AT3G19250

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004578
Physical Location & Seq
Reverse (-)
6666609 .. 6668395
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G19250.1

Sequence Viewer

Length: 1083 bp
ATGCCGCACTGTTTTACCTTCAAGCCAGCTTCTCCAGAGGGCTCTCTCGGCGACGACCATCTTCCCCATCCTTCCCCAGAGGGAAGTGTTGCCTCCACTTTTAACCTCAGCCACGAGTTAGCTCATGCTTTTCAAACCCCGTCGTACCACGACATACGTTCACGGCTTCTTGTTATTGACCCAACACAAGAAAATCTCGAGCTGTTTCTTTCTCAAGAGCTCAGACCAAACAACGAATCCGTTCAAGAAGCTCTTAGTCTTAGACACGCCAAGCAAACTACACTTACCAATCTTGTCTCTACTTACTTCCAACACAGCGAAGACGCCACCCGATTTTGCTTGAATCTTTACCAAAACGTCCACAGTGCTCGCTGCCACCTCTACACACCCCTCTTAGACCTCTTCAATATCTTTCCCCGTGATTCCCATTCCGCAATTGACGAGTCCTTCTGTAACTTAGCATTCGACGTTTTTCTTAAGCTTGACACGTTTGAAAACCCTTTCGCATCTCCTGAATCTCACAGCTTTCAAGACACCCAATTATGCTTTTATCAGCTGGCTGACAAGCTGGACACCCGTATTCGTAAGTCTAAGTCACGGGTCCGCCTCCTCCATCATGCCACAGCTGGTTCTGCCTTATGTCTTGTAACTGCTGTTGTTGTGGTTGCTGCTTCTGCGGCATTTATCGCATATCATGCACTTCCCACCATTCTTGTTGTTGCAGGTCCCTTGTGCACTCCCTACCTCCCTCACAGTTTCAAGAAGAAGGAGTTGTCTAACATTTTTCAGCTAAATGTTGCTGCCAAGGGTACTTTTGCGCTCAACAAAGATCTTGACACCATTGACCGTCTTGTCTCCCGTTTGCACACGGGGGTTAAAAACGACAAGCTTCTTATTCGCCTTGGATTAGAGAGGGGGAGGGACGTGTATACAATTCCGGAGTTTGTGAAACAGCTTCGAAAGAGCCATGTTAACCACACCCATCAACTCGAGGTTCTTGTGGATCATATATGTCGTTGGTTCACTAATGTTAACAAATCCAGATCGTTGCTCCTTAAAGAGATCCTTCGTCCCCAAACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

360

Amino Acids

40.77

Weight (kDa)

7.73

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 91 - 355 5.8e-09 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 851
Acc36I ACCTGC 1 cut(s) 713
AccI GTMKAC 1 cut(s) 929
AccIII TCCGGA 1 cut(s) 937
AciI CCGC 4 cut(s) 5, 432, 604, 677
AclWI GGATC 2 cut(s) 1011, 1057
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 2 cut(s) 146, 811
AflII CTTAAG 1 cut(s) 476
AflIII ACRYGT 2 cut(s) 486, 924
AgsI TTSAA 8 cut(s) 22, 134, 245, 343, 406, 494, 530, 760
AjiI CACGTC 1 cut(s) 925
Alw21I GWGCWC 3 cut(s) 222, 370, 737
Alw26I GTCTC 2 cut(s) 301, 859
Alw44I GTGCAC 1 cut(s) 733
AlwI GGATC 2 cut(s) 1011, 1057
Ama87I CYCGRG 2 cut(s) 197, 989
Aor13HI TCCGGA 1 cut(s) 937
ApaLI GTGCAC 1 cut(s) 733
ApeKI GCWGC 3 cut(s) 372, 668, 800
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 2 cut(s) 601, 725
AsuII TTCGAA 1 cut(s) 958
AvaI CYCGRG 2 cut(s) 197, 989
AvaII GGWCC 2 cut(s) 601, 725
BaeGI GKGCMC 1 cut(s) 737
BanII GRGCYC 2 cut(s) 44, 222
BauI CACGAG 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 3 cut(s) 222, 370, 737
BbvCI CCTCAGC 1 cut(s) 107
BbvI GCAGC 3 cut(s) 359, 655, 787
BccI CCATC 4 cut(s) 66, 75, 621, 990
BceAI ACGGC 1 cut(s) 179
BcoDI GTCTC 2 cut(s) 301, 859
BfrI CTTAAG 1 cut(s) 476
BfuAI ACCTGC 1 cut(s) 713
BglII AGATCT 1 cut(s) 829
BisI GCNGC 5 cut(s) 5, 373, 669, 678, 801
BlsI GCNGC 5 cut(s) 6, 374, 670, 679, 802
Bme18I GGWCC 2 cut(s) 601, 725
BmeT110I CYCGRG 2 cut(s) 197, 989
BmgBI CACGTC 1 cut(s) 925
BmgT120I GGNCC 2 cut(s) 601, 725
BmiI GGNNCC 2 cut(s) 602, 727
BmsI GCATC 1 cut(s) 515
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 107
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 804, 901
BsaWI WCCGGW 1 cut(s) 937
BseAI TCCGGA 1 cut(s) 937
BseDI CCNNGG 2 cut(s) 804, 901
BseGI GGATG 1 cut(s) 67
BseMII CTCAG 2 cut(s) 121, 235
BseRI GAGGAG 1 cut(s) 599
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 3 cut(s) 359, 655, 787
BsiHKAI GWGCWC 3 cut(s) 222, 370, 737
BsiHKCI CYCGRG 2 cut(s) 197, 989
BsiSI CCGG 1 cut(s) 938
BslFI GGGAC 3 cut(s) 711, 935, 1056
BsmAI GTCTC 2 cut(s) 301, 859
BsmFI GGGAC 3 cut(s) 711, 935, 1056
BsmI GAATGC 1 cut(s) 461
BsoBI CYCGRG 2 cut(s) 197, 989
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 4 cut(s) 44, 222, 370, 737
Bsp13I TCCGGA 1 cut(s) 937
Bsp143I GATC 4 cut(s) 829, 1003, 1043, 1062
BspACI CCGC 4 cut(s) 5, 432, 604, 677
BspCNI CTCAG 2 cut(s) 120, 234
BspEI TCCGGA 1 cut(s) 937
BspLI GGNNCC 2 cut(s) 602, 727
BspMI ACCTGC 1 cut(s) 713
BspPI GGATC 2 cut(s) 1011, 1057
BspT104I TTCGAA 1 cut(s) 958
BspTI CTTAAG 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 804, 901
BssMI GATC 4 cut(s) 829, 1003, 1043, 1062
BssNAI GTATAC 1 cut(s) 930
BssNI GRCGYC 1 cut(s) 324
BssSI CACGAG 1 cut(s) 113
BssT1I CCWWGG 2 cut(s) 804, 901
Bst1107I GTATAC 1 cut(s) 930
Bst2BI CACGAG 1 cut(s) 113
Bst4CI ACNGT 4 cut(s) 11, 365, 755, 848
Bst6I CTCTTC 1 cut(s) 407
BstACI GRCGYC 1 cut(s) 324
BstAFI CTTAAG 1 cut(s) 476
BstAPI GCANNNNNTGC 1 cut(s) 695
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 3 cut(s) 27, 370, 558
BstDEI CTNAG 7 cut(s) 107, 221, 254, 260, 394, 457, 591
BstF5I GGATG 1 cut(s) 67
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 4 cut(s) 832, 1006, 1046, 1065
BstMAI GTCTC 2 cut(s) 301, 859
BstMBI GATC 4 cut(s) 829, 1003, 1043, 1062
BstMWI GCNNNNNNNGC 6 cut(s) 48, 632, 674, 677, 686, 695
BstSLI GKGCMC 1 cut(s) 737
BstV1I GCAGC 3 cut(s) 359, 655, 787
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 2 cut(s) 829, 1062
BstYI RGATCY 2 cut(s) 829, 1062
BstZ17I GTATAC 1 cut(s) 930
BtrI CACGTC 1 cut(s) 925
BtsCI GGATG 1 cut(s) 67
BtsIMutI CAGTG 2 cut(s) 7, 370
BveI ACCTGC 1 cut(s) 713
Cac8I GCNNGC 3 cut(s) 27, 370, 558
CfoI GCGC 1 cut(s) 820
Cfr13I GGNCC 2 cut(s) 601, 725
CseI GACGC 1 cut(s) 332
Csp6I GTAC 2 cut(s) 145, 810
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 4 cut(s) 125, 617, 695, 968
CviQI GTAC 2 cut(s) 145, 810
DdeI CTNAG 7 cut(s) 107, 221, 254, 260, 394, 457, 591
DpnI GATC 4 cut(s) 831, 1005, 1045, 1064
DpnII GATC 4 cut(s) 829, 1003, 1043, 1062
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
EciI GGCGGA 1 cut(s) 593
Ecl136II GAGCTC 1 cut(s) 220
Eco130I CCWWGG 2 cut(s) 804, 901
Eco24I GRGCYC 2 cut(s) 44, 222
Eco47I GGWCC 2 cut(s) 601, 725
Eco53kI GAGCTC 1 cut(s) 220
Eco88I CYCGRG 2 cut(s) 197, 989
EcoICRI GAGCTC 1 cut(s) 220
EcoO109I RGGNCCY 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 804, 901
EcoT38I GRGCYC 2 cut(s) 44, 222
ErhI CCWWGG 2 cut(s) 804, 901
FaeI CATG 4 cut(s) 128, 620, 698, 971
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 1050, 1082
FaqI GGGAC 3 cut(s) 711, 935, 1056
FatI CATG 4 cut(s) 124, 616, 694, 967
FblI GTMKAC 1 cut(s) 929
Fnu4HI GCNGC 5 cut(s) 5, 373, 669, 678, 801
FokI GGATG 1 cut(s) 54
FriOI GRGCYC 2 cut(s) 44, 222
Fsp4HI GCNGC 5 cut(s) 5, 373, 669, 678, 801
GlaI GCGC 1 cut(s) 819
GluI GCNGC 5 cut(s) 5, 373, 669, 678, 801
GsuI CTGGAG 1 cut(s) 18
HapII CCGG 1 cut(s) 938
HgaI GACGC 1 cut(s) 332
HhaI GCGC 1 cut(s) 820
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 4 cut(s) 128, 620, 698, 971
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HincII GTYRAC 2 cut(s) 973, 1033
HindII GTYRAC 2 cut(s) 973, 1033
HindIII AAGCTT 2 cut(s) 479, 887
HinfI GANTC 5 cut(s) 236, 343, 422, 443, 515
HpaI GTTAAC 2 cut(s) 973, 1033
HpaII CCGG 1 cut(s) 938
Hpy166II GTNNAC 7 cut(s) 161, 361, 735, 930, 973, 1023, 1033
Hpy188I TCNGA 1 cut(s) 224
Hpy8I GTNNAC 7 cut(s) 161, 361, 735, 930, 973, 1023, 1033
Hpy99I CGWCG 3 cut(s) 56, 145, 470
HpyAV CCTTC 5 cut(s) 28, 81, 457, 760, 1076
HpyCH4III ACNGT 4 cut(s) 11, 365, 755, 848
HpyCH4IV ACGT 6 cut(s) 157, 357, 468, 488, 924, 1079
HpyCH4V TGCA 4 cut(s) 698, 722, 735, 865
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 632, 674, 677, 686, 695
HpyF3I CTNAG 7 cut(s) 107, 221, 254, 260, 394, 457, 591
HpySE526I ACGT 6 cut(s) 157, 357, 468, 488, 924, 1079
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 4 cut(s) 128, 620, 698, 971
HspAI GCGC 1 cut(s) 818
Kpn2I TCCGGA 1 cut(s) 937
KspAI GTTAAC 2 cut(s) 973, 1033
Kzo9I GATC 4 cut(s) 829, 1003, 1043, 1062
LmnI GCTCC 1 cut(s) 1056
Lsp1109I GCAGC 3 cut(s) 359, 655, 787
LweI GCATC 1 cut(s) 515
MaeII ACGT 6 cut(s) 157, 357, 468, 488, 924, 1079
MaeIII GTNAC 3 cut(s) 452, 594, 646
MalI GATC 4 cut(s) 831, 1005, 1045, 1064
MboI GATC 4 cut(s) 829, 1003, 1043, 1062
MboII GAAGA 4 cut(s) 53, 332, 394, 775
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 2 cut(s) 829, 1062
MhlI GDGCHC 4 cut(s) 44, 222, 370, 737
MluCI AATT 3 cut(s) 435, 539, 933
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 334
MroI TCCGGA 1 cut(s) 937
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 6 cut(s) 102, 477, 876, 972, 1032, 1056
MspA1I CMGCKG 2 cut(s) 556, 626
MspCI CTTAAG 1 cut(s) 476
MspI CCGG 1 cut(s) 938
MunI CAATTG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 461
MwoI GCNNNNNNNGC 6 cut(s) 48, 632, 674, 677, 686, 695
NdeII GATC 4 cut(s) 829, 1003, 1043, 1062
NlaIII CATG 4 cut(s) 128, 620, 698, 971
NlaIV GGNNCC 2 cut(s) 602, 727
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 1 cut(s) 594
NspV TTCGAA 1 cut(s) 958
PaeR7I CTCGAG 2 cut(s) 197, 989
PctI GAATGC 1 cut(s) 461
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 4 cut(s) 236, 343, 422, 515
PkrI GCNGC 5 cut(s) 6, 374, 670, 679, 802
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PpuMI RGGWCCY 1 cut(s) 725
Psp124BI GAGCTC 1 cut(s) 222
Psp5II RGGWCCY 1 cut(s) 725
PspN4I GGNNCC 2 cut(s) 602, 727
PspPI GGNCC 2 cut(s) 601, 725
PspPPI RGGWCCY 1 cut(s) 725
PspXI VCTCGAGB 1 cut(s) 989
PsuI RGATCY 2 cut(s) 829, 1062
PvuII CAGCTG 2 cut(s) 556, 626
RsaI GTAC 2 cut(s) 146, 811
RsaNI GTAC 2 cut(s) 145, 810
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 6 cut(s) 102, 477, 876, 972, 1032, 1056
SatI GCNGC 5 cut(s) 5, 373, 669, 678, 801
Sau3AI GATC 4 cut(s) 829, 1003, 1043, 1062
Sau96I GGNCC 2 cut(s) 601, 725
SchI GAGTC 1 cut(s) 452
SduI GDGCHC 4 cut(s) 44, 222, 370, 737
SfaNI GCATC 1 cut(s) 515
Sfr274I CTCGAG 2 cut(s) 197, 989
SfuI TTCGAA 1 cut(s) 958
SinI GGWCC 2 cut(s) 601, 725
SlaI CTCGAG 2 cut(s) 197, 989
SmlI CTYRAG 4 cut(s) 197, 213, 476, 989
SmoI CTYRAG 4 cut(s) 197, 213, 476, 989
Sse9I AATT 3 cut(s) 435, 539, 933
SsiI CCGC 4 cut(s) 5, 432, 604, 677
SstI GAGCTC 1 cut(s) 222
StyI CCWWGG 2 cut(s) 804, 901
TaaI ACNGT 4 cut(s) 11, 365, 755, 848
TaiI ACGT 6 cut(s) 160, 360, 471, 491, 927, 1082
TaqI TCGA 4 cut(s) 198, 465, 958, 990
TasI AATT 3 cut(s) 435, 539, 933
TauI GCSGC 2 cut(s) 7, 680
TfiI GAWTC 4 cut(s) 236, 343, 422, 515
Tru1I TTAA 6 cut(s) 102, 477, 876, 972, 1032, 1056
Tru9I TTAA 6 cut(s) 102, 477, 876, 972, 1032, 1056
TscAI CASTG 2 cut(s) 14, 370
TseFI GTSAC 1 cut(s) 594
TseI GCWGC 3 cut(s) 372, 668, 800
Tsp45I GTSAC 1 cut(s) 594
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 2 cut(s) 14, 370
Vha464I CTTAAG 1 cut(s) 476
VneI GTGCAC 1 cut(s) 733
VpaK11BI GGWCC 2 cut(s) 601, 725
XhoI CTCGAG 2 cut(s) 197, 989
XmiI GTMKAC 1 cut(s) 929
XmnI GAANNNNTTC 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.