AT3G15750

Essential protein Yae1, N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002936
Physical Location & Seq
Forward (+)
5334844 .. 5336485
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G15750.1

Sequence Viewer

Length: 600 bp
ATGGAGCCGCATGAAGATGTGAAGGATAATTTGGCCAAAGAGCTTTATGGAGAAAGTGTACAGCTTTCGAAGCATGATGATCCCTTTTACGGAAGTTCAGATGAAGAAGACTATAGTGAAGCTCGCGTGTTGGGCAATGAGAATAAGCAGAGACGTGTGAAATTCCACTCGGCTGGATATCGTGATGGGATTGTTGCCGGGAAAGAAGCTATTGCGCAGGAAGGTTATAACTTTGGCTATAAGGAATCAGTTCTTAATGGTTACCAGTTTGGTCTTGTTAGAGGTGTTTCCAGTGCGTTGGCTTTCCTCCCGGATGAGCTTAGAGAAAAGCTTATCGATAAACAAGAAACTAGAGAGAAGTTTCAGAAATTACACAGTTCTGTGCATGCTCTTTCGACGGAAGTCGCAATGAAGCGGTTCTATGAAACTTTGACTACGAAGCAAGGGGAAGAGAAGAGTGGAGAAGAAGGGCCCGACTCTTGTTCTGATTCAGGTTCTGGTTCAGGTGTTAATGTCACAACCGACTTAGGAAGCTATGTTACTGAGCTAAGTTCTCTTCTTGACAAATCTCCAACGATTGAAGTTAAATTTGACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yae1_N

Protein Analysis

199

Amino Acids

22.09

Weight (kDa)

4.86

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yae1_N PF09811 59 - 96 1.1e-10 Essential protein Yae1, N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
Acc16I TGCGCA 1 cut(s) 216
AccII CGCG 1 cut(s) 126
AciI CCGC 2 cut(s) 8, 415
AclWI GGATC 1 cut(s) 74
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 2 cut(s) 161, 587
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 89
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 1 cut(s) 581
AjiI CACGTC 1 cut(s) 155
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 8 cut(s) 43, 64, 122, 209, 319, 331, 534, 547
AluI AGCT 8 cut(s) 43, 64, 122, 209, 319, 331, 534, 547
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 2 cut(s) 33, 470
ApaI GGGCCC 1 cut(s) 474
ApoI RAATTY 2 cut(s) 161, 587
Asp700I GAANNNNTTC 2 cut(s) 249, 416
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 470, 471
AsuC2I CCSGG 2 cut(s) 199, 311
AsuII TTCGAA 1 cut(s) 68
BaeGI GKGCMC 1 cut(s) 474
BalI TGGCCA 1 cut(s) 35
BanII GRGCYC 1 cut(s) 474
BarI GAAGNNNNNNTAC 2 cut(s) 523, 555
BbsI GAAGAC 1 cut(s) 114
BccI CCATC 1 cut(s) 179
BcnI CCSGG 2 cut(s) 199, 311
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 112
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
Bme1390I CCNGG 2 cut(s) 199, 311
BmgBI CACGTC 1 cut(s) 155
BmgT120I GGNCC 2 cut(s) 470, 471
BmiI GGNNCC 2 cut(s) 6, 472
BmrFI CCNGG 2 cut(s) 199, 311
BoxI GACNNNNGTC 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 114
Bpu14I TTCGAA 1 cut(s) 68
BpuMI CCSGG 2 cut(s) 199, 311
Bsa29I ATCGAT 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse1I ACTGG 2 cut(s) 265, 291
Bse3DI GCAATG 2 cut(s) 142, 414
BseCI ATCGAT 1 cut(s) 336
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMI GCAATG 2 cut(s) 142, 414
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 265, 291
BseSI GKGCMC 1 cut(s) 474
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 2 cut(s) 35, 472
BshVI ATCGAT 1 cut(s) 336
BsiSI CCGG 2 cut(s) 198, 311
BslI CCNNNNNNNGG 1 cut(s) 89
BsmAI GTCTC 1 cut(s) 145
BsmBI CGTCTC 1 cut(s) 145
BsnI GGCC 2 cut(s) 35, 472
Bsp119I TTCGAA 1 cut(s) 68
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 474
Bsp1407I TGTACA 1 cut(s) 58
Bsp143I GATC 1 cut(s) 79
BspACI CCGC 2 cut(s) 8, 415
BspANI GGCC 2 cut(s) 35, 472
BspCNI CTCAG 1 cut(s) 535
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 2 cut(s) 6, 472
BspPI GGATC 1 cut(s) 74
BspT104I TTCGAA 1 cut(s) 68
BsrDI GCAATG 2 cut(s) 142, 414
BsrGI TGTACA 1 cut(s) 58
BsrI ACTGG 2 cut(s) 265, 291
BssMI GATC 1 cut(s) 79
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 3 cut(s) 444, 449, 561
BstAUI TGTACA 1 cut(s) 58
BstBI TTCGAA 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 124, 387
BstDEI CTNAG 4 cut(s) 320, 526, 543, 548
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 1 cut(s) 319
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 1 cut(s) 82
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 2 cut(s) 70, 132
BstNSI RCATGY 1 cut(s) 389
BstPAI GACNNNNGTC 1 cut(s) 401
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 197, 309
BstSFI CTRYAG 1 cut(s) 112
BstSLI GKGCMC 1 cut(s) 474
BstUI CGCG 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 114
BstXI CCANNNNNNTGG 2 cut(s) 173, 298
Bsu15I ATCGAT 1 cut(s) 336
BsuRI GGCC 2 cut(s) 35, 472
BsuTUI ATCGAT 1 cut(s) 336
BtrI CACGTC 1 cut(s) 155
BtsCI GGATG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 298
Cac8I GCNNGC 2 cut(s) 124, 387
CaiI CAGNNNCTG 1 cut(s) 497
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 470, 471
ClaI ATCGAT 1 cut(s) 336
Csp6I GTAC 1 cut(s) 59
CviAII CATG 3 cut(s) 11, 74, 386
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 4 cut(s) 320, 526, 543, 548
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 3 cut(s) 444, 449, 561
EarI CTCTTC 3 cut(s) 444, 449, 561
Eco24I GRGCYC 1 cut(s) 474
Eco32I GATATC 1 cut(s) 179
Eco91I GGTNACC 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 470
EcoO65I GGTNACC 1 cut(s) 260
EcoRV GATATC 1 cut(s) 179
EcoT38I GRGCYC 1 cut(s) 474
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 3 cut(s) 14, 77, 389
FatI CATG 3 cut(s) 10, 73, 385
Fnu4HI GCNGC 1 cut(s) 8
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 1 cut(s) 8
FspBI CTAG 1 cut(s) 351
FspI TGCGCA 1 cut(s) 216
GlaI GCGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 8
HaeIII GGCC 2 cut(s) 35, 472
HapII CCGG 2 cut(s) 198, 311
HhaI GCGC 1 cut(s) 217
Hin1II CATG 3 cut(s) 14, 77, 389
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 3 cut(s) 245, 476, 488
HpaII CCGG 2 cut(s) 198, 311
Hpy166II GTNNAC 1 cut(s) 59
Hpy188I TCNGA 3 cut(s) 100, 366, 487
Hpy188III TCNNGA 2 cut(s) 182, 560
Hpy8I GTNNAC 1 cut(s) 59
Hpy99I CGWCG 1 cut(s) 400
HpyAV CCTTC 3 cut(s) 16, 215, 461
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 132
HpyF3I CTNAG 4 cut(s) 320, 526, 543, 548
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 3 cut(s) 14, 77, 389
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 1 cut(s) 79
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 159, 203, 211, 278, 304, 324, 477, 483, 489
MaeI CTAG 1 cut(s) 351
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 3 cut(s) 260, 514, 538
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 7 cut(s) 26, 116, 119, 461, 466, 476, 548
MhlI GDGCHC 1 cut(s) 474
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 4 cut(s) 28, 161, 368, 587
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 596
MnlI CCTC 2 cut(s) 275, 317
Mox20I TGGCCA 1 cut(s) 35
MroXI GAANNNNTTC 2 cut(s) 249, 416
MscI TGGCCA 1 cut(s) 35
MseI TTAA 3 cut(s) 255, 510, 585
MslI CAYNNNNRTG 1 cut(s) 15
Msp20I TGGCCA 1 cut(s) 35
MspI CCGG 2 cut(s) 198, 311
MspR9I CCNGG 2 cut(s) 199, 311
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 70, 132
NciI CCSGG 2 cut(s) 199, 311
NdeII GATC 1 cut(s) 79
NlaIII CATG 3 cut(s) 14, 77, 389
NlaIV GGNNCC 2 cut(s) 6, 472
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 1 cut(s) 514
NsbI TGCGCA 1 cut(s) 216
NspI RCATGY 1 cut(s) 389
NspV TTCGAA 1 cut(s) 68
PaeI GCATGC 1 cut(s) 389
PdmI GAANNNNTTC 2 cut(s) 249, 416
PfeI GAWTC 2 cut(s) 245, 488
PfoI TCCNGGA 1 cut(s) 309
PkrI GCNGC 1 cut(s) 9
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
PshAI GACNNNNGTC 1 cut(s) 401
PsiI TTATAA 1 cut(s) 228
PspEI GGTNACC 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 6, 472
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 2 cut(s) 470, 471
PstNI CAGNNNCTG 1 cut(s) 497
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 3 cut(s) 255, 510, 585
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 1 cut(s) 79
Sau96I GGNCC 2 cut(s) 470, 471
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 199, 311
SduI GDGCHC 1 cut(s) 474
SfcI CTRYAG 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 15
SphI GCATGC 1 cut(s) 389
Sse9I AATT 4 cut(s) 28, 161, 368, 587
SsiI CCGC 2 cut(s) 8, 415
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 2 cut(s) 197, 309
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 1 cut(s) 157
TaqI TCGA 3 cut(s) 68, 336, 395
TasI AATT 4 cut(s) 28, 161, 368, 587
TatI WGTACW 1 cut(s) 58
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 2 cut(s) 245, 488
Tru1I TTAA 3 cut(s) 255, 510, 585
Tru9I TTAA 3 cut(s) 255, 510, 585
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 1 cut(s) 514
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 4 cut(s) 27, 117, 425, 438
TspGWI ACGGA 2 cut(s) 105, 413
TspRI CASTG 1 cut(s) 298
XapI RAATTY 2 cut(s) 161, 587
XceI RCATGY 1 cut(s) 389
XmnI GAANNNNTTC 2 cut(s) 249, 416
XspI CTAG 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.