Rh2DG536100

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004137
Physical Location & Seq
Forward (+)
76617828 .. 76619236
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG536100.1

Sequence Viewer

Length: 582 bp
ATGAAAGGCTCCGTTGGGTTGAGGTTCGTGATAGATAGAGAAGAGTGCTTCTCTCATGAGGCAAAGTATGAGGGTGACACTGTTCATGTCTCTTTTGTCGTGATCAAAATCGATTCGACTTGGCATTACACTCAAGAAGGAGTTGATCTTGTGGTAAAGGGGCCTTCTGGTGAACAACTTCATGACATACGTGACAAGACGAGTGAGAAGTTTGAATTTGTGGTTCAGAAGGCAGGTGTTCATCACTTCTGCTTCACTAACAAATCTCCTTACCATGAAACCATTGATTTTGATGTACATTCTGCGCACTTCACATACTATGACGAGCATGCCAAAGATGAGCATTTCAAACCCTTGTTTGAGCAGATTCAAAAGTTGGAGGAAGCCATTTTCAATATCCAATTTGAACAACACTGGCTAGAGGCTGAGACTGACCGCCAGGCAATATTGAATGAAGGAATGAGTCAGAGGGCAACTCACAAGGCAATGTTTGAATCAGCTGCACTTATTGGAACCAGCCTCCTCCAAGTTTTTCTTTTGAAACGTTTGTTTGAGCGCAAGCTTGGGTCATCTAGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

193

Amino Acids

22.45

Weight (kDa)

5.78

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 13 - 185 7.4e-34 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
Acc16I TGCGCA 1 cut(s) 306
Acc36I ACCTGC 1 cut(s) 224
AciI CCGC 1 cut(s) 436
AclI AACGTT 1 cut(s) 544
AcsI RAATTY 1 cut(s) 215
AfaI GTAC 1 cut(s) 297
AgsI TTSAA 8 cut(s) 215, 349, 371, 394, 407, 451, 494, 541
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 2 cut(s) 500, 562
AluI AGCT 2 cut(s) 500, 562
Alw26I GTCTC 2 cut(s) 94, 422
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 1 cut(s) 215
Asp700I GAANNNNTTC 1 cut(s) 177
AspLEI GCGC 2 cut(s) 307, 558
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 86, 182
BbvI GCAGC 1 cut(s) 487
BciT130I CCWGG 1 cut(s) 440
BclI TGATCA 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 94, 422
BfaI CTAG 3 cut(s) 419, 573, 580
BfuAI ACCTGC 1 cut(s) 224
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 3 cut(s) 10, 162, 514
BmrFI CCNGG 1 cut(s) 440
BplI GAGNNNNNCTC 4 cut(s) 35, 67, 460, 492
BpuEI CTTGAG 1 cut(s) 117
Bsa29I ATCGAT 1 cut(s) 111
BsaAI YACGTR 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 419
Bse3DI GCAATG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 107
BseBI CCWGG 1 cut(s) 440
BseCI ATCGAT 1 cut(s) 111
BseJI GATNNNNATC 1 cut(s) 107
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 419
BseRI GAGGAG 1 cut(s) 512
BseXI GCAGC 1 cut(s) 487
BsgI GTGCAG 1 cut(s) 486
BshFI GGCC 1 cut(s) 163
BshVI ATCGAT 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 94, 422
BsnI GGCC 1 cut(s) 163
Bsp1407I TGTACA 1 cut(s) 295
Bsp143I GATC 2 cut(s) 102, 145
BspACI CCGC 1 cut(s) 436
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 1 cut(s) 418
BspDI ATCGAT 1 cut(s) 111
BspHI TCATGA 2 cut(s) 55, 181
BspLI GGNNCC 3 cut(s) 10, 162, 514
BspMI ACCTGC 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 492
BsrGI TGTACA 1 cut(s) 295
BsrI ACTGG 1 cut(s) 419
BssMI GATC 2 cut(s) 102, 145
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 1 cut(s) 36
BstAUI TGTACA 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 330, 560
BstDEI CTNAG 1 cut(s) 426
BstHHI GCGC 2 cut(s) 307, 558
BstKTI GATC 2 cut(s) 105, 148
BstMAI GTCTC 2 cut(s) 94, 422
BstMBI GATC 2 cut(s) 102, 145
BstNI CCWGG 1 cut(s) 440
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 438
BstV1I GCAGC 1 cut(s) 487
Bsu15I ATCGAT 1 cut(s) 111
BsuRI GGCC 1 cut(s) 163
BsuTUI ATCGAT 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 78, 412
BveI ACCTGC 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 330, 560
CciI TCATGA 2 cut(s) 55, 181
CfoI GCGC 2 cut(s) 307, 558
Cfr13I GGNCC 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 111
Csp6I GTAC 1 cut(s) 296
CviAII CATG 5 cut(s) 56, 86, 182, 275, 329
CviJI RGCY 8 cut(s) 9, 163, 386, 418, 425, 500, 519, 562
CviKI_1 RGCY 8 cut(s) 9, 163, 386, 418, 425, 500, 519, 562
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 1 cut(s) 426
DpnI GATC 2 cut(s) 104, 147
DpnII GATC 2 cut(s) 102, 145
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EcoO109I RGGNCCY 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 438
FaeI CATG 5 cut(s) 59, 89, 185, 278, 332
FaiI YATR 9 cut(s) 57, 69, 87, 183, 188, 276, 316, 321, 330
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FatI CATG 5 cut(s) 55, 85, 181, 274, 328
FbaI TGATCA 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 501
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 3 cut(s) 419, 573, 580
FspI TGCGCA 1 cut(s) 306
GlaI GCGC 2 cut(s) 306, 557
GluI GCNGC 1 cut(s) 501
HaeIII GGCC 1 cut(s) 163
HhaI GCGC 2 cut(s) 307, 558
Hin1II CATG 5 cut(s) 59, 89, 185, 278, 332
Hin6I GCGC 2 cut(s) 305, 556
HinP1I GCGC 2 cut(s) 305, 556
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 5 cut(s) 113, 367, 463, 494, 576
HphI GGTGA 2 cut(s) 86, 182
Hpy166II GTNNAC 1 cut(s) 173
Hpy188I TCNGA 2 cut(s) 228, 468
Hpy188III TCNNGA 6 cut(s) 28, 56, 100, 134, 182, 573
Hpy8I GTNNAC 1 cut(s) 173
HpyAV CCTTC 4 cut(s) 131, 174, 223, 449
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 2 cut(s) 190, 544
HpyCH4V TGCA 1 cut(s) 503
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 2 cut(s) 190, 544
Hsp92II CATG 5 cut(s) 59, 89, 185, 278, 332
HspAI GCGC 2 cut(s) 305, 556
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 2 cut(s) 102, 145
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 6 cut(s) 153, 219, 400, 425, 452, 529
Lsp1109I GCAGC 1 cut(s) 487
MaeI CTAG 3 cut(s) 419, 573, 580
MaeII ACGT 2 cut(s) 190, 544
MaeIII GTNAC 2 cut(s) 74, 191
MalI GATC 2 cut(s) 104, 147
MboI GATC 2 cut(s) 102, 145
MboII GAAGA 1 cut(s) 53
MluCI AATT 2 cut(s) 215, 401
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 8 cut(s) 15, 52, 64, 373, 415, 462, 530, 533
MroXI GAANNNNTTC 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 440
MvaI CCWGG 1 cut(s) 440
NdeII GATC 2 cut(s) 102, 145
NlaIII CATG 5 cut(s) 59, 89, 185, 278, 332
NlaIV GGNNCC 3 cut(s) 10, 162, 514
NmuCI GTSAC 2 cut(s) 74, 191
NsbI TGCGCA 1 cut(s) 306
NspI RCATGY 1 cut(s) 332
PaeI GCATGC 1 cut(s) 332
PagI TCATGA 2 cut(s) 55, 181
PaqCI CACCTGC 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 3 cut(s) 113, 367, 494
PkrI GCNGC 1 cut(s) 502
PleI GAGTC 1 cut(s) 471
PpsI GAGTC 1 cut(s) 471
Ppu21I YACGTR 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 544
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 3 cut(s) 10, 162, 514
PspPI GGNCC 1 cut(s) 161
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 2 cut(s) 102, 145
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 1 cut(s) 440
SetI ASST 6 cut(s) 26, 193, 238, 502, 547, 564
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
SphI GCATGC 1 cut(s) 332
Sse9I AATT 2 cut(s) 215, 401
SsiI CCGC 1 cut(s) 436
SspI AATATT 1 cut(s) 447
SspMI CTAG 3 cut(s) 419, 573, 580
StyD4I CCNGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 2 cut(s) 193, 547
TaqI TCGA 2 cut(s) 111, 116
TasI AATT 2 cut(s) 215, 401
TatI WGTACW 1 cut(s) 295
TfiI GAWTC 3 cut(s) 113, 367, 494
TscAI CASTG 2 cut(s) 85, 419
TseFI GTSAC 2 cut(s) 74, 191
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 2 cut(s) 74, 191
TspDTI ATGAA 6 cut(s) 17, 74, 170, 230, 291, 468
TspRI CASTG 2 cut(s) 85, 419
XapI RAATTY 1 cut(s) 215
XbaI TCTAGA 1 cut(s) 572
XceI RCATGY 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 3 cut(s) 419, 573, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.