MD09G1128600.v1.1

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004137
Physical Location & Seq
Reverse (-)
9942351 .. 9944392
2042 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1128600.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGGAGACACCATCCATGTCTCTTTTGTGGTAATCAAGGTAGATTCGACTTGGCATTACACCGAAGACGGCGTTGATCTCGTGATAAAGGGACCTGGTGGTGAACAAATTCATGACATACGCGGCAAGACGAGCGAGAAGTATGAGCTAGTGGTTCAGAAGCAAGGTGTTCACCAGTTCTGCTTCACGAACAAGTCTCCTTACCACGAAACCATTGACTTCGACGTACATGCTGCTCACTTCACATACCATGACCAGCATGCTAAAGACGAGCATTTCACACCCTTGCTGGAGCAGATTCAAAAGTTGGAGGAAGCCCTCTATAACATCCAATTCGAACAACATTGGTTAGAAGCTGAGACTGACCGCCAAGCAATAGTGAATGAAGGAATGAGCCGCAGAGCAACACACAAGGCATTGATAGAATCGGCAGCGCTTATCGGAGCTAGCTGCCTCCAAGTTTTTCTTCTGAAACGTTTGTTTGAACGCAAGCTTGGATCTTCTAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

171

Amino Acids

19.54

Weight (kDa)

5.92

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 2 - 162 3.4e-28 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 123
AciI CCGC 3 cut(s) 123, 367, 397
AclI AACGTT 1 cut(s) 475
AclWI GGATC 1 cut(s) 505
AcsI RAATTY 1 cut(s) 108
AfaI GTAC 1 cut(s) 228
AfeI AGCGCT 1 cut(s) 435
AgsI TTSAA 2 cut(s) 302, 485
AjnI CCWGG 1 cut(s) 94
AjuI GAANNNNNNNTTGG 4 cut(s) 450, 477, 482, 509
AluBI AGCT 5 cut(s) 148, 356, 446, 450, 493
AluI AGCT 5 cut(s) 148, 356, 446, 450, 493
Alw26I GTCTC 3 cut(s) 25, 201, 353
AlwI GGATC 1 cut(s) 505
Aor51HI AGCGCT 1 cut(s) 435
ApeKI GCWGC 3 cut(s) 233, 431, 450
ApoI RAATTY 1 cut(s) 108
Asp700I GAANNNNTTC 1 cut(s) 108
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 113, 164
AsuII TTCGAA 1 cut(s) 336
AsuNHI GCTAGC 1 cut(s) 446
AvaII GGWCC 1 cut(s) 92
BauI CACGAG 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 72
BbvI GCAGC 3 cut(s) 220, 437, 443
BccI CCATC 1 cut(s) 20
BceAI ACGGC 1 cut(s) 85
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 1 cut(s) 96
BcoDI GTCTC 3 cut(s) 25, 201, 353
BfaI CTAG 3 cut(s) 149, 447, 504
BfoI RGCGCY 1 cut(s) 437
BisI GCNGC 5 cut(s) 124, 234, 397, 432, 451
BlsI GCNGC 5 cut(s) 125, 235, 398, 433, 452
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 96
BmtI GCTAGC 1 cut(s) 450
BpiI GAAGAC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 311
Bpu14I TTCGAA 1 cut(s) 336
Bse1I ACTGG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 96
BseGI GGATG 2 cut(s) 12, 327
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 3 cut(s) 220, 437, 443
Bsh1236I CGCG 1 cut(s) 123
BslFI GGGAC 1 cut(s) 105
BsmAI GTCTC 3 cut(s) 25, 201, 353
BsmFI GGGAC 1 cut(s) 105
Bsp119I TTCGAA 1 cut(s) 336
Bsp143I GATC 2 cut(s) 76, 497
BspACI CCGC 3 cut(s) 123, 367, 397
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 1 cut(s) 123
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 93
BspOI GCTAGC 1 cut(s) 450
BspPI GGATC 1 cut(s) 505
BspT104I TTCGAA 1 cut(s) 336
BsrI ACTGG 1 cut(s) 175
BssMI GATC 2 cut(s) 76, 497
BssSI CACGAG 1 cut(s) 80
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 261, 448, 491
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 2 cut(s) 12, 327
BstFNI CGCG 1 cut(s) 123
BstH2I RGCGCY 1 cut(s) 437
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 2 cut(s) 79, 500
BstMAI GTCTC 3 cut(s) 25, 201, 353
BstMBI GATC 2 cut(s) 76, 497
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 1 cut(s) 96
BstNSI RCATGY 2 cut(s) 233, 263
BstSCI CCNGG 1 cut(s) 94
BstUI CGCG 1 cut(s) 123
BstV1I GCAGC 3 cut(s) 220, 437, 443
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
BtsCI GGATG 2 cut(s) 12, 327
Cac8I GCNNGC 3 cut(s) 261, 448, 491
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 92
CsiI ACCWGGT 1 cut(s) 94
Csp6I GTAC 1 cut(s) 227
CviAII CATG 5 cut(s) 17, 113, 230, 251, 260
CviJI RGCY 7 cut(s) 148, 317, 356, 396, 446, 450, 493
CviKI_1 RGCY 7 cut(s) 148, 317, 356, 396, 446, 450, 493
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 1 cut(s) 357
DpnI GATC 2 cut(s) 78, 499
DpnII GATC 2 cut(s) 76, 497
Eco47I GGWCC 1 cut(s) 92
Eco47III AGCGCT 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 94
FaeI CATG 5 cut(s) 20, 116, 233, 254, 263
FaiI YATR 9 cut(s) 18, 114, 119, 144, 231, 247, 252, 261, 324
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FaqI GGGAC 1 cut(s) 105
FatI CATG 5 cut(s) 16, 112, 229, 250, 259
Fnu4HI GCNGC 5 cut(s) 124, 234, 397, 432, 451
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 5 cut(s) 124, 234, 397, 432, 451
FspBI CTAG 3 cut(s) 149, 447, 504
GlaI GCGC 1 cut(s) 435
GluI GCNGC 5 cut(s) 124, 234, 397, 432, 451
GsuI CTGGAG 1 cut(s) 311
HaeII RGCGCY 1 cut(s) 437
HhaI GCGC 1 cut(s) 436
Hin1II CATG 5 cut(s) 20, 116, 233, 254, 263
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 3 cut(s) 44, 298, 425
HphI GGTGA 2 cut(s) 113, 164
Hpy166II GTNNAC 2 cut(s) 104, 172
Hpy188I TCNGA 3 cut(s) 159, 443, 471
Hpy188III TCNNGA 4 cut(s) 82, 113, 187, 504
Hpy8I GTNNAC 2 cut(s) 104, 172
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 380
HpyCH4IV ACGT 2 cut(s) 225, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 2 cut(s) 225, 475
Hsp92II CATG 5 cut(s) 20, 116, 233, 254, 263
HspAI GCGC 1 cut(s) 434
Kzo9I GATC 2 cut(s) 76, 497
LmnI GCTCC 2 cut(s) 292, 443
LpnPI CCDG 5 cut(s) 81, 108, 188, 269, 275
Lsp1109I GCAGC 3 cut(s) 220, 437, 443
MabI ACCWGGT 1 cut(s) 94
MaeI CTAG 3 cut(s) 149, 447, 504
MaeII ACGT 2 cut(s) 225, 475
MalI GATC 2 cut(s) 78, 499
MboI GATC 2 cut(s) 76, 497
MboII GAAGA 3 cut(s) 77, 458, 492
MflI RGATCY 1 cut(s) 497
MluCI AATT 2 cut(s) 108, 332
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 3 cut(s) 304, 329, 464
MroXI GAANNNNTTC 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 96
MvaI CCWGG 1 cut(s) 96
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeII GATC 2 cut(s) 76, 497
NheI GCTAGC 1 cut(s) 446
NlaIII CATG 5 cut(s) 20, 116, 233, 254, 263
NlaIV GGNNCC 1 cut(s) 93
NspI RCATGY 2 cut(s) 233, 263
NspV TTCGAA 1 cut(s) 336
PaeI GCATGC 1 cut(s) 263
PagI TCATGA 1 cut(s) 112
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 3 cut(s) 44, 298, 425
PkrI GCNGC 5 cut(s) 125, 235, 398, 433, 452
PpuMI RGGWCCY 1 cut(s) 92
Psp1406I AACGTT 1 cut(s) 475
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 92
PspPPI RGGWCCY 1 cut(s) 92
PsuI RGATCY 1 cut(s) 497
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
SatI GCNGC 5 cut(s) 124, 234, 397, 432, 451
Sau3AI GATC 2 cut(s) 76, 497
Sau96I GGNCC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 96
SexAI ACCWGGT 1 cut(s) 94
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 1 cut(s) 92
SphI GCATGC 1 cut(s) 263
Sse9I AATT 2 cut(s) 108, 332
SsiI CCGC 3 cut(s) 123, 367, 397
SspMI CTAG 3 cut(s) 149, 447, 504
StyD4I CCNGG 1 cut(s) 94
TaiI ACGT 2 cut(s) 228, 478
TaqI TCGA 3 cut(s) 47, 222, 336
TasI AATT 2 cut(s) 108, 332
TauI GCSGC 2 cut(s) 126, 399
TfiI GAWTC 3 cut(s) 44, 298, 425
TseI GCWGC 3 cut(s) 233, 431, 450
TspDTI ATGAA 2 cut(s) 101, 399
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 1 cut(s) 108
XbaI TCTAGA 1 cut(s) 503
XceI RCATGY 2 cut(s) 233, 263
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 3 cut(s) 149, 447, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.