MD09G1002000.v1.1

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004578
Physical Location & Seq
Forward (+)
246106 .. 248676
2571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1002000.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGCTGCAATGTCTCTCTCTGAAATTATCGTCGTCGTCGTCACCCTCCTCAGCAGCAGCAGCGGCTCCCAATGGCAATACTCATTCTCATCCGCCTCCCCCGTCGCAAGGAGGAACGAGCTCTGAGGCATCGCCCTCCGCCACTCTCACCCACGAGTACACCCGCGCCGTCCAAAGCCAATCCTACAATGAAATTTGGTCCAAGGTTCATCAGGTCCACGACCACCACCATGATCCTGGTCATGATGAGCTGCCGCTGGAGGAGGAGGACCACGACGAATCGGATAATGAAGAGAGCCGCCACCGCCGGAGTCAGAGGCAGCACCAGCTTCTGTTGGGGCGAGTGCTTCAGCCCAACCGAGAGTGCGTCCAAGAGGCGCTTCGCCTCGCCAAGCCCAACTCCCTCACTCGCCTGGTCTCCGACTACTTCGACCAGAGCGAGAACACGACCCAAGCCTGCCTGCTGCTCCTCCACCGCAGCGTCTATCGAGCACGCGAGCTCTATGCTCCGCTCCACCAACTCCTCGACGCCGTCGTCCACTCTGACTCTGTGGAGTCGTCACTGAGCCTCAACCTCAGCCTCAGCCAGTCCCAGTGCCGTCGGGCCTTGGACGTCTTTCTTCAGTTCGACCTCCACGACAACCCCTTCCCTTCCCCTGACGACGCCGACTCCCCCAATTTGGACAACATGCGCCGTTGCTTCTCCCAGCTCAAGCTCCAGCTCGACAGCCGCCTCCGCTCCTCCCGCTCCAAAATCCGCCTCATTCGCCGCGCCACTCTTGCCTCCGCCCTCTGCTTCATTGGCACTGCCGTTGGAGTCGCCGTCTCTGCTGTGGCTGTCACAGTCCACGCTCTCACTGCCGCCCTCGTCGTTGTTGCAGCGCCGCCTTTGTGCTGCACTGCCACTAGCACTGGCTCTGGCTCTGGTAGAAGCACCAGTTGTTGCAATTGCCGCACCATGACTACCACTGAGAAGAAGGAAATTGCCAATATGAAGCAACTTGATGCTGCTGCCATGGGTACTTGTGTCCTCGACAATGACCTTGCCACCATTGACCGACTCGTTCGCCGCCTCCATGCCGCAGTGGAGGGGGACAAGCTTCTGGTTCGCCTGGGATTGGAGAGGGGATCTGTCAGTGGCACTGCGGTCGACAAGCATCCAATTCAGGAGGTGCTGAAACAGCTGCACAAGAGCCACCCCAATTTCCTGCAACTTGTGGACGAACTTGAGGAGCATATATGCCTCTGCTTTAACACCGTCAACAGGTCCAGATCGCTGCTTTTCCAGCACATCTATACACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

435

Amino Acids

47.84

Weight (kDa)

6.53

Isoelectric Point (pI)

61.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 130 - 431 6.3e-10 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
AatII GACGTC 1 cut(s) 615
AccBSI CCGCTC 3 cut(s) 511, 738, 747
AccI GTMKAC 1 cut(s) 1149
AccII CGCG 3 cut(s) 165, 495, 771
AclWI GGATC 2 cut(s) 227, 1135
AcsI RAATTY 1 cut(s) 192
AcuI CTGAAG 2 cut(s) 332, 605
AcyI GRCGYC 3 cut(s) 528, 612, 663
AfaI GTAC 2 cut(s) 158, 1021
AfiI CCNNNNNNNGG 4 cut(s) 107, 679, 1117, 1263
AjnI CCWGG 3 cut(s) 235, 411, 1110
AjuI GAANNNNNNNTTGG 2 cut(s) 363, 395
AluBI AGCT 9 cut(s) 120, 250, 328, 499, 709, 715, 721, 1099, 1183
AluI AGCT 9 cut(s) 120, 250, 328, 499, 709, 715, 721, 1099, 1183
Alw21I GWGCWC 3 cut(s) 122, 493, 501
Alw26I GTCTC 3 cut(s) 17, 421, 829
AlwI GGATC 2 cut(s) 227, 1135
AlwNI CAGNNNCTG 1 cut(s) 331
AoxI GGCC 1 cut(s) 603
ApoI RAATTY 1 cut(s) 192
AspLEI GCGC 5 cut(s) 167, 379, 693, 773, 883
AspS9I GGNCC 5 cut(s) 198, 214, 268, 603, 1266
AsuHPI GGTGA 2 cut(s) 33, 139
AvaII GGWCC 4 cut(s) 198, 214, 268, 1266
BaeI ACNNNNGTAYC 2 cut(s) 1011, 1044
BanII GRGCYC 2 cut(s) 122, 501
BauI CACGAG 1 cut(s) 152
Bbv12I GWGCWC 3 cut(s) 122, 493, 501
BbvCI CCTCAGC 3 cut(s) 49, 575, 581
BceAI ACGGC 6 cut(s) 152, 515, 582, 678, 794, 806
BcgI CGANNNNNNTGC 4 cut(s) 485, 519, 1129, 1163
BciT130I CCWGG 3 cut(s) 237, 413, 1112
BcoDI GTCTC 3 cut(s) 17, 421, 829
BfaI CTAG 1 cut(s) 906
BfoI RGCGCY 2 cut(s) 380, 884
Bme1390I CCNGG 3 cut(s) 237, 413, 1112
Bme18I GGWCC 4 cut(s) 198, 214, 268, 1266
BmgT120I GGNCC 5 cut(s) 198, 214, 268, 603, 1266
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 3 cut(s) 237, 413, 1112
BmrI ACTGGG 1 cut(s) 586
BmsI GCATC 3 cut(s) 137, 994, 1165
BmuI ACTGGG 1 cut(s) 586
BpmI CTGGAG 2 cut(s) 278, 701
Bpu10I CCTNAGC 3 cut(s) 49, 575, 581
BpuEI CTTGAG 2 cut(s) 695, 1247
BsaHI GRCGYC 3 cut(s) 528, 612, 663
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 4 cut(s) 201, 606, 1014, 1111
BsaXI ACNNNNNCTCC 4 cut(s) 653, 683, 807, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 679, 1117, 1263
Bse1I ACTGG 4 cut(s) 586, 592, 916, 936
Bse3DI GCAATG 1 cut(s) 14
BseBI CCWGG 3 cut(s) 237, 413, 1112
BseDI CCNNGG 4 cut(s) 201, 606, 1014, 1111
BseGI GGATG 2 cut(s) 88, 1156
BseLI CCNNNNNNNGG 4 cut(s) 107, 679, 1117, 1263
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 6 cut(s) 63, 114, 554, 589, 595, 960
BseNI ACTGG 4 cut(s) 586, 592, 916, 936
BseRI GAGGAG 7 cut(s) 37, 275, 278, 458, 512, 730, 1244
BseYI CCCAGC 1 cut(s) 705
BsgI GTGCAG 2 cut(s) 880, 1169
Bsh1236I CGCG 3 cut(s) 165, 495, 771
Bsh1285I CGRYCG 1 cut(s) 1149
BshFI GGCC 1 cut(s) 605
BsiEI CGRYCG 1 cut(s) 1149
BsiHKAI GWGCWC 3 cut(s) 122, 493, 501
BsiSI CCGG 1 cut(s) 307
BslFI GGGAC 2 cut(s) 574, 1106
BslI CCNNNNNNNGG 4 cut(s) 107, 679, 1117, 1263
BsmAI GTCTC 3 cut(s) 17, 421, 829
BsmBI CGTCTC 1 cut(s) 829
BsmFI GGGAC 2 cut(s) 574, 1106
BsnI GGCC 1 cut(s) 605
Bso31I GGTCTC 1 cut(s) 421
Bsp1286I GDGCHC 3 cut(s) 122, 493, 501
Bsp143I GATC 3 cut(s) 232, 1127, 1271
Bsp19I CCATGG 1 cut(s) 1014
BspANI GGCC 1 cut(s) 605
BspCNI CTCAG 6 cut(s) 62, 115, 555, 588, 594, 961
BspFNI CGCG 3 cut(s) 165, 495, 771
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 66
BspPI GGATC 2 cut(s) 227, 1135
BspTNI GGTCTC 1 cut(s) 421
BsrBI CCGCTC 3 cut(s) 511, 738, 747
BsrDI GCAATG 1 cut(s) 14
BsrI ACTGG 4 cut(s) 586, 592, 916, 936
BssECI CCNNGG 4 cut(s) 201, 606, 1014, 1111
BssMI GATC 3 cut(s) 232, 1127, 1271
BssNI GRCGYC 3 cut(s) 528, 612, 663
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 3 cut(s) 201, 606, 1014
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 3 cut(s) 237, 413, 1112
Bst4CI ACNGT 2 cut(s) 844, 1258
Bst6I CTCTTC 1 cut(s) 285
BstACI GRCGYC 3 cut(s) 528, 612, 663
BstC8I GCNNGC 4 cut(s) 457, 461, 493, 497
BstDEI CTNAG 6 cut(s) 49, 123, 563, 575, 581, 969
BstDSI CCRYGG 1 cut(s) 1014
BstF5I GGATG 2 cut(s) 88, 1156
BstFNI CGCG 3 cut(s) 165, 495, 771
BstH2I RGCGCY 2 cut(s) 380, 884
BstHHI GCGC 5 cut(s) 167, 379, 693, 773, 883
BstKTI GATC 3 cut(s) 235, 1130, 1274
BstMAI GTCTC 3 cut(s) 17, 421, 829
BstMBI GATC 3 cut(s) 232, 1127, 1271
BstMCI CGRYCG 1 cut(s) 1149
BstNI CCWGG 3 cut(s) 237, 413, 1112
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 3 cut(s) 235, 411, 1110
BstUI CGCG 3 cut(s) 165, 495, 771
BstX2I RGATCY 1 cut(s) 1127
BstXI CCANNNNNNTGG 1 cut(s) 236
BstYI RGATCY 1 cut(s) 1127
BsuRI GGCC 1 cut(s) 605
BtgI CCRYGG 1 cut(s) 1014
BtgZI GCGATG 1 cut(s) 114
BtsCI GGATG 2 cut(s) 88, 1156
BtsI GCAGTG 5 cut(s) 804, 855, 897, 1089, 1140
Cac8I GCNNGC 4 cut(s) 457, 461, 493, 497
CaiI CAGNNNCTG 1 cut(s) 331
CciI TCATGA 1 cut(s) 241
CfoI GCGC 5 cut(s) 167, 379, 693, 773, 883
Cfr13I GGNCC 5 cut(s) 198, 214, 268, 603, 1266
CseI GACGC 4 cut(s) 355, 469, 536, 671
Csp6I GTAC 2 cut(s) 157, 1020
CviAII CATG 6 cut(s) 230, 242, 688, 958, 1015, 1076
CviQI GTAC 2 cut(s) 157, 1020
DdeI CTNAG 6 cut(s) 49, 123, 563, 575, 581, 969
DpnI GATC 3 cut(s) 234, 1129, 1273
DpnII GATC 3 cut(s) 232, 1127, 1271
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
EciI GGCGGA 4 cut(s) 81, 127, 746, 775
Ecl136II GAGCTC 2 cut(s) 120, 499
Eco130I CCWWGG 3 cut(s) 201, 606, 1014
Eco24I GRGCYC 2 cut(s) 122, 501
Eco31I GGTCTC 1 cut(s) 421
Eco47I GGWCC 4 cut(s) 198, 214, 268, 1266
Eco53kI GAGCTC 2 cut(s) 120, 499
Eco57I CTGAAG 2 cut(s) 332, 605
EcoICRI GAGCTC 2 cut(s) 120, 499
EcoRII CCWGG 3 cut(s) 235, 411, 1110
EcoT14I CCWWGG 3 cut(s) 201, 606, 1014
EcoT38I GRGCYC 2 cut(s) 122, 501
ErhI CCWWGG 3 cut(s) 201, 606, 1014
Esp3I CGTCTC 1 cut(s) 829
FaeI CATG 6 cut(s) 233, 245, 691, 961, 1018, 1079
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 2 cut(s) 574, 1106
FatI CATG 6 cut(s) 229, 241, 687, 957, 1014, 1075
FauI CCCGC 2 cut(s) 170, 752
FblI GTMKAC 1 cut(s) 1149
FokI GGATG 2 cut(s) 75, 1143
FriOI GRGCYC 2 cut(s) 122, 501
FspBI CTAG 1 cut(s) 906
GlaI GCGC 5 cut(s) 166, 378, 692, 772, 882
GsaI CCCAGC 1 cut(s) 709
GsuI CTGGAG 2 cut(s) 278, 701
HaeII RGCGCY 2 cut(s) 380, 884
HaeIII GGCC 1 cut(s) 605
HapII CCGG 1 cut(s) 307
HgaI GACGC 4 cut(s) 355, 469, 536, 671
HhaI GCGC 5 cut(s) 167, 379, 693, 773, 883
Hin1I GRCGYC 3 cut(s) 528, 612, 663
Hin1II CATG 6 cut(s) 233, 245, 691, 961, 1018, 1079
Hin6I GCGC 5 cut(s) 165, 377, 691, 771, 881
HinP1I GCGC 5 cut(s) 165, 377, 691, 771, 881
HincII GTYRAC 2 cut(s) 1150, 1261
HindII GTYRAC 2 cut(s) 1150, 1261
HindIII AAGCTT 1 cut(s) 1097
HinfI GANTC 7 cut(s) 278, 310, 545, 554, 668, 816, 1059
HpaII CCGG 1 cut(s) 307
HphI GGTGA 2 cut(s) 33, 139
Hpy166II GTNNAC 7 cut(s) 159, 217, 538, 847, 1150, 1219, 1261
Hpy188I TCNGA 6 cut(s) 21, 124, 283, 315, 421, 544
Hpy188III TCNNGA 3 cut(s) 242, 1166, 1269
Hpy8I GTNNAC 7 cut(s) 159, 217, 538, 847, 1150, 1219, 1261
HpyAV CCTTC 3 cut(s) 655, 660, 970
HpyCH4III ACNGT 2 cut(s) 844, 1258
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 6 cut(s) 7, 878, 897, 945, 1186, 1210
HpyF3I CTNAG 6 cut(s) 49, 123, 563, 575, 581, 969
HpySE526I ACGT 1 cut(s) 612
Hsp92I GRCGYC 3 cut(s) 528, 612, 663
Hsp92II CATG 6 cut(s) 233, 245, 691, 961, 1018, 1079
HspAI GCGC 5 cut(s) 165, 377, 691, 771, 881
Kzo9I GATC 3 cut(s) 232, 1127, 1271
LmnI GCTCC 8 cut(s) 70, 471, 511, 516, 720, 743, 752, 1231
LweI GCATC 3 cut(s) 137, 994, 1165
MaeI CTAG 1 cut(s) 906
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 3 cut(s) 39, 558, 838
MalI GATC 3 cut(s) 234, 1129, 1273
MbiI CCGCTC 3 cut(s) 511, 738, 747
MboI GATC 3 cut(s) 232, 1127, 1271
MboII GAAGA 3 cut(s) 302, 611, 985
MfeI CAATTG 1 cut(s) 946
MflI RGATCY 1 cut(s) 1127
MhlI GDGCHC 3 cut(s) 122, 493, 501
MluCI AATT 7 cut(s) 23, 192, 676, 946, 981, 1161, 1201
MlyI GAGTC 6 cut(s) 319, 539, 563, 662, 825, 1053
MmeI TCCRAC 2 cut(s) 444, 793
MseI TTAA 2 cut(s) 1251, 1303
MslI CAYNNNNRTG 1 cut(s) 228
MspA1I CMGCKG 3 cut(s) 62, 256, 1183
MspI CCGG 1 cut(s) 307
MspR9I CCNGG 3 cut(s) 237, 413, 1112
MunI CAATTG 1 cut(s) 946
MvaI CCWGG 3 cut(s) 237, 413, 1112
MvnI CGCG 3 cut(s) 165, 495, 771
NcoI CCATGG 1 cut(s) 1014
NdeII GATC 3 cut(s) 232, 1127, 1271
NlaIII CATG 6 cut(s) 233, 245, 691, 961, 1018, 1079
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 3 cut(s) 39, 558, 838
NspI RCATGY 1 cut(s) 691
PagI TCATGA 1 cut(s) 241
PcsI WCGNNNNNNNCGW 4 cut(s) 35, 435, 531, 633
PfeI GAWTC 1 cut(s) 278
PflFI GACNNNGTC 1 cut(s) 530
PleI GAGTC 6 cut(s) 318, 539, 562, 662, 824, 1053
PpsI GAGTC 6 cut(s) 318, 539, 562, 662, 824, 1053
Psp124BI GAGCTC 2 cut(s) 122, 501
Psp6I CCWGG 3 cut(s) 235, 411, 1110
PspFI CCCAGC 1 cut(s) 705
PspGI CCWGG 3 cut(s) 235, 411, 1110
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 5 cut(s) 198, 214, 268, 603, 1266
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 1127
PsyI GACNNNGTC 1 cut(s) 530
PvuII CAGCTG 1 cut(s) 1183
RsaI GTAC 2 cut(s) 158, 1021
RsaNI GTAC 2 cut(s) 157, 1020
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 2 cut(s) 122, 501
SalI GTCGAC 1 cut(s) 1148
SaqAI TTAA 2 cut(s) 1251, 1303
Sau3AI GATC 3 cut(s) 232, 1127, 1271
Sau96I GGNCC 5 cut(s) 198, 214, 268, 603, 1266
SchI GAGTC 6 cut(s) 319, 539, 563, 662, 825, 1053
ScrFI CCNGG 3 cut(s) 237, 413, 1112
SduI GDGCHC 3 cut(s) 122, 493, 501
SfaNI GCATC 3 cut(s) 137, 994, 1165
SinI GGWCC 4 cut(s) 198, 214, 268, 1266
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 2 cut(s) 710, 1226
SmoI CTYRAG 2 cut(s) 710, 1226
Sse9I AATT 7 cut(s) 23, 192, 676, 946, 981, 1161, 1201
SspMI CTAG 1 cut(s) 906
SstI GAGCTC 2 cut(s) 122, 501
StyD4I CCNGG 3 cut(s) 235, 411, 1110
StyI CCWWGG 3 cut(s) 201, 606, 1014
TaaI ACNGT 2 cut(s) 844, 1258
TaiI ACGT 1 cut(s) 615
TaqI TCGA 7 cut(s) 429, 487, 525, 627, 723, 1032, 1149
TaqII GACCGA 1 cut(s) 1071
TasI AATT 7 cut(s) 23, 192, 676, 946, 981, 1161, 1201
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 2 cut(s) 1251, 1303
Tru9I TTAA 2 cut(s) 1251, 1303
TseFI GTSAC 3 cut(s) 39, 558, 838
Tsp45I GTSAC 3 cut(s) 39, 558, 838
TspDTI ATGAA 5 cut(s) 197, 204, 303, 787, 1007
Tth111I GACNNNGTC 1 cut(s) 530
VpaK11BI GGWCC 4 cut(s) 198, 214, 268, 1266
XapI RAATTY 1 cut(s) 192
XceI RCATGY 1 cut(s) 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 1149
XspI CTAG 1 cut(s) 906
ZraI GACGTC 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.