MD00G1188600.v1.1

Plant mobile domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002936
Physical Location & Seq
Forward (+)
44764330 .. 44767174
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1188600.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGTCATGGAGGGAAGTTTTGCTCATGAGCTCTACGCAGAAAGTTTAAGCCTATCAAAAATACAATTGGATGGTGAACCCAGTGCAAACAGTTTAGAAAGTGATCGACAAGACCATGATAGGGTTGAATCGTGTGATGAAGATGGATCTTGGTATGCTTCTGACGATGAATTGCATGAACAATCTGCTTTGGGCCGGGAATGGCAGCGGAGACATAACCAATACCATACGACTGGTTACCGTGATGGGGTAATAGCGGGAAAAGAAGCTTCTTCACAAGAGGGATTTAATATTGGCTTTAAGAAATCGGTCCTTGTTGGGTACAACTGGGGTCTTGTCAGAGGTGTTACTAGTGCGCTGGCTTGCCTTCCAGATGGTTTAAAGGAGAAGTTGATTGAAACAGAAGAACAACGAAATGGATTTCAAAGATTGTTTGAATCAGTGCATTCTATGTCAACGAAAGATGCCCTCGGGTATTTGTACGATGATATGATAGCCAAAAAAGATAGAGAACATAGTGAAATTTCTGATACTACCGGTTCTCTTGAAGTTGGCTCGCCAGAGAAAATAGCACATGTTAGTGGCCTAGGCAATCACTTTGCAGAACTCCAGTCACTTCTTGTCAAGTGTCCTGCAGTTAACGTACATATACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

219

Amino Acids

24.23

Weight (kDa)

4.96

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yae1_N PF09811 79 - 116 2.9e-10 Essential protein Yae1, N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 208, 257
AclWI GGATC 1 cut(s) 154
AcsI RAATTY 1 cut(s) 522
AfaI GTAC 3 cut(s) 323, 482, 645
AfiI CCNNNNNNNGG 2 cut(s) 121, 247
AflIII ACRYGT 1 cut(s) 574
AgeI ACCGGT 1 cut(s) 536
AgsI TTSAA 5 cut(s) 128, 398, 425, 437, 548
AhlI ACTAGT 1 cut(s) 350
AluBI AGCT 2 cut(s) 31, 269
AluI AGCT 2 cut(s) 31, 269
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 2 cut(s) 193, 583
ApeKI GCWGC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 522
AsiGI ACCGGT 1 cut(s) 536
AspA2I CCTAGG 1 cut(s) 586
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 2 cut(s) 193, 310
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 1 cut(s) 310
AvrII CCTAGG 1 cut(s) 586
BanII GRGCYC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 217
BccI CCATC 4 cut(s) 65, 137, 239, 368
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 205
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 2 cut(s) 351, 587
BfmI CTRYAG 1 cut(s) 633
BisI GCNGC 1 cut(s) 206
BlnI CCTAGG 1 cut(s) 586
BlsI GCNGC 1 cut(s) 207
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 193, 310
BmrFI CCNGG 1 cut(s) 197
BmrI ACTGGG 2 cut(s) 75, 337
BmsI GCATC 1 cut(s) 454
BmuI ACTGGG 2 cut(s) 75, 337
BpmI CTGGAG 1 cut(s) 593
BpuMI CCSGG 1 cut(s) 197
BsaJI CCNNGG 2 cut(s) 469, 586
BsaWI WCCGGW 1 cut(s) 536
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 247
Bse118I RCCGGY 1 cut(s) 536
Bse1I ACTGG 4 cut(s) 81, 238, 332, 610
BseDI CCNNGG 2 cut(s) 469, 586
BseGI GGATG 1 cut(s) 76
BseLI CCNNNNNNNGG 2 cut(s) 121, 247
BseNI ACTGG 4 cut(s) 81, 238, 332, 610
BseXI GCAGC 1 cut(s) 217
BshFI GGCC 2 cut(s) 195, 585
BshTI ACCGGT 1 cut(s) 536
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 2 cut(s) 196, 537
BslI CCNNNNNNNGG 2 cut(s) 121, 247
BsmAI GTCTC 1 cut(s) 205
BsmI GAATGC 1 cut(s) 445
BsnI GGCC 2 cut(s) 195, 585
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 2 cut(s) 103, 146
BspACI CCGC 2 cut(s) 208, 257
BspANI GGCC 2 cut(s) 195, 585
BspHI TCATGA 1 cut(s) 25
BspMAI CTGCAG 1 cut(s) 637
BspPI GGATC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 536
BsrI ACTGG 4 cut(s) 81, 238, 332, 610
BssAI RCCGGY 1 cut(s) 536
BssECI CCNNGG 2 cut(s) 469, 586
BssMI GATC 2 cut(s) 103, 146
BssT1I CCWWGG 1 cut(s) 586
Bst4CI ACNGT 3 cut(s) 92, 242, 654
BstC8I GCNNGC 3 cut(s) 360, 364, 557
BstEII GGTNACC 1 cut(s) 236
BstF5I GGATG 1 cut(s) 76
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 2 cut(s) 106, 149
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 103, 146
BstNSI RCATGY 1 cut(s) 578
BstPI GGTNACC 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 633
BstV1I GCAGC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 146
BstXI CCANNNNNNTGG 1 cut(s) 233
BstYI RGATCY 1 cut(s) 146
BsuRI GGCC 2 cut(s) 195, 585
BtsCI GGATG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 88, 447
Cac8I GCNNGC 3 cut(s) 360, 364, 557
CciI TCATGA 1 cut(s) 25
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 1 cut(s) 536
Cfr13I GGNCC 2 cut(s) 193, 310
Csp6I GTAC 3 cut(s) 322, 481, 644
CspAI ACCGGT 1 cut(s) 536
CviAII CATG 5 cut(s) 7, 26, 116, 176, 575
CviJI RGCY 9 cut(s) 31, 51, 195, 269, 297, 362, 497, 555, 585
CviKI_1 RGCY 9 cut(s) 31, 51, 195, 269, 297, 362, 497, 555, 585
CviQI GTAC 3 cut(s) 322, 481, 644
DpnI GATC 2 cut(s) 105, 148
DpnII GATC 2 cut(s) 103, 146
DraI TTTAAA 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 470
Eco91I GGTNACC 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 586
FaeI CATG 5 cut(s) 10, 29, 119, 179, 578
FatI CATG 5 cut(s) 6, 25, 115, 175, 574
FauI CCCGC 1 cut(s) 250
Fnu4HI GCNGC 1 cut(s) 206
FokI GGATG 1 cut(s) 83
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 1 cut(s) 206
FspBI CTAG 2 cut(s) 351, 587
GlaI GCGC 1 cut(s) 357
GluI GCNGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 593
HaeIII GGCC 2 cut(s) 195, 585
HapII CCGG 2 cut(s) 196, 537
HhaI GCGC 1 cut(s) 358
Hin1II CATG 5 cut(s) 10, 29, 119, 179, 578
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HincII GTYRAC 2 cut(s) 456, 640
HindII GTYRAC 2 cut(s) 456, 640
HindIII AAGCTT 1 cut(s) 267
HinfI GANTC 2 cut(s) 128, 437
HpaI GTTAAC 1 cut(s) 640
HpaII CCGG 2 cut(s) 196, 537
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 3 cut(s) 77, 456, 640
Hpy188I TCNGA 3 cut(s) 163, 341, 529
Hpy188III TCNNGA 3 cut(s) 26, 371, 545
Hpy8I GTNNAC 3 cut(s) 77, 456, 640
HpyAV CCTTC 1 cut(s) 377
HpyCH4III ACNGT 3 cut(s) 92, 242, 654
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 5 cut(s) 86, 175, 445, 602, 635
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 5 cut(s) 10, 29, 119, 179, 578
HspAI GCGC 1 cut(s) 356
KspAI GTTAAC 1 cut(s) 640
Kzo9I GATC 2 cut(s) 103, 146
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 1 cut(s) 454
MaeI CTAG 2 cut(s) 351, 587
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 236, 346, 612
MalI GATC 2 cut(s) 105, 148
MboI GATC 2 cut(s) 103, 146
MboII GAAGA 3 cut(s) 152, 264, 416
MfeI CAATTG 1 cut(s) 65
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 3 cut(s) 65, 170, 522
MnlI CCTC 4 cut(s) 4, 274, 335, 479
MseI TTAA 5 cut(s) 47, 288, 300, 380, 639
MslI CAYNNNNRTG 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 2 cut(s) 196, 537
MspR9I CCNGG 1 cut(s) 197
MunI CAATTG 1 cut(s) 65
Mva1269I GAATGC 1 cut(s) 445
NciI CCSGG 1 cut(s) 197
NdeII GATC 2 cut(s) 103, 146
NlaIII CATG 5 cut(s) 10, 29, 119, 179, 578
NmuCI GTSAC 1 cut(s) 612
NspI RCATGY 1 cut(s) 578
PagI TCATGA 1 cut(s) 25
PciI ACATGT 1 cut(s) 574
PctI GAATGC 1 cut(s) 445
PfeI GAWTC 2 cut(s) 128, 437
PinAI ACCGGT 1 cut(s) 536
PkrI GCNGC 1 cut(s) 207
PscI ACATGT 1 cut(s) 574
Psp124BI GAGCTC 1 cut(s) 33
PspEI GGTNACC 1 cut(s) 236
PspPI GGNCC 2 cut(s) 193, 310
PstI CTGCAG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 146
RsaI GTAC 3 cut(s) 323, 482, 645
RsaNI GTAC 3 cut(s) 322, 481, 644
RseI CAYNNNNRTG 1 cut(s) 579
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 5 cut(s) 47, 288, 300, 380, 639
SatI GCNGC 1 cut(s) 206
Sau3AI GATC 2 cut(s) 103, 146
Sau96I GGNCC 2 cut(s) 193, 310
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 1 cut(s) 33
SetI ASST 4 cut(s) 33, 271, 346, 645
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 579
SpeI ACTAGT 1 cut(s) 350
Sse9I AATT 3 cut(s) 65, 170, 522
SsiI CCGC 2 cut(s) 208, 257
SspI AATATT 1 cut(s) 292
SspMI CTAG 2 cut(s) 351, 587
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 3 cut(s) 92, 242, 654
TaiI ACGT 1 cut(s) 645
TaqI TCGA 1 cut(s) 106
TaqII GACCGA 1 cut(s) 298
TasI AATT 3 cut(s) 65, 170, 522
TfiI GAWTC 2 cut(s) 128, 437
Tru1I TTAA 5 cut(s) 47, 288, 300, 380, 639
Tru9I TTAA 5 cut(s) 47, 288, 300, 380, 639
TscAI CASTG 2 cut(s) 88, 447
TseFI GTSAC 1 cut(s) 612
TseI GCWGC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 612
TspDTI ATGAA 3 cut(s) 153, 183, 192
TspRI CASTG 2 cut(s) 88, 447
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 522
XceI RCATGY 1 cut(s) 578
XmaJI CCTAGG 1 cut(s) 586
XspI CTAG 2 cut(s) 351, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.