 531
Ppu21I YACGTR 1 cut(s) 251
Psp1406I AACGTT 1 cut(s) 604
Psp6I CCWGG 1 cut(s) 498
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 3 cut(s) 70, 222, 574
PspPI GGNCC 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 2 cut(s) 20, 561
Sau3AI GATC 2 cut(s) 162, 205
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 1 cut(s) 532
ScrFI CCNGG 1 cut(s) 500
SetI ASST 6 cut(s) 21, 86, 253, 562, 607, 624
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
SphI GCATGC 1 cut(s) 392
Sse9I AATT 2 cut(s) 275, 461
SsiI CCGC 1 cut(s) 496
SspI AATATT 1 cut(s) 507
SspMI CTAG 3 cut(s) 479, 633, 640
StyD4I CCNGG 1 cut(s) 498
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 2 cut(s) 253, 607
TaqI TCGA 2 cut(s) 171, 176
TasI AATT 2 cut(s) 275, 461
TatI WGTACW 1 cut(s) 355
TfiI GAWTC 3 cut(s) 173, 427, 554
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 2 cut(s) 145, 479
TseFI GTSAC 2 cut(s) 134, 251
TseI GCWGC 2 cut(s) 19, 560
Tsp45I GTSAC 2 cut(s) 134, 251
TspDTI ATGAA 6 cut(s) 77, 134, 230, 290, 351, 528
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 2 cut(s) 145, 479
XapI RAATTY 1 cut(s) 275
XbaI TCTAGA 1 cut(s) 632
XceI RCATGY 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 3 cut(s) 479, 633, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.