Rroxscaffold_1G00005200

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004137
Physical Location & Seq
Reverse (-)
7043044 .. 7045535
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005200.1

Sequence Viewer

Length: 750 bp
ATGCCGATCAACACTCTCACCGACTTCAAGAAATGCGACTCGGAGAGAGCCCCGACCACCATAGAGAGCTCCTCTGTGAAGAGTAGAAGGAGGCTTGCGAGAATCCCATGGTTTTTGATGCAAATTTCAAATTGTGCTGCTGTTACATTGTTGGCTTTTTTACTGAGCCTACAAGGAGCTTTTGGGATCAGATTTGTGATCGACAGAGATGAATGCTTCTCTCATAATGTTTTGTATGAAGGAGATACTGTCCACCTCTCTTTTGTTGTAATTAAGGTTGATGCATCTTGGCATTATACTCAAGACGGTGTTGATCTCCTGGTGAAGGGGCCTTCTGGTGAGCAAATACATGAGTTTCGTGATAAGACAAGCGAGAAGTTTGAGTTTGTCACTCGTCACAAAGGAATCCATCAGTTCTGTTTCACCAACAAGTCACCTTATCATGAAACCATAGACTTTGACTTACATGTCAGCCATTTTACATACTATGATCAGCATGCAAAAGATGAGCATTTTAACCCTTTGTTAGATCAGATAACAAAGTTAGAGGAAGCTCTTTACAACATTCAGTTTGAGCAGCATTGGCTTGAGGCTCAGACTGAACGCCAGGCAATAGTGAACGACGCAATGAGCCGGAGGGCGATTCACAAGGCCTTTTTTGAATCAGCAGGACTTGTTGGTGCCAGTGTTCTCCAAGTTTACCTTCTTCGCCGCCTCTTTGAACGGAAGCTTGGGATGTCCAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

249

Amino Acids

28.92

Weight (kDa)

6.49

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 70 - 241 7.2e-32 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 680
AciI CCGC 1 cut(s) 712
AclWI GGATC 1 cut(s) 194
AcsI RAATTY 1 cut(s) 123
AfiI CCNNNNNNNGG 1 cut(s) 325
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 4 cut(s) 28, 129, 662, 722
AjnI CCWGG 2 cut(s) 318, 606
AjuI GAANNNNNNNTTGG 4 cut(s) 687, 714, 719, 746
AluBI AGCT 4 cut(s) 69, 179, 554, 730
AluI AGCT 4 cut(s) 69, 179, 554, 730
Alw21I GWGCWC 1 cut(s) 71
AlwI GGATC 1 cut(s) 194
AlwNI CAGNNNCTG 1 cut(s) 747
AoxI GGCC 2 cut(s) 329, 651
ApeKI GCWGC 2 cut(s) 137, 577
ApoI RAATTY 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 5 cut(s) 10, 334, 350, 415, 426
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 2 cut(s) 52, 71
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 2 cut(s) 124, 589
BccI CCATC 1 cut(s) 417
BciT130I CCWGG 2 cut(s) 320, 608
BclI TGATCA 1 cut(s) 490
BisI GCNGC 3 cut(s) 138, 578, 712
BlsI GCNGC 3 cut(s) 139, 579, 713
Bme1390I CCNGG 2 cut(s) 320, 608
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 2 cut(s) 330, 682
BmrFI CCNGG 2 cut(s) 320, 608
BmsI GCATC 3 cut(s) 108, 271, 293
BplI GAGNNNNNCTC 2 cut(s) 56, 88
BpuEI CTTGAG 2 cut(s) 285, 608
BsaJI CCNNGG 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 684
Bse3DI GCAATG 1 cut(s) 633
BseBI CCWGG 2 cut(s) 320, 608
BseDI CCNNGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 741
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 633
BseMII CTCAG 2 cut(s) 155, 608
BseNI ACTGG 1 cut(s) 684
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 124, 589
BshFI GGCC 2 cut(s) 331, 653
BshNI GGYRCC 1 cut(s) 680
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 634
BslI CCNNNNNNNGG 1 cut(s) 325
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 2 cut(s) 331, 653
Bsp1286I GDGCHC 2 cut(s) 52, 71
Bsp143I GATC 6 cut(s) 6, 186, 198, 313, 490, 529
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 1 cut(s) 712
BspANI GGCC 2 cut(s) 331, 653
BspCNI CTCAG 2 cut(s) 156, 607
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 330, 682
BspPI GGATC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 633
BsrI ACTGG 1 cut(s) 684
BssECI CCNNGG 1 cut(s) 107
BssMI GATC 6 cut(s) 6, 186, 198, 313, 490, 529
BssT1I CCWWGG 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 320, 608
Bst4CI ACNGT 2 cut(s) 250, 308
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 96, 498
BstDEI CTNAG 2 cut(s) 164, 594
BstDSI CCRYGG 1 cut(s) 107
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 741
BstKTI GATC 6 cut(s) 9, 189, 201, 316, 493, 532
BstMBI GATC 6 cut(s) 6, 186, 198, 313, 490, 529
BstMWI GCNNNNNNNGC 1 cut(s) 583
BstNI CCWGG 2 cut(s) 320, 608
BstNSI RCATGY 2 cut(s) 470, 500
BstSCI CCNGG 2 cut(s) 318, 606
BstV1I GCAGC 2 cut(s) 124, 589
BsuRI GGCC 2 cut(s) 331, 653
BtgI CCRYGG 1 cut(s) 107
BtsCI GGATG 1 cut(s) 741
BtsIMutI CAGTG 1 cut(s) 691
Cac8I GCNNGC 2 cut(s) 96, 498
CaiI CAGNNNCTG 1 cut(s) 747
CciI TCATGA 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 329
CseI GACGC 1 cut(s) 632
CviAII CATG 5 cut(s) 108, 350, 443, 467, 497
DdeI CTNAG 2 cut(s) 164, 594
DpnI GATC 6 cut(s) 8, 188, 200, 315, 492, 531
DpnII GATC 6 cut(s) 6, 186, 198, 313, 490, 529
DrdI GACNNNNNNGTC 1 cut(s) 467
DseDI GACNNNNNNGTC 1 cut(s) 467
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Ecl136II GAGCTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 107
Eco147I AGGCCT 1 cut(s) 653
Eco24I GRGCYC 2 cut(s) 52, 71
Eco53kI GAGCTC 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 69
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 329
EcoRII CCWGG 2 cut(s) 318, 606
EcoT14I CCWWGG 1 cut(s) 107
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 2 cut(s) 52, 71
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 5 cut(s) 111, 353, 446, 470, 500
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FatI CATG 5 cut(s) 107, 349, 442, 466, 496
FbaI TGATCA 1 cut(s) 490
Fnu4HI GCNGC 3 cut(s) 138, 578, 712
FriOI GRGCYC 2 cut(s) 52, 71
Fsp4HI GCNGC 3 cut(s) 138, 578, 712
GluI GCNGC 3 cut(s) 138, 578, 712
HaeIII GGCC 2 cut(s) 331, 653
HapII CCGG 1 cut(s) 634
HgaI GACGC 1 cut(s) 632
Hin1II CATG 5 cut(s) 111, 353, 446, 470, 500
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 6 cut(s) 38, 102, 405, 643, 662, 744
HpaII CCGG 1 cut(s) 634
HphI GGTGA 5 cut(s) 10, 334, 350, 415, 426
Hpy166II GTNNAC 3 cut(s) 253, 619, 700
Hpy188I TCNGA 5 cut(s) 43, 191, 534, 597, 749
Hpy188III TCNNGA 5 cut(s) 28, 302, 359, 443, 741
Hpy8I GTNNAC 3 cut(s) 253, 619, 700
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 5 cut(s) 81, 233, 319, 342, 713
HpyCH4III ACNGT 2 cut(s) 250, 308
HpyCH4V TGCA 3 cut(s) 121, 284, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 583
HpyF3I CTNAG 2 cut(s) 164, 594
Hsp92II CATG 5 cut(s) 111, 353, 446, 470, 500
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 6 cut(s) 6, 186, 198, 313, 490, 529
LmnI GCTCC 2 cut(s) 74, 176
LpnPI CCDG 8 cut(s) 305, 321, 332, 593, 620, 647, 654, 697
Lsp1109I GCAGC 2 cut(s) 124, 589
LweI GCATC 3 cut(s) 108, 271, 293
MaeIII GTNAC 4 cut(s) 142, 388, 395, 432
MalI GATC 6 cut(s) 8, 188, 200, 315, 492, 531
MboI GATC 6 cut(s) 6, 186, 198, 313, 490, 529
MboII GAAGA 2 cut(s) 91, 698
MhlI GDGCHC 2 cut(s) 52, 71
MluCI AATT 3 cut(s) 123, 130, 270
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 7 cut(s) 82, 84, 266, 541, 583, 630, 725
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 2 cut(s) 273, 516
MspI CCGG 1 cut(s) 634
MspR9I CCNGG 2 cut(s) 320, 608
Mva1269I GAATGC 1 cut(s) 218
MvaI CCWGG 2 cut(s) 320, 608
MwoI GCNNNNNNNGC 1 cut(s) 583
NcoI CCATGG 1 cut(s) 107
NdeII GATC 6 cut(s) 6, 186, 198, 313, 490, 529
NlaIII CATG 5 cut(s) 111, 353, 446, 470, 500
NlaIV GGNNCC 2 cut(s) 330, 682
NmuCI GTSAC 3 cut(s) 388, 395, 432
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 2 cut(s) 470, 500
PaeI GCATGC 1 cut(s) 500
PagI TCATGA 1 cut(s) 442
PceI AGGCCT 1 cut(s) 653
PciI ACATGT 1 cut(s) 466
PctI GAATGC 1 cut(s) 218
PfeI GAWTC 4 cut(s) 102, 405, 643, 662
PkrI GCNGC 3 cut(s) 139, 579, 713
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PscI ACATGT 1 cut(s) 466
Psp124BI GAGCTC 1 cut(s) 71
Psp6I CCWGG 2 cut(s) 318, 606
PspGI CCWGG 2 cut(s) 318, 606
PspN4I GGNNCC 2 cut(s) 330, 682
PspPI GGNCC 1 cut(s) 329
PstNI CAGNNNCTG 1 cut(s) 747
SacI GAGCTC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 273, 516
SatI GCNGC 3 cut(s) 138, 578, 712
Sau3AI GATC 6 cut(s) 6, 186, 198, 313, 490, 529
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 2 cut(s) 320, 608
SduI GDGCHC 2 cut(s) 52, 71
SetI ASST 8 cut(s) 71, 181, 258, 279, 439, 556, 705, 732
SfaNI GCATC 3 cut(s) 108, 271, 293
SmlI CTYRAG 2 cut(s) 300, 587
SmoI CTYRAG 2 cut(s) 300, 587
SphI GCATGC 1 cut(s) 500
Sse9I AATT 3 cut(s) 123, 130, 270
SseBI AGGCCT 1 cut(s) 653
SsiI CCGC 1 cut(s) 712
SstI GAGCTC 1 cut(s) 71
StuI AGGCCT 1 cut(s) 653
StyD4I CCNGG 2 cut(s) 318, 606
StyI CCWWGG 1 cut(s) 107
TaaI ACNGT 2 cut(s) 250, 308
TaqI TCGA 1 cut(s) 201
TasI AATT 3 cut(s) 123, 130, 270
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 4 cut(s) 102, 405, 643, 662
Tru1I TTAA 2 cut(s) 273, 516
Tru9I TTAA 2 cut(s) 273, 516
TscAI CASTG 1 cut(s) 691
TseFI GTSAC 3 cut(s) 388, 395, 432
TseI GCWGC 2 cut(s) 137, 577
Tsp45I GTSAC 3 cut(s) 388, 395, 432
TspDTI ATGAA 3 cut(s) 225, 252, 459
TspGWI ACGGA 1 cut(s) 739
TspRI CASTG 1 cut(s) 691
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 1 cut(s) 123
XceI RCATGY 2 cut(s) 470, 500
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.