Rroxscaffold_4G00328180

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002936
Physical Location & Seq
Reverse (-)
61077907 .. 61080224
2318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328180.1

Sequence Viewer

Length: 636 bp
ATGGAGGGTAGTTTTGCTGAGGAGCTTTACTCAGAAAGTTTACAGCTATCAAAAATGCAATTGGGTTCCACATCGAACGTCACCGATCATGGTAAGGTTCAGTTGTCGAATGAAGATGGATCTTCATGGTGTGGTTCTGATGACGAAGTGAATGAAGCCTCTGATATGGACAAGGAATGGCAGAGGAGACGTGACCAATTCCATACGATGGGATACCGTGATGGTGTTATAGCTGGAAAGGAAACTTCTTCTCAGGAAGGATTCAATATTGGCTTTAAGCAATCAGTCCATGTTGGTTACAACTGGGGTCTTGTGAGAGGTGTTACGAGCACTTTGGCTTCCCTTCCAGATGGCATAAGGGAAAAGTTGATTGAAACAGAAGAACAAAGAACTCAATTTCAGGGATTGTATGAATCTGTTCATTCCCTTTCTACAACAGATGCCCTTGGGTTGTTTAACGATGAAATCACAAGTAAAAATGATAGAGAACAAATTGAAAGTTCAGAGACTAGTTCTTTTGAAGTTGAAACTGGTTTGGAAGAGCAAAGATTAGACCGTAGTGGTCTAGGAAATTACTCTGCAAAGCTCCAATCACTTCTTTTGGAGTCTCCTGGAATCAAAGTAAAGTTCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

211

Amino Acids

23.52

Weight (kDa)

4.59

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yae1_N PF09811 71 - 108 7.3e-10 Essential protein Yae1, N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 208
AclWI GGATC 1 cut(s) 127
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 5 cut(s) 265, 374, 497, 521, 527
AhlI ACTAGT 1 cut(s) 509
AjiI CACGTC 1 cut(s) 191
AjnI CCWGG 1 cut(s) 610
AluBI AGCT 4 cut(s) 25, 46, 233, 586
AluI AGCT 4 cut(s) 25, 46, 233, 586
Alw21I GWGCWC 1 cut(s) 332
Alw26I GTCTC 3 cut(s) 181, 500, 612
AlwI GGATC 1 cut(s) 127
Asp700I GAANNNNTTC 1 cut(s) 417
AsuHPI GGTGA 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 332
BbvCI CCTCAGC 1 cut(s) 18
BccI CCATC 4 cut(s) 110, 202, 215, 344
BciT130I CCWGG 1 cut(s) 612
BciVI GTATCC 1 cut(s) 206
BcoDI GTCTC 3 cut(s) 181, 500, 612
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 3 cut(s) 510, 566, 634
BfuI GTATCC 1 cut(s) 206
Bme1390I CCNGG 1 cut(s) 612
BmgBI CACGTC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 1 cut(s) 612
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 1 cut(s) 430
BmuI ACTGGG 1 cut(s) 313
BplI GAGNNNNNCTC 2 cut(s) 14, 46
Bpu10I CCTNAGC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 445
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 2 cut(s) 308, 535
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 1 cut(s) 445
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 3 cut(s) 9, 45, 266
BseNI ACTGG 2 cut(s) 308, 535
BseRI GAGGAG 2 cut(s) 35, 199
BsiHKAI GWGCWC 1 cut(s) 332
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 3 cut(s) 181, 500, 612
BsmBI CGTCTC 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 332
Bsp143I GATC 2 cut(s) 85, 119
BspCNI CTCAG 3 cut(s) 10, 44, 265
BspLI GGNNCC 1 cut(s) 67
BspPI GGATC 1 cut(s) 127
BspQI GCTCTTC 1 cut(s) 534
BsrI ACTGG 2 cut(s) 308, 535
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 2 cut(s) 85, 119
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 2 cut(s) 218, 557
Bst6I CTCTTC 1 cut(s) 534
BstDEI CTNAG 3 cut(s) 18, 31, 252
BstKTI GATC 2 cut(s) 88, 122
BstMAI GTCTC 3 cut(s) 181, 500, 612
BstMBI GATC 2 cut(s) 85, 119
BstNI CCWGG 1 cut(s) 612
BstSCI CCNGG 1 cut(s) 610
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
BsuI GTATCC 1 cut(s) 206
BtrI CACGTC 1 cut(s) 191
CviAII CATG 3 cut(s) 89, 126, 290
CviJI RGCY 7 cut(s) 25, 46, 158, 233, 273, 338, 586
CviKI_1 RGCY 7 cut(s) 25, 46, 158, 233, 273, 338, 586
DdeI CTNAG 3 cut(s) 18, 31, 252
DpnI GATC 2 cut(s) 87, 121
DpnII GATC 2 cut(s) 85, 119
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco130I CCWWGG 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 445
ErhI CCWWGG 1 cut(s) 445
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 3 cut(s) 92, 129, 293
FaiI YATR 8 cut(s) 90, 127, 167, 204, 230, 291, 356, 411
FatI CATG 3 cut(s) 88, 125, 289
FspBI CTAG 3 cut(s) 510, 566, 634
Hin1II CATG 3 cut(s) 92, 129, 293
HinfI GANTC 4 cut(s) 261, 413, 605, 615
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 41
Hpy188I TCNGA 4 cut(s) 34, 139, 163, 505
Hpy188III TCNNGA 2 cut(s) 254, 347
Hpy8I GTNNAC 1 cut(s) 41
HpyAV CCTTC 2 cut(s) 251, 353
HpyCH4III ACNGT 2 cut(s) 218, 557
HpyCH4IV ACGT 2 cut(s) 78, 190
HpyCH4V TGCA 2 cut(s) 58, 581
HpyF3I CTNAG 3 cut(s) 18, 31, 252
HpySE526I ACGT 2 cut(s) 78, 190
Hsp92II CATG 3 cut(s) 92, 129, 293
Kzo9I GATC 2 cut(s) 85, 119
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 2 cut(s) 22, 591
LpnPI CCDG 8 cut(s) 219, 239, 289, 360, 386, 516, 597, 624
LweI GCATC 1 cut(s) 430
MaeI CTAG 3 cut(s) 510, 566, 634
MaeII ACGT 2 cut(s) 78, 190
MaeIII GTNAC 4 cut(s) 79, 191, 296, 322
MalI GATC 2 cut(s) 87, 121
MboI GATC 2 cut(s) 85, 119
MboII GAAGA 5 cut(s) 114, 125, 240, 392, 551
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 332
MluCI AATT 5 cut(s) 59, 197, 395, 492, 571
MlyI GAGTC 1 cut(s) 614
MnlI CCTC 4 cut(s) 13, 169, 177, 311
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 2 cut(s) 276, 456
MspR9I CCNGG 1 cut(s) 612
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 612
NdeII GATC 2 cut(s) 85, 119
NlaIII CATG 3 cut(s) 92, 129, 293
NlaIV GGNNCC 1 cut(s) 67
NmuCI GTSAC 2 cut(s) 79, 191
PciSI GCTCTTC 1 cut(s) 534
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 3 cut(s) 261, 413, 615
PflMI CCANNNNNTGG 1 cut(s) 208
PfoI TCCNGGA 1 cut(s) 610
PleI GAGTC 1 cut(s) 613
PpsI GAGTC 1 cut(s) 613
Psp6I CCWGG 1 cut(s) 610
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 1 cut(s) 67
PsuI RGATCY 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 2 cut(s) 276, 456
Sau3AI GATC 2 cut(s) 85, 119
SchI GAGTC 1 cut(s) 614
ScrFI CCNGG 1 cut(s) 612
SduI GDGCHC 1 cut(s) 332
SetI ASST 8 cut(s) 27, 48, 81, 99, 193, 235, 322, 588
SfaNI GCATC 1 cut(s) 430
SpeI ACTAGT 1 cut(s) 509
Sse9I AATT 5 cut(s) 59, 197, 395, 492, 571
SspI AATATT 1 cut(s) 268
SspMI CTAG 3 cut(s) 510, 566, 634
StyD4I CCNGG 1 cut(s) 610
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 2 cut(s) 218, 557
TaiI ACGT 2 cut(s) 81, 193
TaqI TCGA 2 cut(s) 74, 107
TasI AATT 5 cut(s) 59, 197, 395, 492, 571
TfiI GAWTC 3 cut(s) 261, 413, 615
Tru1I TTAA 2 cut(s) 276, 456
Tru9I TTAA 2 cut(s) 276, 456
TseFI GTSAC 2 cut(s) 79, 191
Tsp45I GTSAC 2 cut(s) 79, 191
TspDTI ATGAA 6 cut(s) 114, 126, 168, 410, 426, 477
Van91I CCANNNNNTGG 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 3 cut(s) 510, 566, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.