MD02G1294600.v1.1

Essential protein Yae1, N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002936
Physical Location & Seq
Reverse (-)
34944681 .. 34947162
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1294600.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGTCCAAAACCTGTTGGCGTTAACAAAGTGTTGTAACCTCTGCAGCTTCTCCTCCTCTCCATTTTTTTATTTTTCTCCAGGGGAGATGGAGGGAAGTTTTGCTCATGAGCTCTACTCAGAAAGTTTAAGCCTAGAAAAAATACAATTGGATCCTACACCCAGTGCGAACAGTATAGAAAGTGATCTACACGACCATGATAGAGTTGAGCCATGTGATGAAGATGCATCTTGGTGTGCTTCTGACGATGAATTGCATGAACAATCTGCTTTGGGCAGGGAATGGCAGAGGAGACATAACCAATACCATACGACTGGTTACCGTGATGGTGTAATAGCGGGAAAAGAAGCTTCTTCACAAGAGGGATTTAATATTGGCTTTAAGAAATCGGTCCTTGTTGGGTACAACTGGGGTCTTGTCAGAGGTGTTACTAGTGCGCTGGCTTGCCTTCCAGATGGTTTAAGGGAGAAGTTGATTGAAACAGAAGAACAAAGAAATGGATTTCAAAGATTGTTTGAATCTGTGCATTCTATTTCAACGAAAGATGCCCTCGGGTATTTGTACGATGATATGATTGCGAAAAAAGATAGAGAACAGAGTGAAATCTCTGATACTACTAGTTCTCTTGAAGTTGGTTCGCCAGAGAAAATATCACATGTTAGTGGCCTAGGCAATCACCTTGCAGAACTCCGGTCACTTCTTGTCAAGTGTCCTGCAGTCAACGTACATCTACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yae1_N

Protein Analysis

246

Amino Acids

27.26

Weight (kDa)

4.98

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yae1_N PF09811 106 - 143 3.5e-10 Essential protein Yae1, N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 338
AclWI GGATC 2 cut(s) 146, 159
AdeI CACNNNGTG 1 cut(s) 164
AfaI GTAC 3 cut(s) 404, 563, 726
AflIII ACRYGT 1 cut(s) 655
AgsI TTSAA 5 cut(s) 479, 506, 518, 537, 629
AhlI ACTAGT 2 cut(s) 431, 617
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 3 cut(s) 48, 112, 350
AluI AGCT 3 cut(s) 48, 112, 350
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 286
AlwI GGATC 2 cut(s) 146, 159
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 1 cut(s) 664
ApeKI GCWGC 1 cut(s) 45
AspA2I CCTAGG 1 cut(s) 667
AspLEI GCGC 1 cut(s) 439
AspS9I GGNCC 2 cut(s) 4, 391
AsuHPI GGTGA 1 cut(s) 668
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 4, 391
AvrII CCTAGG 1 cut(s) 667
BamHI GGATCC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 1 cut(s) 57
BccI CCATC 3 cut(s) 82, 320, 449
BciT130I CCWGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 286
BcuI ACTAGT 2 cut(s) 431, 617
BfaI CTAG 4 cut(s) 134, 432, 618, 668
BfmI CTRYAG 2 cut(s) 43, 714
BisI GCNGC 1 cut(s) 46
BlnI CCTAGG 1 cut(s) 667
BlsI GCNGC 1 cut(s) 47
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 2 cut(s) 4, 391
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 2 cut(s) 4, 391
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 1 cut(s) 81
BmrI ACTGGG 2 cut(s) 156, 418
BmsI GCATC 3 cut(s) 214, 236, 535
BmuI ACTGGG 2 cut(s) 156, 418
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 80, 550, 667
BsaWI WCCGGW 1 cut(s) 690
Bse1I ACTGG 3 cut(s) 162, 319, 413
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 80, 550, 667
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 3 cut(s) 162, 319, 413
BseRI GAGGAG 3 cut(s) 43, 46, 304
BseXI GCAGC 1 cut(s) 57
BshFI GGCC 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 1 cut(s) 691
BsmAI GTCTC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 1 cut(s) 666
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 2 cut(s) 151, 184
BspACI CCGC 1 cut(s) 338
BspANI GGCC 1 cut(s) 666
BspCNI CTCAG 1 cut(s) 131
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 153
BspMAI CTGCAG 2 cut(s) 47, 718
BspPI GGATC 2 cut(s) 146, 159
BsrI ACTGG 3 cut(s) 162, 319, 413
BssECI CCNNGG 3 cut(s) 80, 550, 667
BssMI GATC 2 cut(s) 151, 184
BssT1I CCWWGG 1 cut(s) 667
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 2 cut(s) 173, 323
BstC8I GCNNGC 2 cut(s) 441, 445
BstDEI CTNAG 1 cut(s) 118
BstEII GGTNACC 1 cut(s) 317
BstHHI GCGC 1 cut(s) 439
BstKTI GATC 2 cut(s) 154, 187
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 2 cut(s) 151, 184
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 659
BstPI GGTNACC 1 cut(s) 317
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 2 cut(s) 43, 714
BstV1I GCAGC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 314
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 1 cut(s) 666
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 441, 445
CciI TCATGA 1 cut(s) 106
CfoI GCGC 1 cut(s) 439
Cfr13I GGNCC 2 cut(s) 4, 391
Csp6I GTAC 3 cut(s) 403, 562, 725
CviAII CATG 5 cut(s) 107, 197, 213, 257, 656
CviJI RGCY 8 cut(s) 48, 112, 132, 211, 350, 378, 443, 666
CviKI_1 RGCY 8 cut(s) 48, 112, 132, 211, 350, 378, 443, 666
CviQI GTAC 3 cut(s) 403, 562, 725
DdeI CTNAG 1 cut(s) 118
DpnI GATC 2 cut(s) 153, 186
DpnII GATC 2 cut(s) 151, 184
DraIII CACNNNGTG 1 cut(s) 164
Ecl136II GAGCTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 4, 391
Eco53kI GAGCTC 1 cut(s) 112
Eco88I CYCGRG 1 cut(s) 551
Eco91I GGTNACC 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 112
EcoO65I GGTNACC 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 667
EcoT22I ATGCAT 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 667
FaeI CATG 5 cut(s) 110, 200, 216, 260, 659
FatI CATG 5 cut(s) 106, 196, 212, 256, 655
FauI CCCGC 1 cut(s) 331
Fnu4HI GCNGC 1 cut(s) 46
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 1 cut(s) 46
FspBI CTAG 4 cut(s) 134, 432, 618, 668
GlaI GCGC 1 cut(s) 438
GluI GCNGC 1 cut(s) 46
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 666
HapII CCGG 1 cut(s) 691
HhaI GCGC 1 cut(s) 439
Hin1II CATG 5 cut(s) 110, 200, 216, 260, 659
Hin6I GCGC 1 cut(s) 437
HinP1I GCGC 1 cut(s) 437
HincII GTYRAC 2 cut(s) 24, 721
HindII GTYRAC 2 cut(s) 24, 721
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 1 cut(s) 518
HpaI GTTAAC 1 cut(s) 24
HpaII CCGG 1 cut(s) 691
HphI GGTGA 1 cut(s) 668
Hpy166II GTNNAC 2 cut(s) 24, 721
Hpy188I TCNGA 4 cut(s) 121, 244, 422, 610
Hpy188III TCNNGA 3 cut(s) 107, 452, 626
Hpy8I GTNNAC 2 cut(s) 24, 721
HpyAV CCTTC 1 cut(s) 458
HpyCH4III ACNGT 2 cut(s) 173, 323
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 6 cut(s) 45, 227, 256, 526, 683, 716
HpyF3I CTNAG 1 cut(s) 118
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 5 cut(s) 110, 200, 216, 260, 659
HspAI GCGC 1 cut(s) 437
KspAI GTTAAC 1 cut(s) 24
Kzo9I GATC 2 cut(s) 151, 184
Lsp1109I GCAGC 1 cut(s) 57
LweI GCATC 3 cut(s) 214, 236, 535
MaeI CTAG 4 cut(s) 134, 432, 618, 668
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 4 cut(s) 35, 317, 427, 693
MalI GATC 2 cut(s) 153, 186
MboI GATC 2 cut(s) 151, 184
MboII GAAGA 3 cut(s) 233, 345, 497
MfeI CAATTG 1 cut(s) 146
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 2 cut(s) 146, 251
MnlI CCTC 8 cut(s) 50, 64, 67, 85, 282, 355, 416, 560
Mph1103I ATGCAT 1 cut(s) 229
MseI TTAA 5 cut(s) 23, 128, 369, 381, 461
MslI CAYNNNNRTG 3 cut(s) 195, 232, 660
MspI CCGG 1 cut(s) 691
MspR9I CCNGG 1 cut(s) 81
MunI CAATTG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 1 cut(s) 81
NdeII GATC 2 cut(s) 151, 184
NlaIII CATG 5 cut(s) 110, 200, 216, 260, 659
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 693
NsiI ATGCAT 1 cut(s) 229
NspI RCATGY 1 cut(s) 659
PagI TCATGA 1 cut(s) 106
PciI ACATGT 1 cut(s) 655
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 1 cut(s) 518
PkrI GCNGC 1 cut(s) 47
PscI ACATGT 1 cut(s) 655
Psp124BI GAGCTC 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 79
PspEI GGTNACC 1 cut(s) 317
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 4, 391
PstI CTGCAG 2 cut(s) 47, 718
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 3 cut(s) 404, 563, 726
RsaNI GTAC 3 cut(s) 403, 562, 725
RseI CAYNNNNRTG 3 cut(s) 195, 232, 660
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 5 cut(s) 23, 128, 369, 381, 461
SatI GCNGC 1 cut(s) 46
Sau3AI GATC 2 cut(s) 151, 184
Sau96I GGNCC 2 cut(s) 4, 391
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 1 cut(s) 114
SetI ASST 8 cut(s) 15, 42, 50, 114, 352, 427, 681, 726
SfaNI GCATC 3 cut(s) 214, 236, 535
SfcI CTRYAG 2 cut(s) 43, 714
SinI GGWCC 2 cut(s) 4, 391
SmiMI CAYNNNNRTG 3 cut(s) 195, 232, 660
SpeI ACTAGT 2 cut(s) 431, 617
Sse9I AATT 2 cut(s) 146, 251
SsiI CCGC 1 cut(s) 338
SspI AATATT 1 cut(s) 373
SspMI CTAG 4 cut(s) 134, 432, 618, 668
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 1 cut(s) 79
StyI CCWWGG 1 cut(s) 667
TaaI ACNGT 2 cut(s) 173, 323
TaiI ACGT 1 cut(s) 726
TaqII GACCGA 1 cut(s) 379
TasI AATT 2 cut(s) 146, 251
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 5 cut(s) 23, 128, 369, 381, 461
Tru9I TTAA 5 cut(s) 23, 128, 369, 381, 461
TscAI CASTG 1 cut(s) 169
TseFI GTSAC 1 cut(s) 693
TseI GCWGC 1 cut(s) 45
Tsp45I GTSAC 1 cut(s) 693
TspDTI ATGAA 3 cut(s) 234, 264, 273
TspRI CASTG 1 cut(s) 169
VpaK11BI GGWCC 2 cut(s) 4, 391
XceI RCATGY 1 cut(s) 659
XmaJI CCTAGG 1 cut(s) 667
XspI CTAG 4 cut(s) 134, 432, 618, 668
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.