RchiOBHm_Chr2g0155801

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004137
Physical Location & Seq
Forward (+)
72653105 .. 72655295
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52467

Sequence Viewer

Length: 630 bp
ATGGAGCTCCTGGGATTGTTAATGATGGGTTTTGTGGTCTTGTGCCAAATGAAAGGCTCGGTTGGGTTGAGGTTCGTGATAGATAGAGAAGAGTGCTTCTCTCATGAGGCAAAGTATGAGGGTGACACTGTTCATGTCTCTTTTGTCGTGATCAAAATCGATTCGACTTGGCATTACACTCAAGAAGGAGTTGATCTTGTGGTAAAGGGGCCTTCTGGTGAACAACTTCATGACATACGTGACAAGACGAGTGAGAAGTTTGAATTTGTGGTTCAGAAGGCCGGTGTTCATCACTTCTGCTTCACTAACAAGTCTCCTTACCATGAAACCATTGATTTTGATGTACATTCTGCGCACTTCACATATTATGATGAGCATGCTAAAGATGAGCATTTCAAACCCTTGTTTGAGCAGATTCAAAAGTTGGAGGAAGCCATTTTCAATATCCAATTTGAACAGCACTGGCTAGAGGCTGAGACTGACCGCCAGGCAATATTGAATGAAGGAATGAGTCAGAGGGCAACTCACAAGGCAATGTTTGAATCAGCTGCACTTATTGGAACCAGCCTCCTCCAAGTTTTTCTTTTGAAACGTTTGTTTGAGCGCAAGCTTGGGTCATCTAGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

209

Amino Acids

24.23

Weight (kDa)

5.68

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 29 - 201 9.9e-34 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 354
AciI CCGC 1 cut(s) 484
AclI AACGTT 1 cut(s) 592
AcsI RAATTY 1 cut(s) 263
AfaI GTAC 1 cut(s) 345
AgsI TTSAA 8 cut(s) 263, 397, 419, 442, 455, 499, 542, 589
AjnI CCWGG 2 cut(s) 9, 486
AluBI AGCT 3 cut(s) 7, 548, 610
AluI AGCT 3 cut(s) 7, 548, 610
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 3 cut(s) 142, 318, 470
AoxI GGCC 2 cut(s) 209, 279
ApeKI GCWGC 1 cut(s) 548
ApoI RAATTY 1 cut(s) 263
Asp700I GAANNNNTTC 1 cut(s) 225
AspLEI GCGC 2 cut(s) 355, 606
AspS9I GGNCC 1 cut(s) 209
AsuHPI GGTGA 2 cut(s) 134, 230
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 1 cut(s) 535
BccI CCATC 1 cut(s) 19
BciT130I CCWGG 2 cut(s) 11, 488
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 3 cut(s) 142, 318, 470
BfaI CTAG 3 cut(s) 467, 621, 628
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
Bme1390I CCNGG 2 cut(s) 11, 488
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 210, 562
BmrFI CCNGG 2 cut(s) 11, 488
BplI GAGNNNNNCTC 4 cut(s) 83, 115, 508, 540
BpuEI CTTGAG 1 cut(s) 165
Bsa29I ATCGAT 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 239
BsaBI GATNNNNATC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 10
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 467
Bse3DI GCAATG 1 cut(s) 540
Bse8I GATNNNNATC 1 cut(s) 155
BseBI CCWGG 2 cut(s) 11, 488
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 10
BseJI GATNNNNATC 1 cut(s) 155
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 1 cut(s) 467
BseRI GAGGAG 1 cut(s) 560
BseXI GCAGC 1 cut(s) 535
BsgI GTGCAG 1 cut(s) 534
BshFI GGCC 2 cut(s) 211, 281
BshVI ATCGAT 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 282
BsmAI GTCTC 3 cut(s) 142, 318, 470
BsnI GGCC 2 cut(s) 211, 281
Bsp1286I GDGCHC 1 cut(s) 9
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 2 cut(s) 150, 193
BspACI CCGC 1 cut(s) 484
BspANI GGCC 2 cut(s) 211, 281
BspCNI CTCAG 1 cut(s) 466
BspDI ATCGAT 1 cut(s) 159
BspHI TCATGA 2 cut(s) 103, 229
BspLI GGNNCC 2 cut(s) 210, 562
BsrDI GCAATG 1 cut(s) 540
BsrFI RCCGGY 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 1 cut(s) 467
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 150, 193
Bst2UI CCWGG 2 cut(s) 11, 488
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 1 cut(s) 84
BstAUI TGTACA 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 378, 608
BstDEI CTNAG 1 cut(s) 474
BstHHI GCGC 2 cut(s) 355, 606
BstKTI GATC 2 cut(s) 153, 196
BstMAI GTCTC 3 cut(s) 142, 318, 470
BstMBI GATC 2 cut(s) 150, 193
BstNI CCWGG 2 cut(s) 11, 488
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 9, 486
BstV1I GCAGC 1 cut(s) 535
Bsu15I ATCGAT 1 cut(s) 159
BsuRI GGCC 2 cut(s) 211, 281
BsuTUI ATCGAT 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 126, 460
Cac8I GCNNGC 2 cut(s) 378, 608
CciI TCATGA 2 cut(s) 103, 229
CfoI GCGC 2 cut(s) 355, 606
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 1 cut(s) 344
CviAII CATG 5 cut(s) 104, 134, 230, 323, 377
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 1 cut(s) 474
DpnI GATC 2 cut(s) 152, 195
DpnII GATC 2 cut(s) 150, 193
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 7
Eco24I GRGCYC 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 9, 486
EcoT38I GRGCYC 1 cut(s) 9
FaeI CATG 5 cut(s) 107, 137, 233, 326, 380
FaiI YATR 9 cut(s) 105, 117, 135, 231, 236, 324, 364, 369, 378
FalI AAGNNNNNCTT 2 cut(s) 567, 599
FatI CATG 5 cut(s) 103, 133, 229, 322, 376
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 1 cut(s) 549
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 1 cut(s) 549
FspBI CTAG 3 cut(s) 467, 621, 628
FspI TGCGCA 1 cut(s) 354
GlaI GCGC 2 cut(s) 354, 605
GluI GCNGC 1 cut(s) 549
HaeIII GGCC 2 cut(s) 211, 281
HapII CCGG 1 cut(s) 282
HhaI GCGC 2 cut(s) 355, 606
Hin1II CATG 5 cut(s) 107, 137, 233, 326, 380
Hin6I GCGC 2 cut(s) 353, 604
HinP1I GCGC 2 cut(s) 353, 604
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 5 cut(s) 161, 415, 511, 542, 624
HpaII CCGG 1 cut(s) 282
HphI GGTGA 2 cut(s) 134, 230
Hpy166II GTNNAC 1 cut(s) 221
Hpy188I TCNGA 2 cut(s) 276, 516
Hpy188III TCNNGA 6 cut(s) 76, 104, 148, 182, 230, 621
Hpy8I GTNNAC 1 cut(s) 221
HpyAV CCTTC 4 cut(s) 179, 222, 271, 497
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 238, 592
HpyCH4V TGCA 1 cut(s) 551
HpyF3I CTNAG 1 cut(s) 474
HpySE526I ACGT 2 cut(s) 238, 592
Hsp92II CATG 5 cut(s) 107, 137, 233, 326, 380
HspAI GCGC 2 cut(s) 353, 604
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 2 cut(s) 150, 193
LmnI GCTCC 2 cut(s) 4, 12
LpnPI CCDG 7 cut(s) 23, 201, 295, 448, 473, 500, 577
Lsp1109I GCAGC 1 cut(s) 535
MaeI CTAG 3 cut(s) 467, 621, 628
MaeII ACGT 2 cut(s) 238, 592
MaeIII GTNAC 2 cut(s) 122, 239
MalI GATC 2 cut(s) 152, 195
MboI GATC 2 cut(s) 150, 193
MboII GAAGA 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 2 cut(s) 263, 449
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 1 cut(s) 405
MnlI CCTC 8 cut(s) 63, 100, 112, 421, 463, 510, 578, 581
MroXI GAANNNNTTC 1 cut(s) 225
MseI TTAA 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 11, 488
MvaI CCWGG 2 cut(s) 11, 488
NdeII GATC 2 cut(s) 150, 193
NlaIII CATG 5 cut(s) 107, 137, 233, 326, 380
NlaIV GGNNCC 2 cut(s) 210, 562
NmuCI GTSAC 2 cut(s) 122, 239
NsbI TGCGCA 1 cut(s) 354
NspI RCATGY 1 cut(s) 380
PaeI GCATGC 1 cut(s) 380
PagI TCATGA 2 cut(s) 103, 229
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 3 cut(s) 161, 415, 542
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 1 cut(s) 519
PpsI GAGTC 1 cut(s) 519
Ppu21I YACGTR 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 9
Psp1406I AACGTT 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 9, 486
PspGI CCWGG 2 cut(s) 9, 486
PspN4I GGNNCC 2 cut(s) 210, 562
PspPI GGNCC 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
SacI GAGCTC 1 cut(s) 9
SaqAI TTAA 1 cut(s) 20
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 2 cut(s) 150, 193
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 520
ScrFI CCNGG 2 cut(s) 11, 488
SduI GDGCHC 1 cut(s) 9
SetI ASST 6 cut(s) 9, 74, 241, 550, 595, 612
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
SphI GCATGC 1 cut(s) 380
Sse9I AATT 2 cut(s) 263, 449
SsiI CCGC 1 cut(s) 484
SspI AATATT 1 cut(s) 495
SspMI CTAG 3 cut(s) 467, 621, 628
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 9, 486
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 2 cut(s) 241, 595
TaqI TCGA 2 cut(s) 159, 164
TasI AATT 2 cut(s) 263, 449
TatI WGTACW 1 cut(s) 343
TfiI GAWTC 3 cut(s) 161, 415, 542
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 2 cut(s) 133, 467
TseFI GTSAC 2 cut(s) 122, 239
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 2 cut(s) 122, 239
TspDTI ATGAA 6 cut(s) 65, 122, 218, 278, 339, 516
TspRI CASTG 2 cut(s) 133, 467
XapI RAATTY 1 cut(s) 263
XbaI TCTAGA 1 cut(s) 620
XceI RCATGY 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 3 cut(s) 467, 621, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.