MD09G1001900.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004578
Physical Location & Seq
Forward (+)
242510 .. 245963
3454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1001900.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGTCAGCGTCTCTTGCAGCAATACTAGGAGGTGCTGCAGGAGCCGTGACTTTGGTGGGGATAAGCATCATACTCATATGGTTCTGTGTATCTCGCAATAGGAGTGTTCCAAGAACTTCTGAAACGGGTTCTTCAGATCCTTCTATTCAAGTGGGAAGACATGGAGGGGTTGAGTTGTCAATGCGAGAAACAAGGTGTTTTCATATGGAAGAACTGTCTCTGGCCACAAAACATTTCAGTGATAAAAATTTGATTGGGCAAGGAAAATTTGGGGAGGTGTATATGGGATTTCTCCAGGATGGAATGCTTGTGGCCATCAAAAAGCGACCTGGAACAGCTAGTCAGGAATTTATCAATGAGGTACACTATCTCTCAGCTATTCAGCATCGGAATATTGTTACTCTCTTGGGTTACTGCCAAGAAAATAATCTACAGATTCTTGTGTACGAGTACATTCATAGTGGAAGTGTTTCCAGTCACTTGTACGGCACTGGCCAGCATTCACGTGAAAAGCTAGAATTCAAGAACCGCCTATCAATAGCTCAAGGGGCCGCTAAAGGACTTTCAGATGTTCTAAAGTTCCTGCTAAAGTTCCTGCTTCTTTTTTGTGAGAAGTCTGGAAGCAACGGTCTCCTATTTATTAGAACTCATTTAGCTCCTCTGTCTGCAGGTTTGGCTCATCTTCACTCTTTGAGTCCCCGTCTGGTGCACAAGGATTTTAAAACAGCCAATGTTCTTGTAGATGAAAACTTCATAGCTAAGGTTGCAGATGCGGGAATTCGCAATTTCCTGGGAAGAGTTGATATTGCAGGCTCGTCTTCTCAAGTGGCTGCTGATGAGATGTTCCTTGCTCCAGAGGTCAGAGAATTCAGACAATTTTCAGAAAAAAGTGACGTATATAGTTTTGGTGTATTCCTTCTGGAGTTAGTGAGTGGGCGAGAAGCAAGAGATTTTGCATCTTCTGACTCAAACCAGAACATGGTTGAATGGGTGAAAAATATTCAAGAGGGCAGCAATGTATCGTGCATCATCGATGAGAGGTTGGGGAATAGCTTCACAGGTGAAGCTATGGAAGGGTTGATACAGTTGATCATGCGATGTGTGGAAACTTCGAGTGAGAGGCGACCAAGCATGAGCAATGTGGTAACGGAGCTGGACCGGATAGTTGAGAAAGAGATGAGCTTGACAACAATCATGGGGGAAGGAACTTCTACAGTGATCCCGGGAAGTCAGTTATTTAAAGCAACAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

418

Amino Acids

45.81

Weight (kDa)

6.47

Isoelectric Point (pI)

27.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 80 - 170 3.7e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 83 - 189 1.2e-16 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 218 - 382 4.1e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 221 - 385 1.8e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 659
AccB7I CCANNNNNTGG 1 cut(s) 979
AciI CCGC 3 cut(s) 529, 552, 773
AclWI GGATC 2 cut(s) 131, 1213
AcoI YGGCCR 3 cut(s) 222, 312, 493
AcsI RAATTY 6 cut(s) 247, 266, 347, 518, 777, 866
AcuI CTGAAG 1 cut(s) 117
AcvI CACGTG 1 cut(s) 506
AfaI GTAC 4 cut(s) 363, 446, 452, 485
AfiI CCNNNNNNNGG 1 cut(s) 979
AgsI TTSAA 4 cut(s) 149, 523, 986, 1004
AjnI CCWGG 3 cut(s) 294, 328, 789
Alw21I GWGCWC 1 cut(s) 711
Alw26I GTCTC 3 cut(s) 15, 222, 635
Alw44I GTGCAC 1 cut(s) 707
AlwI GGATC 2 cut(s) 131, 1213
Ama87I CYCGRG 1 cut(s) 1222
AoxI GGCC 4 cut(s) 222, 312, 493, 549
ApaLI GTGCAC 1 cut(s) 707
ApeKI GCWGC 4 cut(s) 17, 35, 830, 1011
ApoI RAATTY 6 cut(s) 247, 266, 347, 518, 777, 866
Asp700I GAANNNNTTC 2 cut(s) 469, 1052
AspS9I GGNCC 2 cut(s) 549, 1156
AsuC2I CCSGG 2 cut(s) 1223, 1224
AsuHPI GGTGA 2 cut(s) 1003, 1073
AvaI CYCGRG 1 cut(s) 1222
AvaII GGWCC 1 cut(s) 1156
BaeGI GKGCMC 1 cut(s) 711
BalI TGGCCA 3 cut(s) 224, 314, 495
BarI GAAGNNNNNNTAC 2 cut(s) 1065, 1097
BbrPI CACGTG 1 cut(s) 506
BbsI GAAGAC 2 cut(s) 163, 810
Bbv12I GWGCWC 1 cut(s) 711
BbvI GCAGC 4 cut(s) 22, 29, 817, 1023
BccI CCATC 2 cut(s) 293, 323
BceAI ACGGC 2 cut(s) 29, 502
BciT130I CCWGG 3 cut(s) 296, 330, 791
BclI TGATCA 1 cut(s) 1089
BcnI CCSGG 2 cut(s) 1223, 1224
BcoDI GTCTC 3 cut(s) 15, 222, 635
BfaI CTAG 3 cut(s) 26, 339, 515
BfmI CTRYAG 4 cut(s) 36, 431, 666, 1212
BfuAI ACCTGC 1 cut(s) 659
BisI GCNGC 5 cut(s) 18, 36, 552, 831, 1012
BlsI GCNGC 5 cut(s) 19, 37, 553, 832, 1013
Bme1390I CCNGG 5 cut(s) 296, 330, 791, 1223, 1224
Bme18I GGWCC 1 cut(s) 1156
BmeT110I CYCGRG 1 cut(s) 1222
BmgT120I GGNCC 2 cut(s) 549, 1156
BmiI GGNNCC 2 cut(s) 43, 550
BmrFI CCNGG 5 cut(s) 296, 330, 791, 1223, 1224
BmsI GCATC 5 cut(s) 75, 394, 760, 965, 1035
BpiI GAAGAC 2 cut(s) 163, 810
BpmI CTGGAG 3 cut(s) 278, 837, 941
Bpu10I CCTNAGC 1 cut(s) 759
BpuEI CTTGAG 2 cut(s) 528, 807
BpuMI CCSGG 2 cut(s) 1223, 1224
Bsa29I ATCGAT 1 cut(s) 1032
BsaAI YACGTR 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 635
BsaJI CCNNGG 2 cut(s) 790, 1222
BsaWI WCCGGW 1 cut(s) 1158
Bsc4I CCNNNNNNNGG 1 cut(s) 979
Bse1I ACTGG 2 cut(s) 474, 496
Bse3DI GCAATG 2 cut(s) 1021, 1144
Bse8I GATNNNNATC 1 cut(s) 65
BseBI CCWGG 3 cut(s) 296, 330, 791
BseCI ATCGAT 1 cut(s) 1032
BseDI CCNNGG 2 cut(s) 790, 1222
BseGI GGATG 1 cut(s) 304
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 1 cut(s) 979
BseMI GCAATG 2 cut(s) 1021, 1144
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 474, 496
BseRI GAGGAG 1 cut(s) 648
BseSI GKGCMC 1 cut(s) 711
BseXI GCAGC 4 cut(s) 22, 29, 817, 1023
BshFI GGCC 4 cut(s) 224, 314, 495, 551
BshVI ATCGAT 1 cut(s) 1032
BsiHKAI GWGCWC 1 cut(s) 711
BsiHKCI CYCGRG 1 cut(s) 1222
BsiSI CCGG 2 cut(s) 1159, 1223
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 1 cut(s) 979
BsmAI GTCTC 3 cut(s) 15, 222, 635
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 1 cut(s) 681
BsmI GAATGC 2 cut(s) 309, 499
BsnI GGCC 4 cut(s) 224, 314, 495, 551
Bso31I GGTCTC 1 cut(s) 635
BsoBI CYCGRG 1 cut(s) 1222
Bsp1286I GDGCHC 1 cut(s) 711
Bsp143I GATC 3 cut(s) 136, 1089, 1218
BspACI CCGC 3 cut(s) 529, 552, 773
BspANI GGCC 4 cut(s) 224, 314, 495, 551
BspCNI CTCAG 1 cut(s) 386
BspDI ATCGAT 1 cut(s) 1032
BspLI GGNNCC 2 cut(s) 43, 550
BspMAI CTGCAG 2 cut(s) 40, 670
BspMI ACCTGC 1 cut(s) 659
BspPI GGATC 2 cut(s) 131, 1213
BspTNI GGTCTC 1 cut(s) 635
BsrDI GCAATG 2 cut(s) 1021, 1144
BsrI ACTGG 2 cut(s) 474, 496
BssECI CCNNGG 2 cut(s) 790, 1222
BssMI GATC 3 cut(s) 136, 1089, 1218
Bst2UI CCWGG 3 cut(s) 296, 330, 791
Bst4CI ACNGT 4 cut(s) 216, 629, 1086, 1216
Bst6I CTCTTC 1 cut(s) 790
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 2 cut(s) 497, 811
BstDEI CTNAG 2 cut(s) 373, 759
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 3 cut(s) 139, 1092, 1221
BstMAI GTCTC 3 cut(s) 15, 222, 635
BstMBI GATC 3 cut(s) 136, 1089, 1218
BstMWI GCNNNNNNNGC 5 cut(s) 14, 41, 548, 674, 764
BstNI CCWGG 3 cut(s) 296, 330, 791
BstSCI CCNGG 5 cut(s) 294, 328, 789, 1221, 1222
BstSFI CTRYAG 4 cut(s) 36, 431, 666, 1212
BstSLI GKGCMC 1 cut(s) 711
BstV1I GCAGC 4 cut(s) 22, 29, 817, 1023
BstV2I GAAGAC 2 cut(s) 163, 810
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 1032
BsuRI GGCC 4 cut(s) 224, 314, 495, 551
BsuTUI ATCGAT 1 cut(s) 1032
BtgZI GCGATG 1 cut(s) 1111
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 3 cut(s) 244, 489, 1221
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 2 cut(s) 497, 811
Cfr13I GGNCC 2 cut(s) 549, 1156
Cfr9I CCCGGG 1 cut(s) 1222
ClaI ATCGAT 1 cut(s) 1032
Csp6I GTAC 4 cut(s) 362, 445, 451, 484
CviAII CATG 5 cut(s) 161, 979, 1093, 1132, 1195
CviQI GTAC 4 cut(s) 362, 445, 451, 484
DdeI CTNAG 2 cut(s) 373, 759
DpnI GATC 3 cut(s) 138, 1091, 1220
DpnII GATC 3 cut(s) 136, 1089, 1218
DraI TTTAAA 2 cut(s) 721, 1240
EaeI YGGCCR 3 cut(s) 222, 312, 493
Eam1104I CTCTTC 1 cut(s) 790
EarI CTCTTC 1 cut(s) 790
Eco31I GGTCTC 1 cut(s) 635
Eco47I GGWCC 1 cut(s) 1156
Eco57I CTGAAG 1 cut(s) 117
Eco72I CACGTG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 1222
EcoRI GAATTC 3 cut(s) 518, 777, 866
EcoRII CCWGG 3 cut(s) 294, 328, 789
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 5 cut(s) 164, 982, 1096, 1135, 1198
FaqI GGGAC 1 cut(s) 681
FatI CATG 5 cut(s) 160, 978, 1092, 1131, 1194
FauI CCCGC 1 cut(s) 766
FauNDI CATATG 2 cut(s) 77, 204
FbaI TGATCA 1 cut(s) 1089
Fnu4HI GCNGC 5 cut(s) 18, 36, 552, 831, 1012
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 5 cut(s) 18, 36, 552, 831, 1012
FspBI CTAG 3 cut(s) 26, 339, 515
GluI GCNGC 5 cut(s) 18, 36, 552, 831, 1012
GsuI CTGGAG 3 cut(s) 278, 837, 941
HaeIII GGCC 4 cut(s) 224, 314, 495, 551
HapII CCGG 2 cut(s) 1159, 1223
Hin1II CATG 5 cut(s) 164, 982, 1096, 1135, 1198
HinfI GANTC 3 cut(s) 436, 694, 965
HpaII CCGG 2 cut(s) 1159, 1223
HphI GGTGA 2 cut(s) 1003, 1073
Hpy166II GTNNAC 3 cut(s) 364, 445, 709
Hpy188I TCNGA 8 cut(s) 121, 136, 390, 568, 863, 872, 883, 964
Hpy188III TCNNGA 6 cut(s) 344, 523, 618, 854, 920, 1004
Hpy8I GTNNAC 3 cut(s) 364, 445, 709
HpyAV CCTTC 4 cut(s) 150, 926, 1067, 1196
HpyCH4III ACNGT 4 cut(s) 216, 629, 1086, 1216
HpyCH4IV ACGT 2 cut(s) 505, 894
HpyCH4V TGCA 8 cut(s) 17, 38, 668, 709, 767, 809, 956, 1026
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 41, 548, 674, 764
HpyF3I CTNAG 2 cut(s) 373, 759
HpySE526I ACGT 2 cut(s) 505, 894
Hsp92II CATG 5 cut(s) 164, 982, 1096, 1135, 1198
Ksp22I TGATCA 1 cut(s) 1089
Kzo9I GATC 3 cut(s) 136, 1089, 1218
LmnI GCTCC 4 cut(s) 41, 661, 856, 1150
Lsp1109I GCAGC 4 cut(s) 22, 29, 817, 1023
LweI GCATC 5 cut(s) 75, 394, 760, 965, 1035
MaeI CTAG 3 cut(s) 26, 339, 515
MaeII ACGT 2 cut(s) 505, 894
MaeIII GTNAC 6 cut(s) 46, 397, 410, 476, 890, 1144
MalI GATC 3 cut(s) 138, 1091, 1220
MboI GATC 3 cut(s) 136, 1089, 1218
MboII GAAGA 7 cut(s) 123, 168, 221, 674, 807, 810, 951
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 711
MlsI TGGCCA 3 cut(s) 224, 314, 495
MluCI AATT 8 cut(s) 247, 266, 347, 518, 777, 784, 866, 875
MluNI TGGCCA 3 cut(s) 224, 314, 495
MlyI GAGTC 2 cut(s) 703, 959
MnlI CCTC 9 cut(s) 23, 158, 268, 352, 669, 850, 1000, 1032, 1113
Mox20I TGGCCA 3 cut(s) 224, 314, 495
MroXI GAANNNNTTC 2 cut(s) 469, 1052
MscI TGGCCA 3 cut(s) 224, 314, 495
MseI TTAA 2 cut(s) 720, 1239
MslI CAYNNNNRTG 2 cut(s) 237, 504
Msp20I TGGCCA 3 cut(s) 224, 314, 495
MspI CCGG 2 cut(s) 1159, 1223
MspR9I CCNGG 5 cut(s) 296, 330, 791, 1223, 1224
Mva1269I GAATGC 2 cut(s) 309, 499
MvaI CCWGG 3 cut(s) 296, 330, 791
MwoI GCNNNNNNNGC 5 cut(s) 14, 41, 548, 674, 764
NciI CCSGG 2 cut(s) 1223, 1224
NdeI CATATG 2 cut(s) 77, 204
NdeII GATC 3 cut(s) 136, 1089, 1218
NlaIII CATG 5 cut(s) 164, 982, 1096, 1135, 1198
NlaIV GGNNCC 2 cut(s) 43, 550
NmuCI GTSAC 3 cut(s) 46, 476, 890
PctI GAATGC 2 cut(s) 309, 499
PdmI GAANNNNTTC 2 cut(s) 469, 1052
PfeI GAWTC 1 cut(s) 436
PflMI CCANNNNNTGG 1 cut(s) 979
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 5 cut(s) 19, 37, 553, 832, 1013
PleI GAGTC 2 cut(s) 702, 959
PmaCI CACGTG 1 cut(s) 506
PmlI CACGTG 1 cut(s) 506
PpsI GAGTC 2 cut(s) 702, 959
Ppu21I YACGTR 1 cut(s) 506
Psp6I CCWGG 3 cut(s) 294, 328, 789
PspCI CACGTG 1 cut(s) 506
PspGI CCWGG 3 cut(s) 294, 328, 789
PspN4I GGNNCC 2 cut(s) 43, 550
PspPI GGNCC 2 cut(s) 549, 1156
PstI CTGCAG 2 cut(s) 40, 670
PsuI RGATCY 1 cut(s) 136
RsaI GTAC 4 cut(s) 363, 446, 452, 485
RsaNI GTAC 4 cut(s) 362, 445, 451, 484
RseI CAYNNNNRTG 2 cut(s) 237, 504
SaqAI TTAA 2 cut(s) 720, 1239
SatI GCNGC 5 cut(s) 18, 36, 552, 831, 1012
Sau3AI GATC 3 cut(s) 136, 1089, 1218
Sau96I GGNCC 2 cut(s) 549, 1156
SchI GAGTC 2 cut(s) 703, 959
ScrFI CCNGG 5 cut(s) 296, 330, 791, 1223, 1224
SduI GDGCHC 1 cut(s) 711
SfaNI GCATC 5 cut(s) 75, 394, 760, 965, 1035
SfcI CTRYAG 4 cut(s) 36, 431, 666, 1212
SinI GGWCC 1 cut(s) 1156
SmaI CCCGGG 1 cut(s) 1224
SmiMI CAYNNNNRTG 2 cut(s) 237, 504
SmlI CTYRAG 2 cut(s) 543, 822
SmoI CTYRAG 2 cut(s) 543, 822
Sse9I AATT 8 cut(s) 247, 266, 347, 518, 777, 784, 866, 875
SsiI CCGC 3 cut(s) 529, 552, 773
SspI AATATT 2 cut(s) 394, 1000
SspMI CTAG 3 cut(s) 26, 339, 515
StyD4I CCNGG 5 cut(s) 294, 328, 789, 1221, 1222
TaaI ACNGT 4 cut(s) 216, 629, 1086, 1216
TaiI ACGT 2 cut(s) 508, 897
TaqI TCGA 2 cut(s) 1032, 1112
TasI AATT 8 cut(s) 247, 266, 347, 518, 777, 784, 866, 875
TatI WGTACW 1 cut(s) 450
TauI GCSGC 1 cut(s) 554
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 2 cut(s) 720, 1239
Tru9I TTAA 2 cut(s) 720, 1239
TscAI CASTG 3 cut(s) 244, 496, 1221
TseFI GTSAC 3 cut(s) 46, 476, 890
TseI GCWGC 4 cut(s) 17, 35, 830, 1011
Tsp45I GTSAC 3 cut(s) 46, 476, 890
TspDTI ATGAA 4 cut(s) 191, 446, 742, 759
TspGWI ACGGA 1 cut(s) 1163
TspMI CCCGGG 1 cut(s) 1222
TspRI CASTG 3 cut(s) 244, 496, 1221
Van91I CCANNNNNTGG 1 cut(s) 979
VneI GTGCAC 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 1156
XapI RAATTY 6 cut(s) 247, 266, 347, 518, 777, 866
XmaI CCCGGG 1 cut(s) 1222
XmnI GAANNNNTTC 2 cut(s) 469, 1052
XspI CTAG 3 cut(s) 26, 339, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.